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1

Wplyw fruktanow o roznym stopniu polimeryzacji lancucha weglowodanowego na procesy fermentacyjne w koncowym odcinku przewodu pokarmowego u szczurow doswiadczalnych

100%

Zary-Sikorska E. , Juskiewicz J.

Żywność Nauka Technologia Jakość

|

2007

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tom 14

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nr 5

385-391

PL

W przeprowadzonym doświadczeniu zbadano wpływ dwóch typów fruktanów o różnym stopniu polimeryzacji łańcucha węglowodanowego: krótkołańcuchowych fruktooligosacharydów (FOS) oraz długołańcuchowej inuliny na fizjologię jelita ślepego u szczurów. W badaniach zastosowano trzy diety: dietę kontrolną zawierającą 7,5% sacharozy i dwie diety doświadczalne zawierające: 8,3% preparatu inuliny (IN) i 7,9% preparatu FOS (FOS) (preparaty komercyjne). Młode samce rasy Wistar (8 osobników w grupie) otrzymywały diety ad libitum przez 4 tygodnie. Określono indywidualne spożycie paszy oraz przyrost masy ciała. Pomiarowi podlegały m.in.: masa tkanki i treści jelita ślepego, pH, sucha masa, amoniak, białko, lotne kwasy tłuszczowe (LKT) i aktywność enzymów bakteryjnych w treści jelita ślepego. Nie stwierdzono statystycznie istotnej różnicy pomiędzy grupami w wielkości spożycia diet oraz przyrostu masy ciała. Spożycie diet zawierających FOS i inulinę spowodowało znaczny wzrost masy ściany i treści jelita ślepego (P < 0,05) w stosunku do grupy kontrolnej. W grupach doświadczalnych stwierdzono także wzrost koncentracji białka oraz suchej masy w treści jelitowej. Istotnie niższe (P < 0,05) wartości pH i koncentrację amoniaku odnotowano u zwierząt żywionych dietami z FOS i inuliną. Suplementacja diet fruktanami spowodowała wzrost zawartości LKT w przeliczeni na 100 g masy ciała (pula LKT). Inulina, korzystniej niż FOS wpływała na produkcję LKT, zwłaszcza istotnie zwiększając zawartość kwasu propionowego i masłowego w treści jelita ślepego. Inulina w sposób bardziej korzystny niż FOS oddziaływała również na aktywność enzymatyczną mikroflory jelita ślepego. W grupie IN obserwowano najniższą aktywność β-glukuronidazy, która jest biomarkerem aktywności mikroflory patogennej.

EN

In the experiment accomplished, the effect was investigated of two types of fructans: short-chain fructooligosaccharides (FOS) and long-chain inulin, on the blind gut physiology in rats. Three diets were applied in the experiment: control diet (K) contained 7.5% of saccharose, and two other experimental diets contained: 8.3% of inulin (IN) and 7.9% of the FOS preparation (FOS) (commercial preparations). Young male rats of the Wistar breed (8 rats per one group) were fed ad libitum during 4 weeks. The individual feed consumption rates and gains in body weight of rats were determined. The following parameters was measured, among other things: the weight of tissue and of blind gut fill, pH, dry matter, ammonia, protein, volatile fatty acids (VFA), and activity of bacterial enzymes in the blind gut fill. No statistically significant differences in the total dietary food intake rate and in gains in body weight of rats among the experimental groups were found. The consumption of diets containing FOS and inulin caused a considerable increase in the weight of blind gut wall and fill (P < 0.05) compared to the control group. In the experimental groups, an increase was found in the concentration rates of protein and dry matter in the blind gut fill. The significantly lower (P < 0.05) values of pH and ammonia contents were found in the animals fed with the dietary food containing FOS and inulin. When fructans were added to the dietary food, the level of VFA increased per 100g of the body weight (LFA pool). The inulin impacted the production of VFA more beneficially than FOS, especially, it generated a statistically significantly increase in the content levels of propionate and butyrate acids in the blind gut fill. Furthermore, the inulin impacted the enzymatic activity of blind gut micro-flora more effectively than FOS. In the IN group, the lowest level of the activity of β-glucuronidase was found, whereas the β-glucuronidase is a biomarker of the activity of pathogenic micro-flora.

