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Content available remote Type IV resistant starch increases cecum short chain fatty acids level in rats
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EN
Resistant starches are type of dietary fibers. However, their physiological effects depend on the way they resist digestion in the gastrointestinal tract. The objective of this study was to examine the hypothesis that new type of RS4 preparations, of in vitro digestibility of about 50%, obtained by cross-linking and acetylation, acts as a prebiotic by increasing short chain fatty acids content in cecum digesta. The rats were fed with diet containing pregelatinized, cross-linked and acetylated starches as a main carbohydrate source. Pregelatinized, but not chemically modified, potato starch was used in the composition of the control diet. After two weeks of experiment the increase of short chain fatty acids contents in ceceum digesta was observed. The intake of starch A, cross-linked only with adipic acid, resulted in increase of about 40% of short chain fatty acids content, whereas starch PA cross-linked with sodium trimetaphosphate and adipic acid of about 50%. The utmost twofold increase was observed in the case of the production of propionic acid. In contrast, the content of butyric acid increased (12%) only as an effect of consumption of starch PA and even decreased (about 30%) in case of starch A. Both RS4 starches caused an increase of the production of acetic acid by more than 40%. No changes in serum biochemistry, liver cholesterol and organ weights of rats were stated.
EN
We tested the hypothesis that seasonal differences in passage rate and digestibility of native forage in wapiti Cervus elaphus canadensis Erxleben, 1777 were due to seasonal appetence and digestive adaptation as well as plant phenology and compo­sition. To this end, we compared digestive characteristics of seasonal pasture with those of a standard alfalfa-barley pellet fed to wapiti hinds throughout the year. No evidence of seasonal effects independent of forage quality was found. Although dry matter intakes differed 2-3 fold, the digestibility of the standard pelleted diet was not signficantly different in winter (0.68 ± 0.02), spring (0,74 ± 0.01) and summer (0.71 ± 0.01). However, digestibilities of natural forages increased greatly from winter (0.51 ± 0.01), to spring (0.77 ± 0.02), with a gentle decline into autumn (0.65 ± 0.02). Because retention times of pelleted diets were not significantly different among seasons (winter 28 + 1 h, spring 24 ± 2 h, summer 22 ± 1 h), digestive kinetics also seemed to be mainly a function of seasonal composition of native forage rather than specific seasonal adaptation. The mean retention time of winter herbage (41 ± 2 h) was significantly longer than that of spring (28 + 3 h) and summer (25 ± 2 h) forages. Pasture forages were retained longer (51 ± lh) than pellets in winter but not in spring (28 ± 1 h) and summer (27 ± 1 h). Fecal moisture was a good indicator of digesta retention time despite forage type and may provide a simple index of digestive function for field studies.
EN
This study was conducted to determine the effects of Bacillus licheniformis (Bl) and Clostridium butyricum (Cb) and their combinations with yeast culture on in vitro rumen fermentation in a two-way factorial design. Treatments included Bl or Cb at levels of 0, 0.5, 1, 5 and 10 mg and their combination with yeast culture at 0, 18, 27, 36 and 60 mg per 200 mg substrate, respectively. Gas production was recorded after 2, 4, 6, 9, 12, 24, 36, 48 and 72 h incubation. In vitro organic matter digestibility (IVOMD) was estimated by 24 h gas production. Rumen fermentation parameters were determined after 24 h of incubation. Rate constant of gas production was not influenced by Bl or Cb alone, but increased (P<0.05) with inclusion of yeast culture. The IVOMD was influenced (P<0.05) by addition with Bl, Cb or yeast culture, with highest IVOMD observed when Bl or Cb was combined with 60 mg yeast culture. Total volatile fatty acids were affected by Bl and yeast culture (P<0.01), but not by Cb (P>0.05). There were significant interaction effects on pH, acetate to propionate ratio and ammonia-N between yeast culture and Bl or Cb. From the above results, it is indicated that Bl and Cb may be more effective as feed additives when combined with yeast culture than when offered separately.
EN
Protein digestibility may be Influenced by the presence of dietary fibre affecting the nutritional quality of a feed or food product. This study investigated the interplay between rapeseed (Brassica napus L.) protein and fibre constituents separated by industrially scalable pilot plant processing and recombined in mixed samples. Total dietary fibre (TDF) fractions were isolated from rapeseed hulls (TDF-RH) and purifi ed rapeseed embryo fibres (TDF-RE). The effect of TDF sources on in vitro protein digestibility (IVPD) of a rapeseed protein concentrate rich in napin proteins (RP2) was assessed at three inclusion levels (200, 333, and 500 mg/g DM) using a sequential transient proteolysis by pepsin (1 h) and pancreatin (1 h). The IVPD of RP2 was dose-dependently decreased upon addition of hull fibres at all inclusion levels (8.9-26.6%; P<0.05), whereas the effect of embryo fibres was of a markedly lower magnitude and only signifi cant at the medium to high levels (7.3-8.9%; P<0.05). These results demonstrated that TDF fractions obtained from rapeseed differentially affect the protein digestibility of rapeseed napin proteins depending on the fibre source and inclusion level.
EN
The purpose of the study was to determine the effect of a silage additive on maize silage fermentation and assess the relationship of silages with different levels of fermentation end-products to ruminal fermentation parameters and organic matter (OM) digestibility. The maize silage underwent two treatments: a) no additive (control silage), b) additive with Lactobacillus plantarum and Pediococcus acidilacticci and enzyme cellulase. The additive improved silage fermentative processes, preserved more water soluble carbohydrates, increased fermentation of lactic acid and decreased formation of ammonia-N. Two groups of analogous cows were formed: the cows (n = 10) of the control group were fed silage without additives, the cows (n = 10) of the experimental group the silage additive. Rumen fluid from cows was sampled. The silage additive improved fermentation processes in the rumen: the amount of volatile fatty acids, including propionic acid, increased as well as the total and lactate fermenting bacterial count and number of protozoa. The incubation of both grass hay and also silage with the rumen fluid from the cows receiving silage with inoculant and cellulase additive improved their OM digestibility.
EN
The aim of the present study was to investigate the effect of varying levels of whole-grain wheat in the diet (12.5%, 25%, 37.5% and 50%) on the particle size spectra of duodenal digesta and nutrient digestibility in young turkeys. After feeding from 4 to 8 weeks of age, a linear increase in particles larger than 2 mm (P = 0.002) and a linear decrease in particles ranging in size from 1 to 2 mm and smaller than 0.071 mm (P = 0.001; excluding particles ranging in size from 0.106 to 0.071 mm) were noted in the duodenal digesta of turkeys. No differences were observed in the digestibility coefficients of dry matter and crude fibre or in nitrogen retention. Our findings indicate that the inclusion of whole wheat in the diet increased the proportion of coarse particles in the digesta leaving the gizzard, which did not reduce the apparent digestibility of dry matter and crude fibre, or nitrogen retention. Nonetheless, both the weight gain and feed conversion ratio worsened linearly with increasing amounts of whole wheat in the diet (linear contrast P = 0.002 and P = 0.001, respectively)
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