Wyniki wyszukiwania - Biblioteka Nauki

1

Exploiting iron-binding proteins for drug delivery

100%

Bialasek M. , Kubiak M. , Gorczak M. , Braniewska A. , Kucharzewska-Siembieda P. , Krol M. , Taciak B.

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nr 5

2

Effects of transferrin on aromatase activity in porcine granulosa cells in vitro

100%

Durlej M. , Duda M. , Knapczyk K. , Slomczynska M.

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2008

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tom 46

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nr 4

423-428

3

Transferrin genotypes as genetic markers of lifetime prolificacy of ewes in a flock of prolific sheep 09

100%

Steppa R. , Slosarz P. , Strojna A. , Stanisz M.

Annales Universitatis Mariae Curie-Skłodowska. Sectio EE: Zootechnica

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2007

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tom 25

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nr 1

4

Doxorubicin-transferrin conjugate selectively overcomes multidrug resistance in leukaemia cells

100%

Lubgan D. , Jozwiak Z. , Grabenbauer G. G. , Distel L. V. R.

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2009

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tom 14

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nr 1

113-127

EN

Neoplastic cells frequently have an increased number of transferrin receptors. Coupling transferrin to an anti-neoplastic drug has the potential to overcome multidrug resistance (MDR). The purpose of this study was to examine the distribution and action of doxorubicin-transferrin conjugate (DOXTRF) in a leukaemia cell line (HL60), a multidrug-resistant leukaemia cell line (HL60ADR) and a normal tissue cell line (human fibroblasts). The intracellular accumulation of DOX and DOX-TRF was monitored by direct fluorescence. More DOX-TRF than free DOX was delivered to the tumour cells, and consecutively the levels of DNA double-strand breaks and apoptosis increased even in the multidrug-resistant cell line. In the normal tissue cell line, DOX-TRF did not accumulate, and therefore, the levels of DNA double-strand breaks and apoptosis did not increase. Cell viability was determined using the MTT assay. The IC50 for DOX-TRF was lower than the IC50 value for the free drug in both leukaemia cell lines. The IC50 values for the HL60 cells were 0.08 μM for DOX and 0.02 μM for DOX-TRF. The IC50 values for HL60ADR cells were 7 μM for DOX and 0.035 μM for DOX-TRF. In conclusion, DOX-TRF was able to overcome MDR in the leukaemia cell lines while having only a very limited effect on normal tissue cells.

5

Individual genetic variability within synthetic breeding lines of carp against the background of the electrophoretic fractionation of blood plasma transferrin

100%

Starmach J.

Acta Hydrobiologica

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1987

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tom 29

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nr 1

6

Glycoforms of six serum glycoproteins in a patient with congenital disorder of glycosylation type I

100%

Ferens-Sieczkowska M. , Zwierz K. , Midro A. , Katnik-Prastowska I.

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tom 50

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nr 1

7

The use of antibodies labelled with dyes for fast and simple assay of some human proteins by immunofiltration technique

100%

Szewczuk A. , Kuropatwa M. , Rapak A.

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1992

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tom 40

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nr 5-6

8

Inter and intra subpopulation genetic variability of roe deer (Capreolus capreolus L.) assessed by I and II class genetic markers

80%

Kamieniarz R. , Wolc A. , Lisowski M. , Dabert M. , Grajewski B. , Steppa R. , Szwaczkowski T.

Folia Biologica

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2011

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tom 59

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nr 3-4

9

An overall view of the process of the regulation of human iron metabolism

80%

Formanowicz D. , Formanowicz P.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2011

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tom 92

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nr 2

10

Polimorfizm transferyny a plenność u owiec

80%

Bojczuk H. , Bojczuk B.

Zeszyty Problemowe Postępów Nauk Rolniczych

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1988

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tom 352

RU

Зависимость между полиморфизмом трансферрина и гемоглобина с одной и оплодотворимостыо с другой стороны исследовали в двух стадах польской низинной овцы. В стадах не установлено стетистически существенных различий в частоте рождаемости близнецов в результате спаривания овец с разными генотипами гемоглобине и происходящих от баранов с различными гемотипами.

EN

The dependence between the polymorphism of transferrin and hemoglobin on the one hand and fecundity on the other was investigated in two flocks of Polish lowLand sheep. No statistically significant differences in the frequency of birth Df twins after ewes and rams of different transferrin and hemoglobin genotypes aere found in any of these flocks. No significant differences were found, either, In the freuency of twin births from those of different hemoglobin genotypes and Driginating from rams of different hemotypes.

11

Serum ferritin, iron and transferrin in women with thyrotoxic Graves' disease before and after methimazole treatment

80%

Zwirska-Korczala K. , Buntner B. , Sobieraj H. , Ostrowska Z. , Kniazewski B. , Swietochowska E.

