Wyniki wyszukiwania - Biblioteka Nauki

1

Development and validation of an HPTLC method for estimation of coronarin D in Hedychium coronarium rhizome

100%

Ray A. , Dash B. , Sahoo S. , Sahoo A. , Jena S. , Kar B. , Chatterjee T. , Ghosh B. , Nayak S.

Acta Chromatographica

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2017

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tom Vol. 29, no. 3

415--426

EN

Rhizome extracts of Hedychium coronarium are widely used as phytotherapeutics. As of date, there is no documented study on the standardization of H. coronarium extract, and the following research is an effort in this direction. Coronarin D is an important bioactive compound present in H. coronarium which shows chemopreventive activity against cancer. H. coronarium extracts were assessed for coronarin D content for the first time. The extraction was checked using different solvents: n-hexane, acetone, and methanol. Coronarin D was separated on silica gel 60F254 high-performance thin-layer chromatography (HPTLC) plates by isocratic gradient method using n-hexane-ethyl acetate (80:20 v/v) as mobile phase. Densitometric quantification was performed at 231 nm in absorption mode. This method gave a well-defined peak at Rf 0.20 corresponding to coronarin D. The method was validated using International Conference on Harmonization (ICH) guidelines in terms of precision, repeatability, and accuracy. Linearity range of coronarin D was 200–1000 ng spot−1 with a correlation coefficient of R2 ± SD = 0.9987 ± 2.62% in the concentration range of 200–1000 ng spot−1. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 35 and 115 ng, respectively. Accuracy of the method was checked by recovery studies conducted at three different concentration levels, and the average percentage recovery was found to be 98.22 % for coronarin D. Among the different solvents, acetone produced maximum extraction efficiency of coronarin D. The proposed HPTLC method can be applied for robust identification and quantitative determination of coronarin D in H. coronarium extracts.

2

Protection of visual data transmission for vessel traffic systems using Joint Fingerprinting and Decryption method based on modified hill cipher

100%

Czaplewski B. , Czaplewski K.

Annual of Navigation

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2012

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tom No. 19, part 2

5--17

EN

Vessel traffic systems provide a high level of safety on coastal waters due to coastal radar stations and industrial cameras transmitting information to traffic supervision centers, as well. To improve a vessel traffic services is very important to ensure the speed and secrecy for the transmission of video images. The paper presents the basic issues of the multimedia data protection by digital watermarking and fingerprinting methods. Main applications for such digital marking were described in the paper as well as its requirements. Furthermore, the importance of multicast transmission for fingerprinting methods was presented by comparing the scalability of methods using only unicast transmissions and methods using multicast transmissions. The paper also presents the greatest threat to fingerprinting methods, which are attacks performed by more than one pirate. These attacks are called collusion attacks. The criteria that should be followed during identifying rogue users taking part in the collusion attack on the security systems has been presented. The paper also contains description of the extended Hillcast method, which belongs to the group of JFD (Joint Fingerprinting and Decryption) methods. The method provides a cryptographic security and digital fingerprinting of multimedia content, while maintaining high scalability. Main purpose of this method is VoD (Video on Demand) service, but it can also be used in vessel traffic supervision systems, such as VTS and AIS. In the last part of paper, there are results of studies which indicate high resistance to most common collusion attacks. Method proposed by authors can enhance the security of visual data transmission in vessel traffic systems.

3

Random amplified polymorphic DNA fingerprinting as a marker for Paramecium jenningsi strains

100%

Skotarczak B. , Przybos E. , Wodecka B. , Maciejewska A.

Folia Biologica

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2004

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tom 52

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nr 1-2

4

Simultaneous analysis of six bioactive lignans in Phyllanthus species by reversed phase hyphenated high performance liquid chromatographic technique

88%

Shanker K. , Singh M. , Srivastava V. , Verma R. K. , Gupta A. K. , Gupta M. M.

