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tom 63
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nr 06
662-666
EN
The blood levels of parathyroid hormone (PTH), calcium, phosphorus and magnesium were examined in 122 cows, which included healthy cows as well as cows with osteomalacia and paresis. Levels of PTH were determined in vitro using IMMULITE analyzer, levels of macroelements - using the automated “Eos-Bravo” analyser with Hospitex reagents. The blood level of PTH ranged from 1.7 to 7.5 pmol/l in healthy cows and from 3.95 to 20.19 pmol/l in sick cows. The highest and most significant increase in blood PTH level (up to 18.31 ± 1.88 pmol/l) was found in cows with parturient paresis. The blood serum levels of PTH in healthy cows changed depending on age and on the physiological status, being significantly lower in cows 2-4-years-old compared with cows 8-years-old and over. The levels of PTH were significantly higher in cows-in-calf and heifers-in-calf compared with cows of the lactation period. Higher levels of PTH were found during the winter than in the summer. The blood level of PTH correlated inversely with the level of calcium in cows with osteomalacia (r = -0.89) and with parturient paresis (r = - 0.49-0.61). The blood serum PTH levels were significantly increased in all groups of cows (p<0.05) on the day of parturition and one day after parturition compared with the measured PTH level 5 days before parturition. Vitamin D (injected 3-5 times) administered during the last days before calving effectively increased the levels of calcium, phosphorus and PTH.
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tom 63
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nr 04
432-434
EN
The aim of this research was to investigate the addition of Calcium source (marble powder) of up to 1.2, 1.4, 1.6% levels on growth performance, feed intake, some organ weights and tibia ash parameters in broiler diets. This research was conducted according to Randomized Plots Design by using 3 groups with 3 replicates between 12 May - 23 June in Çarýklý region of Diyarbakýr. The experiment was conducted on 126 day-old equally sexed Ross 308 broiler chicks. Three diets used as 3 treatments were included (1.2, 1.4 and 1.6% Ca levels) in broiler the diets. Three replicates with 14 equally sexed birds were arranged in each plot. The experiment was started from 7-days-of-age to slaughter weight. The first group was a control and contained 1.2% Ca levels; marble flour was added to the control diet as a Ca source and the Ca levels 1.4 and 1.6% for the 2nd and 3rd groups, respectively. At the end of the trial, data were analysed by MSTAT C program, and means were examined by DUNCANs multiple test. There were no statistically significant differences between the groups for all investigated parameters (Live weight gain, feed intake, feed conversion ratio, carcass parameters and some organ weights) using Ca doses (P > 0.05). However, crude ash and calcium contents of diets were increased by additional Ca levels (P < 0.01), but phosphorus content of diets were decreased by the addition of a calcium source. The results of this study have shown that there is no adverse effect on broiler performance of an additional Ca source up to 1.6% levels.
EN
It is postulated that disturbances in calcium homeostasis play an important role in pathogenesis of Alzheimer’s disease (AD). Changes of neuronal calcium concentration are responsible for the oxidative stress as well as altered metabolism and production of amyloid-beta peptides (Aβ). Aβ may further exacerbate calcium dysregulation, causing synaptic dysfunction, neurodegeneration and cognitive impairment. Recent data indicate that AD is associated with disturbances of circadian rhythm in the patients. However, till now nothing is known about the molecular mechanisms involved in AD-related circadian clock alterations. In our study we investigated the effect of Aβ peptides on the rhythmic oscillation of cytosolic and mitochondrial calcium levels. To investigate molecular clock mechanisms, the studies we carried out in human primary skin fibroblasts, a previously established experimental model. Our data showed circadian rhythm of calcium ions concentration in cytosol and mitochondria. Moreover we observed circadian oscillation of ROS formation and redox potential. Treatment with Aβ fibrils at the concentration of 0.5 µM disturbed cytosolic calcium oscillations and mitochondrial redox state. Studying mechanisms involved in this phenomenon indicated that Aβ did not affect ER calcium stores, but induced changes of calcium influx mediated by purinergic P2X7 receptor. The specific antagonist of P2X7 receptor Brillant Blue G abolished negative impact of Aβ and restored calcium circadian rhythm. Summarizing, our results indicate that Aβ may play a significant role in disturbances of circadian calcium oscillation, suggesting the importance of this phenomenon in ADrelated changes in biological clock. Supported by grants from Sciex 10. 258 to A.K. as well as Swiss National foundation (SNF No 310030_122572) and Synapsis Foundation to A.E.
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tom 545
EN
The distribution and content of calcium ions in organelles, cytoplasm and celi walls of leaf mesophyll and epidermis of Sium latifolium air-water and terrestrial plants at various developmental stage was investigated. It was established that the relative content of Ca2+ in cells changed in relation to growth stage and environmental growth conditions. Moderate water deficit led to the increase of calcium content in chloroplasts and nucleus of palisade mesophyll, and in cell walls of adaxial epidermis of plants that were collected at flowering phase. At fruitage stage, water deficit led to the decrease of Ca2+ in chloroplasts and in cytoplasm of mesophyll cells and in cell walls of epidermis. The obtained results show that these data can be used as a basis for future studies on the mechanisms of calcium redistribution and the role of ion channels in plant cells under water deficit.
