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EN
The efects were studied of the quality and amount of dietary fat on the pattern of fatty acids in the lipids of the serum and certain tissues (adipose fat, perirenal fat, liver, heart, testes) of guined pigs during experimentally induced hypercholesterolemia. During 12 weeks the animals received experimental diets containg 10% or 20% of energy from animal fats (butter, lard 2:3), sunflower oil or low-erucic rapeseed oil. Two control groups were chosen, receiving diets for animals without cholesterol or with 0.1% cholesterol added. The addition of cholesterol to the diet raised the content of the essential unsaturated fatty acids and polyunsaturated fatty acids in the adipose fat and hepatic lipids, and decreased their content in myocardial lipids. In testicular lipids changes were noted in the synthesis of long-chain polyunsaturated fatty acids. The addition of vegetable fats to the diet increased the content of linoleic acid in these tissues with a simultaneous decrease of arachidonic acid synthesis. It may be supposed that there is an upper range of arachidonic acid synthesis in the lipids of the studied tissues independent of dietary EFA and PUFA value.
PL
Oznaczano wpływ ilości i jakości tłuszczu na skład kwasów tłuszczowych lipidów surowicy krwi i wybranych tkanek (tłuszcz zapasowy, wątroba, serca, jądra) świnek morskich w warunkach doświadczalnej hipercholesterolemii. Zwierzętom podawano w ciągu 12 tygodni diety doświadczalne zawierające 10% i 20% energii z tłuszczu zwierzęcego (masło, smalec 2; 3), oleju słonecznikowego lub oleju rzepakowego bezerukowego. Zastosowano dwie grupy kontrolne otrzymujące diety hodowlane bez cholesterolu oraz z dodatkiem 0.1% cholesterolu. Stwierdzono, że dodatek cholesterolu do diety hodowlanej wywołuje wzrost zawartości NNKT i PUFA w tłuszczu zapasowym i lipidach wątroby, przy jednoczesnym spadku tych kwasów w lipidach serca. W lipidach jąder stwierdzono zmiany w sytezie długołańcuchowych wielonienasyconych kwasów tłuszczowych. Dodatek do diety tłuszczów roślinnych zwiększa zawartość kwasu linolowego w badanych tkankach, przy jednoczesnym hamowaniu syntezy kwasu arachidonowego. Można przypuszczać, że istnieje określony górny limit syntezy kwasu arachidonowego w lipidach badanych tkanek niezależnie od ilości NNKT i PUFA w diecie.
EN
The objective of this study was to evaluate the behaviour of fibre in the digestive tract on the basis of the passage kinetics of forage and concentrate particles in cows fed different omega-6 fatty-acid sources. The scientific hypothesis of this study was that omega-6 fatty acids do not interfere with the digestion of fibre in the diets of dairy cows. Five primiparous lactating Holstein cows were used in the experiment. The experimental diets were: control (C), ground soyabean (GS), cottonseed (CS), soyabean oil (SO), calcium salts of fatty acids (CSFA). The global mean estimates for the parameters of passage rate (γ) were 0.038 and 0.055 h–1 for forage and concentrate, respectively. The only significant effect with respect to the passage rate was a high negative correlation between the concentrate passage rate and dry matter intake. There was less undegradable neutral detergent fibre (NDF) in treatments without added lipid. Dietary supplementation with lipid sources does not alter the kinetic parameters of roughage and concentrate particle passage or in vitro NDF degradation. Sources of omega-6 fatty acids do not alter the rumen degradation and transit of fibre.
EN
Polyunsaturated fatty acids (PUFAs, LA and ALA) are not synthesized in mammals in the absence of their essential fatty acid precursors. However, hibernating animals and animals sleeping through the winter need sufficiently high amounts of these acids. The Eurasian badger does not hibernate, but sleeps over winter. In the autumn the body weight of adult badgers increases even twofold, since they put on fat before the winter. Fat is deposited primarily in the subcutaneous layer of adipose tissue, and much less commonly in the muscles, liver and around the intestines. The percentage composition of fatty acids (Saturated fatty acids-SFAs, monounsaturated fatty acids-MUFAs, PUFAs) is different in particular types of tissue. The lipids isolated from depot adipose tissues (suet, subcutaneous, perirenal, periintestinal fat) are dominated by monounsaturated fatty acids (on average 41.25%), followed by saturated fatty acids (33.53%). Polyun­saturated FAs have the lowest proportion in this groups of tissues, on average 17.75% of total FAs. On the other hand, liver lipids contain over 44% PUFAs. The fatty acid composition of lipids in badgers tissue includes considerable quantities of essential unsaturated n-6 and n-3 fatty acids of great pharmacological significance.
