Wyniki wyszukiwania - Biblioteka Nauki

1

Kryteria mikrobiologiczne srodkow spozywczych

100%

Szteyn J. , Wiszniewska-Laszczych A. , Ruszczynska A.

Higiena

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2006

|

nr 1

15-16

2

Czym truja nas producenci mleka. Soda i woda

100%

Szteyn J. , Wiszniewska-Laszczych A. , Ruszczynska A.

Bezpieczeństwo i Higiena Żywności

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2006

|

nr 10

29-30

3

Zafalszowanie mleka woda

100%

Wiszniewska-Laszczych A. , Szteyn J. , Ruszczynska A.

Higiena

|

2006

|

nr 2-3

15

4

Effectiveness of Mycobacterium paratuberculosis isolation from raw milk by means of direct isolation of DNA and classic culture

100%

Szteyn J. , Wiszniewska-Laszczych A. , Ruszczynska A.

Polish Journal of Veterinary Sciences

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2008

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tom 11

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nr 1

25-28

EN

Detection of Mycobacterium paratuberculosis (MAP) in tissues of patients suffering from Crohn's disease has given rise to speculation that this mycobacterium may play some role in the development of this disease in humans. Food products, especially milk obtained from animals infected with paratuberculosis, may be a potential vector of MAP to humans, yet the detection of this pathogen poses a number of difficulties. This study was aimed at comparing the effectiveness of MAP isolation from milk samples. Mycobacteria were detected by means of two methods: direct isolation of DNA using a QIAamp DNA Mini Kit by Qiagen, and a culture method with the use of HEYM culture medium. Analyses were carried out on 87 samples of udder cow milk originating from a herd that exhibited seropositive and serodoubtful reactions against paratuberculosis. The presence of an insertion sequence IS-900 was detected in 18 samples of udder milk analyzed with the method of direct DNA isolation and in two samples analyzed by means of the culture method.

5

Wystepowanie Escherichia coli O157:H7 w mleku surowym

100%

Ruszczynska A. , Szteyn J. , Wiszniewska-Laszczych A.

Higiena

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2006

|

nr 4

35-36

6

Wystepowanie Mycobacterium paratuberculosis w mleku surowym

100%

Szteyn J. , Wiszniewska-Laszczych A. , Ruszczynska A.

Medycyna Weterynaryjna

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2006

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tom 62

|

nr 10

1186-1187

EN

The aim of the study was to determine the frequency of Mycobacterium paratuberculosis occurrence in raw milk samples from north-eastern Poland. Samples of udder milk were collected from herds in which seropositive reactions on paratuberculosis had been confirmed in samples of bulk tank milk from production farms. Standardization and decontamination of milk samples from fast growing microflora was carried out prior to research which was performed by using the culture method. PCR technique was used to distinguish MAP from other mycobacterium, thus enabling the IS-900 fragment to be detected. The presence of MAP was confirmed in 2.4% of udder milk samples from north-eastern Poland, but none was confirmed in bulk tank milk samples from this region.

7

PCR w wykrywaniu Mycobacterium paratuberculosis w mleku surowym

100%

Szteyn J. , Wiszniewska-Laszczych A. , Ruszczynska A.

Medycyna Weterynaryjna

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2007

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tom 63

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nr 09

1106-1107

EN

The aim of the study was to detect Mycobacterium paratuberculosis DNA in raw milk samples. DNA from 103 udder milk samples was isolated using the eQIAamp DNA Mini Kit (Qiagen). IS-900 - a part of genome characteristic for MAP - was detected in 21 samples.

8

Flow cytometry as a method for the evaluation of raw material, product and process in the dairy industry

100%

Ruszczynska A. , Szteyn J. , Wiszniewska-Laszczych A.

Polish Journal of Veterinary Sciences

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2007

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tom 10

|

nr 2

EN

Producing dairy products which are safe for consumers requires the constant monitoring of the microbiological quality of raw material, the production process itself and the end product. Traditional methods, still a "gold standard", require a specialized laboratory working on recognized and validated methods. Obtaining results is time- and labor-consuming and do not allow rapid evaluation. Hence, there is a need for a rapid, precise method enabling the real-time monitoring of microbiological quality, and flow cytometry serves this function well. It is based on labeling cells suspended in a solution with fluorescent dyes and pumping them into a measurement zone where they are exposed to a precisely focused laser beam. This paper is aimed at presenting the possibilities of applying flow cytometry in the dairy industry.

