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1
Content available remote Clinical Spectrum of Malrotation in Children
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EN
The aim of the study. Retrospective analysis of diagnosis, surgical management and final outcome of malrotation in children.Material and methods. Clinical charts, operative notes and imaging studies of all children operated on for malrotation in years 1985-2005 were studied. Children in whom malrotation was diagnosed incidentally during an operation performed because of other indications were excluded. A detailed analysis of the presentation, signs and symptoms, clinical course, and diagnostic modalities used in affected patients was carried out. Anatomical variants of malrotation and their operative management were evaluated as well.Results. In the period from 1985 to 2005 forty children with malrotation were treated. There were 25 neonates, 10 infants and 5 children older than 1 year of age. Thirty-five children were referred directly as inpatients from other hospitals. An acute or urgent clinical presentation was noted in 32 children, while in 8 patients as the presentation was subacute or chronic. In all children bile-stained vomiting was the predominant sign. In 21 children, ileus was the referral diagnosis. Only 5 children were admitted with an established diagnosis of malrotation. In six cases duodenal atresia was suspected. Delay in diagnosis of congential intestinal anomaly was identified in 15 children. In five newborns, the initial diagnosis was enterocolitis. Four other patients, including one infant and three older children, were admitted to the pediatric departments several times before receiving the final diagnosis. In 18 children, the only pre-operative imaging was abdominal plain X-ray. In 22 patients, contrast studies were performed as well. All children underwent operative management. Ladd's syndrome was diagnosed in 16 patients, and in another 7 children was associated with small bowel torsion. Isolated small bowel torsion was noted in 7 patients. In 6 patients, midgut volvulus with strangulation was found intraoperatively. Two children with typical anatomical features of non-rotation presented with recurrent episodes of ileus. In single cases, mesocolic internal hernia and sigmoid torsion were noted. 5 children died postoperatively, four of whom had midgut volvulus with massive small bowel necrosis.Conclusions. Malrotation is a rare congenital anomaly that can present with a wide anatomical and clinical spectrum but should be considered in the differential diagnosis for all children, regardless of age, manifesting with bile-stained vomiting. Suspicion of malrotation seems to be an obvious indication for referral to a tertiary pediatric surgical department where necessary imaging should be undertaken with the participation of an experienced radiologist. Regardless of its clinical presentation, malrotation should be considered an unequivocal indication for surgical treatment due to risk of midgut volvulus with its serious sequelae.
3
Content available remote A Midgut Malrotation with Entire Small Bowel Necrosis
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EN
A case of previously undiagnosed midgut malrotation with bowel torsion and subsequent total small bowel necrosis in adult is described. Curative resection saved patient's life. In short discussion, diagnostic and management pitfalls of late clinical presentation of midgut malrotation are described.
EN
Essential polyunsaturated fatty acids (PUFAs) in the feed may affect the gastrointestinal microbiota. The present study investigated the effect of 35-day supplementation of mice diet with 5% concentration of high-ω-3 PUFAs in flaxseed with focus on intestinal metabolism of mice. Capillary isotachophoresis method was used for the assessment of the level of organic acids in the gut material and faeces. Supplementation of flaxseed increased the level of organic acids in the caecum (acetic, propionic, butyric and valeric acids) and faeces (lactic, acetic, butyric acids). The most significant effect was observed on day 28 of flaxseed supplementation. The investigated additive had a stimulatory effect on intestinal metabolism and fermentation activity of beneficial bacteria.
EN
Ergosan contains 1% alginic acid extracted from two brown sea weeds. Little is known about the target organs and anatomical distribution of Ergosan (alginic acid) in fish. Therefore, feasibility of developing alginic acid nanoparticles to detect target organ in rainbow trout is interesting. To make nanoparticles, Ergosan extract (alginic acid) was irradiated at 30 kGy in a cobalt-60 irradiator and characterized by transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FTIR). Results from TEM images showed that particle sizes of irradiated alginic acid ranged from 30 to 70 nm. The FTIR results indicated that gamma irradiation had no significant influence on the basic structure of alginic acid. Later, alginic acid nanoparticles were successively labelled with 67Ga-gallium chloride. The biodistribution of irradiated Ergosan in normal rainbow trout showed highest uptake in intestine and kidney and then in liver and kidney at 4- and 24-h post injection, respectively. Single-photon emission computed tomography (SPECT) images also demonstrated target specific binding of the tracer at 4- and 24-h post injection. In conclusion, the feed supplemented with alginic acid nanoparticles enhanced SPECT images of gastrointestinal morphology and immunity system in normal rainbow trout.