2

Wplyw fruktooligosacharydow i polifenoli z cykorii na procesy fermentacyjne zachodzace w koncowym odcinku przewodu pokarmowego u szczurow doswiadczalnych

100%

Zary-Sikorska E. , Juskiewicz J.

Żywność Nauka Technologia Jakość

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2010

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tom 17

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nr 5

191-199

PL

W wykonanym doświadczeniu badano wpływ fruktanów i polifenoli, równocześnie występujących w diecie, na funkcjonowanie końcowego odcinka przewodu pokarmowego u szczurów doświadczalnych. Zastosowano trzy diety, w tym kontrolną zawierającą 7,5 % sacharozy oraz dwie doświadczalne zawierające: 1) 7,9 % komercyjnego preparatu fruktooligosacharydów (FOS) 2) 8,3 % koncentratu FOS uzyskiwanego z korzeni cykorii, który równocześnie wprowadzał do diety polifenole na poziomie 0,0 5% (FOS+PP). Młode samce rasy Wistar (8 osobników w grupie) otrzymywały paszę ad libitum przez 4 tygodnie. Określono indywidualne spożycie paszy oraz przyrost masy ciała. W treści jelita ślepego oznaczano pH, zawartość: suchej masy, amoniaku, białka, lotnych kwasów tłuszczowych (LKT) oraz aktywność enzymów bakteryjnych. Ważono również jelito ślepe z treścią i bez treści. Nie stwierdzono statystycznie istotnej różnicy pomiędzy grupami pod względem wielkości spożycia paszy oraz przyrostu masy ciała. Spożycie pasz zawierających preparat FOS komercyjny i preparat FOS+PP z korzeni cykorii spowodowało znaczny wzrost masy ściany i treści jelita ślepego (P<0,05) w stosunku do grupy kontrolnej. Istotnie niższe (P < 0,05) wartości pH i mniejsze stężenie amoniaku w treści jelita ślepego odnotowano u zwierząt żywionych paszami doświadczalnymi. Badane preparaty istotnie podwyższyły pulę LKT, zwłaszcza istotnie zwiększając zawartość kwasu propionowego i masłowego w przeliczeni na 100 g masy ciała. W grupie FOS+PP odnotowano istotny wzrost koncentracji LKT w treści jelita ślepego (P < 0,05 vs grupy FOS i K). Diety doświadczalne korzystnie modyfikowały aktywność bakteryjnej -glukozydazy oraz -glukuronidazy.

EN

In the experiment accomplished, the effect was studied of fructans and polyphenols, concurrently occurring in a diet, on the functioning of the final section of alimentary tract in experimental rats. Three diets were applied: one control diet (K) with 7.5 % of sucrose and two experimental diets containing: 1) 7.9 % of commercial fructooligosacharride preparation (FOS) and 2) 8.3 % of FOS concentrate made from chicory roots that also enriched the diet with 0.05 % of polyphenols (FOS+PP). Young male Wistar rats (8 rats per group) were ad libitum fed for 4 weeks. The individual feed consumption level and body weight gains of rats were determined. In the caecal contents, the following parameters were measured: pH, dry matter, ammonia, Lowry’s protein, volatile fatty acids (VFA), and activity of bacterial enzymes. Also, the caecum with and without contents was weighed. No statistically significant differences were found between the groups as regards the feed consumption level and body weight gains in rats. The consumption level of feed products containing commercial FOS and the FOS+PP preparation made from chicory roots caused the weight of caecum wall and of its contents to significantly increase (P<0.05) compared to the control group. Significantly lower (P < 0.05) pH values and a lower ammonia concentration in the caecal contents were found in the animals fed with experimental diets. The preparations analysed significantly increased the SCFA quota, especially, they significantly increased the content of propanoic acid and butyric acid when converted to 100 g of body weight. In the FOS+PP group, a significant increase in the concentration of VFA was found in the caecal contents (P<0.05 vs FOS and K). The experimental diets beneficially modified the activity of bacterial -glucosidase and -glucuronidase.

