Nowa wersja platformy, zawierająca wyłącznie zasoby pełnotekstowe, jest już dostępna.
Przejdź na https://bibliotekanauki.pl
Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 10

Liczba wyników na stronie
first rewind previous Strona / 1 next fast forward last
Wyniki wyszukiwania
Wyszukiwano:
w słowach kluczowych:  protoplast fusion
help Sortuj według:

help Ogranicz wyniki do:
first rewind previous Strona / 1 next fast forward last
1
Content available remote Enhancing gamma-decalactone production by protoplast fusion of Yarrowia lipolytica
100%
EN
γ-Decalactone is an aroma compound with a pleasant peachy odour, it can be produced by Yarrowia lipolytica, but the yield is commonly poor. Therefore, the present study aimed to use protoplast fusion performed between different superior mutants of Y. lipolytica to improve γ-decalactone production. Data showed that 45 fusants obtained from all three crosses, the cross 1 enhanced the lipase relative production (C/G) up to 106.24% for the fusant C1/9 over the original strain. Furthermore, the highest record of the lipase production was 9.50% based on the lipase relative production (C/G), with 227.82% more than the original strain, which obtained from the fusant No. C2/3 (cross2). Moreover, the highest record of the lipase production was 10 based on the lipase relative production (C/G), with 239.81% more than the wild type strain, which obtained from the fusants C3/2 and C3/14 (cross3). Finally, the highest record of the γ-decalactone production was 108.78 mg/L with 618.07% more than the wild type strain, which obtained from the fusant C3/14.
EN
Intraspecific somatic hybrids between 16 different diploid breeding lines of Solanum tuberosum L. were produced by PEG-induced fusion. Manually selected heterokaryons were cultured in a Millicells-CM using a post-fusion protoplast mixture. Plants were regenerated from calli derived from heterokaryons obtained from 10 out of 38 combinations of diploid lines. Of the tested putative somatic hybrids, 14.2% were diploid, 72.8% were tetraploid and 13% pentaploid. The DNA amplification pattern obtained with RAPD or semi-random primers confirmed that 6 fusion combinations were hybrids. In most cases, the morphological traits were intermediate to those of the diploid fusion partners. About 23.0% of the tested somatic hybrids showed variation in their morphology. Of the tested somatic hybrids, 78.0% flowered and 86.0% tuberized. The cytoplasm of 9 diploid lines and 6 somatic hybrid combinations was analysed. Two of the diploid lines had W/S chloroplasts and α or ε mitochondria; the remainder contained T chloroplasts and β mitochondria. All the analysed somatic hybrids carried T chloroplasts and β mitochondria.
EN
Genomes and their function in newly created brewery yeast hybrids are still an area of intensive and pioneering research. The stability of the resulting hybridization genom is a key element for their further functioning. This feature depends not only on de novo hybrids creation technology, but also on environmental conditions in which they grow and develop.
PL
Genomy i ich funkcjonowanie w nowo utworzonych krzyżówkach drożdży browarniczych stanowią wciąż obszar intensywnych i pionierskich badań. Stabilność utworzonego w efekcie hybrydyzacji genomu jest kluczowym elementem dla dalszego funkcjonowania krzyżówek drożdży. Cecha ta zależna jest nie tylko od techniki pozyskiwania krzyżówek de novo, ale również od warunków środowiska, w jakich następuje ich dalszy wzrost i rozwój.
first rewind previous Strona / 1 next fast forward last
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.