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RU
Цель работы состояла в сравнении результатов, полученных с применением теста „Lactocult”, с результатами, полученными при помощи микробиологических методов, обязывающих в настоящее время. Исследования провели на 50 пробах молочного сырья, определяя общее число кислородных микроорганизмов, титр и число палочек из группы ободочной кишки, а также число оксидаза-положительных микроорганизмов. Тест „Lactocult”, из-за простоты применения и значительного сокращения времени исследований кажется особенно пригодным в случае микробиологических рутиновых исследований, целью которых было бы обнаружение, не превысило ли число исследуемых бактерий уровня, определенного требованиями. Сравнивая полученные результаты при помощи тесте t-Стъюдента, получили следующие коэффициенты корреляции: для общего числа кислородных микроорганизмов — 0,769, в случае палочек из группы ободочной кишки — 0,822, а также в случае оксидазо-положительных микроорганизмов — 0,574. Полученные результаты статистически существенны.
EN
The objective of the work was to compare the results obtained in the Lactocult test and these obtained during ccmmonly used microbiological examinations. The examinations were done with 50 samples of raw milk material in which a total number of aerobic bacteria, number and index of bacteria from Coli group and number of oxidase-positive bacteria was determined. The test due to its simplicity and shortening of examination time may be especially useful in the routine microbiological examinations to find if the number of bacteria in a examined sample is in normal limits. Comparing the obtained results by the T-Student test the following correlation coefficients were found: for a general number of aerobes — 0.769 for Coli group — 0.822, for oxidase-positive bacteria — 0.574. These results differ statistically significantly.
EN
The aim of the study was to determine the efficacy of acidified sodium chlorite (ASC), chlorine dioxide and trisodium phosphate (TSP) in the reduction of contamination levels regarding total microbial count, coliform bacteria, Staphylococcus aureus and Salmonella Enteritidis in chicken carcasses after a decontamination process. Experimental material comprised skin sections from the neck collected from chickens on the production line. Each sample consisted of skin sections coming from three carcasses, each at approx. 20 g. Next each section was divided into two parts, one being the control and the other was the tested sample. Samples were decontaminated by immersion in solutions of analyzed antimicrobial compounds. Control samples and samples after decontamination were subjected to microbiological analysis in which total microbial count, coliform bacteria and Staphylococcus aureus were determined in accordance with the standards PN-EN ISO 4833:2004, PN-ISO 4831:1998, PN-EN-ISO 6888-1:2001 and PN-EN-ISO 6888-1:2001/A:2004, while Salmonella Enteritidis was determined according to the recommendations of the manufacturer of the Chromagar Salmonella Plus medium. An effective antibacterial effect of the applied compounds was found in the limitation of bacterial counts on chicken carcasses. The greatest reduction of total bacterial count, amounting to approx. 25%, was shown when applying acidified sodium chlorite. Trisodium phosphate was the most effective agent against coliform bacteria, reducing their number by 92%. The best antibacterial effect in relation to Staphylococcus aureus was obtained using chlorine dioxide. A 44% reduction of the count of Salmonella Enteritidis was observed for all the applied compounds. A considerable decrease in the level of microbial contamination in poultry carcasses, including pathogens, effectively enhances the standard of health safety in poultry meat.
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nr 03
327-331
EN
The purpose of the study was to determine the effects of the interaction between methods of udder and teat cleaning before milking, the sequence of fore-stripping relative to udder and teat cleaning, and post-milking teat disinfection on somatic cell count and total microorganism count in bulk tank milk. The study was carried out in 2004 on 160 dairy farms (average 19.4 cows per herd), located in neighboring parts of the Mazowsze and Warmia and Mazury regions. It was found that the optimal combination of pre- and post-milking procedures is: fore-stripping, then pre-milking udder and teat wiping with a towel soaked with a disinfectant, and post-milking teat disinfection. However, in very dirty cows the most favorable combination is washing with water containing a disinfectant, followed by fore-stripping. In this case post-milking teat disinfection should not be employed, for the joint effect of this procedure and the specified method of pre-milking udder cleansing is negative in cold months (January-March and September-December), with no effect in other months.
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