Wyniki wyszukiwania - Biblioteka Nauki

1

Purification and functional reconstitution of intact ral-binding GTPase activating protein, RLIP76, in artificial liposomes.

100%

Singhal S. , Singhal J. , Cheng J. , Pikuła S. , Sharma R. , Zimniak P. , Awasthi Y. , Awasthi S.

Acta Biochimica Polonica

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2001

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tom 48

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nr 2

551-562

EN

We have recently shown that RLIP76, a ral-binding GTPase activating protein, mediates ATP-dependent transport of glutathione-conjugates (GS-E) and doxorubicin (DOX) (S. Awasthi et al., Biochemistry 39, 9327, 2000). Transport function of RLIP76 was found to be intact despite considerable proteolytic fragmentation in preparations used for those studies, suggesting either that the residual intact RLIP76 was responsible for transport activity, or that the transport activity could be reconstituted by fragments of RLIP76. If the former were true, intact RLIP76 would have a much higher specific activity for ATP-hydrolysis than the fragmented protein. We have addressed this question by comparing transport properties of recombinant RLIP76 and human erythrocyte membrane RLIP76 purified in buffers treated with either 100 or 500 μM serine protease inhibitor, PMSF. The purity and identity of recombinant and human erythrocyte RLIP76 was established by SDS/PAGE and Western-blot analysis. These studies confirmed the origin of the 38 kDa protein, previously referred to as DNP-SG ATPase, from RLIP76. Higher PMSF concentration resulted in lower yield of the 38 kDa band and higher yield of intact RLIP76 from both human and recombinant source. In contrast, the substrate-stimulated ATPase activity in presence of DNP-SG, doxorubicin, daunorubicin, or colchicine were unaffected by increased PMSF; similarly, ATP-dependent transport of doxorubicin in proteoliposomes reconstituted with RLIP76 was unaffected by higher PMSF. These results indicated that limited proteolysis by serine proteases does not abrogate the transport function of RLIP76. Comparison of transport kinetics for daunorubicin between recombinant vs human erythrocyte RLIP76 revealed higher specific activity of transport for tissue purified RLIP76, indicating that additional factors present in tissue purified RLIP76 can modulate its transport activity.

2

Granulocyte proteinases as mediators of unspecific proteolysis in inflammation: a review

100%

Fritz H. , Jochum M. , Geiger R. , Duswald K. H. , Dittmer H. , Kortmann S. , Neumann S. , Lang H.

Folia Histochemica et Cytobiologica

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1986

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tom 24

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nr 2

3

On the spatial organization of ubiquitin-dependent proteolysis in HeLa cells

100%

Wojcik C.

Folia Histochemica et Cytobiologica

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1997

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tom 35

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nr 2

4

Antioxidant activity of the peptide fraction from Parmigiano-Reggiano cheeses at different ageing times

86%

Bottesini C. , Paolella S. , Lambertini F. , Galaverna G. , Dossena A. , Marchelli R. , Sforza S.

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tom 61

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nr Suppl.1

5

Susceptibility of yeasts isolated from dairy products to cystatin

86%

Chrzanowska J. , Krajewska E. , Saleh J. , Szoltysik M. , Trziszka T.

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tom 02

23-29

6

Modes of inhibition of cysteine proteases.

86%

Rzychon M. , Chmiel D. , Stec-Niemczyk J.

Acta Biochimica Polonica

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2004

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tom 51

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nr 4

861-873

EN

Cysteine proteases are involved in many physiological processes and their hyperactivity may lead to severe diseases. Nature has developed various strategies to protect cells and whole organisms against undesired proteolysis. One of them is the control of proteolytic activity by inhibition. This paper presents the mechanisms underlying the action of proteinaceous inhibitors of cysteine proteinases and covers propeptides binding backwards relative to the substrate or distorting the protease catalytic centre similarly to serpins, the p35 protein binding covalently to the enzyme, and cystatins that are exosite binding inhibitors. The paper also discusses tyropins and chagasins that, although unrelated to cystatins, inhibit cysteine proteinases by a similar mechanism, as well as inhibitors of the apoptosis protein family that bind in a direction opposite to that of the substrate, similarly to profragments. Special attention is given to staphostatins, a novel family of inhibitors acting in an unusual manner.

