Wyniki wyszukiwania - Biblioteka Nauki

1

Ripening of rennet cheeses with different content of salt

100%

Wisniewska K. , Reps A. , Jarmul I. , Iwanczak M.

Natural Sciences

|

1999

|

tom 03

2

Hem12, an enzyme of heme biosynthesis pathway, is monoubiquitinated by Rsp5 ubiquitin ligase in yeast cells

86%

Chelstowska A. , Jastrzebska Z. , Kaminska J. , Sadurska A. , Plochocka D. , Rytka J. , Zoladek T.

Acta Biochimica Polonica

|

2015

|

tom 62

|

nr 3

509-515

EN

Heme biosynthesis pathway is conserved in yeast and humans and hem12 yeast mutants mimic porphyria cutanea tarda (PCT), a hereditary human disease caused by mutations in the UROD gene. Even though mutations in other genes also affect UROD activity and predispose to sporadic PCT, the regulation of UROD is unknown. Here, we used yeast as a model to study regulation of Hem12 by ubiquitination and involvement of Rsp5 ubiquitin ligase in this process. We found that Hem12 is monoubiquitinated in vivo by Rsp5. Hem12 contains three conserved lysine residues located on the protein surface that can potentially be ubiquitinated and lysine K8 is close to the 36-LPEY-39 (PY) motif which binds WW domains of the Rsp5 ligase. The hem12-K8A mutation results in a defect in cell growth on a glycerol medium at 38°C but it does not affect the level of Hem12. The hem12-L36A,P37A mutations which destroy the PY motif result in a more profound growth defect on both, glycerol and glucose-containing media. However, after several passages on the glucose medium, the hem12-L36A,P37A cells adapt to the growth medium owing to higher expression of hem12-L36A,P37A gene and higher stability of the mutant Hem12-L36A,P37A protein. The Hem12 protein is downregulated upon heat stress in a Rsp5-independent way. Thus, Rsp5-dependent Hem12 monoubiquitination is important for its functioning, but not required for its degradation. Since Rsp5 has homologs among the Nedd4 family of ubiquitin ligases in humans, a similar regulation by ubiquitination might be also important for functioning of the human UROD.

3

Ribophagy - the novel degradation system of the ribosome

86%

Bakowska-Zywicka K. , Tyczewska A.

Biotechnologia

|

2009

|

nr 1

99-103

4

Characterization of the biomethanization process in selected waste mixtures

72%

Montusiewicz A. , Lebiocka M. , Pawłowska M.

Archives of Environmental Protection

|

2008

|

tom Vol. 34, no. 3

49-61

EN

The aim of this paper is to show the basic principles of the anaerobic digestion process. All the stages of degradation, such as hydrolysis, acidogenesis, acetogenesis and methanogenesis are characterized. Biodegradable organic matter consists of three main types of substances: carbohydrates, proteins and lipids; the metabolic pathways of their decomposition are described. The last part of the paper presents the co-digestion process, its benefits and technological parameters required to mate that process attractive from an economical and environmental point of view.

5

Crosstalk between autophagy and proteasome protein degradation systems: possible implications for cancer therapy

72%

Wojcik S.

Folia Histochemica et Cytobiologica

|

2013

|

tom 51

|

nr 4

6

Ocena podatności wybranych białek mleka na degradację proteazą serynową dyni figolistnej Cucurbita ficifolia

72%

Dąbrowska A. , Babij K. , Szołtysik M. , Pokora M. , Zambrowicz A. , Chrzanowska J. , Kupczyński R.

Przemysł Chemiczny

|

2013

|

tom T. 92, nr 5

740-745

7

Ocena podatności wybranych białek mleka na degradację proteazą serynową dyni figolistnej Cucurbita ficifolia

72%

Dąbrowska A. , Babij K. , Szołtysik M. , Pokora M. , Zambrowicz A. , Chrzanowska J. , Kupczyński R.

Przemysł Chemiczny

|

2013

|

tom T. 92, nr 5

740-745

8

Biologically active peptides derived from food proteins

72%

Kostyra H. , Kostyra E.

|

nr 4

9

Effect of 3-hydroxy-3-methylbutyrate [HMB] on muscle cathepsins and calpain activities during the post-dexamethasone recovery period in young rats

72%

Jank M. , Ostaszewski P. , Rosochacki S. , Wilczak J. , Balasinska B.

|

nr 4

EN

This study was conducted to assess the effect of the leucine metabolite, 3-hydroxy-3-methylbutyrate (HMB) on animal performance, and also cathepsins and calpain II activities in the gastrocnemius muscle in young rats undergoing dexamethasone (DX) treatment and subsequent recovery. Five days of DX administration resulted in an increase in calpain activity. During 5 days of recovery alone, calpain activity was still elevated whereas HMB treatment decreased calpain activity to the values observed in the control group. DX treatment increased the total lysosomal proteolytic activity. HMB administration during the recovery period accelerated return of the proteolytic enzymes activity to the control values. The use of selective inhibitors of thiol and aspartic cathepsins (leupeptin and pepstatin, respectively) allowed us to determine the type of cathepsin responsible for the DX-induced proteolysis observed. Since DX treatment decreased cathepsin D activity (which returned to the control values during recovery) it is assumed that thiol cathepsins are involved in the increase of lysosomal proteolysis observed. We have demonstrated that lysosomal and Ca+2-dependent proteinases involved in myofibryllar protein degradation differ in their activity due to DX treatment. It has been concluded that HMB modifies muscle proteolysis through changes in the activity of the proteolytic enzymes. Practical applications of this phenomenon are discussed.

