Wyniki wyszukiwania - Biblioteka Nauki

1

Kontrowersje wokół bezpieczeństwa diet wysokobiałkowych

100%

Kwasniewska A.

Bromatologia i Chemia Toksykologiczna

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2011

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tom 44

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nr 3

2

Infekcje wirusem brodawczaka ludzkiego [HPV - Human Papillomavirus], surowiczy poziom antyoksydantow oraz rola zywienia w dysplazji szyjki macicy

100%

Kwasniewska A.

Seria Biologiczna. Uniwersytet im. Adama Mickiewicza w Poznaniu

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1998

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nr 61

199

3

Rola komórek T regulatorowych w ciąży

63%

Kwiatek M. , Kwasniewska A.

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tom 38

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nr 3

4

Poziom retinolu w surowicy krwi kobiet zakazonych HPV i ze zmianami dysplastycznymi szyjki macicy

63%

Kwasniewska A. , Tukendorf A.

Medycyna Doświadczalna i Mikrobiologia

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1996

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tom 48

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nr 1-2

71-77

5

Prevalence of human papillomavirus in high grade dysplasia and squamous cell cervical cancer in women in Norway

63%

Pochylski T. , Kwasniewska A.

|

2003

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tom 58

6

Prevalence of human papillomavirus [HPV] and herpes simplex virus type 2 [HSV-2] in cervical dysplasias and carcinomas

63%

Pochylski T. , Kwasniewska A.

Bulletin of the Veterinary Institute in Puławy. Supplement

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2002

7

Czestosc infekcji HPV oraz poziom kwasu askorbinowego w surowicy krwi kobiet z patologia szyjki macicy

51%

Kwasniewska A. , Tukendorf A. , Semczuk M.

Medycyna Doświadczalna i Mikrobiologia

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1996

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tom 48

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nr 3-4

183-188

8

Analysis of expression of AIF and PARP-1 in cancerogenesis of HPV-positive cervix

51%

Warowicka A. , Kwasniewska A. , Sobieraj M. , Gozdzicka-Jozefiak A.

Bulletin of the Veterinary Institute in Puławy

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2011

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tom 55

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nr 3

EN

The mRNA expression of AIF and PARP-1 in HPV negative non-tumour epithelial cells and HPV-positive cervical cancer cells using the real-time PCR method was examined. An increased level of AIF and PARP-1 mRNA in cervical cancer cells in comparison to normal epithelial cells was demonstrated. These results suggest that changes in the mRNA expression level of AIF and PARP-1 might be involved in cervical cancer development. The analysis of these two factors may represent a new molecular tool for cervical cancer prevention in women with HPV persistent infection.

9

Pojedyncza tętnica pępowinowa u płodu

51%

Geca T. , Kwiatek M. , Krzyzanowski A. , Kwasniewska A.

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nr 4

10

Komórki macierzyste w naprawie urazów rdzenia kręgowego - aktualny stan wiedzy

51%

Zawadzka M. , Kwasniewska A. , Miazga K. , Slawinska U.

Wszechświat

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2020

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tom 121

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nr 01-03

11

Szkoła rodzenia - profil pacjentek oraz wpływ zajęć na przebieg porodu i stan noworodka

51%

Kwiatek M. , Geca T. , Biegaj-Fic J. , Kwasniewska A.

Medycyna Ogólna i Nauki o Zdrowiu

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2011

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tom 17(46)

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nr 3

12

Izolacja, identyfikacja i ocena lekooporności gronkowców w powietrzu domu studenckiego Uniwersytetu Rolniczego w Krakowie

51%

Lenart-Boron A. , Wolny-Koladka K. , Kwasniewska A.