9

Selenium supplementation into diets containing carnosic acid, fish and rapeseed oils affects the chemical profile of whole blood in lambs

88%

Czauderma M. , Bialek M. , Krajewska K. A. , Ruszczynska A. , Bulska E.

Journal of Animal and Feed Sciences

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2017

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tom 26

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nr 3

EN

The concentration of macro and trace elements, fatty acids (FAs), vitamins, total cholesterol (TCh) in blood as well as in other tissues can be modulated by diet composition. Thus, the purpose of the present study was to evaluate the effects of fish oil (FO), carnosic acid (CA) and selenized-yeast (SeY) or selenate (SeVI) on concentration of FAs, TCh, α-tocopherol (αT) and selected elements in whole blood of lambs. Thirty male lambs were allocated into 5 groups of 6 animals each and fed for 35 days the following diets: control – basal diet (BD) with 3% rapeseed oil (RO), ROFO – BD with 2% RO and 1% FO, CA – BD with 2% RO, 1% FO and 0.1% CA, CASeY – BD with 2% RO, 1% FO, 0.1% CA and 0.35 mg Se as selenized-yeast (SeY) per kg of BD and CASeVI – BD with 2% RO, 1% FO, 0.1% CA and 0.35 mg Se as sodium selenate (SeVI) per kg of BD. In animals fed CASeVI diet the levels of saturated (SFAs), mono- and polyunsaturated FAs, thrombogenic-SFAs and atherogenicSFAs decreased in comparison to the control group. On the other hand, in lambs fed CASeY diet the concentration of TCh in blood increased in comparison to lambs fed CA and CASeVI diets. Moreover, feeding CASeY diet also enhanced the concentration of αT in blood as compared to the animals fed ROFO and CASeVI diets. The lowest αT concentration in blood was noted in blood of lambs fed CASeVI diet. Feeding diets supplemented with SeY or SeVI increased the concentrations of Se and malondialdehyde in blood in comparison to other diets. So, the whole blood can be treated as the valuable non-invasive marker for evaluation of ruminant health status and nutritional quality of ruminant feeds.

10

Paratuberkuloza w stadzie bydla mlecznego na Zulawach

88%

Wiszniewska-Laszczych A. , Szteyn J. , Ruszczynska A. , Hebel T. , Lipiec M.

Medycyna Weterynaryjna

|

2009

|

tom 65

|

nr 07

484-486

EN

Paratuberculosis is a chronic, ulcerating enteritis induced by Mycobacterium avium subsp. paratuberculosis (MAP). It affects domesticated and wild ruminants throughout the world and causes significant economic losses. The opinion that the eradication of tuberculosis has resulted in the elimination of MAP-infected animals and that the disease no longer occurs in Poland, together with difficulties in the diagnosis of the disease, often complicated by other afflictions, the long period of its development and its dubious clinical picture, cause that herd infection might remain unrecognized for a number of years. Dyspepsia proceeding with periodical watery diarrhea was observed on a farm with 250 dairy cows located in Żuławy Wiślane, in the north-eastern region of Poland. By the elimination of epizootic diseases, the conclusion was that the most probable cause of the disease is paratuberculosis. It was proved by the serological tests of cows with clinical symptoms and cows which had manifested symptoms earlier. All blood samples from cows with clinical symptoms and two samples from clinically healthy cows had positive results for paratuberculosis. The subsequent serological test showed that 8.6% of the herd population was infected by MAP. An autopsy performed instantly after anaesthetizing the animals with clinical symptoms showed changes typical for paratuberculosis. Regular examination, the isolation and elimination of infected animals, as well protection of new born calves should be the method used to restrain paratuberculosis in the herd. It could be useful to certify the herds and transport the animals only between farms free from paratuberculosis.

11

Liver and body mass gain, content of conjugated linoleic acid [CLA] isomers and other fatty acids in the liver of rats fed CLA isomers and selenium

75%

Czauderna M. , Kowalczyk J. , Niedzwiedzka K. M. , Wasowska I. , Pastuszewska B. , Bulska E. , Ruszczynska A.

Journal of Animal and Feed Sciences

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2004

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tom 13

|

nr 2

353-369

12

Effect of dietary CLA isomers on selenium, zinc, copper, chromium, magnesium and calcium levels in rat liver

75%

Czauderna M. , Kowalczyk J. , Bulska E. , Boldizarova K. , Niedzwiedzka K. M. , Ruszczynska A. , Leng L.

