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Could AGEs be markers of pre-eclampsia? Study of concentration of advanced protein glycation products in pregnant women with physiological pregnancy and pregnancy complicated with pre-eclampsia

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Bodzek P. , Janosz I. , Damasiewicz-Bodzek A. , Witek Ł. , Olejek A.

Annales Academiae Medicae Silesiensis

2022

nr 76

106-111

INTRODUCTION: The role of advanced glycation end products (AGEs) in the pathomechanism of arterial hypertension has been demonstrated, but little information is available on the influence of AGEs on the course of pre-eclampsia (PE). The aim of the study was to assess the concentration profile of advanced protein glycation products in pregnant women diagnosed with PE. MATERIAL AND METHODS: The concentrations of AGEs, carboxymethyllysine (CML), carboxyethyllysine (CEL) and methylglyoxal (MG) in the sera of female respondents were determined using the enzyme immunoassay method. RESULTS: The levels of AGE and CML were lower in the group of women with PE compared to the group of non- -pregnant women (p = 0.0411 and p = 0.0072). A lower CML concentration was found in healthy pregnant women as compared to healthy non-pregnant women (p = 0.00068). Positive correlations were found between AGE and CML levels in women with PE (R = 0.339, p = 0.032) and between CML and CEL in healthy non-pregnant women (R = 0.447, p = 0.012). CONCLUSIONS:We suggest that there is a decrease in the intensity of non-enzymatic protein glycation during pregnancy. Moreover, our study indicates that isolated PE may be associated with a different pathomechanism than chronic hypertension, and therefore AGEs cannot be at present considered a marker of PE.

WSTĘP: Rola końcowych produktów zaawansowanejglikacji (advanced glycation end products – AGEs) w patomechanizmie nadciśnienia tętniczego została udowodniona, jednak niewiele jest informacji na temat wpływu AGEs na przebieg stanu przedrzucawkowego (pre-eclampsia – PE). Celem pracy była ocena profilu stężeń zaawansowanych pro-duktów glikacji białek u kobiet ciężarnych z rozpoznaniem PE. MATERIAŁ I METODY: Oznaczenia stężeń AGEs, karboksymetylolizyny (carboxymethyllysine – CML), karboksyetylolizyny (carboxyethyllysine – CEL) i metyloglioksalu (methylglyoxal – MG) w surowicy badanych kobiet wykonano metodą immunoenzymatyczną. WYNIKI: Stężenia AGE i CML były niższe w grupie kobiet z PE w porównaniu z grupą kobiet nieciężarnych (p = 0,0411 i p = 0,0072). Stwierdzono niższe stężenie CML u zdrowych ciężarnych w porównaniu ze zdrowymi nieciężarnymi (p = 0,00068). Stwierdzono dodatnie korelacje pomiędzy stężeniem AGE i CML u kobiet z PE (R = 0,339, p = 0,032) oraz pomiędzy CML i CEL u zdrowych kobiet nieciężarnych (R = 0,447, p = 0,012). WNIOSKI: Sugerujemy, że w czasie ciąży dochodzi do zmniejszenia intensywności nieenzymatycznej glikacji białek. Ponadto nasze badanie wskazuje, że izolowane PE może być związane z innym patomechanizmem niż przewlekłe nadciśnienie tętnicze i dlatego AGEs nie mogą być obecnie uważane za marker PE.

Effect of modifications of pea albumins on their immunoreactive properties

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Mierzejewska D. , Chudzik-Kozlowska J. , Kostyra H.

Polish Journal of Food and Nutrition Sciences

2008

tom 58

nr 2

The aim of this study was to investigate the effect of non-enzymatic glycosylation and hydrolysis of pea albumins with pepsin on their immunoreactive properties. Albumin fraction was isolated from pea seeds an then glycated and hydrolysed by pepsin. Pea albumin was characterised by SDS‑PAGE and glycotest. A 15% progress in non-enzymatic glycosylation was found. The in vivo experiment demonstrated the influence of glycation on mouse mucosal immune system. The influence of native, glycated and hydrolysed pea albumins on spleen (SPL) and mesenteric lymph nodes (MLN) lymphocytes proliferation was investigated. The culture MLN lymphocytes showed an increase in proliferation during stimulation with all of antigens (native, glycated, hydrolyzed). The proliferation was higher in MLN lymphocyte of the intraperitoneally-sensitized group. This observation suggests that the route of immunization can affect their immunoreactivity. SPL lymphocytes of the orally-immunized group showed higher proliferation as compared with SPL lymphocytes of the group sensitized before oral immunization. It is likely that the route of antigen administration has induced a specific food tolerance. The results suggest that none of the modifications performed has changed the immunoreactivity of the investigated proteins to a great extent.