Wyniki wyszukiwania - Biblioteka Nauki

1

Zastosowanie techniki real-time PCR w wirusologii

100%

Rynans S. , de Walthoffen S. W. , Dzieciatkowski T. , Mlynarczyk G.

Postępy Mikrobiologii

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2015

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tom 54

|

nr 1

2

Aktualny zakres stosowania pasz GM w żywieniu zwierząt w Polsce na podstawie badań urzędowych

86%

Mazur M. , Sieradzki Z. , Krol B. , Kwiatek K. , Bialowas A. , Puzio H. , Markowski J. , Dobkowicz J. , Kaczmarek J. , Mildner E.

Pasze Przemysłowe

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2018

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tom 27

|

nr 2

3

Możliwości analityczne wykrywania i identyfikacji gatunkowej przetworzonego białka zwierzęcego

86%

Weiner A., Paprocka I., Golebiowska A..,Kwiatek K.

Pasze Przemysłowe

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2017

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tom 26

|

nr 1

4

Zastosowanie metody real-time PCR do wykrywania RNA enterowirusow czlowieka

86%

Les K. , Przybylski M. , Dzieciatkowski T. , Mlynarczyk G.

Medycyna Doświadczalna i Mikrobiologia

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2010

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tom 62

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nr 3

245-253

PL

Celem pracy było zaprojektowanie i optymalizacja warunków reakcji w metodzie real-time PCR z wykorzystaniem sondy typu TaqMan do wykrywania enterowirusów człowieka. Użyto starterów oraz sondy komplementarnych dla konserwatywnych sekwencji w obrębie regionu niekodującego genomu enterowirusów (5'UTR). Sprawdzono swoistość metody wobec 19 taksonów enterowirusów z grup Coxsackie A, Coxsackie B, echowirusów i enterowirusów 68/71, a także innych wirusów zakażających człowieka. Określono zakres czułości metody względem szeregu dziesiętnych rozcieńczeń zawiesiny wzorcowych szczepów enterowirusów.

EN

Infections with human enteroviruses are common worldwide and cause a wide range of signs and symptoms. Nowadays in current diagnostics procedures older virological methods, such virus isolation in a cell cultures and seroneutralisation assay, are replaced with molecular biology tests. The aim of the study was development of real-time PCR assay for detection of human adenoviruses. DNA isolated from MK2 cell line infected with nineteen different enterovirus strains was used for development of a qualitative real-time PCR assay using primers targeting a conserved region of the 5'UTR region and a specific TaqMan® probe. The analytical sensitivity of real-time PCR assay was tested using serial dilutions of Coxackie A9 cDNA in range between 100 and 10-8. For comparison typical end-point detected RT-PCR for enterovirus detection with the same cDNA dilutions was made. The sensitivity of novel method was about ten thousand-fold higher than older one. The conclusion is that real-time PCR is very advisable in diagnostics of diseases caused with enteroviruses. The high level of sensitivity, specificity, accuracy, and rapidity provided by this assay are favorable for the use in the detection of enteroviral RNA in clinical specimens, especially from neuroinfections.

5

Wykrywanie i identyfikacja gatunkowa białek zwierzęcych w surowcach i paszach

86%

Weiner A. , Paprocka I. , Golebiowska A. , Kwiatek K.

Pasze Przemysłowe

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2016

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tom 25

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nr 2

6

Clostridium botulinum w paszach - trudnosci analityczne i występowanie

86%

Grenada T. , Grabczak M. , Kukier E. , Goldsztejn M. , Koziel N. , Kwiatek K.

Pasze Przemysłowe

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2018

|

tom 27

|

nr 2

7

Występowanie przetworzonego białka przeżuwaczy w paszach w latach 2014-2015

86%

Weiner A. , Paprocka I. , Golebiowska A. , Kwiatek K.

Pasze Przemysłowe

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2016

|

tom 25

|

nr 2

8

Nowoczesne metody badania składu gatunkowego mięsa i produktów mięsnych

72%

Wasinski B. , Osek J.

Medycyna Weterynaryjna

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2013

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tom 69

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nr 06

EN

Despite the permanent development of laboratory techniques, various types of adulterations are still a problem in the food industry. An important group among different frauds is adulterations connected with meat species authenticity. Uncovering of adulterated meat products is important inter alia for allergic individuals, for those who can’t intake certain species because of religious beliefs, and for maintaining fair-trade. More subtle techniques used for the mentioned adulterations generate a need for the elaboration of better analytical methods to provide effective control of meat and meat products. The aim of this review is to present currently used laboratory methods applied for meat species identification and detection of adulterations in the declared composition of meat products. The first group of the described methods enables identification and analysis of proteins and the second presented group contains techniques of DNA analysis. Apart from their short characteristics, some disadvantages and potential problems found during work with certain methods are described.