3

An analysis of consumers’ seafood purchasing preferences in Poland

100%

Zary-Sikorska E. , Wenda-Piesik A.

Journal of Agribusiness and Rural Development

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2016

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nr 3[41]

EN

Seafood consumption in Poland is regularly increasing, although it still remains at a relatively low level compared to the consumption of fi sh and their products. The aim of the study was to obtain information on the frequency of seafood consumption and purchasing preferences among consumer groups in both a large and a small urban center, taking into account not only the place of residence, but also respondents’ age and gender. The study was attended by 204 people (50 men and 154 women). A survey questionnaire verifi ed in a pilot survey was used as a research tool. The research was carried out in a small (approx. 4,000 inhabitants) and a large (approx. 400,000 inhabitants) urban center on consumers going in and out of shops. A factor that signifi cantly infl uenced all the studied characteristics and purchasing preferences was the respondents’ age. The respondents’ gender signifi cantly infl uenced the recognition of diff erent seafood types, the place of consumption and the motivation for purchase. The size of the place of residence was the least infl uential factor in the study. There is a strong need to inform Polish consumers about seafood and the possibility of its consumption.

PL

Spożycie owoców morza w Polsce regularnie wzrasta, aczkolwiek nadal jest na niskim poziomie w odniesieniu do spożycia ryb i ich przetworów. Celem niniejszej pracy było uzyskanie informacji na temat częstotliwości spożycia oraz preferencji nabywczych dotyczących owoców morza wśród grup konsumenckich dużego i małego ośrodka miejskiego, biorąc pod uwagę – oprócz wielkości miejsca zamieszkania – również wiek oraz płeć ankietowanych. W badaniu wzięły udział 204 osoby, w tym 50 mężczyzn oraz 154 kobiety. Narzędziem badawczym był autorski kwestionariusz wywiadu zweryfikowany w badaniu próbnym. Badanie przeprowadzono w małym (ok. 4 tys. mieszkańców) i dużym (ok. 400 tys. mieszkańców) ośrodku miejskim wśród konsumentów dokonujących zakupów. Wiek respondentów był czynnikiem istotnie wpływającym na wszystkie badane cechy i preferencje nabywcze. Płeć respondentów istotnie wpływała na rozpoznawalność rodzajów owoców morza, miejsce ich spożycia oraz motywacje do zakupu. Wielkość miejsca zamieszkania była najmniej istotną cechą w badaniach. Istnieje silna potrzeba popularyzacji wiedzy dotyczącej owoców morza i możliwości ich spożycia wśród polskich konsumentów.

4

Effect of fructans with different degrees of polymerization on bacterial enzymes activity, lipid profile and antioxidant status in rats

100%

Zary-Sikorska E. , Juskiewicz J.

Polish Journal of Food and Nutrition Sciences

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2008

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tom 58

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nr 2

EN

The aim of the study was to compare the effect of two types of fructans characterised by a different degree of polymerization (long-chain inulin and short-chain fructooligosaccharides [FOS]), added as single dietary components on the activity of selected bacterial enzymes in the faeces and the caecal digesta, the antioxidant status and the serum lipid profile in laboratory rats. Twenty four male Wistar rats were randomly divided into three groups and for 4 weeks were fed a control casein and two experimental diets supplemented with fructooligosaccharides and inulin at a level of 7.5% of diet (C, FOS and IN groups, respectively). The inulin addition caused a significant decrease in faecal pH value throughout the study (pH measurements were taken after day 7, 14, 21 and 28 of feeding), whereas the FOS-diet significantly decreased pH of fresh faeces only after 3 weeks of the study (p<0.05 vs. the control). Both experimental diets beneficially diminished the activity of faecal β-glucosidase and β-glucuronidase as compared to the control animals. The decreased activity of bacterial β-glucosidase and increased activity of β-galactosidase in the caecal digesta was noted only in the case of dietary FOS. The lowest concentrations of TBARS in the liver, kidneys and serum were observed in the IN group (p<0.05 vs. the control), however the FOS diet also effectively reduced concentration of TBARS in the liver tissue and the serum. The dietary inulin significantly decreased the serum glucose level and increased the percentage of HDL-cholesterol in total cholesterol (vs. C and FOS groups). The results obtained demonstrate that the consumption of diet supplemented with inulin seems to be more health promoting and elicits more beneficial changes in microflora activity, lipid metabolism and antioxidant status in rats than that enriched with short-chain fructooligosaccharides.

