Wyniki wyszukiwania - Biblioteka Nauki

1

Antibacterial peptides of the moth Galleria mellonella

100%

Mak P. , Chmiel D. , Gacek G. J.

Acta Biochimica Polonica

|

2001

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tom 48

|

nr 4

1191-1195

EN

The work describes purification and biochemical characterization of two inducible antimicrobial peptides from the hemolymph of Galleria mellonella. The peptides were isolated by a sequence of reversed-phase chromatography steps from the hemolymph of larvae immunized with viable bacteria. The first peptide is a member of the cecropin family while the second one is rich in proline residues and has a unique sequence.

2

Isolation of faecal Enterococcus faecium strains from rabbits and their sensitivity to antibiotics and ability to bacteriocin production

100%

Simonova M. , Laukova A.

Bulletin of the Veterinary Institute in Puławy

|

2004

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tom 48

|

nr 4

3

Production of dry divercin preparations by spray drying and lyophilization

72%

Dembczynski R. , Lubbe P. , Bialas W. , Sip A. , Jankowski T. , Grajek W. , Marecik R. , Cyplik P.

Electronic Journal of Polish Agricultural Universities. Series Food Science and Technology

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2008

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tom 11

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nr 2

4

Evidence for probiotic potential of a capsular-producing Streptococcus thermophilus CHCC 3534 strain

72%

Khalil R.

Polish Journal of Microbiology

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2009

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tom 58

|

nr 1

49-55

EN

The purpose of this research was to evaluate the probiotic potential of an capsulated Streptococcus thermophilus CHCC 3534 strain. The strain tolerates 0.4% oxgall (bile) and was sufficiently resistant to pH as low as 2.5 for 3 hours of exposure. The strain demonstrated high adherence to human intestinal mucus, and showed unique resistance to different antibiotics. Crude extracts of S. thermophilus CHCC 3534 contained a diffusible antimicrobial compound "bacteriocin" with a broad spectrum that inhibited the growth of closely related lactic acid bacteria and a number of food spoilage bacteria including Salmonella typhimurium and Staphylococcus aureus. The bacteriocin was heat stable, resistant to pH, inactivated by proteolytic enzymes, and resistant to á-amylase and lipase. A SDS-PAGE analysis of the partially purified bacteriocin revealed one component with a molecular weight ranging from 14.4 to 18.4 kDa. The strain may have industrial significance and represents an interesting candidate for use in biopreservation, probiotic food formulations and in the control of spoilage caused by food borne pathogens.

5

Sensitivity of Shigella flexneri and Escherichia coli bacteria to bacteriophages and to colicins, lost or established by the acquisition of R plasmids

72%

Smarda J. , Doroszkiewicz W. , Lachowicz T. M.

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1990

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tom 39

|

nr 1-2

6

Wpływ diwercyny i ogrzewania środowiska na wzrost bakterii z rodzaju Listeria

72%

Sip A. , Grajek W.

Acta Scientiarum Polonorum. Biotechnologia

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2002

|

tom 01

|

nr 1-2

7

Development of synbiotics with inulin, palatinose, alpha-cyclodextrin and probiotic bacteria

72%

Pranckute R. , Kaunietis A. , Kuisiene N. , Citavicius D.

Polish Journal of Microbiology

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2014

|

tom 63

|

nr 1

EN

Success in creating a synbiotic depends on compatibility between the chosen components – prebiotic and probiotic. In this work the interactions between Lactobacillus sp. strains isolated from yogurts and type strains of Lactobacillus sp. and Lactococcus sp., and the dependence of their growth and antibacterial activity on three oligosaccharides (OS) – palatinose, inulin and α-cyclodextrin were investigated. All isolated lactobacilli produce antibacterial compounds, which possibly are the bacteriocins of Lactobacillus casei ATCC334 strain. Results of growth analysis with different OS revealed that part of lactobacilli isolated from yogurts can effectively ferment inulin and may be used for the development of synbiotics. Palatinose and Lactobacillus acidophilus could be used as symbiotics with effective antibacterial activity. One of the types of Lactococcus sp. strains can assimilate palatinose and α-cyclodextrin, so they both can be used as components of synbiotics with the investigated lactococci. Results of this analysis suggest that the investigated isolated and type strains of Lactobacillus sp. and Lactoccocus sp. can be useful as probiotics in the development of synbiotics. Together with prebiotics – palatinose, inulin and α-cyclodextrin, the synbiotics, which could regulate not only the growth of beneficial bacteria in the gastrointestinal tract, but also their antibacterial activity, can be created.

8

Salivaricin B gene - its localization and RFLP analysis in two potentially probiotic Lactobacillus salivarius strains

72%

Kizerwetter-Swida M. , Binek M.

