Effect of Heavy Metals (Cu and Zn) on the Content of Photosynthetic Pigments in the Cells of Algae Chlorella vulgaris

• Autorzy: Kondzior P. , Butarewicz A.

Identyfikatory

DOI

10.12911/22998993/85375

• Języki publikacji: EN

Abstrakty

EN

The purpose of this paper is to determine the effect of heavy metals on the photosynthetic pigments of chlorophyll a, b and the carotenoids found in the cells of algae Chlorella vulgaris. In order to analyze the influence of heavy metals on Chlorella vulgaris algae, two heavy metals were chosen, i.e. copper and zinc. The samples for analysis were collected daily for 7 days. Copper(II) sulphate, which is widely regarded as an algicide, was used in the study. Chlorella vulgaris grows at the highest concentration tested, amounting to 0.15 mg CuSO₄/dm³. In the trial with a concentration of 0.15 mg CuSO₄/dm³, a decrease in the content of chlorophyll was observed, which was lower by 63% in comparison to the control sample, 7 days after incubation was observed. In the second study using zinc(II) sulphate at a concentration of 100 mg ZnSO₄/dm³, the death of Chlorella vulgaris was observed after 5 days of incubation. In subsequent tests with lower concentrations of zinc(II) sulphate, Chlorella vulgaris is growing, but with lesser dynamics than in the case of the control sample. The increase in the content of photosynthetic pigments, along with the incubation time, indicates the development of algae breeding. The conducted research shows that the Chlorella vulgaris algae has a specific resistance to the presence of the elevated content of tested heavy metals in the breeding medium.

Słowa kluczowe

EN

heavy metals; photosynthetic pigments; copper; zinc; algae; Chlorella vulgaris

• Wydawca: Polskie Towarzystwo Inżynierii Ekologicznej ; Lublin University of Technology
• Czasopismo: Journal of Ecological Engineering
• Rocznik: 2018
• Tom: Vol. 19, nr 3
• Strony: 18--28
• Opis fizyczny: Bibliogr. 20 poz., tab., rys.

Twórcy

autor

Kondzior P.

• Department of Chemistry, Biology and Biotechnology, Faculty of Civil and Environmental Engineering, Bialystok University of Technology, ul. Wiejska 45 E, 15-351 Bialystok, Poland

autor

Butarewicz A.

• Department of Chemistry, Biology and Biotechnology, Faculty of Civil and Environmental Engineering, Bialystok University of Technology, ul. Wiejska 45 E, 15-351 Bialystok, Poland

