Wyniki wyszukiwania - Biblioteka Nauki

1

The correlation of structural features of mature miRNAs with their biological function

100%

Belter A. , Naskret-Barciszewska M. Z.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2014

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tom 95

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nr 3

2

Beclin-1 and its role as a target for anticancer therapy

100%

Toton E. , Lisiak N. , Sawicka P. , Rybczynska M.

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nr 4

3

Molecular structure and biological function of von Willebrand factor

100%

Bykowska E.

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nr Supplement 4

4

The role of probiotics in the organism

100%

Siuta A.

Acta Agraria et Silvestria. Series Zootechnica

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1997

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tom 35

33-45

5

Obtaining of metallic nanoparticles by plasma-erosion electrical discharges in liquid mediums for biological application

100%

Lopatko K. G. , Melnichuk M. D. , Aftandilyants Y. G. , Gonchar E. N. , Boretskij V. F. , Veklich A. N. , Zakharchenko S. N. , Tugay T. I. , Tugay A. V. , Trach V. V.

Annals of Warsaw University of Life Sciences - SGGW. Agriculture

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2013

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nr 61 Agric.Forest Eng.

EN

Obtaining of metallic nanoparticles by plasma-erosion electrical discharges in liquid mediums for biological application. The process of electrical discharge in the water of the plasma discharge channel was investigated and its diagnostics was performed. The specific features of the energy redistribution were shown, together with the physical model of the dispersed phase. The biological functionality of the metal nanoparticles and the prospects for their use in biotechnology were found.

PL

Uzyskiwanie nanocząstek metali poprzez wyładowania elektryczne w zakresie erozji plazmy w środowisku wodnym w zastosowaniach biologicznych. Przeprowadzono badania wyładowań elektrycznych w środowisku wodnym w celu uzyskania nanocząstek metali. Określono specyficzne cechy redystrybucji energii oraz przedstawiono fizyczny model fazy rozproszonej. Określono funkcjonalność biologiczną nanocząsteczek metali oraz perspektywy ich wykorzystania w biotechnologii.

6

Plant purple acid phosphatases - genes, structures and biological function

100%

Olczak M. , Morawiecka B. , Watorek W.

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nr 4

EN

The properties of plant purple acid phosphatases (PAPs), metallophosphoesterases present in some bacteria, plants and animals are reviewed. All members of this group contain a characteristic set of seven amino-acid residues involved in metal li- gation. Animal PAPs contain a binuclear metallic center composed of two irons, whereas in plant PAPs one iron ion is joined by zinc or manganese ion. Among plant PAPs two groups can be distinguished: small PAPs, monomeric proteins with molecular mass around 35 kDa, structurally close to mammalian PAPs, and large PAPs, homodimeric proteins with a single polypeptide of about 55 kDa. Large plant PAPs exhibit two types of structural organization. One type comprises enzymes with subunits bound by a disulfide bridge formed by cysteines located in the C-terminal region around position 350. In the second type no cysteines are located in this position and no disulfide bridges are formed between subunits. Differences in structural organisation are reflected in substrate preferences. Recent data reveal in plants the occurrence of metallophosphoesterases structurally different from small or large PAPs but with metal-ligating sequences characteristic for PAPs and expressing pronounced specificity towards phytate or diphosphate nucleosides and inorganic pyrophosphate.

7

The tumor supressor function of STGC3 and its reduced expression in nasopharyngeal carcinoma

84%

He X. S. , Deng M. , Yang S. , Xiao Z.-Q. , Luo Q. , He Z.-M. , Hu B. , Chen Z.-C.

