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EN
The goal of this study was to analyse the qualitative changes occurring in oil during microwave heating. The subject of the study was Kujawski oil, manufactured by ZT Kruszwica, and purchased retail. Oil samples were heated in an RM 800 microwave reactor. Microwaves at a power of 200, 400, 600 and 800 W were used. The heating duration for the oil samples ranged from 3 to 30 minutes. As a result of heating, the oils were observed to increase in temperature depending on the microwave power. This resulted in oxidative changes in the oil samples, as evidenced by the increase in the values on the peroxide, anisidine and Totox index. The values for these parameters increased over time in all the analysed oil samples. In oils heated by a microwave power of 400–800W, changes in the anisidine value and Totox index over time are illustrated by increasing convex curves. The higher the power of the microwave, the higher the value of this parameter observed in the samples. The highest anisidine value, equal to 143.8, was found in oil samples heated by 800 W microwaves for 30 minutes. The highest Totox value – 155.4 mEq O2/kg – occurred in oil samples heated by 800 W microwaves for 30 minutes.
EN
The article presents the results of the 2014–2015 studies on seed yield and the physicochemical and fatty acid composition of Akmay grade safflower oil cultivated at the experimental plot of LLC "Farmer 2002", Astrakhan district, Akmola region, Kazakhstan. The peroxide and acid number was determined with the titrimetric method with visual indication, and the iodine number was calculated as well. The fatty acid composition of oil was studied using the gas chromatography method. Up to 12 fatty acids were detected in the composition of total lipids. The highest content of fatty acids in the composition of safflower seed oil triglycerides was observed in unsaturated linoleic acid (82.7%). During the years of research, the weather conditions greatly influenced the seed yield and the physicochemical quality of safflower oil. Conclusions about future use of this variety as raw material for producing edible vegetable oil were drawn.
EN
The aim of the study was to evaluate the fatty acids composition of storage fat of slaughter horses. Samples of cervical and peri-intestinal fat taken from horses of different genders and weights in a slaughterhouse were used as the study material. The fat was esterified by the method described by Peisker. Separation and analysis of the fatty acids was conducted by gas chromatography with a flame ionisation detector. The samples were found to contain fatty acids with chains of 14 to 20 carbon atoms, mainly palmitic, myristic, oleinic, palmitoleic and linolenic acids. Small differences were found between groups of horses classed by body weight. The experiment confirmed that the composition of horse fat in terms of mono- and polyunsaturated acid content is interesting from a nutritional point of view. Cervical fat contained average 49% of monounsaturated fatty acids and polyunsaturated fatty acids in horses in mass to 550 kg and mass above 550 kg 12.57% and 10.79%, respectively.
PL
Celem badań była ocena składu kwasów tłuszczowych tłuszczu zapasowego koni rzeźnych. Badaniem objęto tłuszcz karkowy i okołojelitowy pobierany od koni różniących się płcią i wagą w ubojni eksportowej. Wydzielony tłuszcz poddawano estryfikacji metodą opisaną przez Peiskera. Rozdział i analizę składu kwasów tłuszczowych przeprowadzono metodą chromatografii gazowej z detektorem płomieniowo-jonizacyjnym. Wykazano występowanie kwasów tłuszczowych o długości łańcucha od 14 do 20 atomów węgla, głównie palmitynowego, mirystynowego, oleinowego, palmitooleinowego i linolenowego. Stwierdzono niewielkie różnice w przypadku koni podzielonych według płci i masy ciała. W badaniach potwierdzono, że tłuszcz koński charakteryzuje się interesującym pod względem wartości żywieniowej składem jedno- i wielonienasyconych kwasów tłuszczowych. W tłuszczu karkowym stwierdzono średnio około 49% jednonienasyconych kwasów tłuszczowych niezależnie od masy ciała koni, natomiast kwasów wielonienasyconych w tłuszczu koni o masie do 550 kg – 12.57%, a u zwierząt o masie powyżej 550 kg – 10,79%,.
