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EN
Bifidobacteria are considered to be probiotics that exist in the large intestine and are helpful to maintain human health. Oral administration of bifidobacteria may be effective in improving the intestinal flora and environment, stimulating the immune response and possibly preventing cancer. However, for consistent and positive results, further well-controlled studies are urgently needed to describe the basic mechanisms of this microorganism. Analysis of the proteasome-lacking Bifidobacterium longum genome reveals that it possesses a gene, IPR003593 AAA ATPase core, which codes a 56 kDa protein containing one AAA ATPase domain. Phylogenetic classification made by CLANS, positioned this sequence into the ARC divergent branch of the AAA ATPase family of proteins. N-terminal analysis of the sequence indicates this protein is closely related to other ATPases such as the Rhodococcus erythropolis ARC, Archaeoglobus fulgidus PAN, Mycobacterium tuberculosis Mpa and the human proteasomal Rpt1 subunit. This gene was cloned, the full-length recombinant protein was overexpressed in Escherichia coli, purified as a high-molecular size complex and named Bl-ARC. Enzymatic characterization showed that Bl-ARC ATPase is active, Mg+2-dependent and sensitive to N-ethylmaleimide. Gene organization positions bl-arc in a region flanked by a cluster of genes that includes pup, dop and pafA genes. These findings point to a possible function as a chaperone in the degradation pathway via pupylation.
EN
Background: Non-alcoholic fatty liver disease (NAFLD) is one of the most common liver diseases, influenced by various risk factors associated with metabolic disorders. Currently there is no specific effective treatment for NAFLD. Probiotics have been extensively researched for their health benefit Probiotic Supplementation for NAFLD. Methods: A simple literature review was performed based on searches via PubMed, ScienceDirect and Google Scholar, using the keywords "probiotic," "microbiota," "non-alcoholic fatty liver disease," "metabolic disorder," and "therapy." Results: Research on the use of probiotics for NAFLD demonstrated improvement in liver function and histology. However, the literature is inconsistent regarding the probiotics’ influence on the NAFLD risk factors. Probiotics can be an alternative therapy for NAFLD through the ability to modulate the microbiota of the gastrointestinal tract. Conclusion: Probiotics can be an alternative therapy in NAFLD patients, however more extensive studies are needed to ensure the safety of this approach.
3
Content available The impact of polyphenols on Bifidobacterium growth
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EN
Polyphenols are a common group of plant based bioactive compounds, that can affect human health because of their antioxidant and antimicrobial properties as well as free-radical scavenging activity. An increasing interest is observed in the interaction between polyphenols and microbiota occurring in food and the human gut. The aim of the work presented here, was to evaluate the effect of some polyphenolic compounds on the growth of two strains of Bifidobacterium: B. adolescentis and B. bifidum. The influence of some flavonoids: naringinin, hesperidin, rutin, quercetin as well as phenolic acids: gallic, caffeic, p-coumaric, ferulic, chlorogenic, vanillic and sinapic was determined by a 96-well microtiter plate assay. In the experiments the effect of three different concentrations of polyphenols: 2, 20 and 100 µg/ml on the growth of Bifidobacterium strains was investigated. All tested compounds influenced the growth of the examined bacteria. Both stimulatory and inhibitory effects were observed in comparison to the positive control. The strongest impact on the growth of bifidobacteria was observed during the first hours of incubation. The constant inhibitory effect was observed for hesperidin and quercetin addition and was dose-dependent. B. bifidum showed a stronger dependence on phenolic acids content in the medium than B. adolescentis during the first hours of incubation.
