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1

Drug resistance profile and detection of genes responsible for methicillin resistance in Staphylococcus aureus isolated from municipal waste

Wolny-Koładka Katarzyna

Journal of Water and Land Development

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2023

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no. 56

136--141

EN

Currently, we are facing the ever-increasing phenomenon of bacteria being resistant to antibiotics. It is the consequence of excessive and incorrect use of drugs. The phenomenon is a global problem affecting bacteria both in their hospital population and in the natural environment. Municipal waste is an environment conducive to the development of microorganisms, therefore it contains various groups of bacteria, including drug-resistant staphylococci. The aim of the study was to identify species of bacteria, determine their antibiotic resistance, and assess the occurrence of genes responsible for methicillin resistance in Staphylococcus aureus isolated from mixed municipal waste. Strains were isolated by Koch’s serial dilution method with the use of microbiological media. Species were identified using the MALDI TOF-MS technique, whereas the drug resistance profile was determined by disk diffusion and molecular PCR methods. 250 isolates of S. aureus were collected. The highest resistance found was to cefoxitin, erythromycin and tetracycline. Among the bacteria collected, resistance to 1, 2, 3 or 4 antibiotics at the same time was the most common, with a maximum of 10. Additionally, 45 (18%) MDR (multidrug-resistant) isolates were detected. Methicillin resistance was found by the disk diffusion test in 60 (24%) strains, while the mecA gene was detected in as many as 180 (72%) isolates.

2

Eco friendly synthesis of silver oxide nanoparticles from borassus flabellifer fiber and its antibacterial activity against representative micro organisms

Vanitha R., Kavitha C., Ananda Kumar S.

Advances in Materials Science

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2023

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Vol. 23, nr 4(78)

27--36

EN

The present study reports an easy eco-friendly, cost efficient, and rapid method for the synthesis of silver nanoparticles (Ag NPs) using palm sprouts as reducing cum capping agent. Green synthesis of silver nanoparticles was successfully performed using palm sprouts plant extract via a simple and cheaper eco-friendly method. Palm sprouts extract reduces silver nitrate to silver nanoparticles. The resulting materials were analyzed by Fourier-transform infrared spectroscopy (FTIR), Scanning Electron Microscopy (SEM), and X-ray Diffraction (XRD) analysis. FT-IR spectrum confirms the presence of various functional groups in the active biomolecules, it acts as a capping agent for the nanoparticles. The morphology of this sample was analyzed through SEM and the presence of silver was confirmed accordingly. The green synthesized Ag NPs exhibited an excellent antibacterial activity against E. coli and P. aeruginosa and B. subtilis and S. aureus besides imparting efficient antimicrobial activity against pathogenic bacteria as well.

3

Aminokwasy, glikany, peptydy i białka w ścieżkach diagnostycznych i terapeutycznych chorób cywilizacyjnych XXI wieku : projektowanie i charakterystyka fizykochemiczna oraz strukturalna

Bylińska Irena, Dzierżyńska Maria, Giżyńska Małgorzata, Guzow Katarzyna, Jankowska Elżbieta, Jurczak Przemysław, Kaczyński Zbigniew, Karska Natalia, Kowalczyk Agnieszka, Kuncewicz Katarzyna, Orlikowska Marta, Sawicka Justyna, Spodzieja Marta, Szpakowska Nikola, Szymańska Aneta, Wieczerzak Ewa, Witkowska Julia, Rodziewicz-Motowidło Sylwia