EN
This article presents data on the fatty acid composition of avocado fruit and the dynamics of the free fatty acid content during storage of fruit treated with preparations “Agrohit”, “KHAN-8”, and “Extrasol-90”, which have antimicrobial properties. Avocado fruit treated with preparations by spraying and without treatment were placed in polymer containers and stored at a temperature of (4±1)°C and relative humidity of 90–95% for 45 days. During the storage of avocado fruit, the fat content was determined by the Soxhlet method and free fatty acids were determined on a Shimadzu 20-AD chromatograph. It has been established that palmitic, palmitoleic, oleic, and linolenic acids predominate in Fuerto avocado fruit. It has been shown that during storage of avocado fruit treated with preparations, the hydrolysis of fats (triacylglycerides) is slowed down, which reduces the accumulation of free fatty acids. It was determined that treatment of fruit with the preparation “KHAN-8” slows down the oxidation of arachidonic acid, which is a resistance inducer of the fruit to phytopathogens.
EN
Local single cell microalgae isolated from a wastewater swamp and identified as Scenedesmus obliquus was used to determine its applicability for utilization of domestic wastewater for biomass and lipid production. Secondary treated domestic wastewater with or without mixing of growth medium was used to cultivate S. obliquus for the biomass and lipid production as a renewable feedstock for biodiesel. S. obliquus showed the highest OD when grown in 100% Bold’s basal medium (BBM). S. obliquus utilized 95.2% and 78.5% of P and N contents, respectively, when grown in 25% WW+75% BBM mixture and the utilization efficiency of both elements decreased with the increasing wastewater portion in the mixture. Although the BBM displayed the highest dry biomass and lipid production (25.15% of the cell dry biomass). The lowest values were recorded for the uninoculated wastewater, followed by 100% wastewater enriched with S. obliquus. The obtained data revealed that the lipid classes of S. obliquus differs according to the cultivation medium and conditions. The highest percentage of C16-C18 fatty acids (54.76% from total lipids) were recorded in case of algae cultivated in 100% wastewater, followed by 46.96% in case of 100% BBM medium. These results suggest the utilization of mixtures containing a higher portion of secondary treated wastewater, such as 75% WW+25% BBM or 50% WW+50% BBM, could increase the economical production of the lipid-rich microalgae S. obliquus for biodiesel through saving water and nutrients.
EN
Fatty acid (FA) composition of eggs from nine poultry species was determined chromatographically.Twenty six FAs were determined in the lipid composition of eggs. Monounsaturated fatty acids (MUFA) were found in higher amounts than saturated fatty acids (SFA) and polyunsaturated fatty acids (PUFA) in egg yolks. Oleic acid (C18:1 n9) was the major MUFA in egg yolk and, palmitic acid (C16:0) was the major SFA in nine of all different originated eggs. Linoleic acid (C18:2 n6),arachidonic acid (C20:4 n6) and linolenic acid (C18:3 n3), reached the highest levels among the PUFAs. SFA/PUFA ratios were found to be 0.97, 2.51, 2.20, 1.46, 1.67, 1.40, 1.96, 1.27, and 1.34 in chicken, goose, duck, turkey, peacock, guinea fowl, pheasant, quail and partridge, respectively.Eggs of all nine species were found valuable for human consumption as fatty acids source, but the chicken’s egg occured to be the most beneficial to human health according to its highest omega 6 fatty acid (29.8%), highest PUFA / MUFA ratio (0.80) and lowest of SFA / PUFA ratio (0.97).
EN
Polyunsaturated fatty acids (PUFAs, LA and ALA) are not synthesized in mammals in the absence of their essential fatty acid precursors. However, hibernating animals and animals sleeping through the winter need sufficiently high amounts of these acids. The Eurasian badger does not hibernate, but sleeps over winter. In the autumn the body weight of adult badgers increases even twofold, since they put on fat before the winter. Fat is deposited primarily in the subcutaneous layer of adipose tissue, and much less commonly in the muscles, liver and around the intestines. The percentage composition of fatty acids (Saturated fatty acids-SFAs, monounsaturated fatty acids-MUFAs, PUFAs) is different in particular types of tissue. The lipids isolated from depot adipose tissues (suet, subcutaneous, perirenal, periintestinal fat) are dominated by monounsaturated fatty acids (on average 41.25%), followed by saturated fatty acids (33.53%). Polyun­saturated FAs have the lowest proportion in this groups of tissues, on average 17.75% of total FAs. On the other hand, liver lipids contain over 44% PUFAs. The fatty acid composition of lipids in badgers tissue includes considerable quantities of essential unsaturated n-6 and n-3 fatty acids of great pharmacological significance.