EN
There is very limited aquaculture research on candidate probiotics and their effects on fish physiology. In this study, acute applications of four different molecularly identified bacterial species – Brevibacillus brevis FD-1 (A), Pseudomonas fluorescens FDG-37 (B), Bacillus sphaericus FD-48 (C), and B. amyloliquefaciens TV-17C (D), with potential in aquaculture, were tested in rainbow trout (Oncorhynchus mykiss) under static conditions. Physiological changes in blood tissue [hematological indices: erythrocyte count (RBC), leukocyte count (WBC), hemoglobin (Hb), hematocrit (Hct), platelet count (PLT), mean cell hemoglobin concentration (MCHC), mean cell hemoglobin (MCH), and mean cell volume (MCV)], oxidative stress responses in liver and gill tissues [malondialdehyde (MDA) level, antioxidant enzyme activities: superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), glutathione reductase (GR), glutathione-S-transferase (GST), glucose-6-phosphate dehydrogenase (G6PD)] and acetylcholinesterase (AChE) activity in brain tissue (as neurotoxic biomarker) were investigated. Additionally, behavioral differences were recorded by measuring swimming performance to support neurotoxic findings in all treatment groups. The LC5024 value of FDG-37 strain was determined through analysis as 1.0 × 108CFU ml-1. Inhibition of enzyme activity, increase in the MDA level, as well as significant differences in hematological indices and swimming performance were determined in rainbow trout treated with B compared to control and other bacterial groups in gills. The potential for using group FD-48 and TV-17C bacterial strains as probiotics in aquaculture is more pertinent when considering the research findings and water quality parameters.
7
Content available Probiotics in gynaecology
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EN
Probiotics are the bacterial strains living and reproducing in the gastrointestinal tract. They favorably influence human organism by maintaing the balance of intestinal microflora and vaginal biocenosis. Recently, the interest in probiotics bacteria has increased. They are used not only in prophylaxis, but also in treatment, especially in infectious diseases of gastrointestinal and genitourinary tracts.
PL
Probiotyki to szczepy bakterii, które mają zdolność przeżycia i namnażania się w przewodzie pokarmowym oraz działają korzystnie na organizm człowieka poprzez wpływ na równowagę mikroflory jelitowej i biocenozy pochwy. W ostatnich latach nastąpił wzrost zainteresowania bakteriami probiotycznymi, które oprócz wykorzystania w profilaktyce znajdują zastosowanie w lecznictwie, zwłaszcza w chorobach infekcyjnych przewodu pokarmowego i zakażeniach układu moczowo-płciowego u kobiet.
EN
The study presents an assessment of selected mechanical properties of peel and flesh in raw field-grown cucumbers (Cucumis sativus L.), as well as changes occurring in the fruit during the process of pickling, with the use of two types of brine with different composition and with addition of a probiotic. Contents of water in two size fractions of fresh cucumbers were determined using oven-drying method. A puncture test performed with a 5 mm punch probe measured peel and flesh puncture strength and energy as well as apparent modulus of elasticity in raw cucumbers, and following 10, 30, 60 and 90 days of the pickling process. The specified mechanical parameters of cucumber peel and flesh were found to decrease during the pickling process. The reduction in peel and flesh puncture strength is described by a logarithmic function, in energy by linear function and in modulus of elasticity by a power function. The decrease in the puncture strength was slower in the cucumbers subjected to pickling in the brine containing probiotic (B), the decrease in energy was slightly faster in smaller cucumbers (fraction I) subjected to pickling, and during the pickling process in the probiotic containing brine.
EN
The growing popularity of functional foods causes increasing interest in raw materials, which can raise the pro-health value of food when supplemented. The aim of the study was to assess the effect of compounds with antioxidant properties on Lactobacillus casei, as a representative of probiotic microorganisms. In the experiments the pure antioxidants and plant extracts were used. The results showed that catechin at concentration of 100-400 μM and chlorogenic acid (400 μM) had a stimulatory effect on L. casei growth. Quercetin at concentrations of 25-50 μM showed an inhibitory effect when incubation time was ≥6 h. The lingonberry leaves extract caused a slight increase in the dry substance of biomass in comparison with control. Extracts of coffee, chokeberry, and dog rose should not be used as additives for probiotic food as they significantly inhibited L. casei. We conclude that: 1) antioxidants at concentrations higher than 100 μM may stimulate growth of L. casei; 2) except the antioxidants, some other compounds which are present in plants extracts e.g. tannins and alkaloids may exert an antibacterial influence; 3) the probiotic yoghurts supplementation with fruit and other plant materials should be preceded by careful studies about their influence on the bacteria
PL
Rosnąca popularność żywności funkcjonalnej wpływa na wzrost zainteresowania surowcami, które dodane do żywności mogłyby podnieść jej wartość prozdrowotną. Celem badań było ocenienie wpływu związków o właściwościach przeciwutleniających na L. casei jako przedstawiciela drobnoustrojów probiotycznych. Na podstawie wyników badań stwierdzono, że: 1) przeciwutleniacze w stężeniach wyższych niż 100 pM mogą stymulować wzrost L. casei, 2) antybakteryjnie mogą działać nie tylko antyoksydanty, lecz także inne składniki obecne w ekstraktach roślinnych, np. taniny i alkaloidy, 3) suplementacja jogurtów probiotycznych owocami lub innymi surowcami roślinnymi powinna być poprzedzona szczegółowymi badaniami nad ich wpływem na bakterie.