9

Nowoczesne diagnozowanie zanieczyszczenia bakteriami Salmonella

72%

Mięso i Wędliny

|

2005

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nr 7

10

Identyfikacja Francisella tularensis techniką real - time PCR z wykorzystaniem sond hybrydyzujących zaprojektowanych dla fragmentów sekwencji genów fopA i tul4

72%

Bielawska-Drozd A. , Niemcewicz M. , Gawel J. , Bartoszcze M. , Graniak G. , Joniec J. , Kolodziej M.

Medycyna Doświadczalna i Mikrobiologia

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2010

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tom 62

|

nr 4

PL

Badano przydatność zaprojektowanych starterów FopA F/R, Tul4 F/R i sond hybrydyzujących FopA S1/S2, Tul4 S1/S2 dla genów fopA i tul4 do wykrywania F. tularensis. W badaniach, w których użyto 50 szczepów F. tularensis uzyskano wyniki dodatnie. Swoistość reakcji wykazano badając szczepy bakterii non-Francisella tularensis. Przy użyciu starterów FopA F/R i sond hybrydyzujących FopA S1/S2 zaprojektowanych dla genów fopA dodatnie wyniki amplifikacji uzyskano ze wszystkimi badanymi szczepami F. tularensis. Identyczne wyniki otrzymano w reakcji real - time PCR z zastosowaniem starterów Tul4 F/R i sond hybrydyzujących Tul4 S1/ S2 zaprojektowanych dla genu tul4. Swoiste produkty reakcji amplifikacji pojawiły się między 16 a 18 cyklem reakcji. Badania z użyciem starterów i sond hybrydyzujących zaprojektowanych dla genu fopA wykazały, że charakterystyczna temperatura topnienia produktów wynosiła 61°C, a dla genu tul4 60°C. Przy użyciu starterów Tul4 F/R i sond hybrydyzujących Tul4 S1/ S2 czułość oznaczeń wynosiła 10 fg/µl, a przy użyciu starterów FopA F/R i sond hybrydyzujących FopA S1/S2 1 fg/µl.

EN

Tularemia is highly infectious and fatal zoonotic disease caused by Gram negative bacteria Francisella tularensis. The necessity to undergo medical treatment in early phase of illness in humans and possibility of making use of bacterial aerosol by terrorists in an attack create an urgent need to implement a rapid and effective method which enables to identify the agent. In our study two primers FopA F/R and hybridization probes Fop A S1/S2 designed from fop A gene sequence, were tested for their potential applicability to identify F. tularensis. In this research 50 strains of F. tularensis were used and the test gave positive results. Reaction specificity was confirmed by using of non - Francisella tularensis bacterial species. The results obtained in the real-time PCR reaction with primers Tul4 F/R and hybridization probes Tul4 S1/S2, designed from tul4 gene, were comparable to the results from previous experiment with fopA - primers set. Investigation of fopA and tul4 primers and hybridization probes properties revealed characteristic Tm (melting temperature) value of the products - 61 °С and 60°C, respectively. Detection sensitivity was remarkably higher when fopA primers set was used 1 fg/µl, and for tul4 primers set, minimal detectable concentration is 10 fg/µl.

11

Molekularna diagnostyka wybranych patogenów z rodzaju Phytophthora w ramach integrowanej ochrony roślin

72%

Nowakowska J. A. , Malewski T. , Tereba A. , Borys M. , Oszako T.

