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Opracowanie metody oznaczania iwermektyny w jadalnych tkankach zwierzat rzeznych

100%

Oledzka I. , Kowalski P.

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tom 38

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nr Supl.

387-390

PL

Opracowano metodą oznaczania iwermektyny w surowicy i tkankach jadalnych zwierząt z zastosowaniem elektroforezy kapilarnej, którą poddano ocenie statystycznej. Proces walidacji uwzględniał następujące elementy: liniowość w zakresie od 1 do 20 µg/kg, czułość, specyficzność, dokładność i precyzję metody. Odzysk analitu w procesie ekstrakcji wynosił 80,2%. Granicę wykrywalności ustalono na 0,3 µg/kg a oznaczalności ilościowej na 1 µg/kg.

EN

In this work the capillary clectrophoretic method, with liquid-liquid extraction, for the isolation and determination of the ivermectin in animal serum and tissues is presented. Ivermectin is a drug with a broad spectrum of activity against nematodes and arthropod parasites in food-producting animals such as sheep, cattle, pigs, horses. The method was statistically validated for its linearity, accurancy, precision and selectivity. A typical standard curve encompassing a range of 1 to 20 µg/kg ivermectin was linear (correlation coefficient r=0.9992). The lowest level validated in scrum and tissue by the method was 1 µg/kg and the limit of detection was 0.3 µg/kg. The average recovery from samples was 80.72%. The inter-assay precision was expressed as the mean SD and relative standard deviation (RSD). This characteristics make this method attractive when compared to classical isolation and determination procedures for ivermectin. The analytical method was successfully applied to determination for ivermectin residue in tissues and for pharmacokinetic studies.

2

Pozostalosci kokcydiostatykow w tkankach jadalnych drobiu

100%

Kowalski P. , Oledzka I.

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tom 38

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nr Supl.

391-394

PL

Opracowano metodą elektroforetycznego oznaczania pozostałości nifursolu w tkankach jadalnych indyków (mięśnie, skóra z tłuszczem, wątroba i nerki). Przeprowadzono walidacją całej procedury oznaczania nifursolu; limit wykrywalności ustalono na 7 g/kg w badanych tkankach. Przedstawiona metoda jest specyficzna, precyzyjna, czuła i wystarczająco dokładna do oznaczania pozostałości leków weterynaryjnych w tkankach pochodzenia zwierzęcego.

EN

The use of veterinary drugs (antibiotics, coccidiostatics) to treat animal disease and to promote fast, more efficient growth of livestock is a common practice in modern agriculture. In order to protect the health of the consumer of foodstuffs of animal origin, one of the most important principles laid down in the legislation is that foodstuffs obtained from animals treated with veterinary medicinal products must not contain residues of the medicine or its metabolites which might constitute a health hazard for the consumer. One of the most common anticoccidial drug used in EU to 2003 was nifursol. The aim of this study was to develop a confirmatory analysis by capillary electrophoresis (CE) for nifursol residues in edible tissues (muscle, skin with fat, liver and kidney) of turkey origin. The whole of nifursol determination procedure was validated. The described method is specific, sensitive, precise and accurate enough for determination residue of veterinary drugs in tissue of animal origin and could be used for therapeutic drug monitoring.

3

Wykrywanie i oznaczanie pozostalosci florfenikolu i tiamfenikolu w tkankach jadalnych zwierzat metoda elektroforezy kapilarnej

80%

Kowalski P. , Plenis A. , Oledzka I.

Bromatologia i Chemia Toksykologiczna

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2009

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tom 42

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nr 3

569-573

4

Elektroforetyczne oznaczanie kationów: sodu, potasu, wapnia i magnezu w butelkowanych wodach mineralnych

80%

Kowalski P. , Oledzka I. , Plenis A. , Baczek T.

Bromatologia i Chemia Toksykologiczna

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2011

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tom 44

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nr 3

5

Thermal sterilization affects the content of selected compounds in diets for laboratory animals

51%

Tusnio A. , Taciak M. , Barszcz M. , Paradziej-Łukowicz J. , Oledzka I. , Wiczkowski W. , Szumska M. , Pastuszewska B. , Skomial J.

Journal of Animal and Feed Sciences

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2014

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tom 23

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nr 4

EN

Autoclaving is the current method of choice for sterilization of diets for specified-pathogen-free (SPF) laboratory animals, but this process may negatively affect the nutrient content (e.g., protein, vitamin) of the diets. Two natural-ingredient diets supplemented with either soyabean meal containing phytoestrogens (S) or with casein (C), and a commercial autoclavable (SN) diet, were autoclaved at 121°C for 20 min (T1) or at 134°C for 10 min (T2). Chemical composition, selected water- and fat-soluble vitamins, and acrylamide were analysed in all non-autoclaved (T0) and autoclaved diets, whereas in S diets, oestrogenic isoflavones were also determined. Autoclaving affected most protein bound to NDF macronutrient contents only to a small degree, except those of NDF and N-NDF. The contents of NDF and N-NDF were increased by T2 and T1; the change was the largest in the SN and the smallest in the C diet. Losses of particular vitamins differed among the diets and treatments. Among the water-soluble vitamins, thiamine, riboflavin and pyridoxine were the least affected, whereas calcium pantothenate was lowered by T1 and T2. Among fat-soluble vitamins, vitamin E was the most stable, while vitamins A and D, the least stable, but even the highest vitamin losses did not exceed 50%. The acrylamide concentration increased more in diets autoclaved at T2 than at T1 and in the S and C diets than in SN. Autoclaving the S diet increased the daidzin and genistin contents and slightly reduced the total isoflavone content. It is concluded that the effects of autoclaving different diets are not uniform, but longer autoclaving at a lower temperature (T1) is less detrimental than shorter treatment at a higher temperature (T2), mainly because of the smaller increase in the NDF and N-NDF contents and acrylamide concentration.