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EN
In the present work we investigated the ability of vitamin C to modulate clastogenic effects induced in cultured human lymphocytes by X-irradiation delivered at either high (1 Gy/min) or low dose rate (0.24 Gy/min). Biological effects of the irradiation were estimated by cytokinesis-block micronucleus assay including the analysis of the frequency of micronuclei (MN) and apoptotic cells as well as calculation of nuclear division index (NDI). The numbers of micronucleated binucleate lymphocytes (MNCBL) were 24.85 š 2.67% and 32.56 š 3.17% in cultures exposed to X-rays (2 Gy) delivered at low and high dose rates, respectively. Addition of vitamin C (1-20 mi g/ml) to the medium of cultures irradiated with the low dose rate reduced the frequency of micronucleated lymphocytes with multiple MN in a concentration-dependent manner. Lymphocytes exposed to the high dose rate radiation showed a U-shape response: low concentration of vitamin C significantly reduced the number of MN, whereas high concentration influenced the radiation-induced total number of micronucleated cells insignificantly, although it increased the number of cells with multiple MN. Addition of vitamin C significantly reduced the fraction of apoptotic cells, irrespective of the X-ray dose rate. These results indicate that radiation dose rate is an important exposure factor, not only in terms of biological cell response to irradiation, but also with respect to the modulating effects of antioxidants.
EN
The purpose of this paper was to determine the vitamin C content and the selected consumption features (tastiness and cooking type) in edible potato tubers with colored flesh. The research material consisted of tubers of eight edible potato cultivars collected from a field experiment conducted in 2021 and 2022 at the Agricultural Experimental Station in Zawady of Siedlce University of Natural Sciences and Humanities (52°03’N; 22°33’E). The following cultivars were grown: two cultivars with red flesh – Rote Emma and Herbie 26, five cultivars with purple flesh – Blaue Annelise, Provita, Salad Blue, Vitelotte and Bora Valley, as well as one traditional cultivar, light yellow fleshed – Eurostar. The cultivar features of the potato tubers significantly modified the content of vitamin C and they determined cooking properties of the potato. The cultivars with red and purple flesh accumulated more vitamin C than the traditional cultivar. The cultivar Rote Emma and Salad Blue had the best tastiness, while the cultivar Herbie 26 and Bora Valley proved to be the least tasty. The red and purple-fleshed cultivars were dominated by the salad cooking type A and they differed significantly from the traditional cultivar Eurostar. Potato tubers with colored flesh are a valuable and attractive offer to the consumer.
PL
Celem pracy było badanie wpływu metody mrożenia na szybkość degradacji witaminy C podczas zamrażalniczego przechowywania kapusty brukselskiej. Materiałem stosowanym w badaniu były dwie odmiany kapusty brukselskiej: Lancelot i Valiant. Po blanszowaniu kapustę brukselską mrożono trzema metodami: skroplonym dwutlenkiem węgla, konwencjonalnie i skroplonym azotem. Po mrożeniu kapustę przechowywano w temperaturze - 20š2°C przez 60 tygodni. Badania kinetyczne wykazały, że zarówno metoda mrożenia, jak i odmiana stosowanej w badaniu kapusty, nie wpływają na mechanizm degradacji witaminy C. Szybkość degradacji stosuje się do kinetyki reakcji pierwszego rzędu. Metoda mrożenia i odmiana wpływają istotnie na wartość szybkości reakcji degradacji. Najwolniej rozkład witaminy C przebiegał w partiach mrożonych skroplonym CO2, a najszybciej w mrożonych metodą konwencjonalną. Mrożenie CO2 spowalniało szybkość degradacji około 2 razy w porównaniu z mrożeniem konwencjonalnym oraz ok. 1,4 razy w partii mrożonej azotem. Lepszą zachowalnością witaminy C charakteryzowała się odmiana Valiant. Szybkość rozkładu w tej odmianie przebiegała około 1,3 razy wolniej w porównaniu z odmianą Lancelot.