Wiadomości Chemiczne

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2022

|

[Z] 76, 5-6

393--431

EN

The civilization diseases of the 21st century are non-infectious disorders, affecting a large part of modern society. They are associated with the significant development of industry and technology, and hence with environmental pollution and an unhealthy lifestyle. These factors have led to the development of many civilization diseases, which currently include: cardiovascular diseases, respiratory diseases, diabetes, obesity, malignant tumors, gastrointestinal diseases, mental disorders and allergic diseases. The development of technologies, including modern therapies and new drugs, resulted in increase in life expectancy. This creates a global problem of an aging population with an increasing number of diseases of the old age, i.e. dementias. In addition, sedentary lifestyles and changing diets are the reasons why more and more people develop metabolic diseases, as well as neurological and cognitive disorders characterized by progressive damage to nerve cells and dementia. Currently, problem on a global scale is also the growing resistance to existing antimicrobial drugs. Therefore, the scientists face many challenges related to searching for the causes of these diseases, their diagnosis and treatment. Scientific research conducted at the Department of Biomedical Chemistry at the Faculty of Chemistry of the University of Gdańsk is part of this research trend. In this publication, we discuss various research topics with the long-term aim of solving the problems associated with the diseases mentioned above. The following chapters are dedicated to (i) looking for new effective fluorophores with diagnostic and anti-cancer activity; (ii) designing of new compounds with antibacterial and antiviral activity and their synthesis; (iii) investigating the mechanisms of amyloid deposit formation by human cystatin C and possibilities of inhibition of this process; (iv) designing and studies of compounds activating the proteasome with the potential to suppress the development of neurodegenerative diseases; (v) designing peptide fibrils and hydrogels as drug carriers; (vi) searching for peptide inhibitors of immune checkpoint as potential drugs for immunotherapy; (vii) studying the mechanism of action of selected herpesviruses by determining the structure of viral proteins and (viii) studying the composition of natural glycans and glycoconjugates in order to better understand the mechanisms of interaction of bacteria with the environment or with the host.

4

Staphylococcus aureus - patogen przemysłowy

Sztucki Damian

Laboratorium - Przegląd Ogólnopolski

|

2021

|

nr 4

31--35

PL

Staphylococcus aureus jest czynnikiem wywołującym wiele chorób zakaźnych, takich jak infekcje skóry, bakteriemia, zapalenie wsierdzia, zapalenie płuc i zatrucia pokarmowe. Organizm ten był pierwotnie wiodącym patogenem szpitalnym, a następnie w środowisku społecznym pojawiły się odrębne epidemiologicznie klony.

5

Silver-clay nanohybrid as a coating for improvement of the antibacterial properties of paper

Alikhah Tahereh, Babavand Arash, Afra Elyas, Ghasemian Ali, Saraeian Ahmadreza

Drewno : prace naukowe, doniesienia, komunikaty

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2020

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vol. 63, nr 205

109--124

EN

This comparative study was carried out to investigate the effect of nanosilver, silver-clay nanohybrid, and silver-milled clay nanohybrid coatings on the antibacterial characteristics of paper. Nanosilver (25 ppm) was used as a singleand double-layer coating. The results demonstrated that treatment with pure nanosilver was more efficient in the reduction of microbial growth. Also, milling of clay enabled better maintenance of silver nanoparticles, and led to a greater decrease in bacterial growth than in the case of the original silver-clay nanohybrid. Evaluation of the sustainability of antibacterial characteristics confirmed that, although pure nanosilver treatment achieved better performance in the first 15 minutes than nanohybrid samples, the performance of the nanohybrids improved with the passing of time. As expected, the treatments decreased the brightness of paper, while the opacity increased significantly; pure nanosilver treatment led to lower brightness than the others, and the opacity was higher in the case of the silver-clay nanohybrid than with the other treatments.

6

Występowanie Staphylococcus aureus w krewetkach pochodzących z rynku

Kukułowicz A.

Zeszyty Naukowe Akademii Morskiej w Gdyni

|

2016

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nr 93

59--63

PL

Artykuł omawia spożywanie skażonych produktów spożywczych jako jedną z głównych przyczyn chorób przenoszonych drogą pokarmową. Celem niniejszych badań była ocena bezpieczeństwa krewetek świeżych, mrożonych i pochodzących z zalewy ze względu na występowanie koagulazododatnich Staphylococcus aureus. Największy odsetek prób (około 33%), w których nie obserwowano wzrostu komórek Staphylococcus aureus, pochodził z obranych krewetek mrożonych oraz krewetek w zalewie z chlorkiem sodu.