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nr 16

PL

Celem przedstawionej pracy była izolacja, identyfikacja gatunkowa oraz ocena lekooporności bakterii z rodzaju Staphylococcus,wyizolowanych z powietrza domu studenckiego Uniwersytetu Rolniczego w Krakowie. Pobór próbek powietrza był wykonywany przy użyciu próbnika MAS-100 (Merck), z zastosowaniem podłoża Chapmana. Po uzyskaniu czystych izolatów bakterii przeprowadzono identyfikację gatunkową przy użyciu m.in. posiewów na podłoża chromogenne i testów biochemicznych. Badania lekooporności wykonano w oparciu o zalecenia Krajowego Ośrodka Referencyjnego ds. Lekowrażliwości Drobnoustrojów (KORLD). Pozyskano 10 izolatów należących do 5 gatunków: S. xylosus (2), S. epidermidis(1), S. cohniiurealyticum(5), S. aureus(1), S. haemolyticus(1). Wszystkie izolaty, poza S. aureus, były koagulazoujemne oraz dały negatywny wynik w teście na obecność białka A. Wszystkie izolaty charakteryzowały się wrażliwością na metycylinę, natomiast wśród antybiotyków z grupy makrolidów i linkosamidów wyróżniono różne fenotypy oporności. Stwierdzono, że zanieczyszczenie powietrza gronkowcami w domu studenckim nie stwarza bezpośredniego niebezpieczeństwa dla osób tam przebywających, a wyizolowane gronkowce to głównie gatunki należące do mikroflory skóry człowieka.

EN

The aim of this study was the isolation, identification and evaluation of the drug-resistance of Staphylococcusspp., isolated from the air in a dormitory of the University of Agriculture in Cracow. Air sampling was performed using the MAS-100 sampler (Merck), using the Chapman medium. After collection of isolates, bacterial species were identified using cultures on chromogenic media and biochemical tests. The drug-resistance evaluation was performed based on the recommendations of the National Reference Centre for Antimicrobial Susceptibility (KORLD). The analyzed 10 isolates belonged to 5 species: S. cohnii urealyticum(5), S. xylosus (2), S. epidermidis(1), S. aureus(1), S. haemolyticus(1). All isolates except S. aureuswere coagulase-negative and gave a negative result in the test for the protein A. All isolates were susceptible to methicillin, whereas among the group of macrolide antibiotics and lincosamides different resistance phenotypes were distinguished. It was concluded that the air contamination with Staphylococcusspp. in a dormitory was not a direct threat to its residents, and the isolated staphylococci belong mainly to the human skin microflora.

13

Expression of IGF1, IGF1R, and SP1 factors in HPV infected cervical cancer cells

51%

Koczorowska M. M. , Kwasniewska A. , Gozdzicka-Jozefiak A.

Bulletin of the Veterinary Institute in Puławy

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2011

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tom 55

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nr 2

EN

The aim of the study was to investigate the mRNA expression levels of IGF1, IGF1R, and SP1 in cervical epithelial cells in different stages of cervical cancerogenesis using real-time PCR. The analysis indicated up-regulation of IGF1 gene expression in L- SIL and H-SIL, followed by its down-regulation in cervical cancer. Firstly, the obtained results suggest that IGF1 can be recognised asan essential factor in cervical cancerogenesis; secondly the SP1 action may be crucial for IGF1 expression.

14

Content of beta-carotene in blood serum of human papillomavirus infected women with cervical dysplasias

51%

Kwasniewska A. , Tukendorf A. , Semczuk M.

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nr 5-6

15

Proliferation of cells and expression of rars, rxrs and hpv viral e6 and e7 proteins in cervical cancer cell lines after treatment with atra

45%

Myga-Nowak M. , Pacholska-Bogalska J. , Kwasniewski W. , Kwasniewska A. , Gozdzicka-Jozefiak A.

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tom 18

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nr 1

16

Study on RAR and RXR receptors in HPV16/18 positive cervical cancer cells

45%

Myga M. , Parol I. , Kedzia W. , Kwasniewska A. , Gozdzicka-Jozefiak A.

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nr Supplement 1

17

Identification of point mutation in c-FOS gene from displastic cells and squamous cell cervical neoplasms

45%

Kwasniewska A. , Gozdzicka-Jozefiak A. , Skoczynski M. , Kuzma D. , Pochylski T.