5

Preferencje konsumentów do spożycia kolorowych odmian marchwi

80%

Zary-Sikorska E. , Wichrowska D. , Gozdecka G. , Gesinski K.

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2015

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tom 23

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nr 6

6

Analiza sensoryczna przetworów z mało znanych odmian marchwi

80%

Zary-Sikorska E. , Wichrowska D. , Gozdecka G. , Gesinski K.

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2015

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tom 23

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nr 5

7

Wartość wypiekowa mąki pszennej i jej mieszanek z mąką orkiszową

61%

Knapowski T. , Kozera W. , Wszelaczynska E. , Poberezny J. , Mozolewski W. , Wichrowska D. , Zary-Sikorska E.

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2015

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tom 23

|

nr 6

8

Role of glutathione metabolism and glutathione-related antioxidant defense systems in hypertension

61%

Robaczewska J. , Kedziora-Kornatowska K. , Kozakiewicz M. , Zary-Sikorska E. , Pawluk H. , Pawliszak W. , Kedziora J.

Journal of Physiology and Pharmacology

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2016

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tom 67

|

nr 3

9

Concentrations of blood serum and urinal ellagitannin metabolites depend largely on the post-intake time and duration of strawberry phenolics ingestion in rats

61%

Zary-Sikorska E. , Kosmala M. , Milala J. , Fotschki B. , Ognik K. , Juskiewicz J.

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tom 69

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nr 4

EN

The different duration of a strawberry phenolic fraction intake and different post-intake time were experimental factors affecting the concentrations of ellagitannin metabolites in the urine and blood serum of rats. For four days, the animals were gavaged once a day as follows: group C (water, days 1–4), group F1–4 (fraction, days 1–4), group F1–3 (fraction, days 1–3; water, day 4), group F1–2 (fraction, days 1, 2; water, days 3, 4), group F3–4 (water, days 1, 2; fraction, days 3, 4), and group F4 (water, days 1–3; and fraction, day 4). The daily dosage of the fraction gavaged to one rat was 20 mg/kg of body weight. The fraction contained monomers and dimers of ellagitannins as well as proanthocyanidins. The caecal, urinal, and blood serum ellagitannin metabolites were analysed 12 h after the last treatment. Ellagic acid, urolithin A, and nasutin A were detected in the caecal digesta. In turn, urolithin A, nasutin A, and their glucuronide conjugates were detected in the urine, while urolithin A glucuronide, nasutin A glucuronide, and ellagic acid dimethyl ether glucuronide were found in the serum. The highest caecal and urinal concentrations of ellagitannin metabolites followed the F1–4 treatment. In the serum, the highest concentrations of the metabolites were determined in the rats administered the phenolic fraction during days 1–4 and 3–4. No metabolites were found in the rats following the C and F1–2 treatments. The results suggest that the presence of ellagitannin metabolites in the rat’surine and serum largely refl ects the concentrations of caecal metabolites and the number of subsequent days of strawberry phenolic fraction administration.

10

Physiological properties of dietary ellagitannin-rich preparations obtained from strawberry pomace using different extraction methods

61%

Juskiewicz J. , Krol B. , Kosmala M. , Milala J. , Zdunczyk Z. , Zary-Sikorska E.

