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EN
Nutritional supplements play a role in promoting human protein synthesis, fitness recovery, and mental health protection. Pollen is considered a natural food with excellent nutritional value. The use of bee-gathered pollen as a nutritional supplement is now widespread around the world, and it is appreciated for its healing qualities. However, depending on the floral species and the region of origin, pollen has a wide range of nutritional value. It was found that clover pollen had the highest protein content (31.4 g/100 g DM), while the lowest content was observed in maize pollen (21.3%). Eucalyptus pollen had the highest lipid amount (9.49 g/100 g DM), while clover pollen had the lowest content (7.46 g/100 g). Phytochemical analysis showed that eucalyptus pollen occupied the highest total antioxidant activity (67.02%), followed by clover (58.25%) then maize (52.18%), whereas clover pollen had the highest phenolic content (1165 mg GAE/100 gm), compared with the other pollen varieties (949.4 to 1073 mg-GAE/100 gm). The contents of branched-chain amino acids were 29, 33.3, and 38.4 mg/g for maize, eucalyptus, and clover respectively, representing 17.87, 18.44, and 16.53% of total amino acids. The contents of total essential amino acids (EAAs) were 61.8, 73.0, and 83.5 mg/g for maize, eucalyptus, and clover pollen, respectively. Only eucalyptus pollen ultimately met the minimum requirements of EAAs for adults. In contrast, the other two types of pollen contained at least 3–4 limiting amounts of the essential amino acids leucine, lysine, phenylalanine, and valine. In conclusion, pollen is a good, affordable source of nutrients that can be utilized as beneficial dietary supplements for human health.
EN
Forage legumes are used to enhance soil fertility of the agroecosystem. Understanding their effect on the agroecosystem during their growing period is essential. The objective of this field study was to evaluate annual clovers intercropping and earthworm activity on the growth of weeds. The field experiment was carried out during the 2006–2007 growing seasons at the research farm of the Seed and Plant Improvement Institute, Karaj (Iran) involving various mixed cropping ratios of berseem clover (Trifolium alexandrinum L., B) and Persian clover (Trifolium resupinatum L., P) (B : P = 1 : 0, 3 : 1, 1 : 1, 1 : 3) with and without earthworm Pheretima sp. inoculation. Nitrogen content of plants and weeds as well as biomass was measured. Forage yield was higher in 2006 than 2007 but had similar response to cropping systems and earthworm inoculation. Total forage yield was highest in mixed cropping system (MCS). While in the first cut, the berseem sole crop (SC) tended to greatly suppress the growth of weeds, in the second cut Persian clover sole crop further suppressed weeds. The greater total crop biomass had an even higher weed suppression. Earthworm activity did not affect cut 1 but increased forage and weed biomass yield in cut 2. However in cut 1, berseem tended to greatly suppress the growth of weeds, which may be explained by the greater nitrogen accumulation in monocultures and intercrops, Persian clover in cut 2 had greater suppression on weed biomass production. Nitrogen accumulation of crops and weed increased under earthworm activity in the second cut.
PL
Paszowe rośliny motylkowe są wykorzystywane do podniesienia żyzności gleby w agrosystemie. Zrozumienie ich działania w agrosystemie w czasie wzrostu jest podstawowe. Celem niniejszej pracy była ocena międzyplonu jednorocznych koniczyn i aktywności dżdżownic na wzrost chwastów. W sezonach wegetacyjnych 2006– 2007, przeprowadzono doświadczenie polowe w gospodarstwie doświadczalnym Instytutu Ulepszania Nasion i Roślin Karaj (Iran), uwzględniające różne proporcje mieszanek Triforium alexandrinum L., B i koniczyny perskiej (T. resupinatum L., P) (B : P = 1 : 0, 3 : 1 i 1 : 3), z inokulacją dżdżownicami Pheretina sp. lub bez takiej inokulacji. Określono zawartość azotu w roślinach i w chwastach oraz ich biomasę. Plon roślin był wyższy w 2006 r. niż w 2007 r., ale reakcja na systemy uprawy i zaszczepienie dżdżownicami była podobna. Ogólny plon roślin był najwyższy w systemie mieszanej uprawy (MCS). W pierwszym pokosie T. alexandrinum (SC), wystąpiła tendencja dużego ograniczenia chwastów, a w drugim pokosie to ograniczenie było kontynuowane. Przy większej biomasie koniczyny, występowało większe ograniczenie chwastów. Aktywność dżdżownic nie wpływała na pierwszy pokos, ale zwiększała plon biomasy drugiego pokosu. Jednak w pierwszym pokosie koniczyna wykazywała tendencję dużego ograniczenia wzrostu chwastów, co może być tłumaczone większym nagromadzeniem azotu w monokulturze i międzyplonach. Koniczyna perska w drugim pokosie wykazywała większe ograniczenie wytwarzania biomasy chwastów. W drugim pokosie akumulacja azotu w roślinach i aktywność dżdżownic wzrastały.
EN
In this work, clover was shown to respond to infection with Rhizobium leguminosarum bv. trifolii by producing reactive oxygen species. Superoxide radical and hydrogen peroxide were detected in infection threads and nodule primordia. The role of reactive oxygen species in clover-Rhizobium leguminosarum bv. trifolii symbiosis is discussed
EN
It was found that red clover utilizes from 1.1 to 1.4 kg of CO2 from the atmosphere to produce 1 kg of dry mattter. This means that 1400 to 1600m3 of atmospheric air become completely purified from carbon dioxide. Red clover plants usually lose 40.9% of their crop weight during the winter. This results from organic carbon loss equal (in CO2 units) to 41.8% for physiolopical processes. In the same period however, the ash fraction, larger grows as a simple consequence of actual carbon loss. It is reasonable to assume that the plants do intake mineral compounds from the deeper, not frozen horizons of the soil also during winter.