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2011

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tom Vol. 23, no. 2

321--337

EN

An online-hyphenated high-performance liquid chromatography-photodiode array-mass spectrometry (HPLC-PDA-MS) analytical method was developed for the simultaneous determination of six lignans of therapeutic importance in four Phyllanthus spp. (P. amarus, P. maderaspatensis, P. urinaria, and P. virgatus). HPLC with monolithic reverse phase silica column (4.6 × 100 mm) and simple isocratic elution of methanol-water mixed with dioxane facilitated the separation of lignans of diverse nature such as diarylbutyrolactone, tetrahydrofuran, isomeric aryltetralin, and diarylbutane type for quantitative analysis. Targeted lignans viz. heliobuphthalmin lactone (1), virgatusin (2), hypophyllanthin (3), phyllanthin (4), nirtetralin (5), and niranthin (6) were confirmed unambiguously in four Phyllanthus species by their abundant molecular adduct ions, retention time, UV, and mass spectra as compared with those of reference compounds. Advantages and limitations of both detection techniques for qualitative (fingerprinting) and quantitative analysis of the above mentioned lignans in four Phyllanthus spp. are discussed. The method was validated following international guidelines. The described method can be utilized for assays and stability tests of P. amarus extracts as well as traditional Indian medicine based on Phyllanthus herb.

5

The kernel density estimate as a measure of the performance of one and two-dimensional TLC systems with large retention datasets in the context of their use in fingerprinting

88%

Komsta Ł. , Szewczyk K.

Acta Chromatographica

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2009

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tom Vol. 21, no. 1

13-27

EN

A new objective chromatographic response function, R K , based on the kernel density estimate, is introduced for estimation of the fingerprinting performance of a particular TLC system (uniformity of retention) for which a large set of experimental R F values of possible components of the mixture is available. The R K criterion is insensitive to large numbers (hundreds or thousands) of R F values, when the previously proposed criteria cease. It can be applied to one and two-dimensional TLC and is easily computed. As an example of its application, the performance of twelve general screening systems was evaluated in the context of herbal extract fingerprinting (88 phytochemical standards) by both one and two-dimensional TLC.

6

Simultaneous component analysis as an interesting preliminary data analysis method in GC-MS – An example of headspace screening of Polish grasses

88%

Wróbel-Szkolak J. , Cwener A. , Pietraś R. , Komsta Ł.

Acta Chromatographica

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2024

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tom Vol. 36, no. 1

52--58

EN

70 species of grasses family (Poaceae), coming from genera: Agrostis, Alopecurus, Anthoxanthum, Apera, Arrhenatherum, Avena, Brachypodium, Briza, Bromus, Calamagrostis, Corynephorus, Cynosurus, Dactylis, Danthonia, Deschampsia, Digitaria, Echinochloa, Elymus, Eragrostis, Festuca, Glyceria, Helictotrichon, Hierochloe, Holcus, Hordeum, Koeleria, Leymus, Lolium, Milium, Molinia, Nardus, Panicum, Phalaris, Phleum, Phragmites, Poa, Saccharum and Setaria, collected mostly from natural stands in Poland during 2020 season, were subjected to GC-MS fingerprinting of headspace volatile fraction above dried material. Obtained mass spectrometry data were analyzed by means of principal component analysis (PCA) and hierarchical cluster analysis (HCA). Five species: Glyceria maxima (Hartm.) Holmb., Lolium multiflorum Lam., Hordeum jubatum L., Bromus tectorum L. and Bromus secalinus L. were identified as outliers, which is consistent with our earlier analysis by thin layer chromatography. These species deserve further look and their outliance is orthogonal to coumarin content, which was independently observed for odorant species of grasses.

7

Food metabolomics in the post-genomic era: specific fingerprint and biomarkers for vegetable oils and fruit juices

75%

Socaciu C. , Parloc R. , Leopold L. , Ranga F. , Fetea F.

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tom 61

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nr Suppl.1

8

Fingerprinting of on-farm conserved local tunisian orange cultivars (Citrus sinensis (L.) Osbeck) using microsatellite markers

75%

Abdelaali S. B. , Debbabi O. S. , Abdelaali N. B. , Hajlaoui M. R. , Mars M.