EN

Food safety is of high importance the protection of public health is concerned. Despite the continuous progress in the development of knowledge and techniques in the field of food security, consumption of contaminated food products still represents one of the major causes of food-borne diseases. The purpose of this study was to assess the safety of fresh, frozen and pickled shrimps due to the presence of coagulase-positive Staphylococcus aureus. The largest proportion (approximately 33%), where the growth of Staphylococcus aureus cells was not observed came from the frozen shrimps and prawns/ shrimps in brine/pickle with sodium chloride.

7

Identyfikacja antybiotykoopornych Staphylococcus aureus w zupach liofilizowanych

Steinka I.

Zeszyty Naukowe Akademii Morskiej w Gdyni

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2015

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nr 88

12--16

PL

Antybiotykooporność bakterii izolowanych z żywności stanowi problem higieniczny. Specyficzne właściwości gronkowców pozwalają na przetrwanie warunków liofilizacji w procesie produkcji, co stanowi powód obawy o możliwość przeżywania antybiotykoopornych Staphylococcus aureus. W zupach liofilizowanych obecnych na polskim rynku stwierdzono obecność gronkowców metycylinoopornych w 13,3% próbek badanych, należących do dwóch producentów: Mammita i Knorr. Składnikami zup, z których izolowano gronkowce antybiotykooporne, były: ser, makaron szpinakowy i rosół z kurczaka. Prawdopodobnym źródłem MRSA są składniki stosowane w technologii wytwarzania zup.

EN

Antibiotic-resistant bacteria isolated from food is a hygiene problem. The specific properties of staphylococci allow them to survive the freeze-drying conditions in the production process. It is a reason for concern about the possible survival of antibiotic-resistant Staphylococcus aureus. The freeze-dried soups on the Polish market revealed the presence of methicillin-resistant staphylococci in 13,3% of samples tested belonging to two manufacturers: Mammita and Knorr. The ingredients in soup, all of which are antibiotic-resistant staphylococci isolated cheese, spinach pasta and chicken soup. A possible source of MRSA are ingredients used in formulation technology soups.

8

Immunomodulacyjne właściwości mikroorganizmów

Ograczyk E., Micota B., Miszczyk E., Gajewski E., Włodarczyk M., Rudnicka K., Sadowska B.

LAB Laboratoria, Aparatura, Badania

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2015

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R. 20, nr 3

11--18

9

Gentamicin loaded PLGA nanoparticles as local drug delivery system for the osteomyelitis treatment

Posadowska U., Brzychczy-Włoch M., Pamuła E.

Acta of Bioengineering and Biomechanics

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2015

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Vol. 17, no. 3

41--48

EN

Since there are more and more cases of multiresistance among microorganisms, rational use of antibiotics (especially their systemic vs. local application) is of great importance. Here we propose polymeric nanoparticles as locally applied gentamicin delivery system useful in osteomyelitis therapy. Gentamicin sulphate (GS) was encapsulated in the poly(lactide-co-glycolide) (PLGA 85:15) nanoparticles by double emulsification (water/oil/water, W1/O/W2). The nanoparticles were characterized by dynamic light scattering, laser electrophoresis and atomic force microscopy. UV-vis spectroscopy (O-phthaldialdehyde assay, OPA) and Kirby-Bauer tests were used to evaluate drug release and antimicrobial activity, respectively. Physicochemical characterization showed that size, shape and drug solubilization of the nanoparticles mainly depended on GS content and concentration of surface stabilizer (polyvinyl alcohol, PVA). Laser electrophoresis demonstrated negative value of zeta potential of the nanoparticles attributed to PLGA carboxyl end group presence. Drug release studies showed initial burst release followed by prolonged 35-day sustained gentamicin delivery. Agar-diffusion tests performed with pathogens causing osteomyelitis (Staphylococcus aureus and Staphylococcus epidermidis, both reference strains and clinical isolates) showed antibacterial activity of GS loaded nanoparticles (GS-NPs). It can be concluded that GS-NPs are a promising form of biomaterials useful in osteomyelitis therapy.

10

The costs arising from the contamination of the food by Staphylococcus aureus and production of aloe tissue as anti-staphylococcal addition to fermented dairy products

Kukułowicz A.

