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1 Polish Journal of Food and Nutrition Sciences

1 2018

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Comparison of the biological activity of crude polysaccharide fractions obtained from Cedrela sinensis using different extraction methods

100%

Oh M. H. , Yoon K. Y.

nr 4

Four preparations of crude polysaccharides were obtained from Cedrela sinensis using four methods of extraction, and finally precipitation with ethanol. Hot-water and processes assisted by ultrasound and enzymes (Shearzyme or Viscozyme) were carried out. The yield of polysaccharide fraction extracted with the enzyme-assisted method using Shearzyme (P-EAS) was the highest at 10.53%. All crude saccharides were composed of six monosaccharides including arabinose, galactose, glucose, mannose, rhamnose, and xylose. Crude polysaccharide fraction extracted by hot water showed the highest DPPH• and ABTS•+ scavenging activities as well as α-amylase inhibitory activity. All fractions showed higher retardation effects on glucose and bile acid absorption using the in vitro dialysis system compared to the control (without polysaccharide fraction); specifically, the glucose retardation index of P-EAS was similar to that of carboxymethylcellulose. Overall, crude polysaccharide fractions obtained from C. sinensis can be used as functional ingredients owing to their excellent functional properties.