EN
Ergosan contains 1% alginic acid extracted from two brown sea weeds. Little is known about the target organs and anatomical distribution of Ergosan (alginic acid) in fi sh. Therefore, feasibility of developing alginic acid nanoparticles to detect target organ in rainbow trout is interesting. To make nanoparticles, Ergosan extract (alginic acid) was irradiated at 30 kGy in a cobalt-60 irradiator and characterized by transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FTIR). Results from TEM images showed that particle sizes of irradiated alginic acid ranged from 30 to 70 nm. The FTIR results indicated that gamma irradiation had no signifi cant infl uence on the basic structure of alginic acid. Later, alginic acid nanoparticles were successively labelled with 67Ga-gallium chloride. The biodistribution of irradiated Ergosan in normal rainbow trout showed highest uptake in intestine and kidney and then in liver and kidney at 4- and 24-h post injection, respectively. Single-photon emission computed tomography (SPECT) images also demonstrated target specifi c binding of the tracer at 4- and 24-h post injection. In conclusion, the feed supplemented with alginic acid nanoparticles enhanced SPECT images of gastrointestinal morphology and immunity system in normal rainbow trout.
EN
Background. The ability of lactobacilli to adhere to the surface of the intestine is an important functional characteristic which can largely determine the effective colonization of the intestinal tract by probiotics. The following study compares the adhesion efficiency of the twenty strains of Lactobacillus genus belonging to Casei group to the Caco-2 cells and gastrointestinal mucus. Material and methods. Twenty isolates of lactobacilli belonging to Casei group were tested. The ability of bacterial cells to adhere to mucus was examined using adhesion assay to gastrointestinal mucus. Obtained results were compared with adhesion efficiency to Caco-2 cells. Phylogenetic relationship between isolates was analysed by rep-PCR. Results. The results showed large differences in adhesion efficiency between strains, as well as differences in the efficiency of adhesion to the intestinal epithelial cells and mucus. Group similarity highlighted by a rep- PCR technique does not correspond with groups of similarity in terms of the characteristics of the ability to adhere to mucus or the epithelial cells of intestinal tract. Conclusions. Strains having a high adhesion efficiency to enterocytes do not always show a high adhesion efficiency to the mucus. This may indicate the presence of different and multiple factors responsible for adhesion efficiency of Lactobacillus group Casei strains to epithelial cells and mucus.
PL
Zdolność bakterii Lactobacillus z grupy Case do przylegania do powierzchni jelit jest istotną cechą funkcjonalną, która w znacznym stopniu może decydować o skuteczności probiotyku. W proces adhezji są zaangażowane liczne czynniki obecne na powierzchni komórek bakteryjnych oraz nabłonka jelitowego. Złożony charakter zjawiska adhezji sprawia, że dokładne poznanie jego mechanizmów jest trudne i wymaga licznych badań. W pracy porównano wydajność adhezji dwudziestu szczepów bakterii Lactobacillus z grupy Casei do komórek Caco-2 oraz do śluzu przewodu pokarmowego. Uzyskane wyniki wykazały duże zróżnicowanie wydajności adhezji między poszczególnymi szczepami, jak również różnice w wydajności adhezji bakterii do komórek nabłonkowych jelit i śluzu.
PL
Rozwojowi przedwczesnych chorób układu krążenia, takich jak zawał mięśnia sercowego, często towarzyszą zaburzenia w metabolizmie lipidów, szczególnie hipertrójglicerydemia i dyslipoproteinemia. Praca ta zaznajamia ze stosowaną nomenklaturą i omawia kolejne etapy w metabolizmie lipidów i lipoprotein w zdrowiu i chorobie.
EN
Fish of five species of Notothenioidei (104 specimens), Cottoperca trigloides, Patagonotothen brevicauda, P. longipes, P. tessellata and Champsocephalus esox, caught in the Beagle Channel (Magellanic sub-region, sub-Antarctica) were infected with Digenea of nine species (1130 specimens). Faunistic data on the occurrence of all nine parasites are provided. The most abundant digenean species was Macvicaria magellanica found in the intestine of three host species of the genus Patagonotothen. The second most abundant digenean species was Elytrophalloides oatesi found in the stomach of four host species, with exception of P. brevicauda. Three digenean species: Stenakron kerguelense, Whitegonimus ozoufae and Genolinea bowersi, were more abundant in fish caught at the harbor of Ushuaia (depth 7–9 m), remaining six species: M. magellanica, Neolepidapedoides subantarcticus, Postmonorchis variabilis, Derogenes varicus, E. oatesi and Lecithaster macrocotyle, in the eastern mouth of the Beagle Channel (depth 30 m).
EN
We investigated wolf Canis lupus Linnaeus, 1758 food habits in central Italy by examining stomach and/or intestine contents of 59 individuals. Road accident and illegal kills were main causes of the wolves' death. Ungulates represented the bulk of the diet (55% in frequency), and among them wild boar was the most important prey, followed by domestic Caprinae. Food items of domestic origin accounted for about 1/3 of all the diet. Diet composition did not vary between stomachs and intestines in spite of the higher degree of digestion of the intestines' contents. The frequent detection of numerous larvae of Diptera and/or necrophagous Coleoptera, let suppose the consumption of already dead animals, and suggests a general underestimate of the wolfs scavenging behaviour in previous studies based on scat analyses.
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