Wyniki wyszukiwania - Biblioteka Nauki

1

The Structure of the O-Specific Polysaccharide of the Lipopolysaccharide from Acinetobacter Strain 44 (DNA Group 3)

100%

Vinogradov E. , Brade L. , Brade H. , Holst O.

Polish Journal of Chemistry

|

2005

|

tom Vol. 79 / nr 2

267-273

EN

Extraction of dry bacteria of Acinetobacter strain 44 (DNA group 3) by phenol/water gave a polymer that was identified by means of serological studies as S-form lipopolysaccharide (LPS). Mild acetic acid hydrolysis degraded the O-specific polysaccharide, thus, its structure was investigated by compositional analyses andNMRspectroscopy after de-O-acylation and Smith degradation of the LPS. The structure of the O-specific polysaccharides was 3)-alfa-L-Rhap-(1_2)-alfa-L-Rhap-(1--3)-beta-D-GlcpNAc-(1--3)-alfa-L-Rhap-(1-- 2 _ 1 alfa-L-Rhap-(1--4)-beta-D-GlcpA-(1--2)-alfa-L-Rhap After immunization of BALB/c mice with Acinetobacter strain 44, monoclonal antibody S48-26 (IgG3 isotype) was obtained which reacted in Western blot with this LPS and characterized it as S-form.

2

Adhezja paleczek Acinetobacter sp. do para-ksylenu

100%

Krasnicki K. , Gospodarek E.

Medycyna Doświadczalna i Mikrobiologia

|

2004

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tom 56

|

nr 2

179-185

PL

W adhezji bakteryjnej ważną rolę wśród oddziaływań niespecyficznych odgrywają hydrofobowe właściwości. Jedną z metod oceny tych właściwości jest adhezja do para-ksylenu. Badano właściwości hydrofobowe 200 szczepów różnych gatunków pałeczek Acinetobacter sp. izolowanych z próbek materiału diagnostycznego. Adhezję do para-ksylenu wykryto u 48,0% szczepów Acinetobacter sp. Najczęściej stwierdzano ją u A. baumannii i A. junii.

EN

Hydrophobicity of 200 of Acinetobacter spp. strains was defined by the BATH (Bacterial Adhesion to Hydrocarbon Test) method of Rosenberg et al. 48,0% strains of Acinetobacter spp. adhered to para-xylene. 40,0% of strains showed waek hydrophobicity, 7,5% moderate and one strain strongly adhered to para-xylene. A. baumannii strains adhered in 52,2%, A. junii in 42,1% and 20,0% in A. haemolyticus. Significantly we find weak adhesion to para-xylene than mild and strong. Mostly adhesion was discovered in A. baumannii and A. junii than in A. haemolyticus. Results show that Acinetobacter spp. has adhesive properties to para-xylene.

3

Mechanizmy opornosci paleczek Acinetobacter spp. na antybiotyki nie beta-laktamowe

88%

Zmudzinski M. , Gospodarek E. , Gierlotka K.

Postępy Mikrobiologii

|

2007

|

tom 46

|

nr 4

335-342

4

Wykorzystanie przez gronkowce sideroforow wytwarzanych przez paleczki z rodzaju Acinetobacter

75%

Szarapinska-Kwaszewska J. , Gospodarek E. , Mikucki J.

Medycyna Doświadczalna i Mikrobiologia

|

1998

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tom 50

|

nr 1-2

9-19

PL

Badano zdolność gronkowców - 24 szczepów należących do 18 gatunków (głównie koagulazoujemnych) do przyswajania żelaza za pośrednictwem sideroforów pałeczek Acinetobacter (7 szczepów z 4 gatunków). Większość badanych szczepów wykazała możliwość posługiwania się tymi egzogennymi chelatorami żelaza.

EN

The ability of iron utilizing by means of siderophores produced by donor strains - the members of the genus Acinetobacter (8 strains) by 24 staphylococcal strains was investigated. All the donor strains synthesized hydroxamate class siderophores and six strains also catecholate class. The majority of staphylococcal strains could utilize these siderophores. Most strains utilized siderophores from A. juni 321 and A. johnsonii 349 strains. Only three staphylococcal strains were not be able to utilize siderophores from all donor strains. The ability of iron utilizing by means of siderophores produced by donor strains - the members of the genus Acinetobacter (8 strains) by 24 staphylococcal strains was investigated. All the donor strains synthesized hydroxamate class siderophores and six strains also catecholate class. The majority of staphylococcal strains could utilize these siderophores. Most strains utilized siderophores from A. juni 321 and A. johnsonii 349 strains. Only three staphylococcal strains were not be able to utilize siderophores from all donor strains.