Acta Biologica Cracoviensia. Series Botanica

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2018

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tom 60

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nr 1

9

Badania nad Radix Sophorae Subprostratae za pomocą techniki nieliniowych fingerprintów chemicznych (pełne spektrum)

75%

Ding F. , She Y. , Fang X. , Li S. , Liang Y. , Zhang T. , Si S.

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2018

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tom T. 97, nr 11

1884--1888

PL

Technika nieliniowego chemicznego fingerprintu NCF (non-linear chemical fingerprint) może być z powodzeniem stosowana do kompleksowej oceny układów złożonych. NCF wykorzystano do badania korzenia rośliny Sophorae Subprostratae ( Sophora subprostrata Chun et T. Chen), stosowanego w tradycyjnej medycynie chińskiej. W badaniach uwzględniono parametr jakościowy i parametr ilościowy reakcji (czas i potencjał) oraz ustalono matematyczną zależność pomiędzy nimi. W układzie oscylacyjnym złożonym z bromianu sodu, kwasu siarkowego, acetonu i siarczanu manganu optymalny zakres detekcji składników Radix Sophorae Subprostratae uzyskano dla ilości 0,7-1,5 g sproszkowanego korzenia. Wykazano pewne różnice w chemicznym składzie próbek Radix Sophorae Subprostratae pozyskanych z różnych regionów. Uzyskane wyniki porównano z wynikami analizy chromatograficznej.

EN

Radix Sophorae Subprostratae (traditional Chinese medicine) was evaluated in a fixed system consisting of NaBrO3, H2SO4, Me2CO and MnSO4 do det. its fingerprint. Three oscillation areas were established. The results agreed with chromatog. detd. data.

10

Stare i nowe metody identyfikacji odmian

75%

Orlikowska T.

Hasło Ogrodnicze

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2004

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nr 06

128-130

11

Fingerprinting of common wheat cultivars with an Alw44I-based AFLP method

75%

Tyrka M.

Journal of Applied Genetics

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2004

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tom 45

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nr 4

12

Rep-PCR fingerprinting as a tool for the analysis of genomic diversity of Escherichia coli strains isolated from a water environment

75%

Niedbach J. , Blady-Chudzik K. , Stosik M.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr Suppl.

13

Comparative analysis of anthocyanin composition of juices obtained from selected species of berry fruits

75%

Stoj A. , Malik A. , Targonski Z.

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nr 4

EN

A qualitative and quantitative analysis of anthocyanins in juices of three varieties of strawberry (Senga, Ducat, Marmolada), raspberry (Beskid, Canby, Malling Seedling), black currant (Ben Lomond, Titania, Ojebyn) and red currant (Rondom, Jonker, Holenderska) picked in three following years: 1998, 1999 and 2000, was presented in this paper. An HPLC technique was applied using a Gilson chromatograph and a DAD detector. Prior to the chromatographic analysis, anthocyanins were purified on a mini-column Sep-Pak C18 Waters. It was indicated that within species the juices examined differed in the quantitative and qualitative composition of anthocyanins. Pelargonidin-3-glucoside and cyanidin-3-xylorutinoside were the main anthocyanins in strawberry and red currant juices, respectively, independently of variety. Those anthocyanins were not detected in raspberry and black currant juices, in which cyanidin-3-sophoroside as well as delphinidin-3-rutinoside and cyanidin-3-rutinoside were the main anthocyanins, respectively. Differences of anthocyanin composition of juices obtained from different berry fruits create the possibility of detecting the adulterations of expensive raspberry and black currant juices with cheap strawberry and red currant juices on the basis of anthocyanin analysis.

