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EN
Protein digestibility may be Influenced by the presence of dietary fibre affecting the nutritional quality of a feed or food product. This study investigated the interplay between rapeseed (Brassica napus L.) protein and fibre constituents separated by industrially scalable pilot plant processing and recombined in mixed samples. Total dietary fibre (TDF) fractions were isolated from rapeseed hulls (TDF-RH) and purifi ed rapeseed embryo fibres (TDF-RE). The effect of TDF sources on in vitro protein digestibility (IVPD) of a rapeseed protein concentrate rich in napin proteins (RP2) was assessed at three inclusion levels (200, 333, and 500 mg/g DM) using a sequential transient proteolysis by pepsin (1 h) and pancreatin (1 h). The IVPD of RP2 was dose-dependently decreased upon addition of hull fibres at all inclusion levels (8.9-26.6%; P<0.05), whereas the effect of embryo fibres was of a markedly lower magnitude and only signifi cant at the medium to high levels (7.3-8.9%; P<0.05). These results demonstrated that TDF fractions obtained from rapeseed differentially affect the protein digestibility of rapeseed napin proteins depending on the fibre source and inclusion level.
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nr 2
223-230
PL
Opracowano procedurę analityczną oznaczania rtęci w produktach spożywczych, głównie pochodzenia roślinnego, na poziomie 5 µg/kg i niższym. Obejmuje ona mineralizację mikrofalową próbki i oznaczenie zawartości rtęci techniką CVAAS z zastosowaniem całkowicie zautomatyzowanego analizatora rtęci f-my LDC, USA.
EN
The studies on the analytical procedure for mercury determination in food of plant origin on the level of 5 µ/kg, were carried out. For this reason, microwave digestion with HNO3 and cold vapour atomic absorption spectrometry (CVAAS) with tin (II) chloride reduction, were used. Both digestion and determination conditions were experimentally optimized. The procedure was checked using two real food samples: apple juice and preserved carrot with peas both spiked on two levels with mercury. On these results basic statistic parameters that charcterized the method as standard deviation, relative standard deviation and recovery, were calculated. The precision was generally better than 10%, and recovery ranged from 96 to 100%. The detection limit of the entire procedure, defined as the mercury concentration corresponding to three times the standard deviation of ten consecutive determinations of blank measurement, was 0,012 µg/l. Taking into account the amount of a sample and the factor of dilution the detection limit corresponds to 0,3 µg/kg for 1,0 g, 1,0 µg/kg for 0,3 g, and 0,15 µg/l for 2 ml of a sample. Accuracy of the analytical procedure was tested by analysing five certified reference plant materials with the mercury contents ranged from 6 to 280 µg/kg. The obtained results were in a good agreement with their certified values. Both analyses of the spiked real samples and certified reference materials allow to suggest that this analytical procedure based on microwave digestion and cold vapour determination using Fully Automated Mercury System is suitable for mercury determination in food products of plant origin on the level of µg/kg or µg/І, with a good precision and accuracy.
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nr 4
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tom 32
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nr 3
279-284
PL
W pracy przedstawiono wyniki badania zawartości alternariolu i eteru metylowego alternariolu w surowcach i przetworach owocowych, warzywnych i zbożowych. Analizy wykonano metodą chromatografii cienkowarstwowej (TLC) i wysokociśnieniowej chromatografii cieczowej (HPLC). Stwierdzono występowanie alternariotoksyn w zapleśniałych surowcach i śladowe ich ilości w niektórych przetworach.