5

Ocena aktywnosci doripenemu wobec paleczek z rodzajow Pseudomonas sp. i Acinetobacter sp.

75%

Bogiel T. , Deptula A. , Gospodarek E.

Medycyna Doświadczalna i Mikrobiologia

|

2009

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tom 61

|

nr 4

367-374

6

Tigecycline susceptibility in multidrug resistant Acinetobacter isolates from Turkey

75%

Yilmaz F. F. , Tasli H. , Gul-Yurtsever S. , Buyuk A. , Hosgor-Limoncu M.

Polish Journal of Microbiology

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2013

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tom 62

|

nr 3

EN

The present study aimed to evaluate antimicrobial activity of tigecycline against 84 multidrug resistant (MDR) Acinetobacter spp. strains by disc diffusion and E-test methods. The results of disc diffusion test were compared according to two different interpretation ways. In addition, E-test results and the disc diffusion results that interpreted by both the methods were checked for compatibility. According to the disc diffusion test, 3 strains (3.57%) were found resistant to tigecycline when considering breakpoints suggested by Food and Drug Administration (FDA). On the other hand, none of the strains was found resistant to the evaluation criteria recommended by Jones et al. (2007). Considering E-test results of tigecycline, MIC₅₀ and MIC₉₀ values of tigecycline for Acinetobacter spp. were 0.75 and 1 mg/l, respectively. Based on FDA defined breakpoints for Enterobacteriaceae, any resistant isolate was detected. In conclusion, although there are some differences in the results, tigecycline was found quite effective on Acinetobacter spp. isolates with reference to the both disc diffusion and the E-test methods.

7

Comparison of in vitro activity of doripenem, imipenem and meropenem against clinical isolates of Enterobacteriaceae, Pseudomonas and Acinetobacter in Southern Poland

75%

Drzewiecki A. , Bulanda M. , Talaga K. , Sodo A. , Adamski P.

Polish Journal of Surgery

|

2012

|

tom 84

|

nr 9

449-453

EN

In the study we tested drug sensitivity to 3 carbapenems (doripenem, imipenem and meropenem) of Gram-negative clinical isolates from Southern Poland. Material and methods. 89 strains were examined: 42 from Pseudomonas genus, 16 Acinetobacter baumannii strains and 31 Enterobacteriaceae strains. Etests were used according to the producers instructions, MIC values were interpreted using EUCAST criteria. Results. Highest in vitro activity against Pseudomonas spp. was shown for doripenem, then meropenem and the lowest for imipenem (MIC values were definitely lower for doripenem; differences were statistically significant); A. baumannii strains showed similar sensitivity to doripenem, meropenem and imipenem (differences non-significant); all Enterobacteriaceae strains showed sensitivity to the tested antimicrobials. Conclusions. As a conclusion-doripenem, which has high in vitro activity (almost the same as imipenem and meropenem) as well as beneficial pharmacologic properties, may be an alternative solution in the treatment of multiresistant Gram-negative bacteria, especially in patients in severe status who require restrictive antibiotic regimens.

8

Novel therapies of multidrug-resistant Pseudomonas aeruginosa and Acinetobacter spp. infections: the state of the art

75%

Wroblewska M.

Archivum Immunologiae et Therapiae Experimentalis

|

2006

|

tom 54

|

nr 2

9

Siderofory i hemolizyny w pozyskiwaniu zelaza przez paleczki z rodzaju Acinetobacter

63%

Lisiecki P. , Fleischner M. , Wysocki P. , Przondo-Mordarska A. , Mikucki J.

Medycyna Doświadczalna i Mikrobiologia

|

2003

|

tom 55

|

nr 4

379-390

PL

Badaniami objęto 60 szczepów z rodzaju Acinetobacter należących do gatunków A. baumannii, A. haemolyticus, A. junii i A. Iwoffii i izolowanych z różnych materiałów klinicznych. Analizując wyniki statystycznie oceniano zależności między ogólnym poziomem aktywności sideroforowej, wytwarzaniem sideroforów klasy hydroksamowej i fenolano- wo-katecholowej, bezpośrednią i pośrednią hemolizę różnych krwinek a przynależnością gatunkową szczepu jego pochodzeniem i intensywnością wzrostu w pożywce z niedoborem żelaza.

