Wyniki wyszukiwania - Biblioteka Nauki

1

Rapid and simple screening of transgenic mice: novel extraction-free, filter-based PCR genotyping from blood samples

100%

Suematsu N. , Isohashi F.

Acta Biochimica Polonica

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2006

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tom 53

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nr 3

613-613

EN

We evaluated the effectiveness of using Flinders Technology Associates (FTA) filter paper for the polymerase chain reaction (PCR) genotyping of transgenic mice. Tail prick blood sample dried on an FTA filter disc was processed for genomic PCR. It is easy and rapid to prepare DNA templates because the protocol is extraction-free and only requires minimal handling of wash briefly bloodstained FTA filter discs. Progeny of a transgene-positive founder mated with wild-type mice was screened for the presence of the transgene by the filter-based PCR using transgene-specific primers. The resulting amplicons with expected sizes of 3134 bp, 1152 bp, 877 bp and 688 bp were robust and reproducible, allowing a distinction between transgenic (n=44) and wild-type (n=47) mice showing no signal. The filter-based PCR screening took only half a day. The present study confirmed the validity and usefulness of the novel rapid extraction-free genotyping method.

2

Differences in glutathione S-transferase pi expression in transgenic mice with symptoms of neurodegeneration

86%

Kaźmierczak B. , Kuźma-Kozakiewicz M. , Usarek E. , Barańczyk-Kuźma A.

Acta Biochimica Polonica

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2011

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tom 58

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nr 4

621-626

EN

Glutathione S-transferase pi (GST pi) is an enzyme involved in cell protection against toxic electrophiles and products of oxidative stress. GST pi expression was studied in transgenic mice hybrids (B6-C3H) with symptoms of neurodegeneration harboring SOD1G93A (SOD1/+), Dync1h1 (Cra1/+) and double (Cra1/SOD1) mutations, at presymptomatic and symptomatic stages (age 70, 140, 365 days) using RT-PCR and Western blotting. The main changes in GST pi expression were observed in mice with the SODG93A mutation. In SOD1/+ and Cra1/SOD1 transgenics, with the exception of cerebellum, the changes in GST pi-mRNA accompanied those in GST pi protein. In brain cortex of both groups the expression was unchanged at the presymptomatic (age 70 days) but was lower at the symptomatic stage (age 140 days) and at both stages in hippocampus and spinal cord of SOD1/+ but not of Cra1/SOD1 mice compared to age-matched wild-type controls. In cerebellum of the presymptomatic and the symptomatic SOD1/+ mice and presymptomatic Cra1/SOD1 mice, the GST pi-mRNA was drastically elevated but the protein level remained unchanged. In Cra1/+ transgenics there were no changes in GST pi expression in any CNS region both on the mRNA and on the protein level. It can be concluded that the SOD1G93A but not the Dync1h1 mutation significantly decreases detoxification efficiency of GST pi in CNS, however the Dync1h1 mutation reduces the effects caused by the SOD1G93A mutation. Despite similarities in neurological symptoms, the differences in GST pi expression between SOD1/+ and Cra1/+ transgenics indicate a distinct pathogenic entity of these two conditions.

3

Effects of growth hormone on neuroendocrine function

86%

Bartke A. , Chandrashekar V. , Steger R. W.

Acta Neurobiologiae Experimentalis

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1996

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tom 56

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nr 3

4

Functional analysis of spermatocyte-specific hst70 gene promoter in transgenic mice

86%

Widlak W. , Markkula M. , Krawczyk Z. , Huhtaniemi I.

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nr 2

5

CD45 in memory and disease

72%

Tchilian E. Z. , Beverley P. C. L.

Archivum Immunologiae et Therapiae Experimentalis

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2002

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tom 50

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nr 2

6

Alpha-synuclein A30P point-mutation generates age-dependent nigrostriatal deficiency in mice

72%

Plaas M. , Karis A. , Innos J. , Rebane E. , Baekelandt V. , Vaarmann A. , Luuk H. , Vasar E. , Koks S.

