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EN
Using the total lipid contents and fatty acid profiles, the marine macro-algae Jania rubens (Rhodophyceae), Ulva linza (Chlorophyceae) and Padina pavonica (Phaeophyceae) were evaluated for biodiesel production during the spring, summer and autumn. Seawater parameters such as pH, salinity and temperature were measured. The total lipid content varied from 1.56% (J. rubens) to 4.14% (U. linza) of dry weight, with the highest values occurring in spring. The fatty acid methyl ester profiles were analysed using gas chromatography. The highest percentage of total fatty acids was recorded in P. pavonica, with 6.2% in autumn, whereas the lowest was in J. rubens, with 68.6% in summer. The relative amount of saturated to unsaturated fatty acids was significantly higher in P. pavonica than in the other macro-algae. Seasonal variations in pH, salinity and temperature had no significant effect on the total lipid and fatty acid contents. Principal component analysis grouped brown and green algae together, whereas red alga grouped out. Furthermore, methyl ester profiles indicate that brown and green seaweeds are preferred, followed by red seaweeds, which appears to have little potential for oil-based products. Therefore, these seaweeds are not targets for biodiesel production.
EN
The present study was aimed to develop a cost-effective and efficient protocol for mass propagation of high-quality seedlings through tissue culture by using seaweed extract as biostimulants instead of synthetic chemicals. The nodal explant of field grown W. somnifera estabilised on Murashige and Skoogs medium (MS) and Gamborg B5 medium supplemented with six concentration of 2,4 D. The percentage of culture response from the nodal explant ranged from 44 to 80 and 3.0 mg l-1 2,4 D found to be best for callus induction. MS media containing different concentration of seaweed extract (10, 20, 40, 60, 80 and 100 %) were tested individually for shoot induction. The medium supplemented with 40 % seaweed extract exhibited maximum number of shoots with about 8.6 shoots/ callus and 80 % seaweed extract exhibited 4.3 shoots/ callus. It is evident from this study that seaweed extracts can be used as substitute for synthetic growth hormones for micropropagation of medicinally important plant W. somnifera for clonal propagation and conservation.
EN
Background. According to the European Food Safety Authority (EFSA), fish, seafood and seaweeds are foodstuffs that significantly contribute to dietary arsenic intake. With the exception of some algal species, the dominant compounds of arsenic in such food products are the less toxic organic forms. Both the Joint FAO/ WHO Expert Committee on Food Additives (JECFA) and EFSA recommend that speciation studies be performed to determine the different chemical forms in which arsenic is present in food due to the differences in their toxicity. Knowing such compositions can thus enable a complete exposure assessment to be made. Objectives. Determination of total and inorganic arsenic contents in fish, their products, seafood and seaweeds present on the Polish market. This was then followed by an exposure assessment of consumers to inorganic arsenic in these foodstuffs. Materials and Methods. Total and inorganic arsenic was determined in 55 samples of fish, their products, seafood as well as seaweeds available on the market. The analytical method was hydride generation atomic absorption spectrometry (HGAAS), after dry ashing of samples and reduction of arsenic to arsenic hydride using sodium borohydride. In order to isolate only the inorganic forms of arsenic prior to mineralisation, samples were subjected to concentrated HCl hydrolysis, followed by reduction with hydrobromic acid and hydrazine sulphate after which triple chloroform extractions and triple 1M HCl re-extractions were performed. Exposure of adults was estimated in relation to the Benchmark Dose Lower Confidence Limit (BMDL05) as set by the Joint FAO/WHO Expert Committee on Food Additives (JECFA) that resulted in a 0.5% increase in lung cancer (3.0 μg/kg body weight (b.w.) per day). Results. Mean total arsenic content from all investigated fish samples was 0.46 mg/kg (90th percentile 0.94 mg/kg), whilst the inorganic arsenic content never exceeded the detection limit of the analytical method used (0.025 mg/kg). In fish products, mean total arsenic concentration was 1.48 mg/kg (90th percentile: 2.42 mg/kg), whilst in seafood they were 0.87 mg/ kg (90th percentile: 2.23 mg/kg), for inorganic arsenic contamination at the 90th percentile was 0.043 mg/kg with most results however being less than 0.025 mg/kg. The highest inorganic arsenic levels were determined in the Hijiki algal species samples (102.7 mg/kg), whereas the other algal samples gave a mean inorganic concentration of 0.41 mg/kg (90th percentile 0.86 mg/kg). The estimated average adults exposure to inorganic arsenic in fish, seafood and seaweeds was less than 0.5% of the lowest BMDL0.5 dose. Only for the Hijiki seaweed it was at 4.9% BMDL0.5. Conclusions. Results demonstrate that dietary arsenic intake from fish, seafood and seaweed along with all their products do not constitute a significant health threat to consumers apart from the seaweed species Hizikia fusiformis in which over 40% of all the inorganic arsenic compounds were found.
