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EN
The purpose of this experiment was to estimate the most effective doses of TFX, used in injection and in bath, for the stimulation of the activity of lymphocytes in common carp The study was performed on 9-month-old carps obtained after spring catches (in March). The fish were divided into 10 groups. Fish in groups I-IV were given TFX by intramuscular injection at doses of 20, 10, 0.5, and 0.2 mg/fish. Fish in groups VI-IX were bathed for 24, 48, and 96 hours in TFX at concentrations of 200, 100, 50, and 20 mg/l of water. Groups V and X were control. Blood samples were taken immediately after the administration of TFX in bath and after 2, 7, 14, and 28 days in groups I-IV and VIII-IX. The following parameters were determined: the number of leukocytes and lymphocytes in 1 μl of blood, the percentage of ANAE+ and E-rosette-forming cells, and the proliferative activity of lymphocyte cell cultures stimulated with Con A. Results: TFX at doses of 100, 200 mg/l of water and 10, 20 mg/fish administered by injection showed a suppressive effect on the parameters investigated. TFX at doses of 50, 20 mg/l of water and 0.5, 0.2 mg/fish caused an increase in the number of leukocytes and lymphocytes, a higher percentage of ANAE+ cells and rosette-forming cells, as well as a multiple increase in the proliferative activity of lymphocytes stimulated with Con A. The highest values were obtained after the administration of TFX in a 96-hour bath at a concentration of 50 mg/l of water.
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tom 93
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nr 02
EN
Prostaglandin F2α and its synthetic analogues, because of their luteolytic properties, have found wide application in the reproduction of domestic animals, including cattle, swine and horses. They are used to induce and synchronize heat, ovulation and parturition, as well as to improve the results of insemination and early-stage pregnancy termination. The ongoing discussions regarding animal welfare include the question of the lowest effective dose of hormones in individual species and how it could be reduced by selecting the optimal route of hormone administration. Intramuscular injection is the recommended method of administering prostaglandins to cows, pigs and mares. Due to the different sensitivities of these species to prostaglandins, the recommended therapeutic dose varies significantly from one species to another. The luteolytic effect also depends on the route of administration. Studies on alternative routes of injection report good therapeutic effects after administration of the hormone subcutaneously (SC), into intravaginal submucosa (IVSM), at the BAI-HUI acupuncture point or at the ischiorectal fossa (IRF). The injection site, the dose and the type of prostaglandin used may intensify negative effects, such as an increase in the heart or breathing rate and increased sweating.
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