Wyniki wyszukiwania - Biblioteka Nauki

1

Effect of cold storage of raw milk on milk protein system

100%

Sienkiewicz T. , Gebhardt U. , Krenkel K.

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nr 2 Suppl.

2

Effect of prolactin receptor (PRLR) and beta-casein (CSN2) gene polymorphism on the chemical composition of milk sows

86%

Skrzypczak E. , Babicz M. , Pastwa M.

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nr 2

3

An estimation of the genetic distance between Polish Red and other Red Cattle breeds on the basis of selected milk protein loci

86%

Zaton-Dobrowolska M. , Citek J. , Filistowicz A. , Rehout V. , Szulc T.

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nr 2

EN

The material for the study were six populations of Red Cattle, including three samples of the Polish Red cattle population (PC’69, n = 203, PC’82, n = 261 and PC’96, n = 313) and one each of the Danish Red (DC, n = 169), German Red (NC, n = 510) and Czech Red (CC, n = 14). DNA was isolated from blood, according to the method by Gemmell and Akiyama (1996). All individuals were genotyping for the following proteins: a S1-casein, b-casein, k-casein and b-lactoglobulin. The frequency of alleles was estimate and was used for the estimation of the genetic distance between populations. Using the PHYLIP ver. 3.5c software the genetic distance was determined according to 1) Nei, 2) Cavali-Sforza and 3) Reynolds. All the populations demonstrated a similar distribution of allele frequencies at individual loci. Only the Czech Red showed different tendencies as regards b- and k-casein and b -lactoglobulin. This may have been the effect of the smaller population size as compared to the remaining breeds. Genetic distances estimated by these three using methods were similar. The smallest genetic distance was observed between populations PC96 and NC (0.0007). In turn, the highest value was obtained between population DC and CC (0.0776). In the case of the genetic distance calculated according to the formulas by Cavalli-Sforza and Reynolds, the values obtained were higher then value of genetic distance estimated by Nei. The comparatively low values for the genetic distance obtained in the present studies may indicate that the breeds analysed originate from a common ancestor or lived on neighbouring geographical regions, what could lead to a frequent exchange of genes. The considerable similarity between those breeds could also be caused by a similarly conducted selection, based on similar assumptions (similar type of production). Those populations demonstrate similar gene pools what may be used for common preserve breeding programmes, which would decrease the danger of inbreeding. An analysis of the data collected over 30 years for the three populations of the Polish Red cattle, renders it possible to conclude that the frequency of individual alleles of the proteins examined are subjected to a small but continuous change.

4

The influence of casein micelle size on selected functional properties of bulk milk proteins

86%

Zbikowska A. , Dziuba J. , Jaworska H. , Zaborniak A.

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nr 1

5

Correlations between genetic variants of milk proteins and milk yield and composition in first full lactations of Black-and-White cows

86%

Pytlewski J. , Antkowiak I. , Dorynek Z.

Animal Science. Scientific Papers of Agricultural University of Poznan

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2002

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tom 04

6

Selected genetic markers: their effect on milk production traits

86%

Cernekova V. , Dudkova G. , Kott T. , Stankova Z. , Soldat J. , Markova M.

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tom 22

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nr 2

7

Genetic variation of milk proteins in cattle maintained as gene reserve

86%

Blahova B. , Rehout V. , Kubek A. , Citek J. , Pubalova M.

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tom 22

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nr 2

8

Structural aspects of functional properties of milk proteins

86%

Dziuba J. , Darewicz M.

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2000

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tom 04

9

Characteristics of milk from different species of farm animals with special emphasis on health-promoting ingredients

72%

Stobiecka M. , Krol J. , Brodziak A. , Wajs J.

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nr 3

10

Dairy traits of the progeny of Red-and-White bulls described through the genetic polymorphism of milk proteins and principally of fraction alphaS1-CN

72%

Dobicki A. , Walawski K. , Chladek G. , Nowopolska A. , Koliski W. , Zachwieja A.

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nr 1

11

Single nucleotide polymorphism database of candidate genes associated with cow milk protein biosynthesis

72%

Kaminski S. , Rusc A. , Malewski T.

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tom 13

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nr 1

12

Intensity and causes of culling and lifetime productivity of cows in terms of dairy cattle herd-book registration

72%

Pytlewski J. , Antkowiak I.

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tom 55

13

Polymorphism of main milk proteins in beef cattle maintained in East-Central Poland

72%

Litwinczuk Z. , Krol J.

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tom 20

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nr 1

14

Changes in DNA-binding activity of transcription factors in the differentiating bovine mammary gland

72%

Malewski T. , Gajewska M. , Zwierzchowski L.

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tom 23

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nr 2

EN

Our earlier studies carried out in silico demonstrated that different transcription factors have their putative binding sites in the 5’-flanking regions of bovine milk protein genes. Now we extended our study to include the experimental analysis of these transcription factors. This study on electrophoretic mobility shift assay (EMSA) of nuclear proteins derived from bovine mammary glands showed for the first time the presence there of CREB, NF-κB, Oct1 and Sp1 transcription factors. The pattern of DNA-protein complexes of Sp1 transcription factor significantly differed between virgin heifers and lactating cows. Transition from pregnancy to lactation was associated with changes in the DNAprotein binding patterns of NFI and Oct1 transcription factors.

