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EN
The manuscript describes an easy method of isolation of plant genomic DNA. This method allowed us to isolate substantial amounts of good quality DNA from lupin (Lupinus luteus) tissues. The described method also appeared to be useful for genomic DNA isolation from tissues of other plants.
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Content available 65 years of in vitro culture in Poland
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EN
This paper is a short review of historical development of the tissue culture method in Poland. Similarly to the rest of the world, in vitro technology in Poland has progressed in many directions simultaneously. Its main fields are closely interconnected by natural sequences of biological processes and integrade one into another. The best results, driven by the prospects of practical applications, have been achieved within the areas of meristem culture and micropropagation of valuable genotypes, somaclonal variation, production of haploids and DH plants, somatic embryogenesis, in vitro culture of embryos and ovules, improvement of plant disease resistance, somatic hybridization and transformation of plants. Due to the fact that in vitro domain is a very broad science the authors are aware that this review might not fully cover all the scientists working with tissue culture and their achievements.
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This paper discusses transfer cell wall deposition and architecture in various trap hairs of the carnivorous plant Utricularia intermedia. Scanning electron microscopy showed that the middle cells of both internal hairs and pavement epithelium hairs have reticulate-type wall ingrowths. The wall ingrowths of the middle cell of both quadrifids and bifids are very well developed. However, in middle cells of pavement epithelium hairs the level of development of wall ingrowths is not uniform. The presence of ruptured cuticles and wall ingrowths in these hairs suggests that water is transported by the pavement epithelium hairs from the trap to the external environment.
PL
Potwierdzeniem zmian fizycznych przebiegających w materiale w czasie suszenia jest tworzenie przestrzeni o większym polu przekroju, na skutek mechanicznego zniszczenia struktur wewnętrznych. Świadczy to o destrukcyjnym działaniu występującego w czasie suszenia skurczu i towarzyszących mu naprężeń. Zaobserwowane zmiany strukturalne zależą przede wszystkim od rodzaju suszonego materiału, co może być związane między innymi ze zróżnicowanym składem chemicznym.
EN
Creation of spaces with larger surface area made by mechanical destruction of internal structures, among others cell walls, is the confirmation of physical changes occurring in plant materials during drying. It testifies that shrinkage and stresses appearing in the process of drying play destructive role in it. Observed structural changes mainly depend on kind of dried material, what can be in connection with, among others, different chemical constitution.
EN
The study of plant-virus interactions requires a method which enables not only the detection of virus presence, but also its distribution in the infected plant tissue. We adopted a tissue print immunoblot technique for localization of potato virus Y (PVY) coat protein (CP) in infected potato and tobacco tissue. In potato stem cross-sections PVY CP was detected on the whole surface of the prints, however significantly higher concentration was observed in epidermis and phloem tissue. In the petiole of infected tobacco leaves the presence of viral protein was confined mainly to peripheral layers of parenchyma and epidermis. In phloem tissue the signal was also visible but it was significantly weaker. In our hands this approach is highly specific and gives resolution on the level of individual cells, thus it can be applied in several fields. As potyviral capsid proteins are involved in several events of pathogenesis the technique for immunolocalization of PVY CP could provide information which will shed new light on the virology of PVY.
EN
As a result of plant tissue deformation irreversible physical processes that finally lead to the state of resistance, take place. One of these irreversible processes is cracking of the cell structure that occurs through cell wall breaking and losing cohesion in the layer of pectin lamella. As a result of these phenomena a signal of acoustic emission is emitted. In the present study the method of acoustic emission and its application in studying the processes of plant tissue cracking are described.
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