7

Biochemical and microbiological changes in cheese inoculated with Yarrowia lipolytica yeast

86%

Szoltysik M. , Dabrowska A. , Babij K. , Pokora M. , Zambrowicz A. , Polomska X. , Wojtatowicz M. , Chrzanowska J.

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2013

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tom 20

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nr 4

EN

A Yarrowia lipolytica JII1c yeast strain, isolated from the Polish ‘Rokpol’ mould cheese, was used as an adjunct culture in the production of a Dutch-type cheese. Its effect on the microbiological and biochemical characteristics of the cheese was evaluated in this research study. Milk used to produce the cheese was inoculated with 105 cfu/mL yeast cells. During the ripening process, the yeast population grew systematically to reach a maximum level of 7.9 log cfu/g in the sixth week of maturation, whereas the number of lactic acid bacteria increased until the fourth week of ripening. Thereafter, the number of microorganisms in the both groups decreased. After 8 weeks of ripening, the pH value of cheese inoculated with yeasts was significantly higher than that of the control cheese sample (produced without those microorganisms) and reached the levels of 6.37 and 5.47, respectively. In the experimental cheeses, it was also found that the utilization rate of lactic and citric acids was higher. Additionally, the concentration levels of water-soluble nitrogen (WSN) and free amino groups (FAG) in the experimental cheeses were about twice as high as in the control cheese sample. A more intensive proteolysis in the experimental cheese was accompanied by a higher accumulation of biogenic amines, especially of tyramine, putrescine, and 2-phenylethylamine; in the experimental cheese, after 8 weeks, their contents amounted to: 167.01, 77.90, and 69.54 mg/100 g, respectively. In contrast, the concentration of histamine was similar in both cheeses (9.47 and 9.81 mg/100 g in the control and experimental cheese samples, respectively). Also, the experimental cheese revealed more pronounced lipolysis resulting in a higher accumulation of free fatty acids, especially of butyric, myristic, palmitic, stearic, and oleic acids. It can be concluded that the Y. lipolytica JII1c grew well in the cheese causing the ripening process of the cheese to significantly accelerate.

PL

Szczep drożdży Yarrowia lipolytica JII1c wyizolowany z sera Rokpol z przerostem pleśni zastosowany został jako kultura wspomagająca do produkcji sera typu holenderskiego. W pracy oceniano jego wpływ na właściwości biochemiczne wyprodukowanego sera oraz na rozwój mikroflory. Mleko użyte do produkcji sera zostało zaszczepione komórkami drożdży w ilości 105 jtk/mL. Podczas dojrzewania liczba drożdży systematycznie wzrastała, osiągając w szóstym tygodniu dojrzewania poziom maksymalny 7,9 log jtk/g, natomiast liczba bakterii mlekowych wzrastała jedynie do czwartego tygodnia dojrzewania. W kolejnych tygodniach dojrzewania liczba obu grup drobnoustrojów stopniowo malała. Po 8 tygodniach dojrzewania pH sera, do którego wprowadzono drożdże, było znacząco wyższe w porównaniu z serami kontrolnymi wyprodukowanymi bez udziału tych mikroorganizmów i osiągnęło wartość odpowiednio 6,37 i 5,47. W doświadczalnych serach obserwowano także intensywniejsze przemiany kwasu mlekowego i cytrynowego, a oznaczone wartości azotu rozpuszczalnego (WSN) i wolnych grup aminowych (FAG) były około 2-krotnie wyższe. Intensywniejszej proteolizie w serach otrzymanych przy udziale drożdży Yarrowia lipolytica towarzyszyła także wyższa zawartość amin biogennych, zwłaszcza tyraminy, putrescyny i 2-fenyloetylaminy. Ich ilości, w przeliczeniu na 100 g sera, wynosiły w 8. tygodniu dojrzewania odpowiednio 167,01, 77,90 i 69,54 mg. Stężenie histaminy w serach badanych i kontrolnych było zbliżone i wynosiło 9,81 i 9,47 mg w 100 g sera. Intensywniejszą lipolizę odnotowano w serach eksperymentalnych, co spowodowało większe stężenie wolnych kwasów tłuszczowych, zwłaszcza masłowego, mirystynowego, palmitynowego, stearynowego i oleinowego. Wykazano, że szczep drożdży Y. lipolytica JII1c wprowadzony do sera wpływał na przyspieszenie procesu jego dojrzewania.