PL

W pracy oznaczono skład ilościowy i jakościowy antocyjanów w sokach otrzymanych z trzech odmian truskawek (Senga, Ducat, Marmolada), malin (Beskid, Canby, Malling Seedling), czarnych porzeczek (Ben Lomond, Titania, Ojebyn) i czerwonych porzeczek (Rondom, Jonker, Holenderska) zebranych w trzech kolejnych latach: 1998, 1999 i 2000. Wykorzystano technikę HPLC używając chromatografu Gilson i detektora DAD. Przed analizą antocyjany oczyszczano na minikolumnie Sep – Pak C18 Waters. Wykazano, że badane soki w obrębie gatunków różniły się składem ilościowym i jakościowym antocyjanów. Niezależnie od odmiany, dominującymi antocyjanami w sokach truskawkowych i z czerwonych porzeczek były odpowiednio pelargonidyno-3-glukozyd i cyjanidyno-3-ksylorutynozyd. Obecności tych antocyjanów nie stwierdzono w sokach malinowych i z czarnych porzeczek, w których głównymi antocyjanami były odpowiednio cyjanidyno-3-soforozyd oraz delfinidyno-3-rutynozyd i cyjanidyno-3-rutynozyd. Przypuszcza się, że na podstawie analizy antocyjanów można wykryć zafałszowania drogich soków malinowych i z czarnych porzeczek tanimi sokami truskawkowymi i z czerwonych porzeczek.

14

Insertion of a reamplification round into the ISSR-PCR protocol gives new flax fingerprinting patterns

75%

Wiesner I. , Wiesnerova D.

Cellular and Molecular Biology Letters

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2003

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tom 08

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nr 3

EN

We expanded the basic ISSR-PCR protocol by an additional PCR reamplification round in order to detect whether increased PCR productivity would give new bands in ISSR patterns. We found that the reamplification step had a prominent impact on the quality of the inter-simple-sequence repeat (ISSR) PCR patterns of flax, depending on the particular primer used for PCR amplification. We could clearly distinguish between two types of reamplification effect. Most ISSR primers (16 out of 21) gave no reamplification effect as usual, but five primers (23.8%) provided a new ISSR fingerprinting pattern after the 2nd reamplification round, leaving the previous 1st round pattern completely blank. Therefore, we recommend the expansion of a basic ISSR-PCR protocol for another reamplification round in order to mine out full the fingerprinting potential from ISSR-PCR method.

15

An estimate of the level of polymorphism between forms of spring barley [Hordeum vulgare L.]

75%

Witkowicz J. , Strzelczyk J. , Kucharska M.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr Suppl.

16

Rep-PCR fingerprinting as a tool for the analysis of genomic diversity in Escherichia coli strains isolated from an aqueous-freshwater environment

75%

Baldy-Chudzik K. , Niedbach J. , Stosik M.

Cellular and Molecular Biology Letters

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2003

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tom 08

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nr 3

EN

The rep-PCR fingerprinting method, with the support of ERIC and REP primers, was used to analyse the genomic diversity of 93 E. coli strains isolated from lake water samples drawn at two different depths. The applied UPGMA for DNA analysis did not reveale any genomic similarities between the 48 E. coli strains derived from the subsurface-zone water and the 43 of the bottom-zone water. The considerable genomic diversity of the E. coli of the surface zone was expressed as a dendrogram in the form of 8 similarity groups comprising strains isolated from samples drawn over one month. The bottom-zone strains, which display a lesser degree of genomic diversity (5 similarity groups), showed distinct common features in their DNA fingerprints. In the similarity dendrogram for the bottom-zone, strains derived in different months of sampling were segregated into the same similarity groups. Applying REP primers in rep-PCR generates more complex fingerprints increasing the discriminatory power of the analysis, whereas the ERIC primer generates less complex fingerprint patterns, and is thus clearer to interpret.

17

Frymartynizm u ssakow

75%

Rejduch B. , Slota E.

Medycyna Weterynaryjna

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1997

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tom 53

|

nr 06

330-331

18

Multiple-locus variable number of tandem repeats fingerprinting (MLVF) and virulence factor analysis of methicillin resistant Staphylococcus aureus SCCmec type III

63%

Emaneini M. , Jabalameli L. , Iman-Eini H. , Aligholi M. , Ghasemi A. , Nakhjavani F. A. , Taherikalani M. , Khoramian B. , Asadollahi P. , Jabalameli F.

