Wyniki wyszukiwania - Biblioteka Nauki

1

Produkty syntezy mikrobiologicznej w paszy dla drobiu

100%

Konieczna L.

Biuletyn Informacyjny Drobiarstwa

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1993

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tom 31

|

nr 2

8-14

2

Determination of penicillin antibiotics in poultry muscle by capillary electrophoresis

63%

Kowalski P. , Konieczna L.

Bulletin of the Veterinary Institute in Puławy

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2007

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tom 51

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nr 4

3

Ekspozycja na dym tytoniowy a poziom 1-hydroksypirenu u osob palacych i niepalacych

63%

Konieczna L. , Plenis A.

Bromatologia i Chemia Toksykologiczna

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2009

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tom 42

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nr 3

553-557

4

Opracowanie metody oznaczania zawartosci azotanow [III] i [V] w przetworach miesnych z zastosowaniem kapilarnej elektroforezy strefowej

63%

Oledzka I. , Konieczna L.

Bromatologia i Chemia Toksykologiczna

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2009

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tom 42

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nr 3

503-507

5

Spectrophotometric titration of the mixtures containing antihypertensive agents in non-aqueous differentiating solvents

51%

Sell E. , Chmielewska A. , Konieczna L.

Chemia Analityczna

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2000

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tom Vol. 45, No. 2

249-255

EN

A simple, rapid, accurate and precise method for the analysis of binary mixtures: clonidine hydrochloride-hydrochlorothiazide, clonidine hydrochloride-furosemide, di-hydralazine sulfate-hydrochlorothiazide was elaborated, based on spectrophotometric titration in non-aqueous differentiating solvents. Titrations were performed with 2.5x10(-3) mol 1(-1) or 4x 10-3 mol 1(-1) solution of CH3ONa in benzene. The solvents differentiating the strength of the estimated acids were applied. The best results for the determination of the mixtures were obtained in dimethylformamide and isopropyl alcohol. The proposed method enables to determine directly the both acidic components of the mixtures, without their preliminary separation.

PL

Opracowano szybką, dokładną i prostą w wykonaniu metodę oznaczania mieszaniny chlorowodorku klonidyny z hydrochlorotiazydem, chlorowodorku klonidyny z furo-semidem oraz siarczanu dihydralazyny z hydrochlorotiazydem przy zastosowaniu spektro-fotometrycznego miareczkowania w środowisku niewodnym. Oznaczenia wykonano przy użyciu 2.5x10(-3) mol (-1) lub 4x10(-3) mol 1(-1) roztworu CH(3)ONa w benzenie, stosując rozpuszczalniki różnicujące moc oznaczanych kwasów. Najlepsze wyniki oznaczeń badanych mieszanin uzyskano w środowisku dimetyloformamidu i alkoholu izopro-pylowego. Opracowana metoda pozwala na bezpośrednie oznaczenie obydwu kwasowych składników mieszanin bez konieczności uprzedniego rozdzielania.

6

Development of a reversed-phase HPLC method for analysis of fluocinolone acetonide in gel and ointment

51%

Chmielewska A. , Konieczna L. , Lamparczyk H.

Acta Chromatographica

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2006

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tom No. 16

80-91

EN

An accurate and precise HPLC assay has been established for simul-taneous determination of fluocinolone acetonide and additives in gel. Drugs were chromatographed on a C18 reversed-phase column with 55:45 (v/v) methanol–water as mobile phase and detection at 238 nm. Solution con-centrations were measured on a weight basis to avoid the use of an internal standard. The method was statistically validated for linearity, accuracy, precision, and selectivity. Linearity for assay of fluocinolone acetonide, methyl 4-hydroxybenzoate (nipagin M), and propyl 4-hydroxybenzoate (nipagin P) were confirmed over the ranges 0.5-30, 5-200, and 10-120 µg mL-1, respectively. Because of the simplicity and accuracy of the method, it was used for routine analysis of fluocinolone acetonide in ointment. It does not require any specific sample preparation.

7

Quantitative determination of pentoxifylline in human plasma

51%

Chmielewska A. , Konieczna L. , Plenis A. , Lamparczyk H.

Acta Chromatographica

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2006

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tom No. 16

70-79

EN

A rapid, optimized, and sensitive reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been developed for deter-mination of pentoxifylline in human plasma. The analyte was extracted from the plasma with dichloromethane after addition of 0.2 mL 1 M NaOH. HPLC separation was performed on a C18 analytical column (250 mm × 4 mm i.d.) with acetonitrile–water, 45:55 (v/v), as mobile phase. Spectro-photometric detection was performed at 275 nm. Calibration graphs were linear from 25 to 1000 ng mL-1 pentoxifylline. Recovery of the drug from human plasma was 92.1%, and the detection limit was 20 ng mL-1.

8

Oznaczanie enrofloksacyny w osoczu krwi zwierzęcej metodą RP-HPLC z detekcją fluorymetryczną

51%

Chmielewska A. , Konieczna L. , Lamparczyk H.

Bromatologia i Chemia Toksykologiczna

|

2011

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tom 44

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nr 3

9

Rapid analysis of loratadine in human serum by high-performance liquid chromatography with fluorescence detection

51%

Plenis A. , Konieczna L. , Olędzka I. , Kowalski P.

Acta Chromatographica

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2010

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tom Vol. 22, no. 1

69--79

EN

A simple and rapid high-performance liquid chromatographic method with fluorescence detection for analysis of loratadine (LOR) in small volumes of human serum has been developed and validated. After solid-phase extraction (SPE), with thioridazine hydrochloride as internal standard, chromatographic separation was performed on a C 18 analytical column with 70:30 ( v / v ) acetonitrile-water, adjusted to pH 2.7 with orthophosphoric acid as mobile phase at a flow-rate of 1 min mL -1. The column was maintained at 28°C. Fluorescence detection was performed at excitation and emission wavelengths of 265 of 454 nm, respectively. The method was validated for accuracy, precision, selectivity, linearity, recovery, and stability. Absolute recovery of LOR was >93.0%. The limits of detection (LOD) and quantification (LOQ) were 0.07 and 0.2 ng mL -1,respectively. Linearity was confirmed in the range 0.2–30 ng mL -1 (correlation coefficient >0.9998). This HPLC method is selective, robust, and specific and would enable efficient analysis of large numbers of serum samples in support of pharmacokinetic, bioavailability, or bioequivalence studies after therapeutic doses of LOR.