Cellular and Molecular Biology Letters

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2008

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tom 13

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nr 3

339-352

EN

STGC3 is a novel candidate tumor suppressor gene that was found to be associated with nasopharyngeal carcinoma (NPC) via the cDNA cloning and RACE processes. The biological function of the STGC3 protein and its expression level in nasopharyngeal carcinoma remain unknown. This study aimed to evaluate the STGC3 protein expression level in NPC and to investigate the inhibitory function of STGC3 as a candidate tumor suppressor gene. We assessed the expression of the STGC3 protein in NPC biopsies and normal control specimens via Western blot and immunohistochemical analysis. The expression of STGC3 as induced by doxycycline (Dox) via a tetracycline (Tet)-regulated system in human nasopharyngeal carcinoma cell line CNE2 was also established, and the effect of STGC3 restoration on the biological behavior of CNE2 was observed. A reduced level of STGC3 expression (0.978 ± 0.213 versus 0.324 ± 0.185, P < 0.05) was detected in NPC versus normal nasopharyngeal tissue by Western blot assay. Immunohistochemical assays for STGC3 detected positive staining in the nuclei and cytoplasm of epithelial cells, and the positive expression rate in NPC, 8 of 21 (38%), was lower than that in normal nasopharynx samples, 16 of 22 (72%). After STGC3 expression was restored, the growth capacity and clone formation potential of CNE2 cells in soft agar were significantly suppressed, and the cell percentage in G0/G1 phase increased, while the percentage of cells entering the S and G2 phases decreased. This indicates that an abnormality in STGC3 expression is associated with nasopharyngeal carcinogenesis and that it may play an important role in controlling cell growth and regulating the cell cycle.

8

The expression of endothelin type A and B receptors in the lateral wall of the mouse cochlea

84%

Luo Y. , Tang Y. , Xia Q. , Liu J.

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tom 12

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nr 4

595-603

EN

Endothelin (ET), originally characterized as a vasoconstrictive peptide, has been found to have many different biological functions, including acting as a local hormonal regulator of pressure, fluid, ions and neurotransmitters in the inner ear. The objective of this study was to examine and quantify the mRNA expression of the endothelin type A and B receptors (ETAR and ETBR) in the strial vascularies (StV) and non-strial tissues (NSt) of the cochlear lateral wall using the real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) technique. The mouse tissue samples were harvested and RNA was extracted. RT was performed to obtain cDNA, and then the mRNA expression of each gene was measured via real-time PCR. We found that both receptor subtypes were expressed in the cochlear lateral wall, with a predominance of ETAR over ETBR. We showed that the mRNA expression of the two receptor subtypes was higher in the StV with a 1.8 times higher level of ETAR and an 8.1 times higher level of ETBR mRNAs than in the adjacent NSt of the lateral wall tissue. This study shows the existence and the quantity of ET receptor subtypes in the StV and NSt of the mouse cochlea. Our results suggest that an endothelin-mediated response via two different receptors, ETAR and ETBR, may play an important role in the physiological functions of the cochlear lateral wall by maintaining the homeostatic environment of the cochlea.

9

Docking for drug interface residues of modelled VPS33B of human with PtpA of Mycobacterium tuberculosis CDC1551

84%

Reddy K. M. , Pattathil M. D. , Jayachandra S. Y. , Parameshwar A. , Sulochana M. B.

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tom 11

|

nr 2

EN

VPS33B, a human Vacuolar Protein Sorting (VPS) protein which mediates the phagolysosomal fusion in macrophage of the eukaryotic organisms. This protein has a great role during the mycobacterial infections, which binds with the Mycobacterium protein tyrosine phosphatase A (PtpA). A single functional domain of PtpA has been identified using SMART domain databases, followed by finding the antigenicity of PtpA using CLC main workbench tool. The protein-protein interaction network predicts the interface of biological functions of proteins, built by using Cytoscape 2.8.3 version tool for manual literature survey of protein sets. According to the literature the specific interactivity of PtpA with VPS33B of human lead to pathogenesis, and provided a good platform to find the structure of VPS33B as it lacks the 3 dimensional structure in PDB. Homology Modelling of VPS33B provides a significant properties to design a specific drug through screening the drug databases (eDrug3D). The modelled protein has been validated through SAVES server maintained by NIH and UCLA with the standard Ramachandran plot with accuracy of 90.7 %. From our findings the interface residues are very crucial points which has been found through docking the modelled protein and Mycobacterium protein and interface residues were selected manually using PyMol software.

10

Regulation of lncRNA expression

84%

Wu Z. , Liu X. , Liu L. , Deng H. , Zhang J. , Xu Q. , Cen B. , Ji A.