EN
The effect of freezing method on the rate of vitamin C degradation during frozen storage of two varieties of Brussels sprouts (Lancelot, Valiant) was investigated. Blanched vegetables were frozen by three methods: with liquid carbon dioxide, conventional in air, and with liquid nitrogen. After freezing, the Brussels sprouts were kept at temperature of - 20 š 2°C for 60 weeks. Kinetic investigations showed that neither the freezing method nor the vegetable variety used affected the mechanism of vitamin C degradation. The rate of degradation is applied to the first order reaction kinetics. The freezing method and variety have a significant influence on the degradation reaction rate. Vitamin C degradation rate was the slowest in the vegetables frozen with liquid CO2, while the fastest in those frozen by the conventional method. Freezing in CO2 reduced the degradation rate about twice as compared to the conventional freezing and by about 1.4 time in the vegetables frozen in liquid nitrogen. Vitamin C was better preserved in the Valiant variety. The rate of degradation in this variety was about 1.3 time slower than in the Lancelot variety.
EN
The objective of the research was to determine the effect of herbicides and herbicides combined with biostimulants on the leaf greenness index SPAD and selected potato characteristics, i.e. plant height, yield of large tubers and the vitamin C content. A three-year experiment was conducted to examine the following factors: factor I – three potato cultivars: Bartek, Gawin, Honorata; factor II – five methods of an application of herbicides and biostimulants: 1. control unit without chemical control, 2. the Harrier 295 ZC herbicide (linuron + clomazone), 3. the Harrier 295 ZC herbicide (linuron + clomazone) and the Kelpak SL biostimulant (Ecklonia maxima), 4. the Sencor 70 WG herbicide (metribuzin), 5. the Sencor 70 WG herbicide (metribuzin) and the Asahi SL biostimulant (sodium para-nitrophenol, sodium ortho-nitrophenol, sodium 5-nitroguaiacol). The cultivars investigated in the experiment significantly affected the leaf greenness index SPAD, plant height, yield of large tubers and the vitamin C content. The herbicides and biostimulants enhanced the values of the examined potato characteristics. Linear correlation coefficients confirm a strong, significant, positive correlation between the SPAD index and plant height, yield of large tubers and vitamin C content.
PL
Witamina C uważana jest za jedną z najważniejszych substancji w organizmie. Związek ten uczestniczy w wielu procesach metabolicznych, jednakże nie jest produkowany samodzielnie przez organizm ludzki. Przegląd dostarcza informacji o właściwościach i znaczeniu witaminy C. Główną uwagę zwrócono na zastosowanie witaminy C w leczeniu wybranych chorób zakaźnych, terapiach przeciwnowotworowych, jak również aplikacjach kosmetycznych. Ponadto omówiono najważniejsze funkcje i właściwości witaminy C. Omówiono także możliwość transportu witaminy C w systemach dostarczania leków, m.in. z wykorzystaniem liposomów.
EN
Vitamin C is considered one of the most important substances in the organism. This compound is involved in many metabolic processes; however, it is not produced independently by the human organism. This review provides information on the properties and role of vitamin C. The main attention has been paid to the use of vitamin C in the treatment of selected infectious diseases, anticancer therapies as well as cosmetic applications. In addition, the most important functions and properties of vitamin C are discussed. The possibility of transporting vitamin C in drug delivery systems using liposomes was also discussed.
PL
Badano: brokuł włoski (Brassica oleracea L. var. botrytis italica, odmiana Monaco), kapusta głowiasta biała (Brassica oleracea L.var.capitata, odmiana Krautman), kapusta włoska (Brassica oleracea L. var. sabauda, odmiana Wirosa). Warzywa przechowywano w kontrolowanej atmosferze (CA) oraz dla porównania wyników w powietrzu (K). Brokuły i kapustę włoską przechowywano w CA o składzie: 4% CO2, 3% O2, 92% N2, w temperaturze 0-l°C, kapustę głowiastą białą w CA o składzie: 5% CO2, 3% O2, 93 % N2, w temperaturze 2-3°C. Analizowano intensywność oddychania, zawartość witaminy C, barwników chlorofilowych i cechy sensoryczne. Wykazano, że kontrolowana atmosfera zmniejsza intensywność oddychania badanych warzyw średnio o 20 - 40% oraz około dwukrotnie szybkość degradacji witaminy C w stosunku do warzyw przechowywanych w powietrzu. Szybkość degradacji witaminy C w warzywach przechowywanych w CA stosuje się do kinetyki I rzędu, natomiast przechowywanych w powietrzu do kinetyki "0"-rzędu. Kontrolowana atmosfera ogranicza przemianę barwników chlorofilowych i sprzyja zachowaniu intensywnej zielonej barwy.
