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EN
The aim of the research was to evaluate that the effect of symbiotic fermented cherry juice containing fructo oligosaccharide to enhance the growth and activity of probiotic strains include Lactobacillus acidophilus was tested for their antibiotic susceptibility, and tolerance to bile. Antifungal activity of symbiotic cherry juice could differ in their antagonistic activity against fungal disease which could be due to the metabolite secreted by the lactic acid bacteriocin specially type of organic acids and added fructo oligo saccharide as a probiotic and for food preservation synbiotic cherry juice was identified and their major compounds was detected using gas chromatographymass spectrum (gc-ms).
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tom 62
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nr 07
762-765
EN
The aim of study was to investigate the effect of different dietary carbohydrate sources (couch grass, FOS or yeast cell walls, MOS) on the performance of piglets from birth to 84-days-of-life, diarrhea incidences, as well as on some blood parameters. A total of 286 piglets (Polish Landrace x Pietrain) of both sexes, from twenty seven litters, were used. Piglets were weaned at 28-days-of-age. From the second-week-of-age until the 14th day after weaning, the piglets were fed a commercial Prestarter mixture ad libitum, and from 42th to 84th-day-of-age they were fed a Starter diet. The mixtures did not contain antibiotics. The piglets of group I (control) received basal diets, group II (MOS) - the same diets, supplemented with a yeast cell wall preparation (Bio-Mos) and group III (FOS) - with couch grass. The level of MOS supplementation (3 g/kg diet) and couch grass (FOS) in meal form was added at the level of a 5 g/kg diet. Body weight was measured at birth, at the weaning period and at the end of the trial (84th-day-of-life). Feed intake was recorded weekly. The health status of the piglets and their diarrhea incidences were recorded daily. The mortality of piglets from birth till weaning and up until 12-weeks-of-life were recorded as well. The piglet’s diarrhea incidences (number of piglets and periods of duration) were also noted. Blood for analytical tests was taken at weaning (28th day) and on day 56 and 84 after birth from 24 piglets (4 gilts and 4 barrows from each group). The number of red blood cells (RBC), hemoglobin, hematocrit, number of white blood cells (WBC), neutrophiles, eosinophiles, basophiles, lymphocytes and monocytes in whole blood and total cholesterol, HDL-cholesterol and triglycerides in blood plasma were determined. The MOS or FOS supplement may be beneficial in piglet rearing as it reduces the piglet losses in the whole rearing period (from birth to 84 days) from 12.24% in the control group (without AGP) to 6.32% in the group fed the mixture supplemented with MOS and to 8.25% with FOS. These additives induced advantageous performance traits and lowered the level of total cholesterol and LDL-cholesterol.
PL
Fruktany, podobnie jak błonnik, nie ulegają trawieniu w przewodzie pokarmowym ze względu na brak w soku żołądkowym, trzustkowym i jelitowym enzymów hydrolizujących wiązania β-(2®1) glikozydowe, jednakże ulegają one częściowej hydrolizie w środowisku kwaśnym. Celem pracy była hydroliza fruktanów w warunkach symulujących działanie soku żołądkowego. Hydrolizę prowadzono w temp. 36,6–37°C, w ciągu 30–180 min, przy pH = 2, a w przypadku mączki cykoriowej z użyciem pepsyny. Badaniom poddano handlowe preparaty fruktooligosacharydów: FOS Wako Pure, Raftilose oraz preparaty otrzymane w Politechnice Łódzkiej: preparat FOS-PŁ, inulinę krystaliczną z cykorii, mączkę cykoriową, nystozę oraz sacharozę. Do oznaczenia składu preparatów przed i po hydrolizie zastosowano chromatografię cieczową HPLC. Stwierdzono, że stała szybkości hydrolizy fruktanów wyznaczona wg mechanizmu reakcji jednocząsteczkowej wynosi od 0,0006 do 0,0035. Najbardziej odporne na hydrolizę, prowadzoną w warunkach symulujących działanie soku żołądkowego, były: inulina krystaliczna, której po upływie 180 min pozostało 95% początkowej ilości oraz inulina zawarta w nisko przetworzonej mączce cykoriowej (93%). Najłatwiej ulegały hydrolizie preparaty zawierające β-fruktooligosacharydy homogenne (Raftilose) oraz oligomery o DP 3-4 (FOS Wako Pure, FOS-PŁ). Najwięcej cukrów metabolizowanych (do 30%) uwalniało się podczas hydrolizy preparatów (Wako Pure i FOS-PŁ) zawierających jako główne FOS kestozę, nystozę i fruktozylonystozę.
EN
Fructans, just like cellulose, are not digested in the gastrointestinal tract. The reason is that there are no enzymes in the gastric, pancreatic and intestinal juices, which hydrolyze the b-2,1 glycoside bonds. However, they are partially hydrolyzed under the acidic conditions. The objective of this study was the hydrolysis of fructans under the conditions, which simulate the effects of gastric juice. The 30 to 180 minute lasting hydrolysis was conducted at a temperature ranging between 36.6 and 37.0oC, at ph equalling 2. In the case when chicory meal was used, pepsin was also applied. The commercial preparations of FOS (FOS Wako Pure, Raftilose) were applied for the hydrolysis, as well as preparations supplied by the Łódź University of Technology, i.e.: FOS-PŁ, crystalline inulin from chicory, chicory dry pulp, nystose, and sucrose. The composition of the preparations before and after the hydrolysis was determined using a HPLC method. It was stated that the kinetic constant of the hydrolysis of fructan, determined according to the mechanism of a one-molecule reaction, ranged from 0.0006 to 0.0035. The following inulin types showed the highest resistance towards the hydrolysis: crystalline inulin and inulin contained in the chicory meal. After a 180 minute period of the hydrolysis, the amount of crystalline inulin was 95%, and of the inulin contained in the not much processed chicory meal: 93%. Preparations containing homogenous b-fructooligosaccharides (Raftilose) and oligomers with DP equalling 3 to 4 (FOS Wako Pure, FOS-PŁ) hydrolyzed the best. The highest amounts (up to 30%) of hydrolyzed saccharides were released during the hydrolysis of the preparations Wako Pure and FOS-PŁ; in these preparations, the key FOS components were: kestose, nystose, and fructosyl-nystose) .
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