Sylwan

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2016

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tom 160

|

nr 05

EN

Traditional detection methods such as baiting or direct isolation take a long time and are incapable to handling large volume of material to be tested. The real−time PCR−based techniques are faster, more sensitive, more easily automated, and do not require post−amplification procedures. Species−specific primers for Phytophthora were designed based on the internal transcribed spacer regions (ITS) of rDNA collected from the NCBI DNA database. Primers and probes were designed using the Allele ID 7 at default search criteria. Specific probes were labeled with the reporter dyes JOE (6−carboxy−4,5−dichloro−2,7−dimethoxyfluorescein) at the 5' end and HBQ1 quencher at the 3' end (Sigma−Aldrich). The specificity of primers and fluorogenic probes was tested against genomic DNA of P. alni subsp. multiformis, P. lacustris and P. taxon hungarica. The real−time PCR reactions with the specific probes and primers yielded positive results with five concentrations of standards obtained by standard PCR reaction for corresponding Phytophthora species. The negative control (lack of DNA pathogens) yielded no amplification products. Standard curves showed a linear correlation between input DNA and cycle threshold (Ct) values with R² from 0.994 (P. alni) to 0.998 (P. taxon hungarica). The amplification efficiency of target DNA varied from 94.6% (P. alni) to 100% (P. taxon hungarica). The validation of the primers and probes designed for analysed Phytophthora species was performed on pure cultures, on soil samples from the forest nursery and declining oak stands. The designed probes displayed the high specificity of the detection of investigated species in pure cultures. The presented new molecular TaqMan probes can fully assist the integrated pest management as a powerful tool for a quick detection of above pathogenic organisms in forest nurseries. The molecular detection of harmful phytophthoras and in consequences diminishing of fungicides use for their control in forestry fully support European Union directives as well as the ‘Good plant protection practice measures' elaborated by European and Mediterranean Organisation of Plant Protection.

12

Wykrywanie oraz roznicowanie wirusow opryszczki typu 1 i 2 metoda real-time PCR z wykorzystaniem sond HybProbe

58%

Chudzik E. , Karabin K. , Dzieciatkowski T. , Majewska A. , Przybylski M. , Midak-Siewirska A. , Luczak M. , Mlynarczyk G.

Medycyna Doświadczalna i Mikrobiologia

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2010

|

tom 62

|

nr 3

255-262

PL

Celem pracy była optymalizacja metody real-time PCR do wykrywania oraz różnicowania zakażeń ludzkimi wirusami opryszczki typu 1 i 2, zwłaszcza w przypadkach infekcji przebiegających z niewielkim nasileniem replikacji patogenu.

EN

Herpes simplex viruses types 1 and 2 are members of the Alphaherpesviridae subfamily, as they can infect both skin and nerves and develop latent infection within the dorsal root and trigeminal ganglia. Infections with these viruses are common worldwide and cause wide range of clinical syndromes. Although HSV-1/2 infect healthy children and adults, disease is more severe and extensive in the immunocompromised individuals and/or during neuroinfections. The aim of the study was development of real-time PCR assay for detection and differentiation of herpes simplex viruses type 1 and 2. DNA in clinical samples, using specific dual-channel HybProbe chemistry. The nalytical sensitivity of assay was tested using serial dilutions of HSV-1 and HSV-2 DNA in range between 100 and 10-5 (4,35x105 - 4,00x102 copies/ml and 4,18x105 - 3,82x102 copies/ml, respectively). Thirty four cell line isolates and sixteen clinical samples taken from a group of adult patients with neurological signs were tested for the presence of HSV-1/2 DNA in the LightCycler® instrument. Described in- house real-time PCR assay detected herpesviral DNA in all cell line isolates (31 of them were HSV-1 positive; 3 were HSV-2 positive) and in 10 clinical samples (positive only for HSV-1). The conclusion is that developed HybProbe-based real-time PCR test is very reliable and valuable tool for detection and differentiation of HSV-1/2 viremia in different kind of samples. The high level of sensitivity and accuracy provided by this assay is favorable for the quantification of herpes simplex virus 1 and 2 DNA in clinical specimens, especially during low-copy infections.

13

Molekularne metody wykrywania Listeria monocytogenes w żywności

58%

Lachtara B. , Wieczorek K. , Osek J.

Medycyna Weterynaryjna

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2016

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tom 72

|

nr 01

EN

Listeria monocytogenes is an important foodborne pathogen that causes a disease known as listeriosis, which is especially dangerous for pregnant women. Infection with L. monocytogenes may also result in stillbirths, abortions and premature deliveries, as well as meningitis, septicaemia, encephalitis, and meningoencephalitis. Conventional detection methods of these bacteria are time-consuming; therefore, rapid alternative methods, including those based on molecular tests are needed. PCR is sensitive and specific; however, it requires the use of an agarose gel, which increases the time of analysis. A technique that allows the elimination of this step is real-time PCR, which enables the quantitative determination of L. monocytogenes in foods. A modification of PCR is multiplex PCR that allows detection of several genes at the same time and distinguishes between different species of microorganisms. Techniques such as RT-PCR or NASBA, where the target molecule is RNA, are used to detect viable cells and also allow quantitative analyses to be performed. Another rapid and specific method is LAMP, which can be performed in one hour in a water bath or heating block, without the use of a thermocycler. Biosensors and microarrays are examples of new technologies that due to the possibility to use anywhere and immediate interpretation of the results can be routinely used in the future for identification of L. monocytogenes in food.