Journal of Physiology and Pharmacology

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2008

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tom 59

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nr 2

EN

Lewy bodies are mainly composed of alpha-synuclein (SNCA) and specific mutations in SNCA gene are related to familial forms of Parkinson's disease (PD). The purpose of our study was to generate a mouse line with A30P knock-in point mutation in SNCA gene and to test if a single point-mutation is able to turn otherwise normal SNCA into a toxic form. The behavioral profile of SNCA A30P mice was followed for 16 months. Generally, these mice are healthy and viable without any obvious abnormalities. Starting from the age of 13 months mice developed a significant deficit in motor performance tests related to nigrostriatal function (ink-test and beam walk). In other tests (motility boxes, rotarod) mice continuously performed normally. Moreover, SNCA A30P mice expressed the altered sensitivity to VMAT2 inhibitor reserpine, possibly reflecting a functional deficiency of dopamine. Indeed, mice at 15 months of age had significantly reduced levels of dopamine and its major metabolite DOPAC in the striatum, and reduced levels of dopamine in the mesolimbic system. The present study confirms that SNCA plays an important role in the development of PD and an insertion of a single point mutation is sufficient to generate age-related decline in specific motor performance. The generated mouse line has a potential to become a model for PD with comparable time course and phenotype.

7

Abnormal purine and pyrimidine nucleotide content in primary astroglia cultures from HGPRT - deficient transgenic mice

72%

Pelled D. , Sperling O. , Zoref-Shani E.

Cellular and Molecular Biology Letters

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1999

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tom 04

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nr 3

8

Unexpected reaction of anti-H-2d serum with thymic lymphoma cells derived from transgenic B6-H-2b TCR anti-HY-Db mice

72%

Matuszyk J. , Ziolo E. , Kobzdej M. , Strzadala L.

Archivum Immunologiae et Therapiae Experimentalis

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1996

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tom 44

|

nr 2-3

9

Histological changes of testes in growth hormone transgenic mice with high plasma level of GH and insulin-like growth factor-1

58%

Piotrowska K. , Sluczanowska-Glabowska S. , Kucia M. , Bartke A. , Laszczynska M. , Ratajczak M. Z.

Folia Histochemica et Cytobiologica

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2015

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tom 53

|

nr 3

10

Human antibody expression in transgenic mice

58%

Bruggemann M.

Archivum Immunologiae et Therapiae Experimentalis

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2001

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tom 49

|

nr 3

203-208

11

Comparison of acetaminophen toxicity in primary hepatocytes isolated from transgenic mice with different apolipoprotein E alleles

58%

Mezera V. , Kucera O. , Moravcova A. , Peterova E. , Rousar T. , Rychtrmoc D. , Sobotka O. , Cervinkova Z.

Journal of Physiology and Pharmacology

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2015

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tom 66

|

nr 6

12

Autoimmunity caused by T cells escaping negative selection

58%

Rolink A.

Archivum Immunologiae et Therapiae Experimentalis

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2011

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tom 59

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nr 5

13

Loss of T cell receptor diminished tumorigenicity of thymocyte-derived lymphoma cells in the T cell receptor transgenic mice

58%

Kobzdej M. , Matuszyk J. , Ziolo E. , Strzadala L.

Archivum Immunologiae et Therapiae Experimentalis

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1997

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tom 45

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nr 4

14

DNA constructs designed to produce short hairpin, interfering RNAs in transgenic mice sometimes show early lethality and an interferon response

58%

Cao W. , Hunter R. , Strnatka D. , McQueen C. A. , Erickson R. P.

Journal of Applied Genetics

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2005

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tom 46

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nr 2

EN

Arylamine N-acetyltransferase (NAT) genes were targeted for inhibition using short hairpin RNA (shRNA) using two different RNA polymerase III promoters. Constructs were developed for NAT1 and NAT2, the endogenous mouse genes, and for human NAT1. There were fetal and neonatal deaths with these constructs, perhaps due in part to an interferon response as reflected in increases in oligoadenylate synthetase I mRNA levels. Seven out of 8 founders with the U6 promoter generated offspring but only 2 gave positive offspring. Out of 15 founders for H1 promoted constructs, only 4 had positive offspring. When transgenic lines were successfully established, the expression of the targeted genes was variable between animals and was not generally inhibitory.

15

From embryonic stem cells and induced pluripotent cells to lymphocytes

51%

Seiler K. , Tornack J. , Karjalainen K. , Tsuneto M. , Melchers F.

Archivum Immunologiae et Therapiae Experimentalis

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2011

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tom 59

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nr 5