EN
Broccoli (Brassica oleracea L. var. botrytis italica, variety Monaco), headed cabbage (Brassica oleracea L. var. capitata, variety Krautman), and savoy (Brassica oleracea L. var. sabauda, variety Virosa) were tested. The vegetables were stored in a controlled atmosphere (CA) and - for comparison - in the air (K). Broccoli and savoy were stored in CA of the composition: 4% CO2, 3% O2, 92% N2, at the temperature 0-l°C, while headed cabbage in CA consisting of 5% CO2, 3% O2, 93% N2, at the temperature 2-3°C. The respiration intensity, content of vitamin C and chlorophyll pigments and sensorial features were analysed. It was shown that the controlled atmosphere caused a 20 to 40% reduction of the respiration intensity of tested vegetables on average and about double reduction of vitamin C degradation rate in relation to vegetables stored in the air. The vitamin C degradation rate in the vegetables stored in CA applies to the first order kinetics, while of those stored in the air to the zero order. The controlled atmosphere reduces the transformation of chlorophyll pigments and contributes to the preservation of an intensive green colour.
EN
Purpose: To evaluate the hypothesis that vitamin C improves the efficacy of Helicobacter pylori eradication in smokers when combined with pantoprazole, amoxicillin, and clarithromycin. Materials and methods: The study completed 90 subjects of 98 enrolled, 58 smokers 32 nonsmokers. Helicobacter pylori status was determined by two methods, CLO test, and histology. Vitamin C (500 mg) was administered three times daily. The patients were considered as cured of H. pylori if the CLO test result and histology were negative 4 weeks after completion of eradication therapy. Results: Smokers had lower effectiveness of eradication therapy than non-smokers and the administration of vitamin C had not affected the outcome of eradication therapy. Conclusions: Vitamin C (500 mg tid) does not improve the eradication therapy when pantoprazole, amoxicillin, and clarithromycin were used.
PL
Badano zawartość witaminy C i stopień zmiatania wolnych rodników DPPH w mrożonej kapuście brukselskiej przechowywanej w -20, -30 i -50°C. Na podstawie otrzymanych wyników stwierdzono, że w temperaturze -50°C zmiany degradacji witaminy C oraz właściwości antyoksydacyjnych przebiegały najwolniej. Ponadto stwierdzono, że mniejszą stabilnością charakteryzowały się właściwości antyoksydacyjne niż witamina C.
EN
Vitamin C content and activity in quenching of DPPH free radicals were assayed at intervals in samples of frozen Brussels sprouts kept at -20, -30 and -50°C. It was found that changes in these two characteristics were the slowest at -50°C. Besides, a drop in vitamin C level was lesser than that in the antioxidant capacity.
9
100%
Eliksir
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2023
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tom nr 12
15--20
11
Content available remote Effects of organic compounds on the macroalgae culture ofAegagropila linnaei
88%
EN
The effects of the impact of four organic compounds (ascorbic acid, biotin, glucose and sucrose) on ash, protein, fiber, fat and amino acid contents in the freshwater Aegagropila linnaei biomass were examined in 7 and 14 days of cultivations in high concentrations of tested compounds (100 mg L-1). The presence of examined organic compounds had a negligible effect on the development of algae and their biomass composition. There were no significant differences in the amino acids composition in the biomass in the presence of organic compounds compared to the test system. However, the increase in ash content was observed irrespective of the cultivation time in the case of all used organic compounds. Only slight differences in crude fat concentration were observed in the case of 7 days cultivation with ascorbic acid, biotin and sucrose, while the highest increase of ash content was observed after 14 days of supplementation with glucose. None of the compounds affected changes in amino acid content in the Aegagropila linnaei biomass. The results suggest that an environment enriched with the test organic compounds had only minimal, or at most short-term, effects on the algal biomass composition.
EN
The work was an attempt to determine and describe certain quality characteristics of edible potato on sale in Siedlce and Międzyrzec Podlaski. Potato tubers for examination were purchased in 3 kinds of retail outlets (ten shops of each kind) in east-central Poland. A total of 90 samples were collected to determine tuber size, protein and vitamin C content, reducing sugars and sucrose content. Potatoes purchased in all kinds of outlets met the standards set for tuber size. Potatoes bought in supermarkets had a better chemical composition than tubers purchased in groceries or fruit and vegetable shops. All the potato tubers were a good staple.