Zeszyty Naukowe Akademii Morskiej w Gdyni

|

2014

|

nr 84

66--72

EN

The development of new technologies allows the emergence of new food ingredients, which may have an impact on the nutritional value of the products and safety of consumers health. Thanks to its antibacterial properties, Aloe Vera can effectively improve the microbiological quality of fermented milk products.

11

Surface properties and antimicrobial activity of composite nanofibers of polycaprolactone with silver precipitations

Dobrzański L., Hudecki A., Chladek G., Król W., Mertas A.

Archives of Materials Science and Engineering

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2014

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Vol. 70, nr 2

53--60

EN

Purpose: The purpose of the article is to investigate the structure and antimicrobial properties of composite nanofibers with silver particles precipitated onto the nanofibers surface. Design/methodology/approach: A solution was prepared in the first place made of the following solvents to fabricate antimicrobial composite nanofibers of polycaprolactone with silver precipitations: formic acid and acetic acid at a rate of 70:30. Then, silver nitrate was introduced into the fabricated solution of the solvents and it was subjected to the interaction of ultrasounds, and after 10 minutes polycaprolactone was added to the solution, and then the solution was mixed for 12 hours and a solution was obtained with a 10% concentration and the mass fraction of 0, 1, 3 and 5% of silver nitrate additives. The solution was forced into a positive voltage electrode placed above a negative voltage electrode; the solution was then subjected to the activity of a strong electrostatic field transforming the solution into micro- and nanofibers. After electrospinning, the fibers obtained underwent the activity of a 2% ascorbic acid solution, by means of which silver was precipitated on the nanofibers surface. Viscosity and electrical conductivity tests were performed of single-component and double-component solutions, of the fibers’ structure in a transmission electron microscope, of the BET, Langmuir specific surface area and DTF porosity with the method of gas adsorption and antimicrobial activity of the nanocomposites produced on the nanocomposites on following bacteria: Staphylococcus aureus, Escherichia coli, Candida albicans. Findings: The use of a formic acid and acetic acid solution at a rate of 70:30 for preparation of a polycaprolactone solution for its electrospinning enables to obtain a non-toxic and fully biodegradable polymer nanofibers of polycaprolactone with PCL/Ag silver precipitations possessing high antimicrobial performance against Gram+, Gram- bacteria and fungi. Practical implications: Antimicrobial composite nanofibers with silver nanoparticles precipitated onto the nanofibers surface can be applied in biodegradable antiseptic dressings in the form of mats or other textiles containing polymer nanofibers of polycaprolactone with PCL/Ag silver precipitations obtained as a result of electrospinning. Originality/value: The research outcomes confirm that it is feasible to manufacture polycaprolactone nanofibers with PCL/Ag silver precipitations possessing high antimicrobial performance against Gram+, Gram- bacteria and fungi.

12

Wpływ fosfatydylobetulin na bakterie Staphylococcus aureus

Malicki A., Tubek-Barycza B., Janik P., Szpak M., Źródłowska-Danek J., Grabowski T., Szyplik K., Wawrzeńczyk C.

Przemysł Chemiczny

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2013

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T. 92, nr 8

1532-1537

13

Wpływ wilgotności względnej powietrza na przeżywalność bakterii w poliestrowych włókninach filtracyjnych

Krakowiak P., Żakowska Z.

Przegląd Włókienniczy - Włókno, Odzież, Skóra

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2013

|

nr 3

29-32

EN

The study deals with characterization of microorganism's dynamic growth in different relative humidity such as Bacillus subtilis, Micrococcus flavus, Staphylococcus aureus, Escherichia coli in fiber filters media used In air purifications systems. Viability of airborne microorganisms was different depending on RH condition. Highest viability, even when RH value was 20%, characterized Bacillus subtilis - spore forming bacteria. Low survival was indicated by bacteria Escherichia coli and Staphylococcus aureus.