PL
Wprowadzenie. Ryby, owoce morza i wodorosty morskie należą do grupy środków spożywczych, które według Europejskiego Urzędu ds. Bezpieczeństwa Żywności (EFSA) mogą wnosić istotny udział do pobrania arsenu z dietą. W tej grupie produktów za wyjątkiem niektórych gatunków glonów, dominującymi formami arsenu są mniej toksyczne organiczne połączenia. Zarówno Połączony Komitet Ekspertów FAO/WHO ds. Substancji Dodatkowych do Żywności (JECFA) jak i EFSA wskazują na celowość prowadzenia badań specjacyjnych pozwalających określić różne formy chemiczne, w jakich arsen występuje w żywności, mając na uwadze duże różnice w ich toksyczności. Poznanie informacji o zawartości poszczególnych związków pozwala na dokonanie pełnej oceny narażenia. Cel badań. Oznaczenie zawartości arsenu całkowitego i nieorganicznego w rybach i ich produktach, owocach morza i wodorostach morskich dostępnych w obrocie handlowym w Polsce oraz ocena narażenia konsumentów na pobranie arsenu nieorganicznego z tymi grupami środków spożywczych. Materiał i metody. Analizie na zawartość arsenu całkowitego i nieorganicznego poddano 55 próbek ryb, ich produktów, owoców morza oraz glonów pochodzących z obrotu handlowego. Zawartości arsenu całkowitego i nieorganicznego oznaczono po suchej mineralizacji próbek metodą absorpcyjnej spektrometrii atomowej z wykorzystaniem generacji wodorków (HGAAS), po uprzedniej redukcji arsenu do arsenowodoru za pomocą borowodorku sodu. W celu wydzielenia nieorganicznych form arsenu próbki przed mineralizacją poddano hydrolizie w środowisku stężonego HCl, a następnie redukcji w obecności kwasu bromowodorowego i siarczanu hydrazyny oraz 3-krotnej ekstrakcji chloroformem i reekstrakcji 1M HCl. Oszacowane narażenie w odniesieniu do osób dorosłych porównano z wartością najniższej dawki wyznaczającej (Benchmark Dose Lower Confidence Limit) BMDL0,5 ustalonej przez The Joint FAO/WHO Expert Committee on Food Additives (JECFA) powodującej 0,5%-owy wzrost zachorowań na raka płuc (3,0 μg/kg m.c./dzień). Wyniki. Średnia zawartość arsenu całkowitego w badanych próbkach ryb wyniosła 0,46 mg/kg (90-ty percentyl: 0,94 mg/kg), natomiast arsenu nieorganicznego nie przekraczała granicy wykrywalności stosowanej metody 0,025 mg/kg. W przypadku przetworów rybnych średnie zanieczyszczenie arsenem wyniosło 1,48 mg/kg (90-ty percentyl: 2,42 mg/kg) natomiast owoców morza odpowiednio: 0,87 mg/kg (90-ty percentyl: 2,23 mg/kg), dla arsenu nieorganicznego 90-ty percentyl 0,043 mg/kg przy większości wyników poniżej 0,025 mg/kg. Najwyższym zanieczyszczeniem arsenem nieorganicznym charakteryzowały się glony z gatunku Hijiki (najwyższa zawartość 102,7 mg/kg), w pozostałych próbkach glonów średnia zawartość arsenu nieorganicznego wynosiła 0,41 mg/kg (90-ty percentyl: 0,86 mg/kg). Oszacowane średnie narażenie na arsen nieorganiczny w odniesieniu do osób dorosłych wyniosło dla ryb, owoców morza i wodorostów poniżej 0,5% wartości najniższej dawki wyznaczającej BMDL0,5. Jedynie w przypadku gatunku Hijiki było ono rzędu 4,9 % BMDL0,5. Wnioski. Na podstawie uzyskanych wyników stwierdzono, że pobranie arsenu w wyniku spożycia ryb, ich produktów, owoców morza, oraz wodorostów morskich nie stanowi istotnego zagrożenia dla zdrowia konsumentów poza gatunkiem Hizikia fusiformis zawierającym ponad 40% połączeń nieorganicznych arsenu.