EN
Purpose: Carbofuran toxicity on rats was studied during sub-acute exposure. This work was undertaken to evaluate the protective effect of aqueous black tea extract and vitamin C against a rat model of oxidative stress induced by treatment with carbofuran, an organocarbamate insecticide. Materials and methods: The levels of lipid peroxidation, reduced glutathione and ascorbic acid were assessed by determining the extent of oxidative stress in the erythrocytes of rats. Results: The results clearly demonstrated that the treatment of rats with sub-acute concentration of carbofuran caused significant elevation in the levels of oxidative stress and decrease in the contents of glutathione and ascorbic acid. The introduction of black tea extract and vitamin C augmented the antioxidant defense mechanism in alleviating the carbofuran induced oxidative stress. Conclusion: The findings that the pretreatment with black tea and vitamin C can mitigate carbofuran induced toxicity lend evidence that supplementation with either black tea extract and/or vitamin C have a therapeutic potential in amelioration of oxidative stress in mammalian systems
EN
The aim of this study was to estimate the content of exogenous antioxidants in kiwi fruits available in Gdynia, Poland, and their ability to reduce free radicals. The test material consisted of mini kiwi fruit (Actinidia arguta) of the ‘Hortgem Thai’ cultivar and kiwi fruit (Actinidia deliciosa) of the ‘Hayward’ cultivar. The carotenoid pigments were determined by the Lichtenthaler method, vitamin C in accordance with PN-A-04019:1998, polyphenols by the Folin-Ciocialteu method and the ability to reduce free radicals using a synthetic DPPH radical. One-way analysis of variance was used to determine the effect of fruit species on the quality traits analysed. The significance of differences between the means was determined using Tukey’s test. Relationships were considered statistically significant at p < 0.05. Correlation coefficients between the antioxidant activity of kiwi fruit and the content of exogenous antioxidants were also determined. The results showed that Actinidia arguta has significantly higher contents of exogenous antioxidants than Actinidia deliciosa. The antioxidant properties of this fruit were also found to be mainly due to its vitamin C content.
EN
The important role of fruits in human health and nutrition has been better understood with the recent studies on biochemical contents of fruits having antioxidant properties. Being one of the similar studies, in this study, total antioxidant capacity (TAC), phenolic compound, organic acid, and vitamin C contents of three plum species (Prunus domestica L., Prunus cerasifera Ehrh., and Prunus spinosa L.) grown in Van locality (Turkey) were identified, and the correlation between the measured values was investigated. Phenolic compound, organic acid, and vitamin C contents were determined by high-performance liquid chromatography (HPLC) method. Analysis of phenolic compound indicated that chlorogenic acid was the predominant phenolic compound, and the highest value was measured in P. spinosa L. as 12.985 mg kg−1. Malic acid was the predominant organic acids and the highest value was measured in P. spinosa L. as 1.245 g 100 g−1. The highest TAC and vitamin C contents were also measured in P. spinosa L. as 1.021 mmol TE kg−1 and 25.492 mg 100 g−1, respectively. P. spinosa L. was found to be superior to the other two species with respect to antioxidant capacity and other biochemical contents. A significant (P ≤ 0.01) and positive correlation was reported between antioxidant capacity and vitamin C content.
EN
The aim of the study was to determine the influence of drip irrigation on the nutritive value of winter squash ‘Rouge vif d’Etampes’ fruits analyzed directly after the harvest and after the storage. The plants were grown on the very light soil in the region of decreased rainfall amounts during the vegetation period. The field experiment was conducted in 2007-2008 at Kruszyn Krajeński near Bydgoszcz on the soil of quality class V-VI (very weak and weak-rye-soil-complex). The field water capacity in the soil layer 0-50 cm was 57.5 mm, while the effective useful retention amounted 29.3 mm. The experiment was designed as one-factorial trial in four replications; drip irrigation as the experiment factor was applied. As the control the plots without irrigation were considered. The single plot area was 11.2 m2. The drip irrigation was conducted according to the soil tensiometer indications (-0.04 MPa). The research material was the eatable parts of fresh fruits that were analyzed directly after the harvest and after the storage for six months at the temperature 10°C and the relative air humidity 75%. The drip irrigation performed during the cultivation of winter squash increased the content of vitamin C, total carotenoids including ß-carotene, as well sugars in the fruits, both after the harvest and after the storage. After six months of the storage the rise of the content of dry matter, total carotenoids including ß-carotene, as well monosaccharides was noted, while the level of vitamin C, total sugar, and saccharose have been reduced.