PL
Celem badań była analiza zawartości i rozmieszczenia jonów wapnia w organellach, cytoplazmie i ścianach komórkowych mezofilu i epidermy liści dwóch ekoform Sium latifolium (powietrzno-wodna i lądowa) w różnych fazach rozwojowych. Stwierdzono, iż względna zawartość Ca2+ ulega zmianom w zależności od fazy rozwojowej i warunków środowiskowych, w jakich rosną rośliny. Umiarkowany deficyt wody prowadzi do wzrostu zawartości wapnia w chloroplastach i jądrach komórek miękiszu palisadowego i górnej epidermy liści roślin w fazie kwitnienia. Równocześnie u roślin w fazie owocowania, deficyt wody prowadzi do spadku zawartości Ca2+ w chloroplastach i cytoplazmie mezofilu i ścianach komórkowych epidermy liści. Uzyskane rezultaty mogą stanowić podstawę do dalszych badań nad mechanizmem redystrybucji jonów wapnia i roli kanałów jonowych u roślin rosnących w warunkach deficytu wody.
EN
Three phytase preparations were added to broiler rations with considerably reduced levels of phosphorus. Birds were allocated to a positive control group (I), a negative control group (II) and three phytase-supplemented groups (III – Aspergillus niger phytase, IV – Penicillium canescens phytase, V – Pichia phytase). Major components of starter and grower diets were wheat, corn and soybean meals. Starter and grower diets (in meal form) contained 6.65 or 6.01 g of total P/kg and 3.98 or 3.68 g available P/kg in group I, and 5.67 or 5.05 of total P/kg and 2.98 g or 2.70 g available P/kg in groups II – V, respectively. This experiment, performed over a five-week period, involved Ross 308 male chickens kept in battery cages. Each group was divided into 9 subgroups, each of 9 chickens (81 birds per treatment). Phytase efficacy was evaluated based on performance results, carcass quality and bone mineralization. Optimal performance levels were achieved in group I (2.149 g body weight, 1.776 kg feed/kg gain). P reduction in group II decreased weight gains by 9% and increased feed conversion by about 5%. Diet supplementation with phytase in groups III – V compensated for the decrease in performance observed in group II. The effect exerted by three microbial phytases was similar. The difference in carcass weight between group II and the other groups was significant. P reduction in the diets negatively influenced the process of bone mineralization, which was enhanced by phytase supplementation.
PL
Celem pracy była ocena wpływu hydrokortyzonu na poziom wapnia w zębach szczura. Badania wykonano na szczurach rasy Wistar. Poziom wapnia w zębach oznaczono z użyciem absorpcyjnej spektrometrii atomowej. Poziom ten zależał od czasu trwania doświadczenia i rodzaju podawanego leku. Obniżoną w sposób istotny zawartość wapnia stwierdzono w siekaczach górnych szczurów otrzymujących hydrokortyzon.
EN
The aim of the study was to evaluate the influence of hydrocortisone on the level of calcium in rat teeth. Experiments were carried out on Wistar rats. The calcium level in the teeth was marked using the atomic absorption spectrometry. This level was dependent on the duration of the experimental and the type of drug administered. Significant decrease of the calcium concentration in the upper incisors of rats receiving hydrocortisone was found.
EN
The aim of the present study was to find the source of postpollination increase in calcium content in the transmitting tract in the style of Petunia hybrida Hort. Cytochemical and radiographic studies revealed a progressive increase in the level of free and loosely bound calcium ions in the course of successive developmental stages of the pollen tubes in the pistil. A surprising phenomenon was the increase in Ca2+ content in the extracellular regions of the transmitting tract preceding the penetration of the pollen tubes therein. We suggest that the main sources of postpollination increase in calcium in the pistil are the walls of the transmitting cells and the intercellular matrix and, probably, additional Ca2+ uptake, initiated by pollination.
EN
The purpose of this study was to determine concentrations of serum glucose, aspartate aminotransferase (AST), insulin, beta-hydroxybutyrate (BOH) and ionised calcium (lea) in dairy cows with left displaced abomasum (LDA) and to compare these parameters before and after surgical correction of LDA. Eighteen Swiss-Holstein dairy cows with LDA were used in this study. Clinically healthy post parturient cows (n: 10) from a local dairy farm were used as control group. Blood samples were collected from the jugular vein from all the cows. Surgery was performed in cows with LDA. Blood samples from cows with LDA were collected 24 hours after surgery. The abomasum was repositioned followed by an omentopexy. Six of the 18 cows with LDA had clinical ketosis as detected with urine dipstick. The mean concentrations of insulin, BOH, glucose and AST in cows with LDA at admission time were increased compared with the healthy cows. But the mean concentration of ICa at admission time was slightly decreased compared with healthy cows. The mean BOH concentration was decreased 24 hours following surgery compared with values on admission time. However, the mean serum AST levels were increased both at admission time and 24 hours after surgery compared with healthy cows. The levels of blood gas parameters in cows with LDA were not significantly different in comparison with healthy cows, although hyperbasemia in six of 18 cows with LDA was determined. In conclusion, the results of this study indicated that serum insulin, glucose, AST and BOH levels were increased in dairy cows with LDA. Serum BOH and ICa levels were decreased 24 hours after surgery compared with values on admission time. All cows with LDA used in this study had subclini- cal/clinical ketosis. We could say that ketosis might be a risk factor for the displacement of the abomasum.