14

Antibacterial dyeing of polyamide using turmeric as a natural dye

Mirjalili M., Karimi L

Autex Research Journal

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2013

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Vol. 13, no. 2

51--56

EN

Curcuma longa rhizome (turmeric) is a medicinal plant used for fabric and food coloration. In this study, polyamide (nylon 6.6) fabric was dyed with different mordants at various turmeric concentrations. The dyed fabric was evaluated for bacteriostatic activity against pathogenic strains of Gram-positive (Staphylococcus aureus) and Gramnegative (Escherichia coli) bacteria. The relationship between bacteriostatic activity and turmeric concentration was investigated. Durability of antibacterial activity to laundering is also discussed. Results indicate that the polyamide dyed with turmeric displayed excellent antibacterial activity in the presence of ferric sulfate, cupric sulfate, and potassium aluminum sulfate, and exhibited good and durable fastness properties.

15

Przeżywalność bakterii Staphylococcus aureus na tkaninach użytkowych

Jekiel K., Szostak-Kot J., Syguła-Cholewińska J.

Przegląd Włókienniczy - Włókno, Odzież, Skóra

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2011

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nr 11-12

32-35

16

Evaluation of Berberine Chloride as a New Antibacterial Agent Against Gram-Positive Bacteria for Medical Textiles

Sarkar A K, Appidi S, Ranganath A S

Fibres & Textiles in Eastern Europe

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2011

|

Vol. 19, no. 4 (87)

131--134

EN

Berberine, an isoquinoline alkaloid, is reported to possess excellent antibacterial properties. Berberine is found in the roots of Coptis chinensis and the stems of phellodendron. In the current study, the efficacy of berberine chloride as a new antibacterial agent on textile substrates against Staphylococcus aureus and Enterococcus faecalis was investigated. In particular, the relationship between bacterial inhibition and the concentration of berberine chloride needed to exhibit effective bacterial action was studied. Results showed berberine chloride to be an effective antibacterial agent at a minimum inhibitory concentration of 0.2% in the solution. On textile substrates such as 100% polyester, 100% nylon and 50% cotton-50% polyester blend, there was a 62-76% reduction in bacterial counts. The effectiveness of antibacterial action was retained after laundering and exposure to light.

PL

Berberyna, izochinolinowy alkaloid, posiada świetne właściwości antybakteryjne. Berberynę uzyskuje się z korzeni berberysu chińskiego i łodyg filodendronu. W pracy badano efektywność chlorku berberyny jako środka antybakteryjnego stosowanego w tekstylnych wyrobach medycznych. W szczególności zbadano zależność pomiędzy stężeniem chlorku berberyny a stopniem zahamowania wzrostu bakterii. Stwierdzono, że chlorek berberyny jest świetnym środkiem antybakteryjnym nawet przy małych stężeniach ( 0.2% ) w roztworze. Dla próbek wykonanych z 100% poliestru, 100% poliamidu i mieszanki 50%/50% bawełna/poliester uzyskano zmniejszenie liczby bakterii o 62% -76%. Próbki wykazywały właściwości antybakteryjne również po procesie prania i poddaniu działaniu światła.

17

Sery dojrzewające jako środowisko rozwoju patogenów

Berthold A., Pluta A., Dolińska M.

Postępy Techniki Przetwórstwa Spożywczego

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2008

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nr 1

79-84

PL

Celem artykułu jest omówienie możliwości rozwoju w serach podpuszczkowych dojrzewających drobnoustrojów patogennych, takich jak: Listeria monocytogenes, Escherichia coli. Salmonella sp. oraz Staphylococcus aureus. Przeanalizowano wpływ różnych czynników technologicznych wpływających na rozwój patogenów w serach. Dokonano przeglądu piśmiennictwa dotyczącego jakości mikrobiologicznej serów rynkowych. Przedstawiono przypadki zatruć pokarmowych wywołanych spożyciem serów dojrzęwająacych, zanieczyszczonych drobnoustrojami chorobotwórczymi.

EN

The aim of the study was to review the possibilities of growth in rennet cheeses pathogenic microorganisms, such as: Listeria monocytogenes, Escherichia coli, Salmonella sp. and Staphylococcus aureus. The influence of some factors on the growth of pathogenic bacteria was analysed. The review of the literature concerning the microbiological quality of market cheeses was made. The cases of food pisonings, caused by consumption of rennet cheeses, contaminated with pathogenic bacteria were shown.