Journal of Animal and Feed Sciences. Supplement

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2005

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tom 14

|

nr 1

13

Bio-transformation of selenium in Se-enriched bacterial strains of Lactobacillus casei

75%

Kurek E. , Ruszczynska A. , Wojciechowski M. , Luciuk A. , Michalska-Kacymirow M. , Motyl I. , Bulska E.

Roczniki Państwowego Zakładu Higieny

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2016

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tom 67

|

nr 3

EN

Background. Selenium is an element of very great importance for the proper functioning of the human body, mainly due to its antioxidant properties. Selenium exhibits a preventive effect in the case of cardiovascular disease, the immune system, male infertility and inhibits the toxic action of other agents. Selenium is important for Hashimoto’s disease. Intake of selenium in the diet slows the aging process. The biological and toxicological effects of selenium strongly depend on its chemical form. Some organisms for example: plant, yeast, are capable of metabolizing low bioavailable selenium compounds (inorganic selenium) into its high bioavailable forms (organic selenium). Objective. The aim of this study was to investigate the bio-transformation of selenium by Lactobacillus bacteria towards the characterisation of selenium metabolites. Material and Methods. The speciation of selenium was evaluated by high performance liquid chromatography with inductively coupled plasma mass spectrometry detector. The extraction of selenium species from lyophilized bacteria was executed with water, the mixture of lipase and protease, as well as lisozyme and sodium dodecyl sulphate. Results. All investigated bacteria strains cultivated in the presence of Na2SeO3 effectively uptake selenium. Surprisingly, none of the applied extraction media exhibited a strong power to release the majority of the uptaken selenium compounds. Thus a maximum of 10% of the selenium was extracted from bacteria exposed to the enzymes. However, it was found that Lactobacillus bacteria are able to metabolize inorganic ions of selenium (IV) into Se-methionine, Se-methyloselenocysteine and other unidentified forms. Conclusions. The study confirmed the ability of probiotic bacteria to biotransform inorganic selenium into its organic derivatives. Therefore, Se-enriched bacteria can be considered as an addition to the functional food.

PL

Wprowadzenie. Selen jest pierwiastkiem o bardzo dużym znaczeniu dla prawidłowego funkcjonowania organizmu człowieka, głównie ze względu na swoje właściwości antyoksydacyjne. Selen odgrywa ważną rolę w profilaktyce chorób układu sercowo-naczyniowego, układu immunologicznego, niepłodności, a także chroni przed szkodliwym działaniem substancji toksycznych. Selen jest istotny dla przebiegu choroby Hashimoto. Jego obecność w diecie wpływa na spowalnianie procesów starzenia się. Biologiczne i toksykologiczne efekty działania selenu w znacznym stopniu zależą od jego postaci chemicznej. Niektóre organizmy, na przykład rośliny i drożdże, mają zdolność metabolizowania słabo przyswajalnych form selenu (nieorganiczny selen) do jego łatwo przyswajalnych związków (selen organiczny). Cel. Celem niniejszych badań było poznanie procesów biotransformacji selenu przez bakterie Lactobacillus pod kątem identyfikacji form chemicznych selenu. Materiały i metody. Badania specjacji selenu przeprowadzono z wykorzystaniem metody wysokosprawnej chromatografii cieczowej połączonej ze spektrometrią mas z plazmą indukcyjnie sprzężoną. Do ekstrakcji form chemicznych selenu z liofilizowanych bakterii użyto wody, mieszaniny lipazy i proteazy, a także lizozymu oraz SDS-u. Wyniki. Wszystkie badane szczepy bakterii, hodowane w obecności Na2SeO3, efektywnie pobierały selen. Żaden z zastosowanych ekstrahentów nie uwolnił jednak całego związanego w bakteriach selenu. Maksymalnie udało się wyekstrahować 10% pobranego przez bakterie selenu, przy zastosowaniu enzymów. Niemniej jednak, okazało się, że bakterie z rodzaju Lactobacillus są zdolne do metabolizowania nieorganicznych jonów selenu (IV) do Se-metioniny, Se-metyloselenocysteiny oraz niezidentyfikowanych form selenu. Wnioski. Badania potwierdziły zdolność bakterii probiotycznych do biotransformacji nieorganicznych form selenu do jego organicznych metabolitów. W związku z tym, wzbogacone w selen bakterie mogą być używane, jako dodatek do żywności funkcjonalnej.