EN
Proximate composition of the three green seaweeds viz., Chaetomorpha aerea, Enteromorpha intestinalis, Enteromorpha compressa one brown seaweed Dictyota dichotoma and one red seaweed Gracilaria corticata was investigated by determination of protein, carbohydrates, lipid, moisture and ash content. In the present study, the total protein was recarded upper most in E. intestinalis and bare minimum in E. compressa. The maximum carbohydrates recorded in C. aerea and minimum in G. corticata. The lipid content was acquired upper limit in E. compressa and least in C. aerea. The ash and moisture content in following of 5 different seaweeds are as follows: 7.45mg/g, 3.91mg/g were recorded in the C. aerea, 8.52mg/g, 3.42mg/g in the E. intestinalis, 8.58mg/g, 3.75mg/g in the E. compressa, 9.47mg/g, 4.23mg/g in the D. dichotoma and 6.95mg/g, 3.98mg/g in G. corticata. The proximate composition of 5 different seaweed species exhibited high nutritional value for human consumption.
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EN
With the rising awareness of the adverse effects of chemical pesticides, people are looking for organically grown vegetables. Consumers are increasingly choosing organic foods due to the perception that they are healthier than those conventionally grown. Vegetable crops are vulnerable to a range of pathogenic organisms that reduce yield by killing the plant or damaging the product, thus making it unmarketable. Soil-borne diseases are among the major factors contributing to low yields of organic produce. Apart from chemical pesticides there are several methods that can be used to protect crops from soil-borne pathogens. These include the introduction of biocontrol agents against soil-borne plant pathogens, plants with therapeutic effects and organic soil amendments that stimulate antagonistic activities of microorganisms to soil-borne diseases. The decomposition of organic matter in soil also results in the accumulation of specific compounds that may be antifungal or nematicidal. With the growing interest in organic vegetables, it is necessary to find non chemical means of plant disease control. This review describes the impact of soil-borne diseases on organic vegetables and methods used for their control.
PL
W artykule przedstawiono informacje dotyczące potencjalnego ryzyka dla zdrowia związanego ze spożyciem wodorostów morskich z uwzględnieniem stwierdzanego poziomu zanieczyszczenia pierwiastkami szkodliwymi dla zdrowia. Oceny dokonano na podstawie wyników badań własnych oraz danych zgromadzone przez państwa członkowskie UE. Omówiono aktualny stan prawny w tym obszarze oraz działania podjęte przez Komisję Europejską zmierzające do właściwego zarządzania ryzykiem. Zwrócono również uwagę na powiadomienia zgłaszane w ramach systemu Wczesnego Ostrzegania o Niebezpiecznej Żywności i Paszach (RASFF), dotyczące stwierdzenia istotnego zagrożenia dla zdrowia związanego z wysoką zawartością metali ciężkich w wodorostach morskich i suplementach diety je zawierających.