Bibliografia

• 1. Bajguz A. 2007. Effect of brassinosteroids on algae cultures Chlorella vulgaris treated with selected phytohormones and stress factors. Publisher of the University of Bialystok (In Polish).
• 2. Bajguz A. 2010. An enhancing effect of exogenous brassinolide on the growth and antioxidant activity in Chlorella vulgaris cultures under heavy metals stress. Environmental and Experimental Botany, Vol. 68, 175–179.
• 3. Bajguz A. 2011. Suppression of Chlorella vulgaris Growth by Cadmium, Lead, and Copper Stress and Its Restoration by Endogenous Brassinolide. Arch Environ Contam Toxicol, Vol. 60, 406–416.
• 4. Bandurska H. 2007. Mineral nutrition of plants (In Polish) [IN:] Plant physiology. Kozłowska M. [ed.], Państwowe Wydawnictwo Rolnicze i Leśne, Poznań.
• 5. Bulgariu L., Gavrilescu M. 2015. Bioremediation of Heavy Metals by Microalgae [IN:] Handbook of marine microalgae biotechnology advances. Kim S-K. [ed.], 457–469.
• 6. Czaplicka-Kotas A. (2007). Studies on the impact of water quality on the production of photosynthetic pigments in Chlorella vulgaris algae for the needs of surface water biomonitoring (In Polish). Ochrona środowiska. 29(1).
• 7. Gabryś H., Kacperska A., Kopcewicz J., Lewak S., Starck Z., Strzałka K., Tretyn A. 2005. Plant physiology (In Polish) Kopcewicz i Lewaka [ed.], PWN Publishing House, Warsaw.
• 8. Hochmuth D. J., Asselman J., Schamphelare K.A.C. 2014. Are interactive effects of harmful algal blooms and copper pollution a concern for water quality management? Water Research, Vol. 60, 41–53.
• 9. Hou J., Wu Y., Li X., Wei B., Li S., Wang X. 2018. Toxic effects of different types of zinc oxide nanoparticles on algae, plants, invertebrates, vertebrates and microorganisms. Chemosphere, Vol. 193, 852–860.
• 10. Kondzior P. 2017. The use of algae and cyanobacteria in engineering and environmental protection (In Polish), [IN:] Monographs Inżynieria Środowiska – Młodym Okiem, Vol. 32, 65–81, Publishing house of the Bialystok University of Technology, Bialystok.
• 11. Mallick N. 2004. Copper-induced oxidative stress in the chlorophycean microalga Chlorella vulgaris: response of the antioxidant system. Journal of Plant Physiology, Vol. 161, 591–597.
• 12. Paniagua-Michel J. 2015. Bioremediation with Microalgae: Toward Sustainable Production of Biofuels [IN:] Handbook of marine microalgae biotechnology advances. Kim S-K. [ed.], 471–482.
• 13. Piotrowska-Niczyporuk A., Bajguz A., Zambrzycka E., Godlewska-Żyłkiewicz B. 2012. Phytohormones as regulators of heavy metal biosorption and toxicity in green alga Chlorella vulgaris (Chlorophyceae). Plant Physiology and Biochemistry, Vol. 52, 52–65.
• 14. Piotrowska-Niczyporuk, A., Bajguz, A. 2015. The share of algae in the phytoremadation of water environments contaminated with heavy metals. Biodiversity – from the cell to the ecosystem. Functioning of plants and fungi Environment-experiment-learning (In Polish). Bajguz A. and Ciereszko I. [ed.], 239–252.
• 15. Pozdniakova T.A., Mazur L.P., Boaventura R.A.R., Vilar V.J.P. 2016. Brown macro-algae as natural cation exchangers for the treatment of zinc containing wastewaters generated in the galvanizing process. Journal of Cleaner Production, Vol. 119, 38–49.
• 16. Szczukocki D., Krawczyk B., Czarny K. Romanowska-Duda Z. (2015). Toxicity tests using green algae of the genus Chlorella sp. and Scenedesmus armatus. Technology of growing microalgae in closed bioreactors with CO2 recycling and other biogas wastes (In Polish), Szwaja S. [ed.], Wydawnictwo Instytut Maszyn Cieplnych Politechniki Częstochowskiej, Częstochowa, 287–298.
• 17. Wan Maznah W.O., Al-Fawwaz A.T., Surif M. 2012. Biosorption of copper and zinc by immobilised and free algal biomass, and the effects of metal biosorption on the growth and cellular structure of Chlorella sp. and Chlamydomonas sp. isolated from rivers in Penang, Malaysia. Journal of Environmental Sciences, 24(8), 1386–1393.
• 18. Węglarzy K. 2007. Heavy metals – the source of pollution and the effect on the environment (In Polish). Wiadomości Zootechniczne, XLV(3), 31–38.
• 19. Xiong, J.-Q., Kurade, M.B., Abou-Shanab, R.A.I., Ji, M.-K., Choi, J., Oh Kim, J., Jeon, B.-H. 2016. Biodegradation of carbamazepine using freshwater microalgae Chlamydomonas mexicana and Scenedesmus obliquus and the determination of its metabolic fate. Bioresource Technology, 205, 183–190.
• 20. Zeraatkar A. K., Ahmadzadeh H., Talebi A. F., Moheimani N. R., McHenry M. P. 2016. Potential use of algae for heavy metal bioremediation, a critical review. Journal of Environmental Management, Vol. 181, 817–831.

Uwagi

PL

Opracowanie rekordu w ramach umowy 509/P-DUN/2018 ze środków MNiSW przeznaczonych na działalność upowszechniającą naukę (2018).