Polish Journal of Environmental Studies

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2004

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tom 13

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nr Suppl.2, Part 1

EN

Human Papillomavirus (HPV) is one of the DNA viruses which are closely related to cervical carcinogenesis. c-FOS plays a key role in initiation and maintenance of expression of HPV E6 and E7 oncoproteins in cervical carcinogenesis combined with infection with oncogenic types of HPV. AP-1 is considered to be a positive regulator which recognizes sequences in 5'HPV DNA regulatory region (LCR). Mutations in FOS gene, nuclear transcription factor coding proteins binding to specific DNA sequences occur with a frequency of 20-25% in various neoplasms in humans. The purpose of the study was identification of point mutation in c-FOS gene from cervical squamous carcinoma cells, high grade cervical intraepithelial neoplasia cells and normal cervical epithelium.The study group consisted of 35 postoperative tissues from patients diagnosed with high grade dysplasia and 29 postoperative tissues from patients diagnosed with squamous cell cervical carcinoma. The control group consisted of normal cervical tissue specimens obtained from 33 patients that underwent hysterectomy due to uterine leiomyomas. Identification of point mutation in c-FOS gene exon 4 was performed using PCR - SSCP technique. Mutation in 450 nucleotide fragment of c-FOS gene was found in none of the studied samples.

18

Prevalence of Chlamydia trachomatis in cervical and vulvar carcinoma of the Lublin Region patients

45%

Kwasniewska A. , Skoczynski M. , Korobowicz E. , Semczuk M. , Gozdzicka-Jozefiak A.

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tom 51

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nr 3

EN

The aim of the study was to evaluate frequency of occurrence of Chlamydia trachomatis infection in samples of cervical and vulvar cancer in patients of the Lublin Region. The study was performed on paraffin sections prepared from the specimens of cervical cancer obtained from 570 patients and of vulvar cancer from 46 patients. We identified archival diagnostic phase tissue specimens. The control material to that obtained from patients with cervical cancer consisted of normal cervical tissues. The control material to that obtained from patients with vulvar cancer were fragments of normal epithelial tissue collected from the same paraffin blocks containing material from the margin of surgical section during vulvectomy. In order to identify Chlamydia trachomatis, DNA isolated from archival material was analysed and PCR was performed using starters complementary to Chlamydia trachomatis. Statistically significantly higher frequency of the occurrence of Chlamydia trachomatis was observed in sections from patients with invasive cervical cancer compared to control group. In the analysed material, the frequency of cases of vulvar cancer with co-occurrence of Chlamydia trachomatis infection was not statistically significant. Chlamydia trachomatis may not be directly involved in the oncogenic processes but may enhance the possibility of oncogenesis or infect cancer tissues opportunistically.

19

Quantity examination of E6 and E7 proteins of HPV 18 in HeLa cell line incubated with retinoid acid

45%

Dziubinska-Parol I. , Gasowska U. , Rzymowska J. , Wojcierowski J. , Kwasniewska A.

Bulletin of the Veterinary Institute in Puławy. Supplement

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2002

20

Influence of 17beta-estradiol on survival and proliferation of HeLa line cells

38%

Dziubinska-Parol I. , Rzymowska J. , Myga M. , Parol W. , Gozdzicka-Jozefiak A. , Kwasniewska A.

Polish Journal of Environmental Studies

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2004

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tom 13

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nr Suppl.2, Part 1

EN

Objectives: Molecular mechanism of carcinogenesis associated with high risk (HR) type HPV is related to the activity of virus oncoproteins E5, E6 and E7. Their task is to bring the cells to a state enabling synthesis of viral DNA, copying viral particles and promotion of uncontrolled cell growth. Proliferative factors of a feminine genital tract epithelium are also sex hormones — estradiol and progesterone. Steroids influence the transcription of genes involved in cell cycle, acting through specific nuclear receptors (ER and PR). The aim of this study was evaluation of the effect of selected concentrations of 17ß-estradiol and progesterone on survival and proliferation of HeLa line cells. Material and methods: The study was done on a transformed HeLa cell line containing integrated genome HPV of type 18. The lines were incubated in the presence of 17ß-estradiol at the concentrations of lx10-4M, lx10-5M, lx10-7M, lx10-8M. Cell survival was determined with the use of 0.5% of water solution of toluidine blue and the cell proliferation rate were evaluated with the use of BrdU Labelling and Detection Kit and the method ELISA (Roche). Results: The obtained results point to 10% toxic influence on HeLa line cells of 17ß-estradiol at high concentrations after 72 h of incubation. The influence of the hormone on proliferation rate was diversified depending on the hormone concentration and time.