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nr 02
55-56
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nr 2
133-140
EN
The authors tried to determine the role of TH lymphocytes in the course of muscular trichinellosis in mice. The investigations were carried out on the CFW mice infected with 200 Trichinella spiralis larvae and treated with Cyclosporine A to suppresse TH1 lymphocytes. In animals treated with the drug more numerous cells inside the larval capsules and more numerous granulomas compared to control were seen. At the same time the mean number of larvae was lower (69,2%) than in control. The results show that TH1 lymphocytes do not play the significant role in the destruction of muscular larvae. This is the first conclusion and requires of course futher investigations.
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nr 4
661-666
EN
Experiments were carried out on male Balb/c mice, weighing 20 g. The Candida cells (Candida albicans L 45) were killed by heat-treatment and obsonized with mouse serum. Macrophages were obtained from the peritoneal cavity. Phagocytosis was executed by incubation 0,2 ml of suspension of macrophages with 0,2 ml of Candida suspension. Microscopic slides with cell smear were preparated. The phagocytic index and the percentage of phagocytizing macrophages were calculeted. It was found that infection with Trichinella spiralis or Trichinella pseudospiralis caused a significant reduction in the phagocytic capacity and the percentage of phagocytizing macrophages. This reduction depended on the intensity of invasion.
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nr 2
195-203
PL
Próbki mięsa zainfekowanego larwami włośnia poddawano działaniu ciśnienia hydrostatycznego 50 – 300 MPa przez 15 min. w temperaturze pokojowej. Badaniom mikroskopowym poddawano larwy w normalnym otoczeniu tkanki mięsnej, larwy wyizolowane z tkanki mięsnej metodą wytrawienia i preparaty histologiczne larw, barwione hematoksyliną i eozyną. Pełną dekontaminację mięsa uzyskano przy ciśnieniu 200 i 300 MPa.
EN
Samples of meat infected with Trichinella spiralis larvae were subjected to hydrostatic pressures of 50, 100, 150, 200 and 300 MPa for 15 min at room temperature. The pressurised samples, control samples, larvae isolated from the samples by enzymatic etching, and the histological specimens obtained from the infected meat samples were observed under microscope. The survival rate was determined as the percentage of larvae capable of moving in the course of microscopic observation of the larvae isolated from the meat samples. It has been found that pressures 200 MPa or higher are lethal to 100% of the larvae. At lower pressures, some of the larvae survive, but their bodies undergo some deformations (partial straightening or excessive twisting).
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