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EN
PCR-RFLP method was used to identify polymorphisms in the 136 (A/V), 154 (R/H), and 171 (R/H/Q) codons of the prion protein gene (PRNP) in 174 ewes and rams of the prolific Olkuska sheep breed from three nucleus flocks. Alanine (A) at codon 136, arginine (R) at codon 154, and arginine (R) or glutaminę (Q) at codon 171, responsible for the presence of two alleles (ARR and ARQ) and three genotypes, (ARR/ARR, ARR/ARQ and ARQ/ARQ) were found in the analysed population. In all flocks, ARR allele (the most resistant to scrapie) was the dominant haplotype regardless of sheep sex, and ARR/ARQ genotype had the highest frequency (60.92%). The proportion of the undesirable ARQ/ARQ genotype was only 4.02%. Simulation of genotype distribution for the next generations showed that the mating of ewes with ARR/ARR genotype rams will cause this genotype to appear in 99% of Olkuska ewes already in the sixth generation. However, the study showed no relationship between genotype in the PRPN locus and prolificacy potential of the ewes.
EN
The study was aimed at determining the age-depended changes in insulin-like growth factors 1 and 2 (IGF-1 and IGF-2) mRNA levels in relation to the gastrointestinal tract (GIT) development in newborn calves. Twenty four male calves (5-day-old) were randomly allocated to 4 groups which were slaughtered at day 5, 12, 19 and 26 of their life, respectively. Tissue samples from the rumen, abomasum, duodenum, jejunum and liver were taken for analysis, and the level of IGF-1 and IGF-2 mRNA was determined using RT semi-quantitative PCR method. Both IGF-1 and IGF-2 mRNA level was the biggest on 5 day of life, significantly decreased up to day 12-19 in most of analyzed GIT parts, and started to increase thereafter. In the rumen the reduction in the amount of IGFs transcripts was associated with the reduction in papillae length and tunica muscularis thickness. The abomasum weight and tunica mucosa thickness increased from the 5 day up to 19 day of life, whereas abomasal IGF-1 mRNA level decreased together with calf's age up to 19 day of life, and the level of IGF-2 mRNA did not change. The reduction in IGFs mRNA level after 5 day of life in the duodenum (IGF-1 and IGF-2) and in the jejunum (IGF-1) was associated with reduction in villi length (duodenum and jejunum), and the increase of crypt depth (duodenum).
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