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Nutrient Removal and Biomass Production by Culturing Saccharomyces Cerevisiae in Parboiled Rice Effluent

Fehrenbach Gustavo Waltzer, Sitowski Aline, Novo Diogo La Rosa, Mesko Márcia Foster, Gil de los Santos Diego, Leite Fábio Pereira Leivas

Ecological Engineering & Environmental Technology

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2022

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Vol. 23, iss. 3

177--183

EN

The objective of this study was to evaluate the potential of Saccharomyces cerevisiae in the treatment of rice parboiling effluent (PE) and biomass production. PE was inoculated with S. cerevisiae at 1.2×104 CFU mL-1 and cultured in shaker at 28 °C and 180 rpm for 72 h. PE supplied the required nutrients for S. cerevisiae growth, reaching a biomass of ± 8.2 g·L−1, cell viability of ± 2×1011 CFU mL−1 and removals of 74%, 56% and 17% for total Kjedahl nitrogen, chemical oxygen demand and phosphorus, respectively. The versatile of S. cerevisiae supported the direct and non-supplemented cultivation in PE, resulting in high removals of nutrient and biomass production and represent an alternative method to reduce the environmental impact of rice industry and an alternative process to obtain marketable yeast biomass.

2

Badanie mobilności wody w jabłkach odwadnianych osmotycznie metodą magnetycznego rezonansu jądrowego (NMR)®

Samborska K., Kowalska H., Tylewicz U., Dalla Rossa M.

Postępy Techniki Przetwórstwa Spożywczego

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2017

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Nr 2

20--26

PL

Celem prac przedstawionych w artykule jest analiza wpływu odwadniania osmotycznego (OD) na wymianę masy w jabłkach. W celu określenia zmian w strukturze oraz żywotności komórek użyto jądrowego rezonansu magnetycznego (NMR) oraz mikroskopu fluorescencyjnego. Odwadnianie osmotyczne prowadzono w roztworach sacharozy oraz mieszaninach sacharozy i soków owocowych o ekstrakcie 22 i 65°Brix w temperaturze 45°C. Analiza NMR może być wykorzystywana do określenia obecności i miejsca występowania wody w komórkach tkanki poddanej odwadnianiu osmotycznemu. Informacje te mogą być przydatne w ocenie zmian destrukcji tkanki i zaburzenia jej integralności, a od strony technologicznej do kontroli wymiany masy zachodzącej podczas odwadniania osmotycznego.

EN

The aim of this work presented in the paper is to analysis of the influence of osmotic dehydration (OD) on mass transfer in apples. Fluorescence microscopy and time domain nuclear magnetic resonance (NMR) were respectively used to evaluate cell viability and microstructural changes during osmotic dehydration. The OD of apple was carried out in 22 and 65°Brix sucrose solutions or mixture of sucrose solution and fruit juices at 45°C, for a contact period from 0 to 1440 min. NMR analysis can be used to determine the presence and water state in the dehydrated cells. This information may be useful in assessing changes in tissue destruction and its integrity, and on the technological side to control the mass exchange occurring OD.

3

Haemocompatibility and cytotoxic studies of non-metallic composite materials modified with magnetic nano and microparticles

Szymonowicz M., Rybak Z., Fraczek-Szczypta A., Paluch D., Rusak A., Nowicka K., Blazewicz M.

Acta of Bioengineering and Biomechanics

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2015

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Vol. 17, no. 3

49--58

EN

Purpose: Preventing the formation of blood clots on the surface of biomaterials and investigation of the reasons of their formation are the leading topics of the research and development of biomaterials for implants placed into the bloodstream. Biocompatibility and stability of a material in body fluids and direct effect on blood cell counts components are related both to the structure and physico-chemical state of an implant surface. The aim of this study was to determine haemocompatibility and cytotoxicity of polysulfone-based samples containing nano and micro particles of magnetite (Fe3O4). Methods: The polysulfone-based samples modified with nanometric and micrometric magnetite particles were examined. Physicochemical properties of the composites were determined by testing their wettability and surface roughness. The action of haemolytic, activation of coagulation system and cytotoxicity of composites was evaluated. Results: Wettability and roughness of materials were correlated with nanoparticles and microparticles content. In the tests of plasma coagulation system shortening of activated partial thromboplastin time for polysulfone with nano magnetite and with micro magnetite particles was observed in comparison with pure polysulfone. Prothrombine time and thrombine time values as well as fibrinogen concentration were unchanged. Haemolysis values were normal. Morphology and viability of cells were normal. Conclusions: Composites made from polysulfone modified with nanoparticles and microparticles of magnetite cause neither haemolytic nor cytotoxic reaction. These composites evoke plasma endogenous system activation.

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Synthesis and characterization of nanostructured CaSiO3 biomaterial

Jagadale P N, Kulal S R, Joshi M G, Jagtap P P, Khetre S M, Bamane S R

Materials Science Poland

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2013

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Vol. 31, No. 2

269--275

EN

Here we report a successful preparation of nanostructured calcium silicate by wet chemical approach. The synthesized sample was characterized by various physico-chemical methods. Thermal stability was investigated using thermo-gravimetric and differential thermal analysis (TG-DTA). Structural characterization of the sample was carried out by the X-ray diffraction technique (XRD) which confirmed its single phase hexagonal structure. Transmission electron microscopy (TEM) was used to study the nanostructure of the ceramics while homogeneous grain distribution was revealed by scanning electron microscopy studies (SEM). The elemental analysis data obtained from energy dispersive X-ray spectroscopy (EDAX) were in close agreement with the starting composition used for the synthesis. Superhydrophilic nature of CaSiO3 was investigated at room temperature by sessile drop technique. Effect of porous nanosized CaSiO3 on early adhesion and proliferation of human bone marrow mesenchymal stem cells (BMMSCs) and cord blood mesenchymal stem (CBMSCs) cells was measured in vitro. MTT cytotoxicity test and cell adhesion test showed that the material had good biocompatibility and promoted cell viability and cell proliferation. It has been stated that the cell viability and proliferation are significantly affected by time and concentration of CaSiO3. These findings indicate that the CaSiO3 ceramics has good biocompatibility and that it is promising as a biomaterial.

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Komórkowe efekty oddziaływania wolnozmiennych pól magnetycznych

Sieroń A., Kawczyk-Krupka A.

Acta Bio-Optica et Informatica Medica. Inżynieria Biomedyczna

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1998

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Vol. 4, nr 3

79-85

PL

Prace eksperymentalne na modelu komórkowym wykazują różnorodne działanie biologiczne wolnozmiennych pól magnetycznych, zależnie od parametrów fizycznych pól: indukcji, częstotliwości, kształtu fali oraz od czasu ekspozycji. Wolnozmienne pola magnetyczne modyfikują różnorodne funkcje życiowe, ekskrecję, podziały komórkowe, a także ekspresje genów. Eksperymenty przeprowadzane na hodowlach komórkowych pozwalają na obiektywną ocenę efektu działania wolnozmiennych pól magnetycznych na struktury subkomórkowe.

EN

Experimental studies at the cellular level have demonstrated various biological effects of the magnetic fields. Studies with cellular systems using different exposure regimes durations, amplitudes, frequencies and waveforms indicate, that biological effects of magnetic fields on cellular systems may be related to cell viability, multiplication, gene expression, cellular excretion, or even tumour promotion. The experiments performed on tissue cultures seem to give better insight into the effects evoked by magnetic field within a single cell and its subcellular organelles.