18

Staphylococcus aureus w żywności - charakterystyka, detekcja, zwalczanie

Satora P.

Laboratorium - Przegląd Ogólnopolski

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2008

|

nr 9

36--41

PL

Staphylococcus aureus jest gram-dodatnią bakterią zdolną do wytwarzania szeregu enterotoksyn, które spożyte powodują intoksykację. Artykuł poświęcony jest ogólnej charakterystyce bakterii z gatunku Staphylococcus aureus i jej enterotoksyn, a także metodom wykrywania tego mikroorganizmu w żywności. Omówione zostały również produkty szczególnie narażone na zanieczyszczenie tym drobnoustrojem i sposoby kontroli bakterii w żywności.

EN

Staphylococcus aureus is a gram-positive bacterium able to produce 12 different enterotoxins which, after consumption, cause staphylococcal intoxication. The article includes a general characteristics of the Staphylococcus aureus species, with its enterotoxins, as well as methods of detection of this microorganism in food. Food products which are particularly involved in staphylococcal contamination and methods of control in food are also discussed.

19

Antybakteryjne działanie żelu bogatopłytkowego - badania in vitro

Bielecki T., Gaździk T.S., Ziółkowski G., Pomorska-Wesołowska M.

Inżynieria Biomateriałów

|

2006

|

R. 9, nr 54-55

46-49

PL

Mnogość substancji znajdująca się w płytkach, które biorą udział w procesie krzepnięcia, gojenia tkanek i odpowiedzi immunologicznej świadczy o wielkiej roli tych elementów morfologicznych krwi w fizjologii i patologii człowieka. W dostępnej literaturze nie ma publikacji na temat biomateriałów łączących zdolność pobudzenia procesów gojenia z właściwościami antybakteryjnymi. Celem niniejszej pracy było wykazanie antybakteryjnych właściwości żelu bogatopłytkowego. Po okresie inkubacji odczytano wyniki. Średni obszar zahamowania wzrostu w zależności od rodzaju bakterii wahał się między 6,0 a 20,8 mm. Zastosowanie wyżej wymienionego biomateriału, który posiada właściwości bakteriostatyczne i bakteriobójcze może stać się przełomem w leczeniu infekcji tkanek miękkich i ran pourazowych nie tylko w ortopedii, ale również na oddziałach chirurgii ogólnej, naczyniowej, szczękowo-twarzowej, plastycznej oraz torakochirurgii i neurochirurgii

EN

A lot of biological substances which are contained in platelets take a part in coagulation process, tissues healing and in immunological answer, witnesses about huge role these blood elements in physiology and pathology. In literature there are no publications about biomaterials which are able to stimulate healing process and which have antibacterial features. The aim of this experiment is to examine antimicrobial effects of platelet gel. During incubation period results were read. Average area of growth restrain in dependence on bacteria kind between 6,0 and 20,8 mm. Using this biomaterial which own bactericidal and bacteriostatic properties can become a breakthrough in treatment tissue infections and posttraumatic wounds not only in orthopaedics but also surgery vascular, thoracic surgery maxillofacial surgery, plastics surgery and neurosurgery words

20

Gdyby Shaw i Fleming żyli dzisiaj...

Ochocka B.

Laboratorium - Przegląd Ogólnopolski

|

2002

|

nr 4

16

PL

Wśród gronkowców złocistych coraz większy odsetek stanowią szczepy odporne na metycylinę - MRSA. Pojęcie oporności na metycylinę zawiera w sobie informację, że bakteria ta jest oporna na lecznie większością znanych antybiotyków. Badania wykazują, że MRSA ma także coraz większy udział w tzw. zakażeniach pozaszpitalnych oraz że bakteria ta bardzo szybko rozprzstrzenia się w ekosystemie szpitali. Wysoki udział szczepów MRSA (od 12,5% do 50%) stawia Polskę w czołówce państw o wysokim udziale MRSA w zakażeniach szpitalnych.