Wyniki wyszukiwania - Biblioteka Nauki

1

Diacylglycerol signalling: targeting protein kinase C isozymes and 'non-kinase' diacylglycerol effectors

100%

Kazanietz M. G.

Cellular and Molecular Biology Letters

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2005

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tom 10

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nr Suppl.2

2

Alterations in the lactate dehydrogenase isoenzyme pattern after maximal running exercise

100%

Szade B. , Poprzecki S. , Klapcinska B. , Sadowska-Krepa E. , Grzesiok K. , Iskra J. , Sobiech K. A.

Polish Journal of Environmental Studies

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1998

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tom 07

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nr 6

3

Induced isozyme polymorphism in spring barley mutants

88%

Kucharska M. , Maluszynski M.

Journal of Applied Genetics

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1996

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tom 37

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nr 1

EN

The usefulness of mutagenic treatment to enlarge isozymic variability of barley and the use of induced mutants for genetic analysis were evaluated. N-methyl-N-nitroso urea, sodium azide and gamma rays were employed as mutagenic agents. Electrophoretic assays of 3848 M₂ seedlings obtained by chemical mutagenic treatment of the spring barley cultivars Dema, Aramir, Bielik and 3100 M₂ seedlings obtained by physical mutagenic treatment of the cv. Dema revealed 70 isozymic mutants, which represent 30 separate mutants in 25 M₁ plants. Most of mutations (27) were induced by chemical mutagen at polymorphic esterase loci. The occurrence of induced mutants at monomorphic loci, Got2 and Lap2, made it possible to perform genetic analysis of those loci in barley including mapping respective genes within chromosomes.

4

The expression of GST isoenzymes and p53 in non-small cell lung cancer

75%

Oguztuzun S. , Aydin M. , Demirag F. , Yazici U. , Ozhavzali M. , Kilic M. , Iscan M.

Folia Histochemica et Cytobiologica

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2010

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tom 48

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nr 1

122-127

5

Immunochemical comparison of the glutamate dehydrogenase isoenzymes in yellow lupin root nodules

75%

Ratajczak L. , Prus-Glowacki W. , Ratajczak W. , Lehmann T.

Acta Biochimica Polonica

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1989

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tom 36

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nr 3-4

6

The alcohol dehydrogenase isoenzyme (ADH IV) as a candidate tumour marker of esophageal cancer

75%

Jelski W. , Laniewska-Dunaj M. , Niklinski J. , Kozlowski M. , Laudanski J. , Szmitkowski M.

Acta Biochimica Polonica

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2013

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tom 60

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nr 3

EN

Objective: Alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) are present in esophageal cancer cells. Moreover the total activity of ADH as well as the activity of class IV ADH isoenzyme is significantly higher in cancer tissue than in healthy mucosa. The activity of these enzymes in cancer cells is reflected in the sera and could thus be helpful for diagnostics of esophageal cancer. The aim of this study was to investigate a potential significance of ADH isoenzymes and ALDH as tumour markers of esophageal cancer. We defined diagnostic sensitivity, specificity, predictive value for positive and negative results, and receiver-operating characteristics (ROC) curve for tested enzymes. Methods: Serum samples were taken for routine biochemical investigation from 180 patients with esophageal cancer before treatment. Total ADH activity was measured by a photometric method with p-nitrosodimethylaniline as a substrate and ALDH activity by a fluorometric method with 6-methoxy-2-naphtaldehyde as a substrate. For the measurement of the activity of class I and II isoenzymes we employed the fluorometric methods, with class-specific fluorogenic substrates. The activity of class III alcohol dehydrogenase was measured by a photometric method with formaldehyde and class IV with m-nitrobenzaldehyde as a substrate. Results: There was a significant increase in the activity of class IV of ADH isoenzyme (7.65 mU/l vs 5.88 mU/l) and total ADH activity (1198 mU/l vs 848 mU/l) in the sera of esophageal cancer patients compared to the control. The diagnostic sensitivity for ADH IV was 72%, the specificity 76%, the positive and negative predictive values were 80% and 72% respectively. The area under the ROC curve for ADH IV was 0.65. Conclusion: The results suggest a potential significance of ADH IV as a marker of esophageal cancer.

7

Cytosolic purine 5'-nucleotidase from chicken heart: an isozyme of the liver enzyme as evidenced by northern blot analysis

75%

Oka J.

Cellular and Molecular Biology Letters

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1999

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tom 04

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nr 3

8

Genetic protein variability in tench [ Tinca tinca L.] stocks in Czech Republic

75%

Slechtova V. , Slechta V. , Valenta M.

