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Lesion of the amygdala nuclei in rats determines behaviors diagnostic for autism spectrum disorders

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Ptaszek K. , Plucinska K. , Myslinska D.

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nr Suppl.1

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Autism spectrum disorders (ASD) are a group of pervasive developmental disorder. All of them have been developing from birth, accompanying human through whole life and have strong influence on social functioning, communication as well as cognitive abilities. Neurobiological research (e.g. neuroimaging) indicate that patients with ASD demonstrate impairments of amygdala structure and functioning. It is worth to compare if previous results in human may be observed in animals. The aim of this study was display that lesion of amygdala nuclei may indicate behaviors diagnostic for ASD. For that purpose on a group of male Wistar rats (n=40) was conducted an electrolytic lesion of basolateral (BLA) or centromedial (CeA) amygdala. Animals were divided into groups separately for BLA and CeA: control (without operation), sham (operation, without lesion) and lesioned. After convalescence rats were observed in different behavioral tests which measured social functioning (social interactions), anxiety (elevated plus maze), spatial memory (water maze) and communication (smell preference). Obtain results suggest that amygdala lesion decreased social functioning or anxiety (BLA and CeA), communication (BLA), motor activity (CeA). In spite of this spatial memory increased (BLA). On the base of behavioral results it is likely that lesion of amygdala nuclei may be perceive animal model for further studies. (support: NN303 819040).

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Beneficial effect of high frequency stimulation of subthalamic nucleus on Vermicelli handling test behavior after partial nigral depletion in rats

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Grembecka B. , Dzbenska E. , Ziołkowska N. , Majkutewicz I. , Plucinska K. , Ptaszek K. , Wrona D.

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INTRODUCTION: Loss of dopaminergic neurons in substantia nigra pars compacta (SNpc) lead to motor deficits observed in patients with Parkinson’s disease (PD). The neurosurgical therapy of choice is high frequency stimulation of subthalamic nucleus (HFS-STN) improved motor control. The motor impairment depends on the progression of nigral degeneration and in rats model of PD may be measured by Vermicelli handling test (VHT). AIM(S): The purpose of this study was to evaluate the influence of HFS‑STN on VHT behavior in rats with early PD model, induced by 6-OHDA infusion into SNpc. METHOD(S): Male Wistar rats (n=12) were implanted unilaterally for HFS-STN and received a intranigral infusion of 6-OHDA. 5 days before infusion rats were trained on handle 7 cm lengths of vermicelli pasta and acclimated to video recording. Then, rats were subjected to HFS-STN for 7 days (1 h daily) at intensity just below triggering forelimb dyskinesia or SHAM stimulation. The VHT was providing in both groups each day. The number of adjustments made with each forepaw per each pasta piece, which allow definite Vermicelli asymmetry ratio (VAR) and time to eat were analyzed. PD model have been verified by the detection of tyrosine hydroxylase positive neurons in substantia nigra pars compacta. For a statistical analysis of the results, SPSS software was used. RESULTS: U-Man Whitney tests showed that HFS-STN stimulated rats consumed the pasta significantly faster than the SHAM (p≤0.001) across days 1st, 2nd, 5th, 6th, and 7th after 6-OHDA infusion. Interestingly, the VAR was higher in HFS-STN rats in 1st and 4th (p≤0.001 and p≤0.01) days in comparison to SHAM animals. The atypical behaviors were not observed. CONCLUSIONS: The HFS-STN applied in partial dopamine depleted rats influence on time of pasta eating and enhanced asymmetries in forepaw adjustments. The obtained results suggest that faster eating after HFS-STN may be related with amelioration of orofacial movements or increased motivation for food, but not with forepaw manipulation improvement. FINANCIAL SUPPORT: Supported by the Department of Animal and Human Physiology found 530-L124-D248-16.

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Subthalamic nucleus deep brain stimulation influence on erythrocyte number and hemoglobin levels in a rat model of early Parkinson's disease

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Grembecka B. , Koriat P. , Podlacha M. , Majkutewicz I. , Ptaszek K. , Plucinska K. , Wrona D.

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nr Suppl.1

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INTRODUCTION: Subthalamic nucleus deep brain stimulation (STN-DBS) is most effective treatment for Parkinson’s disease (PD) motor symptoms. A number of epidemiological studies have recently highlighted the association between hemoglobin (HGB) levels and PD risk. Interestingly, several lines of evidence confirm that STN-DBS increases regional cerebral blood flow and oxygen concentrations in target brain areas. AIM(S): Considering the close association between oxygen concentration, red blood number (RBC), and HBG, we hypothesized that enhanced blood flow during STN-DBS may influence peripheral RBC parameters in a rat model of early PD. METHOD(S): Male Wistar rats were implanted unilaterally for STN-DBS and received intranigral (substantia nigra pars compacta, SNpc) infusion of 6‑OHDA. After recovery, rats were subjected to STN-DBS for 7 days (1h daily, n=6) or SHAM stimulation (control, n=6). Immediately after collection, peripheral blood samples were analyzed using automated hematology analyzer (Cell Dyn 3700). The RBC number, hematocrit percentage (HCT), HGB concentration, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), and mean corpuscular hemoglobin concentration (MCHC) were measured. PD model was verified by the detection of tyrosine hydroxylase positive neurons in SNpc. For a statistical analysis of the results, SPSS 22.0 software was used. RESULTS: The Student’s t‑test showed that STN‑DBS rats had a significantly higher number of RBC in comparison to the SHAM rats (t(10)=‑2.912; p≤0.05). The HCT percentage slightly increased but differences did not reach statistical significance. Mann‑Whitney U tests showed that HGB level was higher in STN-DBS rats (Z=‑1.290; p≤0.05). CONCLUSIONS: The STN-DBS applied in a rat model of early PD has an influence on RBC number and HGB level. The obtained results suggest that there are peripheral compensation mechanisms for the increased oxygen demand during STN‑DBS in rats. FINANCIAL SUPPORT: Department of Animal and Human Physiology fund.

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Stimulation of the bed nucleus of the stria terminalis and the medial septal nucleus influences natural killer cell cytotoxicity of rats differing in responsiveness to novelty

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Myslinska D. , Plucinska K. , Glac W. , Grembecka B. , Listowska M. , Podlacha M. , Ptaszek K. , Wrona D.

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nr Suppl.1

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In our previous study we found that electrolytic lesion of the bed nucleus of the stria terminalis (BST) as well as the medial septal nucleus (MS) caused depression of the peripheral blood natural killer cell cytotoxicity (NKCC) in rats. In the present study we evaluated blood NKCC after 14 day electrical stimulation of the BST and the MS in conscious, freely behaving rats differing in responsiveness to novelty. Male Wistar rats divided into high (HR) and low (LR) responders to novelty, implanted with stimulating electrodes at the BST or at the MS, were subjected to 14 day electrical stimulation (constant current 0.1 ms duration cathodal pulses delivered at a frequency of 50 Hz during 30 min) of the BST and the MS. The chronic stimulation of the BST and the MS caused augmentation of blood NKCC in comparison to the sham operated group and to the baseline, which was more significant in HRs. A week after termination of the stimulation procedure NKCC returned to the baseline. The obtained results suggest that immunoenhancing effect on blood NK cell function is dependent on the behavioral outcome (intensive locomotor reaction) of the BST and the MS stimulation as well as on individual behavioral characteristics. This work was supported by a research grant NN303819040.