EN

In 60 strains of Acinetobacter genus isolated from clinical material belonging to species A. baumannii, A. haemolyticus, A. junii and A. Iwoffii a hydroxamate and phenol-catechol class siderophores was identified by chemical and biological testes. A correlation between siderophores production and growth intensity, species affiliation and origin of strains was found.

10

Porownawcza ocena identyfikacji bakterii z rodzaju Acinetobacter przy uzyciu komercyjnego systemu API 20NE oraz technika PCR i RFLP

63%

Wydmuch Z. , Kepa M. , Wojtyczka R. , Idzik D. , Pacha J.

Medycyna Doświadczalna i Mikrobiologia

|

2000

|

tom 52

|

nr 3

267-273

PL

Zidentyfikowano bakterie z rodzaju Acinetobacter systemem API 20NE oraz techniką PCR/RFLP. Wykryty gen recA poddano trawieniu endonukleazami restrykcyjnymi Mbol i Hinfl. Restrykcyjna analiza umożliwiła określenie gatunków genowych wszystkich badanych szczepów.

EN

A study was carried out for identification of 50 Acinetobacter strains isolated from various clinical materials. Using classic methods the following species were identifies: Acinetobacter sp. (68%), Acinetobacter baumanii (24%) and Acinetobacter lwofä (8%). In all strains the recA gene was found of 435-500 pz size which confirms their belonging to that genus. Amplification products were digested with restriction enzymes Mbol and Hinfl (RFLP) and their detection was carried out on agarose gel by electrophoresis methods, owing to that the arrangement of gene fragment characteristic of each strain was obtained. After careful analysis restriction patterns were obtained corresponding to the following genome species: Acinetobacter baumanii (60%), Acinetobacter sp. 3 (28%) and Acinetobacter Iwoffii (12%). The methods of molecular biology made possible a more precise classification of the studied strains according to species. Certain strains determined as Acinetobacter sp. by the API 20NE system were found to be Acinetobacter baumanii, Acinetobacter sp. 3 or Acinetobacter Iwofii when determined by the PCR/RFLP method.

11

Filogeneza bakterii z rodzaju Acinetobacter

63%

Kur J. , Lewandowski K. , Krawczyk B. , Samet A.

Postępy Mikrobiologii

|

2000

|

tom 39

|

nr 3

291-301

12

Mechanizmy opornosci paleczek Acinetobacter spp. na antybiotyki beta-laktamowe

63%

Zmudzinski M. , Gospodarek E.

Postępy Mikrobiologii

|

2007

|

tom 46

|

nr 3

236-273

13

Association between existence of integrons and multi-drug resistance in Acinetobacter isolated from patients in Southern Iran

63%

Japoni S. , Japoni A. , Farshad S. , Ali A. A. , Jamalidoust M.

Polish Journal of Microbiology

|

2011

|

tom 60

|

nr 2

EN

Nosocomial infections caused by multi-drug resistant Acinetobacter pose a serious problem in many countries. This study aimed at determining the antibiotic susceptibility patterns and prevalence of different classes of integrons in isolated Acinetobacter. In addition, the association between production of specific bands in PCR assay and magnitude of multi-drug resistance was investigated. In total, 88 Acinetobacter strains were isolated from patients from October 2008 through September 2009. The Minimal inhibitory concentration (MIC) of 12 antibiotics conventionally used in clinics against the isolates, was determined by E-test method. The existence of integron classes was investigated by PCR assay through the amplification of integrase genes. The most effective antibiotic against Acinetobacter was colistin with 97.7% activity, followed by imipenem (77.3%) and meropenem (72.7%). The presence of integron classes 1 and 2 in 47 (53.4%) isolates was confirme, However, no class 3 was detected. The proportion of class 1, compared with class 2, was high (47.7% vs. 3.4%). The association between multi-drug resistance to norfloxacin, ceftazidime, gentamicin, ciprofloxacin, cefepime and amikacin and the presence of integrons was statistically significant. However, the association was not remarkable in many of the isolates which exhibited resistance to the rest of antibiotics. This may imply that in addition to integrons, other resistance determinants such as transposon and plasmid may also contribute to resistance. To reduce the pressure on sensitive isolates, comprehensive control measures should be implemented. Furthermore, wise application of effective antibiotics could help alleviate the situation. Colistin is the most effective antibiotic in vitro against Acinetobacter.