Bulletin of the Veterinary Institute in Puławy

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2010

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tom 54

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nr 4

EN

The study has provided data on two Lactobacillus salivarius strains of poultry origin. The strains were investigated for the presence of genes encoding a bacteriocin: salivaricin B, and their localisation in chromosome or plasmid DNA. Specific primers were used to amplify a 224 bp fragment of salivaricin B gene (salB). RFLP analysis of PCR products revealed two DNA fragments of the predicted sizes upon digestion with Ndel and Xapl. Analysis with SspI allowed obtaining variability in two fragments in comparison to computer analysis of both strains, suggesting their divergency. It may be assumed that the amplified DNA fragments of salB gene share great, but not complete similarity to the previously described sequence of salB gene. Two examined strains showed different plasmid profiles; however bands of similar sizes were seen in both profiles. Genes responsible for salivaricin production are located on chromosomal DNA. Properties of these strains, in particular the presence of genes encoding bacteriocin production, imply that they may be used as potential probiotics for poultry.

9

The effects of Carnobacterium divergens AS7 bacteriocin on gastrointestinal microflora in vitro and on nutrient retention in broiler chickens

72%

Jozefiak D. , Sip A. , Kaczmarek S. , Rutkowski A.

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2010

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tom 19

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nr 3

EN

The present study was undertaken to evaluate the effects of divercin, a bacteriocin produced by lactobacilli strain Carnobacterium divergens AS7, on the microflora status under in vitro conditions and on nutrient retention and nitrogen-corrected apparent metabolizable energy (AMEN ) of divercin in an in vivo trial on broiler chickens. Low (DL) 200 AU·ml1 (0.05% of the liquid divercin prepatation), and high (DH) 1600 AU·ml-1(0.4% of the liquid divercin prepatation) doses of divercin were used in both trials. In the in vitro trial divercin at concentration, 1600AU ml-1 of divercin had stronger antibacterial effects as compared with 200 AU·ml-1. In the crop and ileal digesta, the DH treatment was characterized by the lowest lactic acid bacteria (LAB) and coliform bacteria counts (0.4-0.8 log cycle reduction). There were no differences in nutrient retention between treatments. Salinomycin and divercin supplementation tended to increase fat digestibility and N retention. However, the highest AMEN were obtained in the DL treatment. The results of both studies show positive effects of divercin in terms of reduction of microbial populations isolated from the gastrointestinal tract (GIT) of broiler chickens as well as improvement in AMEN. The presented data may suggest that bacteriocin derived from Carnobacterium divergens AS7 could play a role in controlling the microbial ecosystem in the broiler chicken GIT.

10

Synergy between novel antimicrobials and conventional antibiotics or bacteriocins

72%

Wolska K. I. , Grzes K. , Kurek A.

Polish Journal of Microbiology

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2012

|

tom 61

|

nr 2

EN

Due to the alarming spread of resistance to classic antimicrobial agents, innovative therapeutic methods to combat antibiotic-resistant bacterial pathogens are urgently required. This minireview examines the enhancement of antibiotic efficacy by their combination with new antimicrobials, such as plant-derived compounds, metal ions and nanoparticles and bacteriophage lytic enzymes. The mechanisms of the observed synergy are also described. The promising results of basic research indicate that in future, combined therapy may be applied in human and veterinary medicine, agriculture and the food industry to combat bacterial pathogens.

11

Antibacterial peptides of the moth Galleria mellonella

72%

Mak P. , Chmiel D. , Gacek G. J.

Acta Biochimica Polonica

|

2001

|

tom 48

|

nr 4

EN

The work describes purification and biochemical characterization of two inducible antimicrobial peptides from the hemolymph of Galleria mellonella. The peptides were isolated by a sequence of reversed-phase chromatography steps from the hemolymph of larvae immunized with viable bacteria. The first peptide is a member of the cecropin family while the second one is rich in proline residues and has a unique sequence.

12

Antibacterial effect of bacteriocins isolated from Lactobacillus strains on selected gram-negative pathogenic rods

72%

Laniewska-Moroz L. , Warminska-Radyko I. , Dajnowiec M. , Gradzka J.

|

nr 3

EN

The aim of the present paper was to determine the effect of bacteriocins produced by Lactobacillus strains isolated from pickled vegetables on Gram-negative pathogenic rods. The antibacterial effect of bacteriocins was determined with the diffusion method, employing the well technique. Incubation was carried out for 24 hours, under the optimum conditions for test strains, and then growth inhibition zones around wells were observed. The results obtained show that individual Lactobacillus strains produce antibacterial substances - bacteriocins, characterized by different activity towards selected strains of Yersinia enterocolitica, Salmonella E. coli O 157.H7 and E. coli K88. Test strains were more sensitive to bacteriocins synthesized by Lactobacillus plantarum, as their growth was inhibited at the activity level 4-32 AU/mL. Bacteriocins of Lactobacillus brevis strains inhibited their growth at 128 and 256 AU/mL.