14

Monitorowanie chorob wywolanych przez patogeniczne legniowce za pomoca analiz DNA

58%

Nevoigt F. , Oszako T. , Nowakowska J. A.

Sylwan

|

2010

|

tom 154

|

nr 07

450-455

15

Metody badań laboratoryjnych w rozpoznawaniu chorób drobiu

58%

Duszynska-Stolarska O.

Hodowca Drobiu

|

2015

|

nr 04

16

Identyfikacja roznych gatunkow ryb i bezkregowcow morskich technikami biologii molekularnej

58%

Sawicki W. , Daczkowska-Kozon E. , Kwiatkowska B. , Dabrowski W. , Herring H.

Medycyna Weterynaryjna

|

2010

|

tom 66

|

nr 03

182-187

EN

Dietetic and nutritional value of fish and shellfish products have made them very attractive food products for consumers. This results in both the increase of seafood consumption per capita and more diverse offers of finfish/shellfish species on the market. Nevertheless, while pursuing profits some of the producers/importers may replace valuable, more costly raw materials with cheaper substitutes. In order to efficiently monitor what is in the product and not to allow such practices to take place it is necessary to apply reliable, fast methods as to verify their contents. Until recently the methods used to test the products’ components were based mostly on electrophoretic, chromatographic or the immunological tests. At presents to detect food adulteration the PCR technique and its variety based on species polymorphy (multiplex PCR, PCR-RFLP, PCR-RAPD, PCR-AFLP, PCR-FIN or PCR-SSCP) are used. The latest achievements in identification and differentiation between the species are real-time PCR and microarray DNA. Discussions on how to improve the methods of identification/differentiation and on the possible application of such methods in routine quality control of seafood by the appropriate authorities are in progress.

17

Metody identyfikacji gatunkowej grzybów z rodzaju Candida. Część II. Techniki molekularne

58%

Gnat S.

Życie Weterynaryjne

|

2022

|

tom 97

|

nr 03

18

Metody biologii molekularnej wykorzystywane w diagnostyce zakażeń Mycoplasma synoviae u drobiu

58%

Kursa O. , Tomczyk G. , Sawicka A. , Minta Z.

Medycyna Weterynaryjna

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2016

|

tom 72

|

nr 01

EN

Diagnostics of Mycoplasma synoviae (MS) is generally based on serological, culturing and molecular methods. Rapid diagnosis and identification of infections is very important in the poultry industry. The use of PCR or real-time PCR makes it possible to shorten the time for obtaining results from research and the effective detection of genetic material of MS. There are many variations of the PCR method, one of them is the LAMP method, which is rapid, very sensitive and does not require specialist equipment. This article reviews the molecular methods used for the diagnosis of Mycoplasma synoviae infection in poultry.

19

Zastosowanie technik biologii molekularnej w diagnostyce chorób zakaźnych u drobiu

58%

Duszynska-Stolarska O.

Hodowca Drobiu

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2015

|

nr 08

20

Ochratoksyna A w żywności

51%

Ziarno M. , Ujazdowska M.

Przemysł Spożywczy

|

2009

|

tom 63

|

nr 07

PL

Penicillium nordicum to stosunkowo niedawno odkryty gatunek pleśni, mający zdolność do wzrostu w warunkach ekstremalnego zimna, chociaż dość często występujący także w regionach śródziemnomorskich. Występuje głównie w produktach bogatych w tłuszcz i białko, ale może być także izolowany z produktów roślinnych. Istnieje niebezpieczeństwo jego rozwoju w żywności, głównie mrożonej lub przechowywanej w warunkach chłodniczych, suszonej lub z dużą zawartością chlorku sodu, a także w paszach. Najważniejszym wtórnym metabolitem tej pleśni w produktach pochodzenia zwierzęcego i roślinnego oraz paszach są ochratoksyny, głównie ochratoksyna A (OTA). OTA została zakwalifikowana przez IARC do kategorii 2B wśród czynników kancerogennych jako toksyna prawdopodobnie powodująca raka u ludzi.

EN

Penicillium nordicum is relatively recently discovered species of moulds, having the ability to grow in the condition of extreme cold, although occurring also quite often in Mediterranean region. The species occurs mainly in products rich in lipids and proteins as well as in plant environment. There is a possibility of Penicillium nordicum to grow in food, mainly frozen or refrigerated stored, dried or with high salt concentration, as well as in feed. Ochratoxins, mainly OTA, are the most important secondary metabolite of this mould in food and feed. OTA is rated by IARC to 2B class of carcinogenic factors as toxin causing probably the human cancer.