8

Control of postharvest longevity of cut leaves of Nephrolepis exaltata (L.) Schott.

86%

Skutnik E. , Rabiza-Swider J.

Annals of Warsaw Agricultural University. Horticulture and Landscape Architecture

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2005

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tom 26

9

The lysosomal cell complex as a stress response indicator

86%

Kolataj A. , Sliwa-Jozwik A. , Jozwik A.

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tom 19

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nr 3

10

Modes of inhibition of cysteine proteases

86%

Rzychon M. , Chmiel D. , Stec-Niemczyk J.

Acta Biochimica Polonica

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2004

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tom 51

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nr 4

EN

Cysteine proteases are involved in many physiological processes and their hyperactivity may lead to severe diseases. Nature has developed various strategies to protect cells and whole organisms against undesired proteolysis. One of them is the control of proteolytic activity by inhibition. This paper presents the mechanisms underlying the action of proteinaceous inhibitors of cysteine proteinases and covers propeptides binding backwards relative to the substrate or distorting the protease catalytic centre similarly to serpins, the p35 protein binding covalently to the enzyme, and cystatins that are exosite binding inhibitors. The paper also discusses tyropins and chagasins that, although unrelated to cystatins, inhibit cysteine proteinases by a similar mechanism, as well as inhibitors of the apoptosis protein family that bind in a direction opposite to that of the substrate, similarly to profragments. Special attention is given to staphostatins, a novel family of inhibitors acting in an unusual manner.

11

Suitability of electrophoretic analysis [SDS-PAGE, IEF] for estimating the maturity of ripening cheeses

86%

Darewicz M. , Mioduszewska H. , Iwaniak A. , Chojnowski W.

Natural Sciences

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1999

|

tom 03

12

Alterations in the susceptibility to trypsinolysis of proteins chlorinated and- or exposed to SIN-1

86%

Sieradzka-Fleituch M. , Pajdak W.

Current Topics in Biophysics

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1994

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tom 18

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nr 2

13

Influences of pH on proteolysis in water solutions of milk protein concentrate and composition of rennet gels

86%

Zbikowska A. , Szerszunowicz I.

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tom 11

14

Computer simulation of animal proteins proteolysis in the aspect of bioactive peptides obtaining

86%

Dziuba J. , Iwaniak A. , Darewicz M. , Niklewicz M.

Polish Journal of Natural Sciences

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2002

|

tom 10

15

Caspase and calpain mediated proteolysis of nonerythroid spectrin subunits in neuronal cells undergoing apaptosis

86%

Wang K. K. W. , Rathna N. , Rand P. , McGinnis K. , Glantz S. B. , Morrow J. S.

Cellular and Molecular Biology Letters

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1998

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tom 03

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nr 2

16

Effect of null mutations in dnaK and dnaJ genes on conjugational DNA transfer, proteolysis and novobiocin susceptibility of Escherichia coli

86%

Modrzewska M. , Karpinski P. , Grudniak A. , Wolska K. I.

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2002

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tom 51

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nr 3

17

Influence of proteolitic proteasome activity inhibitors on Chara vulgaris spermiogenesis

86%

Kwiatkowska A. , Wojtczak A. , Poplonska K. , Krupa A. , Wlodarczyk J. , Zabka A.

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tom 42

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nr 1

18

Role of ubiquitin ligases in neural stem and progenitor cells

72%

Naujokat C.

Archivum Immunologiae et Therapiae Experimentalis

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2009

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tom 57

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nr 3

177-188

19

Wpływ różnych systemów ochrony chemicznej stosowanych w uprawie ziemniaka na przemiany związków azotowych w glebie. Część I. Doświadczenie modelowe

72%

Przybulewska K. , Nowak A.