Polskie Archiwum Hydrobiologii

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1995

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tom 42

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nr 1-2

9

Further studies on the role of phospholipids in determining the characteristics of mitochondrial binding sites for type I hexokinase

75%

Hutny J. , Wilson J. E.

Acta Biochimica Polonica

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2000

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tom 47

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nr 4

EN

Previous work has indicated that two types (A and B) of binding sites for hexokinase exist, but in different proportions, on brain mitochondria from various species. Hexokinase is readily solubilized from Type A sites by glucose 6-phosphate (Glc-6-P), while hexokinase bound to Type B sites remains bound even in the presence of Glc-6-P. Type A:Type B ratios are approximately 90:10, 60:40, 40:60, and 20:80 for brain mitochondria from rat, rabbit, bovine and human brain, respectively. The present study has indicated that MgCl2-dependent partitioning of mitochondrially bound hexokinase into a hydrophobic (Triton X-114) phase is generally correlated with the proportion of Type B sites. This partitioning behavior is sensitive to phospholipase C, implying that the factor(s) responsible for conferring hydrophobic character is(are) phospholipid(s). Substantial differences were also seen in the resistance of hexokinase, bound to brain mitochondria from various species, to solubilization by Triton X-100, Triton X-114, or digitonin. This resistance increased with proportion of Type B sites. Enrichment of bovine brain mitochondria in acidic phospholipids (phosphatidylserine or phosphatidylinositol), but not phosphatidylcholine or phosphatidylethanolamine, substantially increased solubilization of the enzyme after incubation at 37°C. Collectively, the results imply that the Type A and Type B sites are located in membrane domains of different lipid composition, the Type A sites being in domains enriched in acidic phospholipids which lead to greater susceptibility to solubilisation by Glc-6-P.

10

Inheritance of seed beta-amylases in Pisum

75%

Zimniak-Przybylska Z.

Genetica Polonica

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1992

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tom 33

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nr 3

11

Genetic analysis of erythrocyte AMP deaminase deficiency

75%

Ogasawara N. , Yamada Y. , Goto H.

Cellular and Molecular Biology Letters

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1999

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tom 04

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nr 3

12

Genetic diversity test of re-established population of Allium angulosum L.

75%

Zeidler M. , Curn V.

Polish Journal of Ecology

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2003

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tom 51

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nr 1

EN

The genetic diversity of re-established population of endangered species Allium angulosum L. was tested as a one part of rescue program. Founder individuals were picked in Chropyně - Záříčí area (North Moravia, Czech Republic) and new population was set in Protected Landscape Area Litovelské Pomoravi (North Moravia, Czech Republic). The task was whether the newly founded population was made by representative individuals to cover (include) the genetic variability of source (mother) population. Items were tested with variability assay of six isozyme systems (G-6-PDH, AAT, PGM, EST, ACP, PGI) using discontinuous polyacrylamide gel electrophoresis (PAGE). The method stated relatively sufficient level of variability on condition that new population would be raised to prevent genetic changes. Application of more tests checking the genetic diversity within population could be useful during reintroduction and management of endangered plant species.

13

Using enzyme polymorphism to identify the gametic origin of flax regenerants

75%

Bartosova Z. , Obert B. , Takac T. , Kormutak A. , Pretova A.

Acta Biologica Cracoviensia. Series Botanica

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2005

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tom 47

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nr 1

EN

Anther culture is currently the most successful method for production of doubled haploid lines in flax. Recently, ovary culture was also described as a good source of doubled haploids. In this contribution we investigated the incorporation of enzyme polymorphism of acid phosphatase and peroxidase as molecular markers for the gametic origin of flax plants derived from anther and ovary cultures.

14

Different isoenzyme patterns of nonspecific esterases and the level of IL6 production as markers of macrophage functions

75%

Czajkowska B. , Ptak M. , Bobek M. , Bryniarski K. , Szczepanik M.

Folia Histochemica et Cytobiologica

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1995

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tom 33

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nr 2

15

Isoenzymes of N-acetyl-beta-hexosaminidase in complicated pregnancy

75%

Arciuch L. P. , Bielecki D. , Borzym M. , Poludniewski G. , Arciszewski K. , Rozanski A. , Zwierz K.

Acta Biochimica Polonica

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1999

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tom 46

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nr 4

EN

The activity of N-acetyl-β-hexosaminidase was found to be significantly higher in the placentas collected after delivery from women in puerperium with symptoms of prolonged pregnancy or complicated by EPH gestosis, than in placentas from normal pregnancy. Isoelectrofocusing of placenta homogenates showed the presence of isoenzymes A, P and B of N-acetyl-β-hexosaminidase. Different isoenzyme A patterns in homogenates were observed in placentas obtained from normal and prolonged pregnancies and in those complicated by EPH gestosis.