EN
Rhizobium leguminosarum bv. trifolii (Rlt) establishes beneficial root nodule symbiosis with clover. Twenty Rlt strains differentially marked with antibiotic-resistance markers were investigated in terms of their competitiveness and plant growth promotion in mixed inoculation of clover in laboratory experiments. The results showed that the studied strains essentially differed in competition ability. These differences seem not to be dependent on bacterial multiplication in the vicinity of roots, but rather on complex physiological traits that affect competitiveness. The most remarkable result of this study is that almost half of the total number of the sampled nodules was colonized by more than one strain. The data suggest that multi-strain model of nodule colonization is common in Rhizobium-legume symbiosis and reflects the diversity of rhizobial population living in the rhizosphere.
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tom Tom 20, cz. 2
1465--1479
PL
Celem pracy była ocena wpływu syntetycznych hormonów roślinnych z grupy auksyn i cytokinin na koncentrację K, Ca i Mg oraz stosunek ilościowy K:(Ca+Mg) w biomasie nadziemnej Medicago x varia T. Martyn i Trifolium pratense L. Badania realizowano w oparciu o doświadczenie pierścieniowe prowadzone na obiekcie doświadczalnym Uniwersytetu Przyrodniczo-Humanistycznego w Siedlcach w latach 2014-2016. Czynniki doświadczenia: – gatunek rośliny: lucerna mieszańcowa (Medicago x varia T. Martyn) odmiana Kometa oraz koniczyna łąkowa (Trifolium pratense L.) odmiana Krynia, – regulator wzrostu: (K) kontrola: woda destylowana, (A) kwas indolilo-3-masłowy (IBA – syntetyczna auksyna), (C) 6-benzyloaminopuryna (BAP – syntetyczna cytokinina), (AC) kwas indolilo-3-masłowy + 6 benzyloaminopuryna (IBA + BAP – stosunek ilościowy 1:1). Każdy odrost opryskiwano dwukrotnie. Na jednorazowy oprysk zużywano 0,2 dm3 roztwotu o stężeniu regulatora 30 mg·dm-3, uzyskując całkowite zroszenie roślin w pierścieniu. Pierścienie kontrolne opryskiwano taką samą ilością wody destylowanej. Obiekty prowadzono w trzech powtórzeniach i użytkowano trzykrotnie w sezonie wegetacyjnym. W ocenie wybranych elementów wartości użytkowej roślin, wykorzystano następujące parametry: zawartość ogólna wybranych makroelementów (K, Ca, Mg) w suchej masie roślin, stosunek ilościowy makroskładników K:(Ca+Mg). Uzyskane wyniki badań opracowano statystycznie wykorzystując analizę wariancji. O istotności wpływu czynników doświadczalnych na wartość badanych cech wnioskowano na podstawie testu F Fishera-Snedecora, a wartość NIR0,05 weryfikowano testem Tukey’a. Auksyna i cytokinina stosowane oddzielnie w uprawie lucerny mieszańcowej i koniczyny łąkowej powodowały wzrost koncentracji K i Mg w suchej masie roślin. Tylko w warunkach stosowania auksyny, udział Ca wzrastał, co nadmiernie zawężało proporcję K:(Ca+Mg).
EN
The aim of the experiment was to assess the effect of auxins and cytokinins, synthetic plant hormones, on Ca, Mg, and K concentration and the K: (Ca + Mg) ratio in the above-ground biomass of Medicago x varia t. Martyn and Trifolium pratense L.The research was based on a pot experiment conducted in an experimental facility of the University of Natural Sciences and Humanities in Siedlce in the years 2014-2016. The experimental factors were as follows: – plant species: alfalfa (Medicago x varia T. Martyna) cultivar Comet; red clover (Trifolium pratense L.) variety Krynia, – growth regulators: (A) indolilo 3-butyric acid (synthetic auxin IBA); (C) 6-benzylaminopurine (BAP synthetic cytokine); (AC) 3-butyric acid indolilo + 6-benzylaminopurine (IBA + BAP in the ratio 1: 1); control treated with distilled water. During each growth cycle, the grass was treated twice. For a single application, 0.2 dm3 of spray liquid with a growth regulator concentration of 30 mg·dm-3 was used until the plants in the pots were thoroughly wet. The control pots were treated with distilled water of the same amount. The experiment was repeated three times, with three harvests during the growing season. The following parameters were determined in the assessment of selected nutritional characteristics of plants: content of selected macroelements (K, Ca, Mg) in the dry mass of plants: ratio of macroelements in the dry matter plants: K: (Ca+Mg). The results of the study were statistically processed using the analysis of variance. F Fisher's test was used to assess the significance of the influence of experimental factors on the tested properties, while the LSD values were verified by the Tukey test. Auxin and cytokinin applied separately to hybrid alfalfa and red clover increased concentration of K and Mg in plant dry matter. Auxin used on its own increased Ca content, which excessively narrowed the ratio of K:(Ca+Mg)
EN
In field and pot experiments the effect of selected pesticides on the atmospheric nitrogen fixation, number of microorganisms in soil and yield of red clover has been investigated. The results obtained indicate that crop protection preparations applied in the experiments (Funaben T seed dressing compound and Pivot 100 SL herbicide) resulted in reduction of nitrogenase activity on the active strain of Rhizobium leguminosarum bv. trifolii KGL both in pot and field experiment conditions. Moreover, the authors observed the toxic effect of the pesticides used on nodulation, root development and yield of clover. The herbicide and fungicide applied also inhibited the multiplication of the microorganisms in soil under red clover plantations in the first days upon application and, later on, stimulated their multiplication.
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