EN
Studies were conducted in the years 2002-2004 at Experimental Station of Faculty of Horticulture in Garlica Murowana near Kraków. The experiment was set up in Novcmber 2000. Six strawberry cultivars were investigated. Strawberry plants started yielding in year 2002. In the first year of cropping 'Elkat', 'Kent' yielded at the highest level. In year 2003 every cultivars gave the smallest yield. In the third year of cropping 'Kent' and 'Selva' cultivars showed the highest yields. For 'Elkat' and 'Senga Sengana' the highest unmarketable fruit crop was recorded. The differences in one fruit mass from the first and late crops were the biggest for 'Elkat', 'Gerida' and 'Kent' in years 2002 and 2003. For 'Gerida', 'Elsanta' and 'Kent' the highest percentage of fruit of diameter 25 mm was recorded.
PL
Badania prowadzono w latach 2002-2004 w Stacji Doświadczalnej Wydziału Ogrodniczego w Garlicy Murowanej koło Krakowa. Doświadczenie zostało założone w listopadzie 2000 r. Badaniami objęto sześć odmian truskawek: 'Elkat', 'Elsanta', 'Gerida', 'Kent', 'Selva' oraz 'Senga Sengana'. Rośliny rozpoczęły owocowanie w 2002 r. W pierwszym roku plonowania uzyskano najwyższe plony owoców truskawek odmiany 'Elkat' oraz 'Kent'. W roku 2003 wszystkie odmiany cechowały się najmniejszym plonem w porównaniu z uzyskanymi z pozostałych dwóch lat badań. W trzecim roku owocowania największy plon owoców dały odmiany 'Kent' oraz 'Selva'. Dla odmian 'Elkat' oraz 'Senga Sengana' odnotowano najwyższy plon owoców niehandlowych zarówno w poszczególnych latach, jak i łącznie po trzech sezonach. Największe różnice w masie jednego owocu z pierwszych i ostatnich zbiorów stwierdzono w przypadku odmian 'Elkat', 'Gerida' oraz 'Kent' w latach 2002 i 2003. W przypadku odmian 'Gerida', 'Elsanta' oraz 'Kent' odnotowano największy procentowy owoców o średnicy 25 mm.
EN
Plant nutrition is one of the significant factors determining the quantity and quality of crop production. Therefore, the objective of the experiment was to express the dependence numerically (statistically) of the selected yield parameters of radish on the contents of ammonium and nitrate nitrogen occurring in the soil growing medium in different periods of radish vegetation. The experiment was performed in the plastic greenhouse with aeration and irrigation manually operated. It had three variants and was established by the method of random plot arrangement with a threefold repetition of variants. The variants differed in the quantity of vermicompost in the soil growing medium (0%, 10%, 20%). The achieved results indicate that the yield parameters of radish were more dependent on the contents of N–NO3- than on the contents of N–NH4+ occurring in the soil substrate. The quantity of N–NO3-, occurring in the soil growing medium in the initial period of the growing season, had impact on the quantity of yield. The quantity of N–NO3-, present in the soil substrate in the second half of the growing season, had impact mainly on the content of vitamin C and content of nitrates in roots. As the N–NO3- content of the soil growing medium increased, the content of nitrates was increased and content of vitamin C was decreased. The dependence between the contents of N–NH4+ in the substrate and root yield was insignificant. The quantity of radish root yield depended predominantly on the contents of Nin occurring in the soil growing medium at the beginning of the growing season. On the contrary, the content of nitrates and vitamin C (influencing the yield quality) was dependent mostly on the contents of Nin present in the soil substrate in the second half of radish growing season. Along with the increase in the content of Nin, the root yield and the content of nitrates in roots also increased, while the content of vitamin C was decreased.
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