PL

Celem pracy było określenie wpływu bakteriocyn szczepów Lactobacillus wyizolowanych z kiszonych warzyw na gramujemne pałeczki chorobotwórcze. Aktywność antybakteryjną badano metodą dyfuzyjną, stosując technikę studzienkową. Inkubację prowadzono przez 24 godziny w warunkach optymalnych dla szczepów testowych, po czym obserwowano strefy zahamowania wzrostu wokół studzienek. Na podstawie uzyskanych wyników stwierdzono, że poszczególne szczepy Lactobacillus wytwarzały substancje antybakteryjne o charakterze bakteriocyn, różniące się aktywnością wobec wybranych szczepów Yersinia enterocolitis, Salmonella E. coli O 157:H7 і E. coli К88. Szczepy testowe były bardziej wrażliwe na bakteriocyny syntetyzowane przez Lactobacillus plantarum, ponieważ hamowanie ich wzrostu następowało przy aktywności 4-32AU/mL, podczas gdy bakteriocyny szczepów Lactobacillus brevis hamowały ich wzrost przy aktywności 128 i 256 AU/mL.

13

Production of bacteriocin by Carnobacterium divergens grown in culture media containing hydrolysates of whey, casein and malt roots

72%

Grajek W. , Bobowicz-Lassocinska T. , Sip A.

Polish Journal of Food and Nutrition Sciences

|

1996

|

tom 05

|

nr 4

EN

A bacteriocin divercin was produced in batch cultures by Carnobacterium divergens. The bacteria were grown in the liquid MRS media containing enzymatic hydrolysates of whey, casein or malt roots as organic nitrogen sources. The divercin production was greatly affected by the medium composition. The highest divercin activity (12,800 AU/mL), measured by the critical dilution assay using C. piscicola as an indicatory microorganism, was obtained in the medium containing whey, casein hydrolysates, and glucose. Omission of glucose in this medium delayed the divercin biosynthesis only for 5 h. The medium developed was more efficient than the MRS medium used commonly for the bacteriocin production.

PL

W pracy przedstawiono wyniki badań nad produkcją bakteriocyny divercyny w hodowlach okresowych Carnobacterium divergens. Bakterie hodowano w pożywkach płynnych zawierających hydrolizaty enzymatyczne białek serwatkowych, kazeiny i białek z korzonków słodowych (tab. 1, 2,3). Stwierdzono, że produkcja divercyny jest silnie determinowana przez skład chemiczny pożywki (rys. 1, tab 3). Największą aktywność bakteriocyny, mierzoną metodą krytycznych rozcienczeń wobec C. piscicola jako organizmu wskaźnikowego, wykryto w pożywce zawierającej hydrolizat serwatki, hydrolizat kazeiny i glukozę. Wynosiła ona 12.800 JA/mL (tab. 5). Pominięcie glukozy w składzie pożywki nie obniżyło aktywności divercyny tylko opóźniło jej biosyntezę o 5 godzin.

14

Testing of probiotic and bacteriocin-producting lactic acid bacteria towards Eimeria sp.

58%

Strompfova V. , Laukova A. , Marcinakova M. , Vasilkova Z.

|

tom 13

|

nr 2

15

Bacteriocinogenicity, sensitivity and resistance of Yersinia enterocolitica strains isolated from swine

58%

Jakubczak A. , Bukowski K.

Acta Academiae Agriculturae ac Technicae Olstenensis. Veterinaria

|

1996

|

tom 24

87-92

EN

The reference strain Yersinia enterocolitica 0:3 - 44 as well as 12 other strains isolated From swine at the Veterinary Hygiene Research Station (VHRS) in Łomża were studied. At 25°C for 24 h the reference strain produced an antibiotic-like substance - bacteriocin. Its amount contained in 4.2 ml in TSB broth medium inhibits the growth of sensitive tested strains, while a concentration of 4,5 ml inhibits also the resistant ones. The bacteriocin synthesized by the reference strain is sensitive to proteolytic enzymes- papain and trypsin when undiluted or only at concentration of 10-1.

PL

Badano referencyjny szczep Y. enterocolitica 0:3 - 44 i szczepy wyizolowane od świń w Zakładzie Higieny Weterynaryjej w Łomży. Szczep referencyjny w temp. 25°C, po 24 godz. inkubacji na podłożu TSB wytwarzał substancję bakteriocynopodobną. Wzrost badanych szczepów wrażliwych hamowało 4,2 ml supernatantu zawierającego bakteriocynę w podłożu TSB zaś opornych - zawartość 4,5 ml. Bakteriocyna jest wrażliwa na enzymy proteolityczne: papainę i trypsynę nie rozcieńczone lub najwyżej w rozcieńczeniu 10-krotnym.