10

The effects of wilting and additives on silage quality, protein degradation in the silo and in the rumen, and dairy cattle productivity

72%

Gasior R. , Brzoska F.

Roczniki Naukowe Zootechniki

|

2000

|

tom 27

|

nr 4

11

Wzrost drozdzy w modelowym serze i ich wplyw na degradacje bialek i tluszczu

58%

Czajgucka A. , Chrzanowska J. , Juszczyk P. , Szoltysik M. , Polomska X. , Wojtatowicz M.

Acta Scientiarum Polonorum. Biotechnologia

|

2006

|

tom 05

|

nr 1-2

95-103

12

Effect of gibberellic acid and commercial conditioners on senescence of cut shoots of Asparagus densiflorus 'Meyerii'

58%

Rabiza-Swider J. , Skutnik E.

Annals of Warsaw University of Life Sciences - SGGW. Horticulture and Landscape Architecture

|

2009

|

tom 30

13

Wplyw zawartosci soli oraz czesciowego zastapienia sodu potasem na proces degradacji bialek w serach dojrzewajacych

58%

Iwanczak M. , Wisniewska K. , Reps A. , Dajnowiec F.

Acta Academiae Agriculturae ac Technicae Olstenensis. Technologia Alimentorum

|

1998

|

tom 30

23-33

14

Udzial tioredoksyny w przemianach metabolicznych roslin wyzszych, a szczegolnie w syntezie i degradacji bialek zapasowych ziarniakow zboz

58%

Kaczkowski J.

Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin

|

2001

|

tom 217

5-18

15

Senescence control of cut shoots of Asparagus setaceus

58%

Skutnik E. , Rabiza-Swider J.

Annals of Warsaw University of Life Sciences - SGGW. Horticulture and Landscape Architecture

|

2009

|

tom 30

16

Effect of plant growth regulators and preservative solutions on senescence of detached Hosta leaves

58%

Skutnik E. , Rabiza-Swider J.

Annals of Warsaw University of Life Sciences - SGGW. Horticulture and Landscape Architecture

|

2007

|

tom 28

17

Changes in chemical composition of meat from roe deer [Capreolus capreolus L.] bucks during cold storage under vacuum and modified atmosphere

58%

Daszkiewicz T. , Janiszewski P. , Kondratowicz J.

Polish Journal of Food and Nutrition Sciences

|

2008

|

tom 58

|

nr 3

EN

Changes in the chemical composition, nitrogen content of water extracts, weight loss and shear force of meat from roe deer (Capreolus capreolus L.) bucks were analysed during 21-day cold storage (2°C) under modified atmosphere conditions (vacuum; 40% CO2 + 60% N2; 60% CO2 + 40% N2). A gradual increase in drip loss was observed over the storage period. The process of cold storage of roebuck meat in a modified atmosphere (MA) had no significant effect on the content of major chemical components. The dynamics of changes in the content of nitrogen compounds in water extracts of cold-stored meat was lower in MA-packaged samples, compared to the vacuum-packaged samples. The relatively low rate of protein degradation (proteolysis) in roebuck meat during ageing was reflected in the lack of considerable changes in the average values of shear force.

18

Effect of endotoxin on protein degradation and lipid peroxidation of erythrocytes

58%

Bhattacharyya J. , Chowdhury T. D. , Datta A. G.

Journal of Physiology and Pharmacology

|

1999

|

tom 50

|

nr 2

19

Fluorogenic peptide substrates for carboxydipeptidase activity of cathepsin B

58%

Stachowiak K. , Tokmina M. , Karpinska A. , Sosnowska R. , Wiczk W.

Acta Biochimica Polonica

|

2004

|

tom 51

|

nr 1

EN

Cathepsin B is a lysosomal cysteine protease exhibiting mainly dipeptidyl carboxypeptidase activity, which decreases dramatically above pH 5.5, when the enzyme starts acting as an endopeptidase. Since the common cathepsin B assays are performed at pH 6 and do not distinguish between these activities, we synthesized a series of peptide substrates specifically designed for the carboxydipeptidase activity of cathepsin B. The amino-acid sequences of the P5-P1 part of these substrates were based on the binding fragments of cystatin C and cystatin SA, the natural reversible inhibitors of papain-like cysteine protease. The sequences of the P'1-P'2 dipeptide fragments of the substrates were chosen on the basis of the specificity of the S'1-S'2 sites of the cathepsin B catalytic cleft. The rates of hydrolysis by cathepsin B and papain, the archetypal cysteine protease, were monitored by a continuous fluorescence assay based on internal resonance energy transfer from an Edans to a Dabcyl group. The fluorescence energy donor and acceptor were attached to the C- and the N-terminal amino-acid residues, respectively. The kinetics of hydrolysis followed the Michaelis-Menten model. Out of all the examined peptides Dabcyl-R-L-V-G-FE(Edans) turned out to be a very good substrate for both papain and cathepsin B at both pH 6 and pH 5. The replacement of Glu by Asp turned this peptide into an exclusive substrate for cathepsin B not hydrolyzed by papain. The substitution of Phe by Nal in the original substrate caused an increase of the specificity constant for cathepsin B at pH 5, and a significant decrease at pH 6. The results of kinetic studies also suggest that Arg in position P4 is not important for the exopeptidase activity of cathepsin B, and that introducing Glu in place of Val in position P2 causes an increase of the substrate preference towards this activity.

20

Wpływ stopnia ubicia sianokiszonki z lucerny na rozkład suchej masy i białka w żwaczu owiec

44%

Kowalski Z. M. , Kaminski J. , Krawontka J.

Acta Agraria et Silvestria. Series Zootechnica

|

1989

|

tom 28