EN
The study aimed at determining fatty acid (FA) profiles of meat of seasonally fed slow-growing rabbits in relation to lipid composition of feeds and their intake. In each season, 21 weaned male rabbits (Popielno White breed) were assigned into two homogenous groups: the control (C, n=7) and seasonally fed - summer (S, n=14) or winter (W, n=14). Meat lipid, MUFA and PUFA contents were significantly higher in the S and C groups, most likely due to a higher lipid intake, while S-rabbits had two-fold higher n-3 PUFA contents than C-rabbits. This resulted in a better thrombogenic index in S- rather than in C-rabbits, similarly as it was the case for the hypocholesterolemic/ hypercholesterolemic (H/H) ratio. In relation to the other groups myristic and palmitic acid contents in meat were greater in W-rabbits due to a higher SFA intake. As compared with the C diet, seasonal diets were associated with a higher proportion of n-3 PUFA in meat. This resulted in an improvement of the n-6/n-3 ratio (3.62 on average) in meat from seasonally fed rabbits. Both seasonal diets also increased the content of odd-numbered and branched-chain acids (OBCFA; twofold greater than in the C group). S-rabbits had greater daily body mass gains and were heavier than the W-rabbits. The content of palmitooleic acid (C16:1 n-7) in meat was high irrespective of the diet. Feeding strategies associated with outdoor rearing, adopted in small farms, favour local rabbit populations. Thus, it is recommended to promote native, slow-growing rabbit breeds, a source of valuable nutrients.
EN
Changes in cellular fatty acid composition during naphthalene degradation, at the concentrations of 0.5 g l⁻¹ or 1.0 g l⁻¹, by Pseudomonas sp. JS150 were investigated. In response to naphthalene exposure an increase in saturated/unsaturated ratio was observed. Additionally, the dynamic changes involved alterations in the contents of hydroxy, cyclopropane and branched fatty acids. Among the classes of fatty acids tested the most noticeable changes in the abundance of cyclopropane fatty acids were observed. Since day 4 of incubation these fatty acids were not dectected in bacterial cells growing on naphthalene. In contrast, markedly increased in the percentage of hydroxy fatty acids over time was observed. However, the proportions of saturated straight-chain and branched fatty acids did not change such significantly.
EN
Culled breeding ostriches weighing 130-160 kg yield about 25 kg fat tissue that could possibly be used for supplementing human and animal diet. Cholesterol content of breast fat – 80 mg/100 g tissue – from the breeding ostrich females (n = 6) culled and slaughtered at the age of five years appeared similar to that reported of beef and lamb adipose tissue. In a sum of 17 fatty acids (FA) determined, the mono- and polyunsaturated FA accounted for 37.51 and 38.84%, respectively. The ratio polyunsaturated/saturated FA in breast fat of culled breeding ostrich females appeared higher than reported for other avian species.It is concluded that fat from culled breeding ostriches can be used as an additive to human or animal diet, thus allowing the farmer to gain more from ostrich production. Further research is needed on composition and properties of adipose tissue of ostriches, including slaughter, as well as breeding birds culled at different ages.