Polish Journal of Food and Nutrition Sciences

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2015

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tom 65

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nr 3

EN

The objective of this study was to establish the composition of strawberry preparations rich in ellagitannins obtained using water or acetone extraction (EF and EP preparation, respectively). Then, biological effect of these extracts was assessed in 4-wk nutritional experiment on Wistar rats. The preparations were applied in cholesterol-containing diets that had equal content of ellagitannins (0.03%). To measure animals response, parameters describing the caecal fermentation (ammonia and short-chain fatty acid concentrations, bacterial enzymes activity), blood serum lipoprotein profile, and TBARS content in selected tissues (heart, liver, kidney) were assessed. Apart from polyphenols, including ellagitannins (7.8 and 7.1%, respectively), the EF preparation contained high quantities of soluble dietary fibre and other carbohydrates (33.3 and 38.9%, respectively), whereas the EP preparation was characterised by 58.9% content of ellagitannins, no dietary fibre and a high content of proanthocyanidins (16.9%). In comparison to EF group, the dietary treatment with EP had a stronger effect on caecal environment as manifested by decreased digesta bulk, ß-glucuronidase activity and total short-chain fatty acid concentration (P<0.05 vs. group C without supplementation). Both preparations lowered lipaemia and glycaemia. It could be concluded that more efficient acetone extraction of strawberry pomace increased the content of both ellagitannins and proanthocyanidins in the polyphenolic preparation, which caused a stronger inhibiting effect on caecal fermentation processes and at the same time lowered blood triacyl-glycerols and glucose level. Considering the equal content of ellagitannins in both supplemented diets, it may be speculated that the above effects were due to the presence of proanthocyanidin fraction.

11

Ocena efektywności produkcji ziemniaka jadalnego po wprowadzeniu ograniczeń w technologii uprawy

51%

Wszelaczynska E. , Poberezny J. , Szrejber T. , Keutgen A. J. , Zary-Sikorska E. , Wichrowska D. , Knapowski T. , Kozera W.

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2015

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tom 23

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nr 5

12

Synergistic antimicrobial effect of raspberry (Rubus idaeus L., Rosaceae) preparations and probiotic bacteria on enteric pathogens

51%

Bauza-Kaszewska J. , Zary-Sikorska E. , Gugolek A. , Ligocka A. , Kosmala M. , Karlinska E. , Fotschki B. , Juskiewicz J.

Polish Journal of Food and Nutrition Sciences

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2021

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tom 71

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nr 1

EN

Due to the increasing microbial tolerance to commonly used food preservatives, as well as growing consumer awareness of their adverse impact on human health, alternative methods of pathogens reduction in food are widely investigated. The aim of this research was to examine the antimicrobial activity of red raspberry pomace and seed preparations against enterohemorrhagic Escherichia coli (EHEC), Salmonella Typhimurium, Salmonella Enteritidis, Listeria monocytogenes, and probiotic Lactobacillus rhamnosus strain. The combined action of LAB (lactic acid bacteria) and raspberry preparations on the pathogenic species was also evaluated. The results of our study showed no or weak antibacterial effect of raspberry preparations on the pathogenic bacteria tested. Regardless of preparation concentration (1.0 or 2.0 mg/mL), the bacteria number after 48-h incubation was usually higher than in the culture at the initial stage and varied from 10⁵ to 10⁷ cfu/mL. On the other hand, probiotic Lactobacillus rhamnosus strain caused a significant reduction in the enteric pathogen count after 24-h co-culture with LAB. The concentrations of both Salmonella serotypes were below the detection limit of the analytical methods applied. Moreover, the combined use of LAB and raspberry preparations resulted in the total elimination of Salmonella strains and the reduction in L. monocytogenes number from 10⁵ to 10²–10⁴ cfu/mL after 24-h co-culture. EHEC revealed the highest resistance to the mixed culture effect. The synergic antimicrobial effect suggests the possibility of applying probiotic bacteria and berry preparations as natural antimicrobial agents in the food industry.