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EN
The aim of our study was to evaluate the genetic relationship of Rhododendrons from Rhododendron collection at Tomaszkowice in Pogórze Wielickie. Leaves and leaf buds of three evergreen Asiatic species: R. aureum, R. brachycarpum, R. purdomii, six cultivars from Catawbiense Hybridum group (R. catawbiense): Catharine van Tol, Nova Zembla, Album Novum, Boursoult, Old Port, Hachmann’s Charmant and one cultivar from Yakushimanum Hybridum group: Koichiro Wada were investigated. Two plants from each accession were analysed. DNA was isolated by Ziegenhagen and Scholz [1998] protocol, dedicated to the difficult species in terms of high quality DNA extraction. After isolation, DNA was amplified with 19 RAPD primers. We obtained 255 RAPD markers and worked out a dendrogram, which illustrated the level of genetic diversity among the tested plants. Our study confirmed a close relationship between rhododendrons from Catawbiense Hybridum group. Another group was constituted by R. aureum and R. purdomii. The third group consisted of R. brachycarpum and Koichiro Wada cv.
PL
Oceniono pokrewieństwa różaneczników otrzymanych z kolekcji w Tomaszkowicach na Pogórzu Wielickim. Badaniami objęto trzy zimozielone gatunki botaniczne pochodzące z kontynentu azjatyckiego: R. aureum, R. brachycarpum, R. purdomii, sześć odmian hodowlanych należących do grupy mieszańców R. catawbiense: Catharine van Tol, Nova Zembla, Album Novum, Boursoult, Old Port, Hachmann’s Charmant oraz jedną odmianę hodowlaną z grupy mieszańców R. yakushimanum: Koichiro Wada. Testowano po dwie rośliny z każdego obiektu. Do izolacji DNA zastosowano technikę opisaną przez Ziegenhagen i Scholz [1998], przeznaczoną dla gatunków, dla których szczególnie trudno jest uzyskać DNA zadowalającej jakości. Różaneczniki należą bowiem do gatunków trudnych pod względem izolacji DNA. Następnie prowadzono reakcje PCR. Przebadano 19 starterów RAPD, które pozwoliły na otrzymanie 255 markerów. Na podstawie uzyskanych wyników opracowano dendrogram, który odzwierciedlał stopień pokrewieństwa genetycznego testowanych roślin. Badania potwierdziły występowanie bliskich powiązań genetycznych między różanecznikami z grupy mieszańców katawbijskich. Dwie osobne grupy tworzyły R. aureum i R. purdomii oraz R. brachycarpum i odm. Koichiro Wada.
EN
Crosses between Betula nana and B. utilis 'Doorenbos' were undertaken in order to obtain interspecific hybrids which could be characterized by wide spreading stems, strong branching habit, decorative clear white bark and an interesting shape of purple leaves. The research purpose was to examine genetic diversity of the 16 F1 and F2 putative progenies by using the RAPD-PCR method and the ploidy analysis. A total of 242 RAPD markers were scored with 24 primers and 220 (90.9%) polymorphic bands were found. In the NJ dendrogram, cluster I consisted of the female parent - B. nana and 12 hybrids and cluster II grouped the male parent - B. utilis 'Doorenbos' with 4 hybrids (F2/2, F1/8, F1/7 and F2/1). The 2-D scaling by PCoA was in agreement with the similarity index, i.e. two hybrids (F1/8, F2/2) grouped with the male parent while others with female parent. Classification of the hybrid plants by chromosome counting demonstrated that 13 hybrids were confirmed with accurate chromosome counts as being diploid (2n=2x=28) and 3 plants (F1/7, F1/8, F2/2) as triploid with 42 chromosomes.
EN
The presented system describes a concept for generating slowly varying signals to stimulate biological objects with magnetic field. The system enables independent control of up to 10 induction coil and it generates changeable signal inducing magnetic fields in the range from 0.1 to 150μT and the frequencies from 0.1 to 100 Hz. The proposed system was applied to investigate the influence of magnetic fields with the aforementioned parameters on physical and chemical properties in selected types of fruits.
EN
The paper presents findings showing the influence of amending soil with biochar and biomass ash on the germination rate of Virginia mallow. Comparative analyses examined the relationship between the applied dosage of the natural fertilizers and the effectiveness of seed germination as well as mass of the plants at the initial stage of growth. The obtained plant material was also examined for the contents of selected macro-elements (phosphorus, potassium and calcium). The study shows that biochar applied as a fertilizer, at specific dosage, may enhance germination rate of Virginia mallow seeds. Addition of biomass ash into the soil, at certain doses, significantly impacts the growth of mass in Virginia fanpetals at the initial stage of development. It has been determined that addition of biochar, or biomass ash or their mixtures into the soil alters the concentration of phosphorus, potassium and calcium in above-ground parts of the plants.
EN
This paper presents an influence of three different magnetic fields stimulation systems for growing strawberry fruits: permanent magnetic field generated by neodymium magnets, ”high” strengths slow varying amplitudes magnetic fields generated by coreless solenoids supplied by autotransformer and low-frequency and low-amplitude sinusoidal magnetic fields generator created for this research. The system is controlled by computer and allows for an independent control of up to 10 induction coils inducing magnetic fields with amplitudes from 0.1μT to 150μT and frequencies from 0.1Hz to 100Hz. The proposed set-up was used during the growing seasons for strawberry plants stimulation. An increase in the extract level was observed in the case of the alternating magnetic field stimulation with the parameters: frequency – from 50Hz to 100Hz and amplitude – from 50μT to 100μT.
20
Content available Mineral components analysis of selected dried herbs
38%
EN
This study includes the content of water, ash and volatiles in dried herbs like Oregano, Basil and de Provance. Moreover, amounts of H, N and C, and their calorific value were measured. The content of the following elements (Al, Ba, Ca, Cr, Cu, Fe, K, Mg, Mn, Na, Ni, P, S, Sr, Zn) in the tested herbs were established using the ICP-OES method. Basil leaves had the highest nitrogen and ash content at the level 4.5% and 15,9%. These herbs were characterized by a darker color as compared to oregano. Basil contained the richest source of Ca, K, Mg and P, while the lowest was found in herbs de Provence. The best source of Ca, K, Mg and P was basil, while their lowest amount was found in herbs de Provence (containing a mixture of different herbs), which mostly likely was the reason for the low amount of macro- and micro-components.
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