EN
The article presents information on the potential health risk related to the consumption of seaweed, taking into account the level of contamination with elements harmful to human health. The assessment was performed on the basis of own results as well as the results collected by the European Union Member States. The current legal status in this area as well as the actions undertaken by the European Commission aimed to proper risk management were also discussed. Attention was also paid to notifications reported under the Rapid Alert System for Food and Feed (RASFF) regarding a significant health risk related to the high content of heavy metals in seaweed and food supplements containing them.
EN
Heavy metals are major stressors for benthic macroalgal communities in marine ecosystems. In this study, the effects of copper and cadmium on some physiological parameters along with antifouling defense of the marine macroalga Ulva reticulata were assessed under laboratory conditions. Macroalgal samples were treated with three concentrations (1 mg l−1, 3 mg l−1 and 5 mg l−1) of copper and cadmium for 2 and 7 days. After treatment, algal samples were analyzed for chlorophyll-a, carotenoid, total polyphenol and total antioxidant capacity. Also, algal extracts were tested against biofilm-forming bacteria strains to understand differences in antifouling activity. The results indicated that exposure of U. reticulata to copper and cadmium, on the one hand, induced protective mechanisms such as total phenol production and antioxidant capacity against metal stress and, on the other hand, reduced photosynthesis. While the extract obtained from control algal samples showed a strong inhibitory effect on the growth of biofilm-forming bacteria, treatment with heavy metals resulted in reduced antibiofilm activity. In general, the results revealed that exposure of macroalgae to heavy metals can affect antifouling defense traits in addition to changes in photosynthetic pigment content.
EN
Macroalgae are one of the potential natural sources for the isolation of novel eco-friendly antifouling compounds. In this study, the antifouling activity of an extract of the brown macroalga Dictyota dichotoma collected from the Red Sea was tested against bacteria isolated from the marine biofilm and larval forms of the barnacle. A maximum inhibition of barnacle larval settlement of 89.36% was observed in 25 µg ml-1 extract concentration at 24 h treatment. The secondary metabolite composition of the extract was analyzed by GC-MS and compounds were used as ligands for molecular docking with barnacle cement protein. The toxicity profile of secondary metabolites present in the extract was predicted through in silico analysis. The results indicate that the crude extract of the alga inhibited the biofilm formation by the bacteria and significantly reduced the settlement of the barnacle larvae. GC-MS analysis of the extract revealed the presence of five metabolites, including two fatty acids. All metabolites showed higher binding affinity with barnacle cement than the reference compound, copper. Among the secondary metabolites detected in the algal extract, the cholestane derivative exhibited maximum binding affinity (–14.2 kcal mol-1) with barnacle cement. The metabolites also showed positive for crustacean and fish toxicity in toxicity prediction using an in silico method.
EN
The aim of the experiments carried out in a high, polyethylene in the years 2004- -2006 was to examine the influence of the algae preparation Bio-algeen S-90 on yield, mineral composition of fruits, content of assimilatory pigments, intensity of assimilation and transpiration and photosynthetic water use efficiency index of the cherry tomato cv. Conchita F1. The results on tomato yields proved significant differences in total and marketable yield under the influence of Bio-algeen. The biggest total and marketable yields were obtained when plants were treated three times with Bio-algeen. Bio-algeen also caused increase in content of mineral components in cherry tomato fruits. Three- and fourfold sprays increased the content of total nitrogen, phosphorus, potassium, calcium, zinc, iron and nitrates. Treating tomato plants with the preparation increased the content of assimilatory pigments of tomato. Increase in a number of Bio-algeen sprays led to higher contents of total, a and b chlorophyll and carotenoids in leaves. Intensity of assimilation and transpiration of the plants and water use efficiency index depended on number of plants spraying with Bio-algeen. Plants sprayed two and three times with Bio-algeen were characterized by greater transpiration and assimilation intensity, but also by smaller efficiency of water use index.