PL

Wcześniejsze badania autorów prowadzone in silico wykazały obecność potencjalnych miejsc wiązania dla wielu czynników transkrypcyjnych w rejonach 5’-flankujących genów białek mleka. W obecnej pracy przedstawiono wyniki analizy poszerzonej o doświadczalne badanie wiązania tych czynników transkrypcyjnych z DNA. Stosując analizę EMSA z użyciem białek jądrowych gruczołu mlekowego bydła w różnych stadiach jego rozwoju, po raz pierwszy wykazano obecność w nim czynników transkrypcyjnych CREB, NF-κB, Oct1 i Sp1. Kompleksy DNA-białko czynnika transkrypcyjnego Sp1 wykazały różne wzory ruchliwości elektroforetycznej u dziewiczych i cielnych jałówek. Przejście od ciąży do laktacji wiązało się z dalszymi zmianami w kompleksach DNA-białko czynników transkrypcyjnych NFI i Oct1.

15

Associations between bovine milk protein genotypes and haplotypes and the breeding value of Polish Black-and-White bulls

72%

Kaminski S. , Rymkiewicz-Schymczyk J. , Wojcik E. , Rusc A.

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nr 2

16

Gospodarka tluszczem i bialkiem mleka w wybranych spoldzielniach mleczarskich wojewodztwa olsztynskiego

58%

Sokolowski T. , Bazydlo I.

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1994

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tom 29

79-89

EN

Utilization of milk fat and protein in six dairy cooperatives of Olsztyn vojevodship in years 1990 and 1991, was investigated. There was found, that in the investigated period of time amount of purchased milk decreased from 10.1 to 17.3% in four dairy cooperatives, and increased from 6.9 to 10.6% in another two cooperatives. Because of different production rentability und lower consumption of dairy products the changes of production profile occurred. The significant production drop of many food products was observed, mainly dring milk. During investigated period of time, the total amount of milk fat as well that one utilized for food and feed went down in the investigated dairy cooperatives, and the milk protein utilization was even lower than fat. Amount of protein for food production was lower and indicators of total protein utilization were lower in four of six investigated cooperatives. The obtained results indicated that the influence of rational management of milk components on financial results of dairy cooperatives is underestimated.

PL

Badano wykorzystanie tłuszczu i białka mleka w sześciu spółdzielniach mleczarskich woj. olsztyńskiego w latach 1990 i 1991. Stwierdzono, że w badanym okresie skup mleka obniżył się w czterech spółdzielniach o 10.1-17.3%, a w pozostałych wzrósł o 6.9-10.6%. W związku z różną opłacalnością produkcji i obniżeniem spożycia artykułów mleczarskich wystąpiły zmiany struktury asortymentowej produkcji. Nastąpił spadek produkcji wielu artykułów spożywczych, w tym głównie mleka spożywczego. W badanym okresie ilość tłuszczu mlekowego wykorzystanego na cele spożywcze, paszowe i ogółem obniżyła się w badanych spółdzielniach, a wykorzystanie białka mleka było niższe niż tłuszczu, Ilość białka na cele spożywcze w pięciu spółdzielniach się obniżyła. Wskaźniki wykorzystania białka ogółem obniżyły się w czterech spółdzielniach. Wskazano, że nie docenia się wpływu racjonalnej gospodarki składnikami mleka na wynik finansowy w spółdzielniach mleczarskich.

17

Wplyw dodatku roznych preparatow bialek mleka oraz roznych warunkow homogenizacji na stabilnosc rekombinowanych emulsji typu o-w [smietanka]

58%

Juskiewicz M. , Kisza J. , Kubiak A.

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nr 29

57-78

18

Fluorine and zinc distribution in selected rats tissues after supplementation of feed with milk proteins and inhalation exposure to ammonium fluoride

58%

Machoy-Mokrzynska A. , Borowiak K. S.

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nr 2

19

Opioid activity of high-protein supplement for sportsmen

58%

Wocior A. , Iwan M. , Kostyra H. , Kostyra E.

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nr 2

20

MilkProtChip - a microarray of SNPs in candidate genes associated with milk protein biosynthesis - development and validation

58%

Kaminski S. , Ahman A. , Rusc A. , Wojcik E. , Malewski T.

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nr 1

EN

MilkProtChip is an oligonucleotide microarray based on the arrayed primer extension (APEX) technique, allowing genotyping of single nucleotide polymorphisms (SNPs) in genes of interest for bovine milk protein biosynthesis. APEX consists of a sequencing reaction primed by an oligonucleotide anchored with its 5’end to a glass slide and terminating one nucleotide before the polymorphic site. The extension with one fluorescently labeled dideoxy nucleotide complementary to the template reveals the polymorphism. A total of 75 SNPs were selected among those associated directly or potentially with milk protein content. Among the 75 SNPs, 4 did not produce a positive signal. Most of the remaining SNPs produced a signal for both strands, except for 4 (one strand). In the validation step, 12 Polish Holstein bulls, 1 Polish Red bull, 1 bison (Bison bonasus), 11 Jersey cows and 25 Polish Holstein cows were screened to validate SNPs. Among the 71 selected SNPs - 26 were found monoallelic, the rest showing at least two genotypes for the entire population under study. All the animals were earlier genotyped for 2-5 SNPs by PCR-RFLP and PCR sequencing and all showed complete concordance with APEX genotyping. APEX reactions showed relatively high signal frequencies: more than 0.9, 0.9-0.8 and below 0.8, for 65,4 and 2 DNA samples, respectively. The primary application of the MilkProtChip is the simultaneous genotyping of dozens of SNPs to reveal and clarify the genetic background of milk protein biosynthesis. The chip may possibly be used for dairy cattle identification and paternity analysis, evolutionary studies, the evaluation of genetic distances between wild and domestic cattle breeds and the domestication history of bovine species.