16

Biosynthesis of divercin by Carnobacterium divergens AS7 in continuous high cell density cultures

58%

Sip A. , Grajek W.

Electronic Journal of Polish Agricultural Universities. Series: Biotechnology

|

2003

|

tom 06

|

nr 1

17

Aktywnosc antagonistyczna bakterii fermentacji mlekowej z gatunku Lactobacillus plantarum

58%

Kraszewska J. , Wzorek W. , Sztando E. , Raczynska-Cabaj A.

Acta Scientiarum Polonorum. Technologia Alimentaria

|

2005

|

tom 04

|

nr 1

PL

Celem pracy było określenie antagonlstycznych właściwości 6 szczepów z gatunku Lactobacillus plantarum, używanych przez autorów w badaniach nad bioaktywnym napojem słodowym. Zastosowano metodę studzienkową i słupkową oraz zbadano możliwość produkcji przez badane szczepy nadtlenku wodoru. Antagonizm bakteryjny określano w stosunku do wybranych bakterii gram-dodatnich i gram-ujemnych oraz drożdży. Stwierdzono, że badane szczepy nie mają zdolności produkcji nadtlenku wodoru. Wszystkie szczepy L. plantarum hamują wzrost stosowanych bakterii wskaźnikowych, a powstałe strefy zahamowania wzrostu są prawdopodobnie wynikiem produkcji kwasu mlekowego przez bakterie antagonistyczne. Badane bakterie mlekowe nie wykazują inhibicji wzrostu drożdży. Zaobserwowano również zróżnicowany poziom wrażliwości wśród mikroorganizmów wskaźnikowych należących do tego samego rodzaju lub gatunku. W metodzie słupkowej stwierdzano większe strefy zahamowania wzrostu niż w metodzie studzienkowej.

EN

The aim of this study was to determine the antagonistic properties of six Lactobacillus plantarum strains against Gram-positive, Gram-negative bacteria and chosen yeast strain. Agar slab method and well diffusion assays were used in this study. The ability of bacteria to produce the hydrogen peroxide was investigated. All the chosen L. plantarum strains are able to inhibit used indicator bacterial strains. It was concluded that there appeared lack of bacterial growth zones and it resulted in lactic acid production. The chosen lactic acid bacteria did not inhibit growth of C. albicans yeast. Better results - bigger inhibition zones were obtained using agar slab method then well diffusion assays. All the L. plantarum strains did not reveal production hydrogen peroxide.

18

Dzialanie bakteriocyn wytwarzanych przez bakterie z rodzaju Campylobacter

58%

Jakubczak A. , Bukowski K.

Acta Academiae Agriculturae ac Technicae Olstenensis. Veterinaria

|

1996

|

tom 24

81-86

PL

Wśród 69 wyizolowanych od bydła szczepów Campylobacter stwierdzono dwa szczepy bakteriocynogenne BI i B2, należące do podgatunku sputorum, 20 bakteriocynowrażliwych i 48 bakteriocynoopornych. Bakteriocyny działały na 15 szczepów podgatunku sputorum i 5 szczepów podgatunku bubulus z tym, że te szczepy były w większości wrażliwe na jedną z bakteriocyn, niektóre na obie. U szczepów opornych podgatunków bubulus i sputorum zaobserwowano różny stopień oporności na badane bakteriocyny, niektóre były oporne na оbiе bakteriocyny.

EN

69 Campylobacter strains were isolated from cattle. Two of the strains, B1 and B2, belonging to sputorum subspecies, turned out to be bacteriocinogenic, 20 of them were bacteriocinosensitive, and 48 - bacteriocinoresistant. Bacteriocins had certain effects on 15 strains of sputorum subspecies and 5 strains of bubulus subspecies. Most of the strains were sensitive to one of bacteriocins, only some - to both. In the case of bacteriocinosensitive strains of the above-mentioned subspecies, different levels of resistance were observed; a few of them proved ; resistant to both bacteriocins.

19

Substances with antibacterial activity in edible films - a review

58%

Krasniewska K. , Gniewosz M.

Polish Journal of Food and Nutrition Sciences

|

2012

|

tom 62

|

nr 4

EN

This article is an overview of literature addressing edible fi lms and substances introduced to fi lms in order to impart them the antimicrobial activity. It describes natural polymers applied for the production of food packages and active substances of natural origin added to them, including: bacteriocins, enzymes, oils, and plant extracts. Further discussion refers to chitosan – a polysaccharide used for fi lm formation and characterised by strong antibacterial and antimycotic properties.

20

Stabilnosc preparatow bakteriocyny bakterii Carnobacterium divergens AS7 podczas przechowywania

58%

Sip A. , Grajek W.

Acta Scientiarum Polonorum. Biotechnologia

|

2006

|

tom 05

|

nr 1-2

75-85