PL
Celem badań prowadzonych w wysokim tunelu foliowym, w latach 2004-2006, było określenie wpływu stosowania preparatu z alg morskich Bio-algeen S-90 na plon, skład mineralny owoców, zawartość barwników asymilacyjnych, intensywność asymilacji i transpiracji oraz wskaźnik efektywności wykorzystania wody w procesie fotosyntezy roślin pomidora drobnoowocowego odmiany Conchita Fj. Wykazano istotne różnice w plonie ogółem i handlowym pod wpływem traktowania roślin preparatem Bio-algeen S-90. Największy plon ogółem i handlowy owoców uzyskano w przypadku 3-krotnego oprysku preparatem. Preparat Bio-algeen wpłynął również na zwiększenie zawartości składników mineralnych w owocach pomidora drobnoowocowego. Opryskiwanie 3- i 4-krotne roślin zwiększyło po ziom azotu ogólnego, fosforu, potasu, wapnia, cynku, żelaza oraz azotanów. Traktowanie roślin preparatem zwiększyło zawartość barwników asymilacyjnych u pomidora. Zwiększanie liczby oprysków preparatem Bio-algeen wpłynęło na wyższą zawartość chlorofilu a, b i całkowitego oraz karotenoidów w liściach. Intensywność asymilacji i transpiracji roślin oraz wskaźnik efektywności wykorzystania wody zależał od liczby oprysków preparatem Bio- algeen. U roślin, które opryskiwano 2- i 3-krotnie, stwierdzono większą intensywność transpiracji i asymilacji, ale mniejszy wskaźnik efektywności wykorzystania wody.
EN
The objective of our study was to determine changes in protein and monosaccharide content in select grass species due to an application of the kelp species Ecklonia maxima extract. The field experiment was arranged as a randomized sub-block design (split-split-plot) with three replicates. The following factors were examined: growth stimulant with the trade name Kelpak SL, which was either applied at the rate of 2,000 dm3·ha-1 or not applied, pure-sown grass species, and cultivars (Dactylis glomerata, cv. Amila and Tukan; Festulolium braunii, cv. Felopa and Agula). Protein compounds and monosaccharides were determined in the dry matter of the tested grasses. The biostimulant Kelpak significantly increased both protein compounds and monosaccharides as well as the carbohydrate-protein ratio in the plants. The grass species and their cultivars had different levels of protein compounds and carbohydrates.
EN
Seaweed extracts have been recently introduced to crop growing, particularly to sustainable agriculture, in many countries worldwide. However, our knowledge of the action produced by Kelpak is only fragmentary as it is influenced by a number of factors, for example crop plant species and extract application schedule. Our objective was to determine the effect of Kelpak SL on the content of selected microelements in two grass species. A field experiment was arranged in a randomized subblock design (split-split-plot) with three replicates. It was conducted at the Experimental Unit of the University of Natural Sciences and Humanities in Siedlce (Poland) and started in late April each year. The following factors were examined: pure stands of two grass species Dactylis glomerata L. (cv. Amila) and Festulolium braunii (K.Richt.) A. Camus (cv. Felopa) grown in a monoculture, a biostimulant distributed under the trade name Kelpak SL, applied at 2 dm3 ha-1 (no biostimulant in the control treatment), and nitrogen applied at 50, 100, 150 kg ha-1 (no nitrogen in the control). During the experiment, grass was cut three times a year. The plant material was subjected to chemical analyses to assess dry matter (by determining the moisture content), zinc, copper, iron and manganese. The application of Kelpak significantly increased the Zn, Cu, Fe and Mn content in the grass species tested, regardless of the remaining factors. The grass species did not differ significantly in their content of Zn, Cu, Fe and Mn. The concentrations of the microelements in both species were significantly affected by a dose of nitrogen. At higher nitrogen doses, the concentrations of Zn, Cu and Fe were lower, unlike the content of Mn, which increased. The Fe:Mn ratio in the dry matter of both grasses was 2.79, which indicates some manganese deficiency.