EN
Lipid peroxidation is a process involving the oxidation of polyunsaturated fatty acids (PUFAs), which are basic components of biological membranes. Reactive electrophilic compounds are formed during lipid peroxidation, mainly α,β-unsaturated aldehydes. These compounds yield a number of adducts with DNA. Among them, propeno and substituted propano adducts of deoxyguanosine with malondialdehyde (MDA), acrolein, crotonaldehyde and etheno adducts, resulting from the reactions of DNA bases with epoxy aldehydes, are a very important group of adducts. The epoxy aldehydes are more reactive towards DNA than the parent unsaturated aldehydes. The compounds resulting from lipid peroxidation mostly react with DNA showing both genotoxic and mutagenic action; among them, 4-hydroxynonenal is the most genotoxic, while MDA is the most mutagenic. DNA damage caused by the adducts of lipid peroxidation products with DNA can be removed by the repairing action of glycosylases. The formed adducts have been hitherto analyzed using the IPPA (Imunopurification-32P-postlabelling assay) method and via gas chromatography/electron capture negtive chemical ionization/mass spectrometry (GC/EC NCI/MS). A combination of liquid chromatography with electrospray tandem mass spectrometry (LC/ES-MSMS) with labelled inner standard has mainly been used in recent years.
EN
Lipids obtained from the muscular and adipose tissues of the European beaver were isolated by the modified Folch method. Fatty acids were converted to methyl esters and separated by high-resolution gas chromatography (HR-GC). The content and composition of beaver fat depended on the sex of a given animal. The adipose subcutaneous tissue of the female contained the most fat (approximately 70.5%). The fat content of muscular tissue was very low in both male and female beavers. Adipose tissue lipids of the beaver contained fatty acids ranging in chain length from 12 to 22 carbon atoms. Polyunsaturated fatty acids have the highest proportion in of total FA content of adipose tissue in the beaver, which distinguishes these lipids from the lipids of adipose tissues in other mammals. The results of the present study also confirmed a unique FA composition in the tail fat of the beaver, including a very high content of alpha-linolenic acid (ALA, 18:3 n-3) (on average 20.0%) and the sum of n-3 fatty acids (on average 20.45%). In addition, a very low content of the sum of saturated fatty acids (on average 14.93%) was observed, and an extremely low content, as for animal fat, of palmitic acid 16:0 (on average 10.53%).
PL
Kwasy tłuszczowe z grupy omega-3 występują w znacznych ilościach m.in. w rybach, olejach rybich i roślinnych. Współczesna dieta charakteryzuje się zbyt dużym spożyciem tłuszczów nasyconych i kwasów omega-6, a zbyt małym kwasów omega-3. Wzrost spożycia kwasów omega-3 ma wpływ na obniżenie zachorowalności na choroby układu krążenia, pracę mózgu, nerek, narządów przewodu pokarmowego. Kwasy omega-3 działają również przeciwzapalnie. Niedoborom kwasów omega-3 można przeciwdziałać dietą oraz wprowadzając suplementację oraz wzbogacanie żywności.
EN
Omega-3 fatty acids are present in significant amount in fishes, fish and some vegetable oils. Modern diet is characterized by high consumption of saturated fatty acids and omega-6 fatty acids and too low intake of omega-3 fatty acids. Higher intake of omega-3 fatty acids lowers the frequency of morbidity on cardiovascular dieses, brain, kidney and digestive tract and also anti-inflammation. Deficiencies of omega-3 might be counteracted by diet and by supplementation and food enriching by omega-3 fatty acids.
EN
In a randomly selected group of 444 men and women divided into three age groups: 35-44 years, 45-64 years and 55-64 years, the composition of fatty acids in total serum lipids was determined by gas chromatography, with particular reference to EFA (linoleic, linolenic, arachidonic acids) and the P:S index. The results showed that the levels of EFA and PUFA (all unsaturated fatty acids) were significantly higher in women than in men in this population. The mean EFA value in women was 31.3% and in men 29.3%. The PUFA level was 33.7% and 31.7% respectively. Similarly, the P:S index in women was significantly higher - 1.02 than in men - 0.94. In the light of these results, it may be assumed, that the serum EFA level in the studied subjects points to an intake of those acids covering up to 3% of the total energy of the daily food ration.
