Wyniki wyszukiwania - BazTech

1

Detection of two major cytochrome b lineages in pike-perch, Sander lucioperca, and first data on their distribution in European populations

Kohlmann K., Louati M., Kersten P., Bahri-Sfar L., Poulet N., Ben Hassine O. K.

Environmental Biotechnology

|

2013

|

Vol. 9, No. 1

1--5

EN

Despite of the growing interest in pike-perch for aquaculture and its economic importance in fisheries, knowledge on the population structure and phylogeography of the species is still limited. We report the discovery of two major cytochrome b lineages and describe a simple method for their detection based on PCR amplification followed by restriction digestion with Alw26I. Screening of 708 individuals showed that haplotype A was fixed or dominating in Central and East European countries, whereas haplotype B was mainly found in several French populations and Tunisian pike-perch introduced from Europe. Sequencing the complete cytochrome b cds of 17 representative individuals revealed that haplotypes A and B differed by five substitutions but also showed further differentiation of both haplotypes due to an additional substitution in a single haplotype A and B individual from France, respectively. Five partial pike-perch cytochrome b cds available from NCBI GenBank could also clearly be assigned to one or the other of the two major lineages. Therefore, this new mtDNA marker might be considered as suitable not only for studies on population structure and phylogeography of pikeperch but also to trace its introduction history and to assess the genetic composition of aquaculture brood stocks.

2

Badania próżniowego suszenia kości ryb w procesie produkcji mineralnych dodatków do żywności

Fatychov J. A., Kopeć A., Dutkiewicz D.

Postępy Techniki Przetwórstwa Spożywczego

|

2011

|

nr 1

60-65

PL

W przeprowadzonej pracy badawczej przedstawiono propozycję zarysu nowej technologii otrzymywania funkcjonalnych dodatków do żywności z kości ryb, stanowiących dotychczas odpad w procesie ich fi letowania. Dla realizacji badań procesu suszenia zbudowano doświadczalną suszarnię próżniową i opracowano metodykę przeprowadzania eksperymentu. Uzyskano dane dotyczące kinetyki suszenia kości z dorsza i sandacza, w zależności od różnych parametrów procesu. Czynnikami wpływającymi na przebieg procesu suszenia są temperatura płyt grzejnych i ciśnienie wewnątrz komory. Eksperyment określił zależność tych parametrów od szybkości (tempa) odwodnienia kości, o różnych zawartościach tłuszczu, w postaci wielomianu drugiego stopnia.

EN

The article presents the outline of the new technology of production of functional additives for food from fi sh bones, which are waste in the process of fi lleting yet. An experimental vacuum dryer and drying method was elaborated to carry out the experiment. Obtained data on the kinetics of drying the bones of the cod and perch, depending on various parameters of the process. Factors infl uencing the drying process are temperature of the heating plates located inside the dryer chamber and pressure into the chamber. Dependence of drainage rate of bone with various fat contents of these parameters in the form of second-degree polynomial was determined.

3

Application of nine species-specific microsatellite loci to characterize three pike-perch (Sander lucioperca) populations from the Aral Sea basin in Uzbekistan

Khurshut E., Kohlmann K.

Environmental Biotechnology

|

2009

|

Vol. 5, No. 1

3-10

EN

The pike-perch is one of the main commercial fish species in Uzbekistan. Historically, it inhabited the Aral Sea and deltas of inflowing rivers (Amu-Darya and Syr-Darya). At the beginning of the 1960s it was introduced into water bodies of the middle Amu-Darya and Syr-Darya from the Ural River. The present study was aimed to evaluate the current genetic status of wild pike-perch populations from different water bodies using nine recently isolated species-specific microsatellite markers. The three examined Uzbek pike-perch populations expressed significantly higher average numbers of alleles per locus (5.56 to 14.22) than three year-classes of a German wild population (3.11 to 3.78) included for comparison. On the other hand, average observed and expected heterozygosities were not significantly different in both regions (0.743 and 0.760 in Uzbek pike-perch; 0.576 and 0.471 in German pike-perch). After sequential Bonferroni corrections all Uzbek populations were found to be in Hardy-Weinberg equilibrium. Genetic differentiation between all populations as estimated by FST values was statistically significant. Pike-perch from Amu-Darya River delta and Tudakul reservoir were less differentiated from each other probably due to gene flow between them (both water bodies are connected) whereas the more distinct Syr-Darya River population showed indication for introgression with non-native individuals stocked in that region. Since stocking is widely practiced to satisfy the growing demand of pike-perch fisheries in Uzbekistan, fish seed for such activities should be obtained from local, genetically undisturbed populations such as the Amu-Darya River delta population.