EN
The aim of this study was to assess Altemaria mycotoxin contamination in some raw and processed plant products. Samples were analyzed after extraction with ethyl acetate and purification on silica gel or SPE C18 columns. The final extracts on silica gel plates were determined by two-dimensional thin layer liquid chromatography and reverse phase С18 high performance liquid chromatography with UV detection. The average recoveries were 72% to 81% at 2-10 mg/kg detection limit. As much as 309 samples were analyzed: 118 raw samples (including 35 mouldy fruit and 28 low quality cereals) and 191 processed plant products. The contamination was found to be present only in evidently rotten and low quality samples. The determined levels of alternariol (AOH) ranged from 3 to 420 mg/kg, and of alternariol methyl ether (AME) from 10 to 100 µg/kg. Trace amounts of AOH were detected in 4 samples of the processed products.
EN
We investigated food preferences of three species of dormice, testing consumption by captive animals. One type of food was provided to an animal and its “suitability” scored for each species, according to whether it was or was not consumed. If it was eaten, the time when it was consumed was recorded (after 24, 48 or 72 hours). In total, 17 types of animal food and 46 of plant food were tested. Animal foods offered included different arthropods, eggs, snails and chicken meat. Plant food consisted of fruits, seeds, nuts and green parts. Glis glis consumed – 24% of animal material offered and 100% of plant food types, Dryomys nitedula consumed – 77% of animal and 54% of plant food types. Graphiurus murinus consumed – 94% of animal and 63% of plant food types. G. glis showed a significantly higher preference for plant foods rather than animal material, and preferred plant food much more than the other two species. Both D. nitedula and G. murinus preferred animal more than plant food and did not differ in preferences and diversity of both kinds of food they consumed. Thus, G. glis can be considered as principally a herbivore, while D. nitedula and G. murinus are rather meat-eaters. It can be concluded that food niches, especially of the two European dormice, are separate and thus in natural conditions they do not compete strongly for food resources.
PL
Oznaczono za pomocą spektrofotometrii absorpcji atomowej zawartość metali ciężkich (Pb, Cd, Zn i Cu) w ziemniakach, warzywach i owocach, pochodzących z rejonu oddziaływania Huty Miedzi „Głogów". Zawartość tych pierwiastków w ziemniakach, marchwi, kapuście, burakach i jabłkach stanowiła 10-50% maksymalnych dopuszczalnych wartości, obowiązujących w okresie badań. W ponad 50% próbek naci pietruszki i w 10% próbek sałaty stwierdzono ponadnormatywne ilości ołowiu.
EN
Lead, cadmium, zinc and copper contents of potatoes, carrot, cabbage, beetroot and apples collected in 1995-1997 from the emission region of the "Głogów" copper foundry were determined by atomic absorption spectrometry (AAS). Mean lead and cadmium contents of potatoes, carrot, cabbage, beetroot and apples were similar, ranging from 0.020 mg/kg to 0.063 mg/kg and from 0.005 mg/kg to 0.016 mg/kg, respectively. These values represented from 10% to 50% of the maximum values admissible at the time of the study. Mean lead content in the leaves of parsley and lettuce was 0.805 mg/kg and 0.241 mg/kg, respectively. Maximum admissible value for lead was exceeded in 50% examined parsley leave and 10% lettuce samples. Zinc and copper levels in all studied products, except for lettuce, were similar and ranged from 1.52 to 8.60 mg Zn/kg and 0.30 to 3.94 mg Cu/kg. Copper content of all lettuce samples collected in 1997 was from 105% to 195% maximum values admissible at the time of the study.
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nr 4
329-334
PL
W pracy oceniano potencjał anty oksydacyjny kwercetyny wykorzystując w tym celu metodę Total Antioxidant Status (TAS) i Peroxyl Radical Trapping Capacity (PRTC).
EN
The aim of the study was to estimate antioxidant potential of quercetin, a plant component of food products. Two analytical methods, Total Antioxidant Status (TAS) and Peroxyl Radical Trapping Capacity (PRTC) were used. Quercetin was shown to be an antioxidant. Its antioxidant potential is however, much lower than that of vitamin C, but considerably higher than that of Trolox (a water-soluble vitamin E analogue). The antioxidant properties of quercetin seem to depend on its chemical structure and is associated with OH groups found in the molecule.
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