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Model development of hydroxyproline induced hyperoxaluria in young growing pigs

100%

Kateryna Goncharova K. , Rafał Filip R. , Paulina Świeboda P. , Olena Prykhodko O. , Olexandr Fedkiv O. , Katarzyna Szwiec K. , Jose Luis Valverde Piedra J. L. V. , Blanka Majda B. , Stefan Grzegorz Pierzynowski S. G.

nr 1

6–11

Aim of the study. In this study, we sought to create a model of reversible hyperoxaluria in pigs by feeding with hydroxyproline (HP). Materials and methods. The experiment included 12 pigs divided into 2 groups (n = 6). The pigs were fed twice a day. At the beginning of the experiment, in the adaptation period, all pigs were given standard feed. In the next 7 days, an increasing amount of hydroxyproline (1–3% HP), was added to the feed. In next 14 days, 4% HP was administered in each pig meal. After 14 days of 4% HP diet, the pigs were randomly divided into 2 groups. For 6 pigs, 4% HP treatment had been continued for the next 14 days while the second group of pigs for the next 14 days received a standard HP free diet. 24h urine samples, blood and fecal samples were collected on particular days. Results. The addition of HP to the diet increased urinary oxalate excretion. A characteristic increase was noted after 12 days of treatment with 4% HP. During the removal period, oxalate excretion decreased in the group without HP in diet, while in the group which continued with a 4% HP diet, oxalate excretion significantly increased. Gross examination of kidneys showed that in the group which had 4% HP diet for 4 weeks, kidneys were fibrotic with enlarged cavities, and had small visible urinary stones. In second group, kidneys were relatively normal looking with no visible stones. Conclusion. Hyperoxaluria is reversible, if HP is removed 14 days after the start of 4% HP diet. Prolonged exposure up to 4 weeks causes pathologic changes in kidneys including crystals, sand and small stone formation.

Comparison of meat quality characteristics between commercial pigs and snails

63%

Zymantiene J. , Jukna V. , Jukna C. , Zelvyte R. , Oberauskas V.

nr 1

Nowadays traditional pork is still remaining the most popular meat in many European countries. In contrast, presently there is a growing interest in the production and marketing of non traditional snail meat. The aim of the present study was to compare the quality characteristics of pork and snails’ meat, to determine an impact of animal species on meat quality and to analyse the correlation coefficients of some parameters of meat quality. The meat quality characteristics, as chemical composition and some physical properties, were evaluated after deep-freezing. It was determined that snail meat contained by 11.50% higher amount of water, by 14.60% higher amount of mineral substances and by 77.04% higher amount of amino acid hydroxyproline, but by 77.38% less fat, by 37.94% less protein and by 43.28% tryptophan if to compare to the pork meat. Besides, pH in snail meat tended to be alkaline while in pork meat – acid. Moreover, snail meat was darker, pale red and its yellowness was more intensive than that of the pork meat. The correlation between fat and protein in pork and snail meat, pH with tryptophan and hydroxyproline was negative, whereas pH with fat, and dry matter with ash – positive. However, in pork meat tryptophan and hydroxyproline correlated negatively, while in snail meat – positively. Meat chemical composition and physical properties were effected by animal species.

Rice callus growth, proline content and activity of proline and IAA oxidase under the influence of hydroxyproline and NaCl

63%

Chauhan V. A. , Prathapasenan G.

nr 2

Calli of salt tolerant (Bhoora rata) and salt susceptible (GR₁₁) rice varieties were cultured on Linsmaeir and Skoog’s medium containing LD₅₀ concentration of NaCl (200 mM) and hydroxyproline (10 mM). Growth, proline content and activity of proline and IAA oxidases of the cultured tissues were determined at the end of 0, 2, 4, and 6 weeks of incubation. Hydroxyproline resistant calli of both rice varieties when cultured on Linsmaeir and Skoog’s medium containing hydroxyproline and NaCl showed increased dry weight and proline content as compared to NaCl stressed calli. The levels of proline and IAA oxidases were also low in the hydroxyproline resistant calli.

Metody chemicznej kontroli jakosci produktow miesnych

32%

Klossowska B.

tom 05

nr 4

5-17