PL
Badanie składu kwasów tłuszczowych przeprowadzono ogółem u 444 mężczyzn i kobiet w trzech grupach wiekowych: 35-44 lat, 45-54 lat i 55-64 lat. Zawartość poszczególnych kwasów tłuszczowych w lipidach całkowitych surowicy krwi oznaczono metodą chromatografii gazowej.Z badań wynika, że zawartość NNKT (kwas linolowy 18:2, n-6; kwas linolenowy - 18:3, n-3 oraz kwas arachidonowy - 20:4, n-6) i wszystkich kwasów wielonienasyconych - P średnio w całej populacji kobiet jest statystycznie istotnie (przy p≤0,05) wyższa niż u mężczyzn i wynosi: u kobiet NNKT - 31,31%, u mężczyzn 29,31%; u kobiet P - 33,73%, u mężczyzn - 31,74%. Podobnie wskaźnik P:S u kobiet jest wyższy statystycznie istotnie niż u mężczyzn i wynosi 1,02 w porównaniu z 0,94. Na podstawie wcześniejszych podobnych badań można ocenić udział NNKT w dostarczaniu energii w dziennych racjach pokarmowych badanych mężczyzn i kobiet na poziomie około 3%.
PL
Przedstawiono możliwość zastosowania estrów etylowych wielonienasyconych kwasów tłuszczowych z grupy omega-3 o wysokiej zawartości kwasu alfa-linolenowego (ALA) jak składnika dodatków paszowych dla krów mlecznych i owiec. Wykazano, że dodatek nie spowodował zmian w składzie podstawowym mleka od badanych zwierząt, a spowodował korzystne zmiany w profilu kwasów tłuszczowych mleka. W obu przypadkach zaobserwowano korzystny wzrost udziału kwasów nienasyconych (o ok. 22% w mleku owczym i ok. 20% w mleku krowim). Zawartość kwasu ALA wzrosła w wyniku suplementacji odpowiednio o 42 i 59% w mleku owiec i krów. Wykazano, że mleko pochodzące od zwierząt żywionych dietą z dodatkiem kwasu alfa-linolenowego może być cennym produktem zaliczanym do żywności funkcjonalnej.
EN
Et esters of polyunsatd. omega-3 fatty acids of high alpha-linolenic acid (I) were used as a component of fodder supplements for dairy cows and sheep. The addn. did not result in any changes in basic compn. of milk from the examd. animals, but profitably modified profil of the milk fatty acids. An increase in unsatd. fatty acids content was obsd. in both cases (by 22% in sheep, and by 20% in cow milks). The content of I increased by 42 and 59% in sheep and cows milks, resp.
EN
The Flathead mullet (Mugil cephalus) is a mullet of the genus Mugil in the family Mugilidae, found in coastal tropical, subtropical and estuarine water worldwide. Lipid was extracted from the muscle of Flathead mullet (Mugil cephalus) by solvent extraction method and characterized with respect to various chemical e.g. saponification value, saponification equivalent value, acid value, iodine value, peroxide value, ester value, thicyanogen value, unsaponifiable matter, Henher value, Polenske value, Reichert-Meissl value etc properties and compared with those of different oils. Palmitic acid, Stearic acid and Myristic acid were observed at fatty acid composition of the sample by chromatographic examination. Percentages of protein and mineral (N, P, K, Ca) in the muscle of Flathead mullet were studied by modified Kjeldahl method.
EN
The aim of the study was to evaluate physical traits and fatty acids profile of ostrich meat enriched in n3 fatty acids as affected by refrigerated storage (for 14 days) and type of packaging (VAC vs. skin-packaging – SP). During refrigerated storage time drip loss after 7 days was significantly (P<0.001) higher in VAC as compared to SP samples. No significant differences in the SFA content in meat during storage in both types of packaging types recorded. Although there were no significant differences (P=0.067), a tendency for higher MUFA values was observed during storage in VAC packed meat. A significant decrease (P<0.05) in the content of PUFA after 7 and 14 days of storage was also observed in VAC packed meat as compared to fresh meat, whereas, when skin packaging was used, no differences in the PUFA concentration were found. Considering this, the SP can be recommended packaging for ostrich meat industry.
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