PL
Istotną grupą prozdrowotnych produktów spożywczych są przetwory fermentowane zawierające probiotyki. Pozytywne działanie probiotyków wiąże się głównie z ich zdolnością do zapobiegania niektórym schorzeniom układu pokarmowego i immunologicznego lub zmniejszania częstotliwości ich występowania oraz łagodzeniem przebiegu tych schorzeń. Zgodnie z zaleceniami WHO/FAO z 2001 r. prozdrowotne działanie probiotyków powinno być wykazane i udokumentowane badaniami naukowymi. Niniejszy artykuł przybliża sposoby wykazywania pozytywnych skutków oddziaływania probiotyków na organizm człowieka.
EN
Major group of health-promoting foods are fermented products containing probiotics. Beneficial effects of probiotics on human health are provided by prevention, decreasing occurrence and soothing outcome of several illnesses associated with gastrointestinal tract and immune responses. According to WHO/FAO guidelines from 2001 year probiotics health-claims need to be supported by scientific evidence. Here we describe selected examples of scientific approaches to verify probiotic influence on human organism.
EN
Two-color DNA microarrays are commonly used for the analysis of global gene expression. They provide information on relative abundance of thousands of mRNAs. However, the generated data need to be normalized to minimize systematic variations so that biologically significant differences can be more easily identified. A large number of normalization procedures have been proposed and many softwares for microarray data analysis are; available. Here, we have applied two normalization methods (median and loess) from two packages of microarray data analysis softwares. They were examined using a sample data set. We found that the number of genes identified as differentially expressed varied significantly depending on the method applied. The obtained results, i.e. lists of differentially expressed genes, were consistent only when we used median normalization methods. Loess normalization implemented in the two software packages provided less coherent and for some probes even contradictory results.In general, our results provide an additional piece of evidence that the normalization method can profoundly influence final results of DNA microarray-based analysis. The impact of the normalization method depends greatly on the algorithm employed. Consequently, the normalization procedure must be carefully considered and optimized for each individual data set.
EN
The problem of soil contamination is relevant today. Soil contamination is generally associated with intensive industrial activities, inadequate waste management, mining, military activities, or accidents. Pollutants (e.g. heavy metals) are accumulating in soil and have major indirect impacts on biodiversity, quality of groundwater resources, and food safety. Soil contamination of agricultural land is a particular threat. Due to the military action and other conditions, the numbers of sites where potentially polluting activities have taken place are increasing in Ukraine. The cultivation of agricultural crops on soils with a medium or high level of toxicity is only possible after the remediation of this area. The use of biological methods to intensify soil remediation processes, thereby reducing the additional burden on the environment, is becoming more widespread. The application of probiotics is an innovative and ecological method for the restoration of contaminated soils. This will enable to improve the conceptual approaches to the implementation of an ecologically safe model for the restoration of contaminated land under the conditions of military action in Ukraine. The aim of the research was to assess the phytotoxic effect of soil contaminated with heavy metals and petroleum products before and after probiotic application. The seedling method was used to determine the remediation potential of contaminated soils. An express test of Triticum aestivum was used to determine soil phytotoxicity. The research results show the negative impact of soil contamination with heavy metals and petroleum products on the biometric indices of Triticum aestivum plants and positive dynamics of biometric indices of Triticum aestivum in variants after probiotic treatment (86–92% compared to the control). The obtained data show that a significant phytotoxic effect is observed by all biometric indices of Triticum aestivum in all studied variants. The highest phytotoxic effect (33.56–42.70%) was observed in variants with combined contamination (PP+Zn+Pb). The results of probiotic application show a phytotoxic effect of less than 20% by all biometric indices of Triticum aestivum for all studied variants. Therefore, the results of the research can be used to develop recommendations for the remediation of land contaminated by military actions in Ukraine and the creation of sustainable agroecosystems.