Cellular and Molecular Biology Letters

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2014

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tom 19

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nr 4

EN

Long non-coding RNAs (lncRNAs) are series of transcripts with important biological functions. Various diseases have been associated with aberrant expression of lncRNAs and the related dysregulation of mRNAs. In this review, we highlight the mechanisms of dynamic lncRNA expression. The chromatin state contributes to the low and specific expression of lncRNAs. The transcription of non-coding RNA genes is regulated by many core transcription factors applied to protein-coding genes. However, specific DNA sequences may allow their unsynchronized transcription with their location-associated mRNAs. Additionally, there are multiple mechanisms involved in the post-transcriptional regulation of lncRNAs. Among these, microRNAs might have indispensible regulatory effects on lncRNAs, based on recent discoveries.

11

H3K4 histone methylation in oral squamous cell carcinoma

84%

Mancuso M. , Matassa D. S. , Conte M. , Colella G. , Rana G. , Fucci L. , Piscopo M.

Acta Biochimica Polonica

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2009

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tom 56

|

nr 3

405-410

EN

Methylation of specific lysine residues in histone tails has been proposed to function as a stable epigenetic marker that directs biological functions altering chromatin structure. Recent findings have implicated alteration in heterochromatin formation as a contributing factor in cancer development. In order to verify whether changes in the overall level of H3K4 histone methylation could be involved in oral squamous carcinoma, the levels of H3K4me1, me2 and me3 were measured in oral squamous carcinoma, leukoplakias and normal tissues. The levels of H3K4me2 and me3 were significantly different in oral squamous cell carcinoma in comparison with normal tissue: the level of H3K4me2 was increased while that of H3K4me3 decreased. No significant differences could be found between the two types of tissues in the level of H3K4me1. A similar trend was found in the leukoplakias that appeared more like the pathological than normal tissue. These results support the idea that alteration of chromatin structure could contribute to oncogenic potential

12

Developmental biology of Arabidopsis thaliana At1g67580 mutant

84%

Kuta E. , Malec P. , Pilarska M. , Slomka A. , Rataj K. , Ilnicki T.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2008

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tom 50

|

nr 1

13

Regulation of bacterial protease activity

84%

Wladyka B. , Pustelny K.

Cellular and Molecular Biology Letters

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2008

|

tom 13

|

nr 2

212-229

EN

Proteases, also referred to as peptidases, are the enzymes that catalyse the hydrolysis of peptide bonds in polipeptides. A variety of biological functions and processes depend on their activity. Regardless of the organism’s complexity, peptidases are essential at every stage of life of every individual cell, since all protein molecules produced must be proteolytically processed and eventually recycled. Protease inhibitors play a crucial role in the required strict and multilevel control of the activity of proteases involved in processes conditioning both the physiological and pathophysiological functioning of an organism, as well as in host-pathogen interactions. This review describes the regulation of activity of bacterial proteases produced by dangerous human pathogens, focusing on the Staphylococcus genus.

14

The role of carotenoids in fish

84%

Wozniak M.

|

1996

|

tom 22

65-74

15

Study on the biological function of Nicotiana tabacum osmotic stress activated protein kinase [NTOSAK]

84%

Pekala I. , Kelner A. , Dobrowolska G.

|

nr 2 Spec.Vol.

16

Human diadenosine triphosphate hydrolase: preliminary characterization and comparison with the human tumor suppressor Fhit protein

84%

Rotllan P. , Asensio A. C. , Oaknin S.

Cellular and Molecular Biology Letters

|

1999

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tom 04

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nr 3

17

Activities of de-N-glycosylation are ubiquitously found in tomato plant

84%

Faugeron C. , Mollet J. C. , Karamanos Y. , Morvan H.