EN
Seaweeds are one of the important marine bioresources which are nowadays termed as eco-friendly stimulators of crop growth, stress tolerance, and yielding. In this review, we give an update of the current state of our understanding of the seaweed extracts (SWE) effects on the physiological alterations they induce in Solanaceae vegetables. SWE may provide a powerful and environmentally friendly approach to nutrient management. A pool of common bioactive molecules of SWE provides enhancement of the antioxidant machinery of treated plants providing balanced development, earlier flowering, and enhanced fruiting. The basic mechanisms of SWE action seem to be unspecific for tomato, pepper, and eggplant. They include accelerating growth, nutrient uptake, and photosynthesis performance, which can induce plant tolerance to adverse environmental conditions, improve flowering, fruit setting, and yield, and enhance nutritional quality of the crops. The mechanism of SWE action is complex because of multielement composition and cross-action between constituents.
PL
Algi morskie są elementem zasobów biologicznych mórz i oceanów. Obecnie wykorzystuje sie je w rolnictwie jako naturalne stymulatory wzrostu, tolerancji na stres i plonowania roślin uprawnych. Niniejsze opracowanie przedstawia aktualny stan wiedzy dotyczący mechanizmów działania i efektów stosowania ekstraktów z alg morskich (EAM) w uprawie warzyw z rodziny Solanaceae (pomidora, papryki i oberżyny). Bioaktywne składniki EAM regulują status mineralny roślin, modyfikują gospodarkę hormonalną, regulują rozwój wegetatywny i generatywny. Mechanizmy działania EAM wydają się być niespecyficzne dla pomidora, papryki i oberżyny. U wszystkich wymienionych gatunków zaobserwowano efektywniejsze pobieranie składników pokarmowych z gleby, lepszą wydajność fotosyntezy i przyspieszony wzrost. Te zmiany mogą z kolei wpływać pozytywnie na tolerancję roślin w stosunku do niekorzystnych warunków środowiskowych, intensyfikować kwitnienie i zawiązywanie owoców, zwiększać plon oraz poprawiać jego jakość. Ze względu na kompleksowy skład chemiczny i interakcje między składnikami, mechanizmy działania EAM są wielokierunkowe i trudne do zanalizowania na podstawie wyników doświadczeń rolniczych.
EN
In this study, 12 taxa from the Chlorophyta, Phaeophyta and Rhodophyta were collected from different depths at Gemlik-Karacaali and Erdek-Ormanlı. A total of 175 specimens from these divisions were used to determine Total Protein (TP), Total Soluble Carbohydrate (TSCH) and Chlorophyll a (Chl a), Chlorophyll b (Chl b), Chlorophyll c (Chl c), total carotenoid (Car) contents and Chl b/Chl a, Chl c/Chl a, Car/Chl a, Car/Chl b, Car/Chl c ratios. TP, TSCH and pigment contents varied significantly with respect to the algal taxa, stations and depth distribution. In addition, individual differences were important in all of the measured parameters. The maximum TP contents (0.94%–31.03%) were determined in some of the Rhodophyta. In some green seaweeds belonging to the genus Ulva L., the TP content was determined between 2.9%–28.1%. Lower TP contents were determined in Cystoseira barbata (Good) C. Agardh (1.1%–4.3%). In contrast to TP contents, TSCH values were very low; maximum TSCH were determined in Ulva species, as were protein contents. In conclusion, the variations in TP, TSCH and pigment in 12 taxa of macroalgae were analysed according to station, depth, and environment.
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