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tom nr 3
71--82
EN
This paper presents the results of research on the bacteriological quality of rainwater collected directly from precipitation and various roof surfaces – concrete and ceramic roof tiles, galvanised sheet and a terrace covered with epoxy resin. Samples for the research were collected in a suburban area in 2015-2016. The quality of rainwater was assessed based on the results of bacteriological analyses (the presence of indicator bacteria and the total mesophilic and psychrophilic bacteria count). The effect of the seasons and the type of roofing on the microorganism count was discussed. Significant differences in the quality of rainwater collected from various roof surfaces, related to the roughness and chemical composition of the materials covering the roofs were indicated. In the rainwater examined, significant numbers of potentially pathogenic microorganisms such as Escherichia coli, coliforms and faecal streptococci were detected. The results obtained were the basis for indicating the possibility of using rainwater for various household purposes. Rainwater is not suitable for uses requiring the quality of water intended for consumption due to a great number of psychrophilic, mesophilic and faecal bacteria.
EN
Slurry often contains many pathogenic microorganisms, which are dangerous to human and animal health. The aim of study was to estimate the elimination rate of indicators and pathogenic bacteria – Escherichia coli, fecal streptococci D-group and Salmonella senftenberg W775 in peat soil according to soil storage temperature and slurry addition. The elimination rate of fecal bacteria was more effective in 20°C than in 4°C. The slowest reduction of the fecal bacteria was observed in soil with slurry. The salmonellas were eliminated more slowly in comparison to another group of investigated microorganisms. The fastest reduction of streptococci was estimated in 20oC, however E.coli in 4°C. To minimize the hazard for environment, slurry should be subjected to monitoring investigation before use on arable land.
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EN
Human organism, constantly exposed to a large variety of pathogenic microorganisms and their products, such as lipopolysaccharide (LPS), developed innate immunity as a fi rst line of defence. One of the compartments of our organism well equipped with these defence mechanisms is the respiratory system. The cells lining the airways respond to the presence of virulent microorganisms by producing natural antimicrobial peptides, including the only member of the cathelicidins family found to date in humans, peptide LL-37. LL-37 is a small peptide of 37 amino acid residues. The peptide, in addition to its bactericidal effect, plays numerous roles in infl ammatory and tissue remodelling processes. It stimulates angiogenesis, induces proliferation of lung epithelial cells, accelerates wound closure of the airway epithelium, and provokes cytokine release (e.g. IL-8) and cell migration. LL-37 is also able to neutralize LPS, a heteropolymer associated with organic dust, produced by Gram-negative bacteria. LPS (commonly referred to as endotoxin) plays an important role in pathogenesis of many respiratory diseases caused by organic dust, including organic dust toxic syndrome and chronic illnesses such as chronic obstructive pulmonary disease (COPD), asthma or allergic alveolitis (hypersensitivity pneumonitis). LPS is a strong pro-infl ammatory stimulus, inducing in respiratory airways expression of antimicrobial peptides, including LL-37, which is in turn a potent LPS-neutralizing factor. The article discusses the complex interplay between endotoxin and the LPS-neutralizing, pleiotropic peptide LL-37 in pathogenic mechanisms of lung diseases, with regard to closer perspectives of using LL-37 and its derivatives as therapeutic agents.
PL
Badano występowanie bakterii chorobotwórczych: pałeczek Salmonella oraz Listeria monocytogenes w różnych rodzajach środków spożywczych pochodzących z obrotu, z rynku warszawskiego. Zbadano 126 próbek różnych rodzajów żywności w tym nietrwałej, jak surówki warzywne, sery twarogowe, kiełbasy surowe, mięso mielone, ciastka oraz o przedłużonym okresie trwałości jak koncentraty i przyprawy. Pałeczki Salmonella w 25 g wykryto w dwóch próbkach mięsa mielonego drobiowego z indyka i w jednej próbce ciastek. Obecność Listeria monocytogenes w 10 g stwierdzono w czterech próbkach: w kiełkach mieszanych, kiełbasie „ metka tatarska ", białej kiełbasie i mrożonym hamburgerze wołowym.
EN
126 samples of different kinds of food were tested for Salmonella and Listeria monocytogenes. The samples were taken from Warsaw market. In three samples of raw poultry meat and one sample of cake - poppy-seed, Salmonella Enteritidis was detected. In one sample of raw poultry meat Salmonella Hadar was spotted. In 4 of samples: routs, „metka sausage", raw white sausage and frost beef hamburger the presence of Listeria monocytogenes in 10 g was detected.
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