16

The activity of monoamine oxidase and its isoenzymes A and B in bovine blood serum near the parturition

75%

Jaroszewski J. J. , Markiewicz W. , Dynarowicz I. , Janowski T. , Zdunczyk S. , Majewski M.

Polish Journal of Veterinary Sciences

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2000

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tom 03

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nr 3

EN

The activity of monoamine oxidase (MAO) in cow serum undergoes considerable fluctuations in the course of the oestrous cycle. The aim of the study was to determine the activity of MAO and its isoenzymes A (MAO-A) and B (MAO-B) in cow serum near the parturition. The experiments were performed on 8 pregnant and clinically healthy cows of the Polish Black and White Breed. Peripheral blood samples were collected every 4 hours before, during and after parturition. MAO serum activity was determined with the use of the spectrofluorimetric technique according to Matsumoto et al. (1985) with own modifications. Analysis of serum samples showed that MAO activity increased rapidly near the delivery. MAO-B activity was significantly higher than MAO-A activity and determined the profile of total MAO (MAO-T) activity. It may be suggested that an increase in MAO activity observed near delivery protects against an excessive elevation of catecholamine concentration in blood. However, the control mechanism and physiological role of MAO during this period need further investigations.

17

Isozyme markers, cryptic species and allopolyploidy in bryophytes

75%

Odrzykoski I. J.

Biological Bulletin of Poznań

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2001

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tom 38

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nr 2

18

L-alanine:2-oxoglutarate aminotransferase isoenzymes from Arabidopsis thaliana leaves

75%

Wisniewski P. , Szklarczyk J. , Maciaga M. , Paszkowski A.

Acta Physiologiae Plantarum

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2006

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tom 28

|

nr 6

EN

The occurrence of four L-alanine:2-oxoglutarate aminotransferase (AOAT) isoenzymes (AOAT-like proyeins): alanine aminotransferase 1 and 2 (AlaATl and AlaAT2, EC 2.6.1.2) and L-glutamate:glyoxylate aminotransferase 1 and 2 (GGAT1 and GGAT2, EC 2.6.1.4) was demonstrated in Arabidopsis thaliana leaves. These enzymes differed in their substrate specificity, susceptibility to pyridoxal phosphate inhibitors and behaviour during molecular sieving on Zorbax SE-250 column. A difference was observed in the electrostatic charge values at pH 9.1 between GGAT1 and GGAT2 as well as between AlaAT1 and AlaAT2, despite high levels of amino acid sequence identity (93 % and 85 %, respectively). The unprecedented evidence for the monomeric structure of both AlaAT1 and AlaAT2 is presented. The molecular mass of each enzyme estimated by molecular sieving on Sephadex G-150 and Zorbax SE-250 columns and SDS/PAGE was approx-mately 60 kDa. The kinetic parameters: Km (Ala) = 1.53 mM, Km (2-oxoglutarate) = 0.18 mM, kcat = 124.6 s⁻¹, kcat/Km = 8.1 x 10⁴ M⁻¹-s⁻¹ of AlaAT1 were comparable to those determined for other AlaATs iso-ated from different sources. The two studied GGATs also consisted of a single subunit with molecular mass of 47.3-70 kDa. The estimated Km values for L-glutamate (1.2 mM) and glyoxylate (0.42 mM) in the transamination catalyzed by putative GGAT1 contributed to indentification of the enzyme. Based on these results we concluded that each of four AOAT genes in Arabidopsis thaliana leaves expresses different AOAT isoenzyme, functioning in a native state as a monomer.

19

Purification and properties of alpha-galactosidase isozymes from Phlebia radiata

75%

Prendecka M. , Szyjka K. , Rogalski J.

Acta Microbiologica Polonica

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2003

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tom 52

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nr 1

20

Proposal for a seed certification scheme

75%

Behm A. , Konnert M.

Dendrobiology

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2002

|

tom 47 Supplement

EN

Identity of reproductive material is essential in artificial forest regeneration. Legal regulations cannot guarantee proof of identity. State and private organizations have been cooperating to develop a seed certification scheme in forestry. Representative samples of forest reproductive material are taken at any production stage starting at the time of seed collection through to plant delivery as well as at time of changing ownership.The first sample at the time of seed harvest is used to determine the maximum potential yield of living germinants from the total collection. Furhtermore this and the other samples are tested randomly at a determined level of intensity or in case of doubt by Isozyme/DNA methods. Sampling techniques have been tested and described in a handbook. The biochemical-genetic tests are being standardized for all major commercial species. Forest reproductive material having been sampled in the described way will be certified "of provable identity" by a neutral agency. The Scheme will be open to anyone complying with the rules laid down. Larger forest owners have confirmed to preferably purchase "forest plants of provable identity".