14

Identyfikacja Gram-ujemnych pałeczek niefermentujących

63%

Fleischer M. , Przondo-Mordalska A. , Stankiewicz M.

Diagnostyka Laboratoryjna

|

1989

|

tom 25

|

nr 4-5

15

Rodzaj Acinetobacter - taksonomia i charakterystyka

63%

Nowak A. , Kur J.

Postępy Mikrobiologii

|

1995

|

tom 34

|

nr 3

271-287

16

Znaczenie kliniczne bakterii z rodzaju Acinetobacter - nowe zagrozenia

63%

Wroblewska M.

Postępy Mikrobiologii

|

2008

|

tom 47

|

nr 3

345-352

17

The KPC type beta-lactamases: new enzymes that confer resistance to carbapenems in gram-negative bacilli

51%

Sacha P. , Ostas A. , Jaworowska J. , Wieczorek P. , Ojdana D. , Ratajczak J. , Tryniszewska E.

|

2009

|

tom 47

|

nr 4

18

Wplyw chlodzenia na mikroorganizmy. Chlodzenie i przechowywanie chlodnicze miesa. Czesc II

51%

Mięso i Wędliny

|

1995

|

nr 2

8

19

Sklad mikroflory na wychlodzonej wolowinie

51%

Mięso i Wędliny

|

2008

|

nr 3

50

20

Czestosc wystepowania i lekooopornosc niefermentujacych paleczek Gram-ujemnych izolowanych od pacjentow hospitalizowanych na oddzialach intensywnej terapii szpitala klinicznego

51%

Wroblewska M. , Marchel H. , Rudnicka J. , Luczak M.

Medycyna Doświadczalna i Mikrobiologia

|

2005

|

tom 57

|

nr 2

217-224

PL

Niefermentujące pałeczki Gram-ujemne są patogenami oportunistycznymi odgrywającymi coraz większą rolę w zakażeniach pacjentów leczonych na oddziałach intensywnej terapii (OIT). Celem pracy była ocena występowania i Iekowrażliwości niefermentujących pałeczek Gram-ujemnych izolowanych od tej grupy chorych, z uwzględnieniem profilu OIT. Stwierdzono różnice w częstości izolacji tych bakterii od pacjentów z różnych oddziałów oraz występowanie oporności szczepów Acinetobacter sp. na karbapenemy.

EN

The aim of the study was to assess frequency and susceptibility to antimicrobial agents of non- fermenting Gram-negative rods isolated from clinical specimens obtained from patients requiring intensive care, with emphasis on profile of the unit. Identification of cultured isolates was done using automated VITEK and API systems (bioMerieux, France). Susceptibility to antimicrobial agents was tested by a disk-diffusion method according to the NCCLS recommendations. In total the analysis comprised 425 strains of non-fermenting Gram-negative rods, constituting 58,9% of all isolates of Gram-negative bacteria. In blood cultures predominated strains of A. baumannii (46,8%) and P aeruginosa (40,4%), while in cultures of other clinical specimens these bacteria comprised 42,9% and 43,9% of isolates. Major differences were observed in frequency of these species on both ICU units. Strains of non-fermenting rods isolated from blood cultures comprised a lower percentage of strains susceptible to antimicrobials (particularly cefepime and carbapenems) than isolates cultured from other specimens. Strains of A. baumannii resistant to imipenem and meropenem were detected with a frequency of 12,5% and 26,7%, respectively. Resistance of P. aeruginosa strains to carbapenems was 62,2% and 44,3%, respectively. There was a relatively high percentage of strains susceptible to cefepime (82,0%), ceftazidime (78,9%), amikacin (77,8%) and piperacillin/tazobactam (69,7%). Conclusions: 1. There was a predominance (58,9%) of strains of Gram-negative non-fermenting rods. 2. Isolates from blood cultures were characterised by a much higher percentage of resistant strains in comparison to other specimens. 3. Strains of A. baumannii resistant to carbapenems were recorded. 4. There were differences in frequency and antimicrobial susceptibility among the strains of P. aeruginosa і A. baumannii depending on the type of clinical specimen and ICU profile.