PL
Badano stan odżywienia kobiet stosujących „dietę optymalną" (dr Kwaśniewskiego). Jako mierniki stanu odżywienia zastosowano poziomy hemoglobiny, hematokrytu, glukozy, białka, cholesterolu (całkowitego, H DL, LDL), trój glicerydów, magnezu, wapnia, GGT,ALT, ALP, AM S we krwi lub osoczu. Stwierdzono prawidłowy stan odżywienia żelazem, magnezem i białkiem. Niski poziom wapnia oraz podwyższony poziom cholesterolu w osoczu krwi wskazują na niezbilansowany charakter „diety optymalnej"
EN
The nutritional status of women following optimal diet" (by dr Kwaśniewski) was assessed. The blood or serum levels of hemoglobine, hematocrite, glucose, proteins, cholesterol (total, HDL, LDL), triacylglicerides, magnesium, calcium, GGT, ALT, ALP, AMS were examined. The satisfactory nutritional status of iron, magnesium and proteins has been shown. The low calcium serum level and elevated total cholesterol one indicates, that optimal diet is not realy optimal.
PL
Badania przeprowadzono w surowicy krwi uzyskanej z Regionalnego Centrum Krwiodawstwa w Szczecinie. Uwzględniając płeć i wiek dawców podzielono na następujące grupy: K1 - kobiety w wieku od 19 do 25 lat, K2 - kobiety w wieku od 26 do 43 lat, M, - mężczyźni w wieku od 19 do 25 lat, M2 - mężczyźni w wieku od 26 do 43 lat. W materiale badawczym oznaczono stężenie magnezu, wapnia oraz triglicerydów. W opracowaniu statystycznym wyników określano wpływ magnezu i wapnia na poziom triglicerydów w surowicy krwi badanych osób. Na podstawie uzyskanych wartości współczynników korelacji Pearsona (r) zależność istotną przy umiarkowanej korelacji stwierdzono jedynie między poziomem magnezu a triglicerydów w grupie mężczyzn starszych (M2).
EN
Human blood serum received from the Regional Blood Donation Centre in Szczecin was analysed. Donors were divided into four groups according to the sex and age: K1 (women aged 19 - 25), K2 (women aged 26 - 43), Ml (men aged 19 - 25), M2 (men aged 26 - 43). Magnesium, calcium and triglycerides concentrations in the blood serum of donors were determined. Statistical influence of Mg and Ca on the triglycerides level was assessed. The values of Pearson's correlation coefficients (r) obtained indicated only one statistically significant relationship (at moderate correlation) occurring in M2 group (men aged 26 - 43).
PL
W pracy oceniono wybrane cechy tekstury i inne parametry jakościowe margaryny miękkiej wzbogaconej w preparat wapnia wyizolowany z mleka krowiego. Badano cechy tekstury metodami instrumentalnymi i sensorycznym oraz ogólną jakość sensorycznej liczbę kwasową i nadtlenkową. Obserwacje prowadzono w czasie 3 mies. przechowywania. Stwierdzono niewielki wpływ zastosowanego wzbogacania na badane cechy jakościowe. Dzienna porcja wzbogaconej margaryny (ok. 30 g) może dostarczyć ok. 80 mg wapnia, tj. 10% zalecanego spożycia, co może wpłynąć na zwiększenie poziomu wapnia w diecie.
EN
The aim of the work was to evaluate selected texture parameters and other selected quality attributes of soft margarine enriched with calcium preparation isolated form cow milk. The texture parameters were measured using instrumental and sensory methods. Overall sensory quality and chemical indicators i.e. peroxide value and acid number were estimated. All measurements were carried out during 3 months of storage. There was not significant influence of such enrichment on samples quality. The average daily portion of enriched margarine (ca. 30 g) might provide ca. 80 mg of calcium i.e. 10% of recommended daily intake, which can influence an increase of dietary calcium intake level.
PL
W badaniach doświadczalnych na szczurach określono wpływ wody demonizowanej, stosowanej per os, na poziom sodu, potasu, wapnia i magnezu we krwi i w moczu tych zwierząt.
EN
Deionized water orally dosed, ad libitum dosage were tested on rats for 90 days period. The purpose of the experiment was the estimation of changes in aqua-electrolytic economy of tested animals. Statistically relevant reduction of sodium, potassium, magnesium level in blood serum as well as in urine. In blood serum there were observed also statistically increase of calcium concentration whereas in urine calcium concentration descended. The results prove that drinking deionized water can cause similar unfavorable influence on mineral economy in human system.
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