Acta Physiologica Polonica

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1990

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tom 41

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nr 7

EN

Żwirska-Korczala K., Buntner В., Sobieraj H., Ostrowska Z., Kniażewski B. and Świętochowska E.: Serum ferritin, iron and transferrin in women with thyrotoxic Graves’ disease before and after methimazole treatment. Acta physiol, pol., 1990, 41 (2): 166-166. We investigated iron metabolism in 47 women with thyrotoxic Graves’ disease. Serum iron, ferritin, transferin, triiodothyronine and thyroxine concentrations were RIA measured before and after methimazole treatment when patients became euthyroid. The control group consisted of 52 healthy women. We noted that serum ferritin levels and the ferritin to transferrin ration were significantly lower while the iron to ferritin ratio was higher in patients before and after methimazole therapy. Iron concentration as well as the iron to transferrin and the iron to thyroid hormone ratios were decreased only before treatment.

12

Wykorzystanie badan grup krwi i bialek polimorficznych u owiec w praktyce hodowlanej

80%

Rychlik T. , Janik A. , Duniec M.

Biuletyn Informacyjny. Instytut Zootechniki

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1996

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tom 34

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nr 4

51-60

13

Effect of suramin on human interferon alpha binding to cell receptors

80%

Kandefer-Szerszen M. , Ruuth K. , Lundgren E.

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nr 1

14

Genetic characteristics of Mouton Charollais sheep bred in Poland and their crossbreds with Polish Merino

80%

Rychlik T. , Duniec M. J.

Roczniki Naukowe Zootechniki

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1999

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tom 26

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nr 4

15

Genetic variation in a fluctuating population of the yellow-necked mouse Apodemus flavicollis

80%

Wojcik A. M.

Acta Theriologica

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1993

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tom 38

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nr 3

EN

Protein polymorphism in 767 yellow-necked mice Apodemus flavicollis fMelchior, 1834), was studied in spring and autumn during five years in the Białowieża National Park, Eastern Poland. Two peaks and two declines of population number were observed, and population density varied from 3 to 63 inds/ha. Twenty three loci of proteins were studied, 4 of them were polymorphic; and mean heterozygosity of the studied population was H = 0.057, The greatest variability was observed in the transferrin locus and 4 alleles (7>/a, Trfb, Trfc, and Trfd) were described. The frequency of the Trf" allele and Trf /Trf" homozygotes was negatively correlated with population density in autumn, whereas the frequency of the Trfc allele and TrfaITrfc heterozygotes was positively correlated with population density in autumn. The frequency of heterozygotes was higher than the frequency of homozygotes during each spring. Significant excess of Trf"/Trfc heterozygotes, compared to the values expected from the Hardy-Weinberg ratio, was observed in autumn 1983 and spring 1984, 1986, and 1987. Natural selection may be an important force in maintenance of the transferrin polymorphism in A. flavicollis. It is suggested that heterozygotes survive better than homozygotes during winter and at high population densities. It is also supposed that non-selective forces influence on the Trf allele frequency changes in the studied population of A. flavicollis.

16

Association between transferrin polymorphism and some biochemical characters of blood in lambs of Polish long-wool sheep

80%

Kmiec M.

Genetica Polonica

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1992

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tom 33

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nr 2

17

Zaleznosc miedzy genotypem transferyny [Tf] u kojarzonych loch i knurow a liczebnoscia miotow i segregacja genotypow Tf u potomstwa

70%

Zyczko K.