PL
Wybrakowane strusie hodowlane o masie ciała 130-160 kg mogą po uboju dostarczać około 25 kg tkanki tłuszczowej.Zawartość cholesterolu, która wyniosła 80 mg/100 g tkanki tłuszczowej sześciu samic hodowlanych,wybrakowanych i ubitych w wieku 5 lat, okazała się podobna do podawanej dla tkanki tłuszczowej wołowiny i baraniny. W sumie 17 oznaczonych kwasów tłuszczowych stwierdzono 37,51% kwasów jedno- i 38,84% kwasów wielonienasyconych. Stosunek kwasów wielonienasyconych do nasyconych był wyższy niż podawany dla tkanki tłuszczowej innych gatunków drobiu. Wnioskuje się o możliwości stosowania tłuszczu młodych strusi hodowlanych, które wybrakowano z przyczyn niezwiązanych z wiekiem, jako dodatku wzbogacającego dietę ludzi i zwierząt. Postępowanie takie mogłoby prowadzić do wzrostu dochodów producentów strusi. Potrzebne są dalsze badania składu i właściwości tkanki tłuszczowej strusi w różnym wieku, włącznie z ptakami rzeźnymi, a także starymi,wybrakowanymi w wieku nawet 15-20 lat.
16
Content available Fatty acid composition of blended spreads
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EN
The paper covers characteristics of fatty acid compositions of blends consisting of milk and vegetable fats. The analyses conducted showed considerable differences in the fatty acid composition and, as well as in the composition and nutritional value of fats belonging to the same product category. The investigated samples contained between 27.1 and 62.2% of saturated fatty acids, while trans isomers of fatty acids were found at a level between 2.4 and 14.8%. Differences in monounsaturated (28.0–45.4%) and polyunsaturated fatty acids (4.3–16.8%) were observed as well.
PL
W pracy dokonano charakterystyki profilu kwasów tłuszczowych mieszanych tłuszczów mleczno- roślinnych. Analiza wykazała znaczne zróżnicowanie wśród badanych, przynależących do tej samej grupy asortymentowej, produktów - zarówno w zakresie profilu kwasów tłuszczowych, jak i wynikającej z tego profilu wartości żywieniowej tłuszczów. Kwasy nasycone występowały w ilości od 27,1% do 62,2%, izomery trans kwasów tłuszczowych w ilości od 2,4% do 14,8%. Badane próby były również znacznie zróżnicowane pod względem zawartości kwasów jednonienasyconych – od 28,0 do 45,4% i wielonienasyconych – od 4,3 do 16,8%.
EN
Lipids obtained from the muscular and adipose tissues of the European beaver were isolated by the modified Folch method. Fatty acids were converted to methyl esters and separated by high-resolution gas chromatography (HR-GC). The content and composition of beaver fat depended on the sex of a given animal. The adipose subcutaneous tissue of the female contained the most fat (approximately 70.5%). The fat content of muscular tissue was very low in both male and female beavers. Adipose tissue lipids of the beaver contained fatty acids ranging in chain length from 12 to 22 carbon atoms. Polyunsaturated fatty acids have the highest proportion in of total FA content of adipose tissue in the beaver, which distinguishes these lipids from the lipids of adipose tissues in other mammals. The results of the present study also confirmed a unique FA composition in the tail fat of the beaver, including a very high content of alpha-linolenic acid (ALA, 18:3 n-3) (on average 20.0%) and the sum of n-3 fatty acids (on average 20.45%). In addition, a very low content of the sum of saturated fatty acids (on average 14.93%) was observed, and an extremely low content, as for animal fat, of palmitic acid 16:0 (on average 10.53%).
EN
Extensive research have indicated that commercial conjugated linoleic acid (CLA), fed to laboratory animals, showed several health-related properties. The objective of this study was to assess the effect of CLA on serum lipid profile, plasma malondialdehyde and liver fatty acids profile in Wistar rats fed for 23 d. The experimental diets were: I-AIN-93G - control (C), II- AIN-93G diet + 1.0% CLA. The CLA oil contained 600 g CLA/kg, with equal representation of cis-9,trans-11 and trans-10,cis-12 CLA isomers. The experimental treatments had no effect on rats body weight, total cholesterol, HDL, LDL+VLDL and malondialdehyde. The triacylglycerol (TG) was significantly decreased after CLA supplementation. Liver weight, fat and histology were unchanged in CLA group. Liver cholesterol was insignificantly decreased in CLA diet. Dietary treatments had significant effects of on proportions of SFA and MUFA and PUFA in liver. In conclusion, CLA decreases serum TG. Additionally, liver fat composition were changed after CLA supplementation
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