EN
Two-color DNA microarrays are commonly used for the analysis of global gene expression. They provide information on relative abundance of thousands of mRNAs. However, the generated data need to be normalized to minimize systematic variations so that biologically significant differences can be more easily identified. A large number of normalization procedures have been proposed and many softwares for microarray data analysis are available. Here, we have applied two normalization methods (median and loess) from two packages of microarray data analysis softwares. They were examined using a sample data set. We found that the number of genes identified as differentially expressed varied significantly depending on the method applied. The obtained results, i.e. lists of differentially expressed genes, were consistent only when we used median normalization methods. Loess normalization implemented in the two software packages provided less coherent and for some probes even contradictory results. In general, our results provide an additional piece of evidence that the normalization method can profoundly influence final results of DNA microarray-based analysis. The impact of the normalization method depends greatly on the algorithm employed. Consequently, the normalization procedure must be carefully considered and optimized for each individual data set.
EN
This study was conducted to determine the effect of probiotic addition in commercial feed to the growth and survival rate of Sangkuriang catfish (Clarias gariepinus). This research was conducted at the Ciparanje Experimental Pond Laboratory, Faculty of Fisheries and Marine Sciences, Universitas Padjadjaran, Jatinangor, West Java. The method in used this research was Completely Randomized Design with four treatments and four replications. The treatment was the variances of probiotic addition which consisted of control (without probiotics), probiotic addition at as much as 0.5 grams / kg of feed, 1 gram / kg of feed and 1.5 grams / kg of feed. The parameters observed were specific growth rate (SGR), absolute biomass, survival rate (SR) and feed conversion ratio (FCR). Data were analyzed using Variant Analysis, at 95% confidence level, and continued with Duncan's Multiple Range Test. The results showed that the increase in the daily growth rate of Sangkuriang catfish was straight-line with the addition of probiotics. Furthermore, commercial probiotics with a dose of 1 gram / kg of feed resulted in specific growth rate of 4.22%, absolute weight of 11 grams, survival rate (SR) of 97.5% and FCR of 0.89.
EN
ObjectivesThe aim of this study was to observe the depression-like behavior changes of rats exposed to lead with or without probiotic intervention, and to investigate changes in the gut microbiota and fecal short-chain fatty acids (SCFAs) levels after lead exposure, and the possible functions of probiotics in this process.Material and MethodsAdult male Sprague Dawley rats were exposed to a 300 mg/l lead acetate solution for 24 weeks, with or without probiotic (freeze-dried powder containing Lactobacillus and Bifidobacterium: 6 billion live bacteria/2 g) intervention in weeks 17–24. The sucrose preference test (SPT), the forced swim test (FST), and the tail suspension test (TST) were preformed to study the depression-like behaviors of these rats. The alteration of rat gut microbiota induced by lead exposure was analyzed by 16S rRNA sequencing, and the levels of fecal SCFAs were detected using gas chromatography.ResultsNeurobehavioral tests showed that lead exposure induced depression-like behavior in rats, including reduced sucrose preference in the SPT, and increased immobility times in the FST and the TST. Sequencing and gas chromatography showed that lead exposure changed the structure and the phylogenetic diversity of the gut microbiota, as well as significantly altered the levels of SCFAs. Moreover, the depression-like behaviors, and the changes in both gut microbiota and SCFAs, could be mitigated by probiotic intervention.ConclusionsLead exposure not only changes the structure and diversity of the gut microbiome but also affects metabolic function. Probiotic intervention may be a novel initiative for the prevention and treatment of neurological damage following lead exposure. Health. 2022;35(1):95–106
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