Polish Journal of Microbiology

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2011

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tom 60

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nr 4

EN

Methicillin resistant Staphylococcus aureus (MRSA), particularly strains with type III staphylococcal cassette chromosome mec (SCCmec), represent a serious human pathogen in Tehran, Iran. The disease-causing capability depends on their ability to produce a wide variety of virulent factors. The prevalence of exotoxin genes and multiple-locus variable number of tandem repeats fingerprinting (MLVF) profile among MRSA isolates, from patients in Tehran, was evaluated by PCR and Multiplex-PCR. The MLVF typing of 144 MRSA isolates with type III SCCmec produced 5 different MLVF types. Generally, 97.2% (140/144) of all the isolates were positive for at least one of the tested exotoxin genes. The most prevalent genes were hld, found in 87.5% (126/144) of the isolates followed by lukE-lukD and hla found in 72.9% (105/144) and 70.1% (101/144) of the isolates, respectively. The tst gene, belonging to MLVF types I, IV and V, was found among three of the isolates from blood and wound samples. The sea gene was detected in 58.3% (84/144) of the isolates and the sed and see genes were found in one isolate with MLVF type V. The coexistence of genes was observed in the 87.5% (126/144) of the isolates.The rate of coexistence of hld with lukE-lukD, hla with lukE-lukD and sea with lukE-lukD were 66.7% (96/144), 44.4% (64/144) and 44.4% (64/144), respectively. The present study demonstrated that MRSA strains with type III SCCmec show different MLVF patterns and exotoxin profiles.

19

Research of accuracy of RSSI fingerprint-based indoor positioning BLE system

63%

Wysocki M. , Nicpoń R. , Trzaska M. , Czapiewska A.

Przegląd Elektrotechniczny

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2022

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tom R. 98, nr 9

86--89

EN

Radio localization in indoor environment is still a challenging task due to environment volatility. In the paper are compared achieved localization accuracies for RSSI-Fingerprinting method utilizing Bluetooth Low Energy (BLE) for two different environments: large empty hall and narrow corridor. Measurements were done by 6 different smartphones of 3 different producers, which makes those measurements unique as accuracies achieved by different devices can be compared.

PL

Lokalizacja radiowa w środowisku wewnętrznym jest nadal trudnym zadaniem ze względu na zmienność środowiska. W artykule porównano uzyskane dokładności lokalizacji dla metody RSSI-Fingerprinting z wykorzystaniem technologii Bluetooth Low Energy (BLE) dla dwóch różnych środowisk: dużego pustego holu i wąskiego korytarza. Pomiary zostały wykonane przez 6 różnych smartfonów 3 różnych producentów, co czyni te pomiary wyjątkowymi, ponieważ można porównywać dokładności uzyskiwane przez różne urządzenia.

20

Znakowanie wodne sygnałów fonicznych w dziedzinie częstotliwości

63%

Markiewicz R.

Przegląd Telekomunikacyjny + Wiadomości Telekomunikacyjne

|

2016

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tom nr 6

257—260, CD

PL

W artykule przedstawiono zagadnienia znakowania wodnego sygnałów fonicznych w dziedzinie zlogarytmowanej amplitudy widma. Artykuł został przygotowany na podstawie rozprawy doktorskiej o tym samym tytule, obronionej na Politechnice Warszawskiej w grudniu 2015 r. W artykule (i w rozprawie doktorskiej) zaprezentowano trzy rodzaje znakowania wodnego sygnałów fonicznych: znakowania adnotacyjnego, wstawiania sygnatury cyfrowej do sygnałów fonicznych oraz znakowania „odciskami palca” (ang. fingerprinting).

EN

In this paper an audio watermarking technique is presented, using log-spectrum domain for watermark embedding. This paper was prepared on the basis of the author's doctoral thesis with the same title, defended at the Warsaw University of Technology in December 2015. The paper (and doctoral thesis) presents three types of audio watermarking: annotation watermarking, digital signature embedding in audio signals and fingerprinting.