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2003

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tom Vol. 10, nr S2

219-231

PL

Określono wpływ różnych systemów ochrony chemicznej stosowanych w uprawie ziemniaka na przemiany związków azotowych w glebie. Badania przeprowadzono, mierząc ilość wytwarzanych przez nitryfikatory azotanów, liczebność tych bakterii (NPL), a także liczebność mikroorganizmów proteolitycznych i bakterii wiążących azot atmosferyczny. Uzyskane wyniki wskazują na zmniejszanie się intensywności nitryfikacji pod wpływem zastosowanych kombinacji pestycydów, wyrażające się mniejszą ilością utlenianego azotu, a także spadkiem liczebności nitryfikatorów w glebie. Użyte zestawy pestycydów działały stymulująco na mikroorganizmy, biorące udział w hydrolizie białka, a silnie hamowały wzrost i rozwój bakterii z rodzaju Azotobacter.

EN

The influence of different systems of chemical plant protection on the transformation of nitrate compounds in soil was described. The investigation was done by measuring amount of nitrate produced by nitrifying bacteria, count (MPN) of this organisms and the number of proteolytic microorganisms and bacteria able to the fixation of atmospherically nitrogen. Obtained results show a decrease in the nitrification intensity under the influence of used combinations of pesticides. lt is expressed with the smaller quantity of oxidized nitrogen and also with the decrease of the number of nitrifying bacteria in soil. Used sets of pesticides had stimulatory effect on the microorganisms active in the hydrolysis of protein and strongly delayed the growth and development of bacteria from genus Azotobacter.

20

Effect of chill storage time on proteolysis and lipid oxidation in vacuum-packed muscles from duck

72%

Przysiezna E.

Polish Journal of Food and Nutrition Sciences

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2007

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tom 57

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nr 4

457-463

EN

Proteolysis and lipid oxidation in the vacuum-packed leg and breast muscles from Mullard drakes stored at 1°C were studied. As proteolysis indicators there were determined proteolytic activity, contents of amino nitrogen and free amino acids (FAA), and TBARS values as indicators of oxidative changes. Changes were also determined in the proteolytic activity, TBARS values and contents of: amino nitrogen and FAA. As a result, 18 FAA were found in breast muscles and 19 in leg and their total contents after 1 day of storage were 184.39 mg/100 g tissue, and 233.33 mg/100 g tissue (respectively). In the case of breast muscles a significant increase in the content of detected FAA (except Pro) was noted after 13 days of storage and in the leg muscles (except Asp) after 5 days of storage. It was established that the proteolytic activity decreased (ca. 38%) in breast after 18 days and in leg (ca. 30%) after 5 days of storage. The content of amino nitrogen significantly increased in breast muscles after 18 days and in leg muscles after 5 days of storage. During storage, TBARS values were observed to increase continuously in breast muscles, whereas in legs they first increased after 5 days and then were observed to decrease.

PL

Badano proteolizę i utlenianie lipidów w próżniowo zapakowanych mięśniach nóg i piersi kaczorów Mullard przechowywanych w temperaturze 1°C. Oznaczono: zmiany aktywności proteolitycznej, wskaźnika TBARS i zawartości: azotu aminowego i wolnych aminokwasów (tab. 1). Zidentyfikowano 18 wolnych aminokwasów w mięśniach piersiowych (rys. 1) i 19 w mięśniach nóg (rys. 2) po 1 dniu przechowywania, a ich całkowita zawartość wynosiła 184,39 mg/100 g tkanki w mięśniach piersiowych i 233,33 mg/100 g tkanki w mięśniach nóg. Istotny wzrost zawartości indywidualnych aminokwasów w mięśniach piersiowych (za wyjątkiem Pro) zaobserwowano po 13 (rys. 1), a w mięśniach nóg (za wyjątkiem Asp) po 5 dniach przechowywania (rys. 2). Podczas przechowywania stwierdzono spadek aktywności proteolitycznej (ok. 38%) w mięśniach piersiowych (tab. 1) po 18, a w mięśniach (ok. 30%) nóg po 5 dniach (tab. 1). Istotny wzrost zawartości azotu aminowego stwierdzono po 18 dniach w mięśniach piersiowych i po 5 w mięśniach nóg (tab. 1). Wskaźnik TBARS wzrastał w mięśniach piersiowych przez cały czas przechowywania, natomiast w mięśniach nóg zaobserwowano jego wzrost po 5 dniach, a następnie spadek (tab. 1).