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1997

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tom 46

83-91

PL

Płodność świń zróżnicowanych typem transferyny (Tf) analizowano na podstawie wyników reprodukcyjnych jednego z gospodarstw zlokalizowanego na terenie woj. olsztyńskiego. W pierwszym etapie badań u 222 loch i 21 knurów rasy wielka biała polska - wbp, określono typ Tf w surowicy krwi metodą elektroforezy w żelu skrobiowym. Ustalono liczbę żywo urodzonego potomstwa w 960 miotach (tj. w 222 - pierwszych i 738 - kolejnych od II do VIII włącznie) po rodzicach o genotypie Tf: AA, BB, AB, kojarzonych w różnych genotypowych układach Tf. Uzyskane wyniki wykazały istotny wpływ typu Tf rodziców na liczbę rodzących się prosiąt w miotach. Zbliżoną liczebnością charakteryzowały się mioty po obojgu rodzicach AA, AB oraz parach AA x AB i AA x BB (odpowiednio: 9.6; 9.4; 9.4 i 9.3 prosięcia w miocie). Mniej liczne były mioty par BB x BB i BB x AB (odpowiednio: 8.3 i 8.9 prosięcia w miocie). W celu wyjaśnienia niekorzystnych efektów reprodukcyjnych par BB x BB i AB x BB, w drugiej części badań przeanalizowano mechanizm segregacji alleli Tf determinujących genotypy potomstwa. Analizą objęto całe mioty loch wieloródek (II - V mioty) o określonych genotypach potomstwa i ich rodziców (wyłącznie z żywo urodzonymi prosiętami, ewentualnie z jednym martwo urodzonym). W obrębie rasy wbp przeanalizowano 60 miotów rodziców kojarzonych z knurami (n = 6) w następujących układach Tf: ♀ AA x ♂ AB (7 miotów, z których uzyskano 56 prosiąt), ♀ AB x ♂ AB (32 mioty - 335 prosiąt), ♀ BB х ♂ AB (14 miotów - 126 prosiąt) i ♀AB x ♂ BB (7 miotów - 78 prosiąt). Po lochach wbp (n = 31) o genotypie Tf AB kojarzonych z knurami (n = 6) rasy polska biała zwisłoucha - pbz ♂ Tf BB, uzyskano 31 miotów, a w nich 318 prosiąt. Otrzymane wyniki wskazały na niesymetryczną segregację alleli w układzie Tf. Efektem tego zjawiska był znaczny niedobór prosiąt o genotypie Tf BB zarówno w potomstwie czysto rasowym, jak i mieszańcowym, zwłaszcza gdy matka nie była homozygotą BB, lecz heterozygotą AB.

EN

Fertility of pigs with different transferrin (Tf) types was analysed on the basis of reproduction results achieved in a farm located in the District of Olsztyn. In the first part of research, including 222 sows and 21 boars of Polish Large White Race, Tf type in blood serum was determined by means of the electrophoresis method in starch gel. The number of piglets born alive in 960 litters (ie in first 222 and further 738 - from the 2nd to the 8th inclusive), originating from parents with the following Tf genotypes: AA, BB, AB, mated in various Tf genotype combinations, was also established. The obtained results indicate a significant effect of parental Tf type on litter size. The sizes of litters with AA, AB, AA x AB and AA x AB parents were similar (ie 9.6; 9.4; 9.4 and 9.3 piglets per litter, respectively). The litters with BB x BB and BB x AB parents were smaller (ie 8.3 and 8.9 piglets per litter, respectively). In order to explain poor reproduction results of BB x BB and AB x BB couples, the segregation mechanism of Tf alleles - which determine progeny genotypes - was examined" in the other part of research. The litters of multiparous sows (2-5 litters), ie with definite genotypes of progeny and their parents (with all piglets born alive, or one born dead), were subjected to analysis. Within the Polish Large White Race, 60 litters of parents mated with boars (n = 6) in the following Tf combinations: Tf ♀ AA x ♂ AB (7 litters, 56 piglets), ♀ AB x ♂ AB (32 litters, 335 piglets), ♀ BB x ♂ AB (14 litters, 126 piglets) and ♀ AB x ♂ BB (7 litters, 78 piglets), were studied. 31 litters (318 piglets) were obtained from PLW sows (n = 31) with Tf AB genotype, mated with Polish Landrace - PL boars (n = 6) with Tf BB genotype. The outcomes of research showed asymmetrical segregation of alleles in Tf combination. It resulted in a considerable shortage of piglets with Tf BB genotype, both in the case of pure breed and crossbreed progeny, especially when the mother was an AB heterozygote, and not a BB homozygote.

18

Oszacowanie dystansu genetycznego u owiec na podstawie polimorfizmu transferyn, hemoglobiny i anhydrazy węglanowej

70%

Bojczuk H. , Bojczuk B. , Zurkowski M.

Zeszyty Problemowe Postępów Nauk Rolniczych

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1988

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tom 352

RU

На основании анализа трех полиформных систем белков ( Hb ,Tf и CA), рассматривая частоту аллелей, коэффициент гомозиготности, коэффициент „чистопородности", коэффициент сродственности и генетическую дистанцию, можно говорить о существенных генетических различиях между сравниваемыми четырьмя популяциями овец: мериносом, низинной овцой и овцой разновидности Желязна.

EN

The analysis of three polymorphic arrangements of proteins (Hb, Tf and CA) and consideration of the frequency of alleles, heterozygocity coefficient, "breed purity" coefficient, relation coefficient and genetic distance proved 1tie existence of significant genetic differences between the four sheep populations compared, viz.: Merino, Lowland, Wielkopolska and Żelazna sheep.

19

Extrahepatic synthesis of acute phase proteins and their functions

70%

Aldred A. R. , Southwell B. , Schreiber G.

Folia Histochemica et Cytobiologica

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1992

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tom 30

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nr 4

20

Badania wiarygodnosci rodowodow owiec w latach 1996-2000

61%

Rychlik T.

Biuletyn Informacyjny. Instytut Zootechniki

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2000

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tom 38

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nr 4

35-42