Acta Physiologiae Plantarum

|

2006

|

tom 28

|

nr 6

EN

Activities of two de-V-glycosylation enzymes, PNGase (peptide V4(V-acetyl glucosaminyl) asparagine amidase) and ENGase (endo V-acetyl-P-D-glucosaminidase), involved in the re-ease of N-glycans from V-glycoproteins, were montored in several organs of tomato plants (Lycopersicon esculentum, Mill., cv. Dombito) with a fluoeescence-HPLC procedure using a resofurin-labelled N-glycopeptide substrate. PNGase and ENGase activities were detected in every organ assayed but with quantitative differences. The highest activities were found in the youngest parts of the plant, i.e. apical buds, flowers and leaf blades. PNGase activities were consistently higher than ENGase activities (three-fold in average). Both de-V-glycosylation activities were associated with high levels of proteins and protease activities. During fruit growth and ripening, these three parameters decreased notably. The ubiquitous detection of these enzyme activities in the different organs is probably associated with the previously characterized unconjugated N-glycans in tomato. The possible role of PNGase and ENGase degradation products (i.e. unconjugated N-glycans) are discussed in relation with their biological functions in plant development.

18

Physical properties of lipid bilayer membranes: relevance to membrane biological functions

84%

Subczynski W. K. , Wisniewska A.

|

2000

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tom 47

|

nr 3

EN

Over the last 25 years one of us (WKS) has been investigating physical properties of lipid bilayer membranes. In 1991 a group led by WKS was organized into the Laboratory of Structure and Dynamics of Biological Membranes, the effective member of which is AW. Using mainly the electron paramagnetic resonance (EPR) spin-labeling method, we obtained unexpected results, which are significant for the better understanding of the functioning of biological membranes. We have developed a new pulse EPR spin-labeling method for the detection of membrane domains and evaluation of lipid exchange rates. This review will be focused on our main results which can be summarized as follows: (1) Unsaturation of alkyl chains greatly reduces the ordering and rigidifying effects of cholesterol although the unsaturation alone gives only minor fluidizing effects, as observed by order and reorientational motion, and rather significant rigidifying effects, as observed by translational motion of probe molecules; (2) Fluid-phase model membranes and cell plasma membranes are not barriers to oxygen and nitric oxide transport; (3) Polar carotenoids can regulate membrane fluidity in a way similar to cholesterol; (4) Formation of effective hydrophobic barriers to the permeation of small polar molecules across membranes requires alkyl chain unsaturation and/or the presence of cholesterol; (5) Fluid-phase micro-immiscibility takes place in cis-unsaturated phosphatidylcholine-cholesterol membranes and induces the formation of cholesterol-rich domains; (6) In membranes containing high concentrations of transmembrane proteins a new lipid domain is formed, with lipids trapped within aggregates of proteins, in which the lipid dynamics is diminished to the level of gel-phase.

19

Znaczenie skladnikow mineralnych i witamin w zywieniu szynszyli

67%

Szeleszczuk O. , Niedbala P.

Biuletyn Informacyjny dla Hodowców Szynszyli

|

1998

|

nr 1

17-20

20

Separation, polymer formation and antibacterial activity of lysozyme

67%

Kijowski J. , Lesnierowski G.

|

nr 3

EN

Lysozyme (E.C.3.2.17) is widespread in nature and hen egg white is a rich source of the enzyme. Membrane isolation technique of lysozyme has a limited application because of poor yield and insufficient purity of product. The classic method based on the enzyme precipitation and crystallisation is still being used. The method is often replaced by ion exchange method that is also recommended for large-scale industrial purposes as efficient and not very expensive. The application of gel filtration gives highly homogenous lysozyme with the presence of certain amount of multimeric forms of enzyme. The amount of dimeric and other polymeric forms can be increased by thermal denaturation of the monomeric form. Irreversible formation of the lysozyme dimer makes the enzyme to be quite a novel antimicrobial agent against Gram-negative bacteria. A different mechanism of the action has been suggested for the dimeric form. Lysozyme monomeric form is known as a natural preservative with strong antimicrobial activity, especially against the Gram-positive bacteria. The enzyme extends the shelf-life of certain food products e.g. a row-chilled poultry by reducing the total count of bacteria as well as E.coli and enterococci. Lysozyme greatly affects Listeria monocytogenes and Clostridium botulinum but salmonellas demonstrate some resistance to the enzyme action.