Wyniki wyszukiwania - Biblioteka Nauki

1

Wybrane metody molekularne wykorzystywane w ocenie bioróżnorodności mikroorganizmów glebowych

100%

Lyszcz M. , Galazka A.

Postępy Mikrobiologii

|

2016

|

tom 55

|

nr 3

2

Techniki analizy restrykcyjnej, hybrydyzacji i klonowania kwasów nukleinowych

100%

Kasprzak W.

Wiadomości Parazytologiczne

|

1988

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tom 34

|

nr 4-6

3

Zastosowanie metod biologii molekularnej w diagnostyce bakteryjnych chorob roslin

100%

Pulawska J. , Sobiczewski P.

Postępy Nauk Rolniczych

|

1997

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tom 44

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nr 1

73-96

EN

Recent years have seen a great increase of interest in application of molecular biology methods (hybridization of nucleic acids, polymerase chain reaction and ligase chain reaction) in the diagnostics of plant bacterial diseases. These methods are incomparably more sensitive than the traditional and the serological ones, thus making possible detection and correct identification of even low number of bacteria and, what 18 most important, results are obtainable in a significantly shorter time - even in a few hours. The paper reviews the most recent literature concerning applications of molecular methods to detection of bacteria belonging to genus: Agrobacterium, Clavibacter, Erwinia, Pseudomonas and Xanthomonas. A serious disadvantage of these methods is their high cost resulting from the need for expensive laboratory equipment and chemicals. Another drawback is the use of hazardous materials such as ethidium bromide (PCR) and radioactive isotopes (hybridization, LCR). Nevertheless, the molecular methods are more and more commonly used in phytopathological laboratories, especially in those examining quarantine objects. They are particularly useful as an assisting tool when results of traditional and serological tests are equivocal.

4

Hybrydyzacja kwasow nukleinowych - zastosowanie w inzynierii genetycznej i diagnostyce

84%

Reichert M. , Grundboeck J.

Medycyna Weterynaryjna

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1993

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tom 49

|

nr 06

256-260

5

Zastosowanie metod biologii molekularnej w diagnostyce weterynaryjnej

84%

Winiarczyk S.

Medycyna Weterynaryjna

|

1993

|

tom 49

|

nr 06

260-264

6

Zastosowanie metod detekcji kwasow nukleinowych do diagnostyki oraz roznicowania zakazen koronawirusowych [TGEV i PRCV] u swin

67%

Gradzki Z.

Rozprawy Naukowe. Akademia Rolnicza w Lublinie

|

1998

|

tom 210

1-98

7

Zastosowanie sond cDNA do wykrywania i roznicowania RNA wirusa TGEV

67%

Gradzki Z.

Medycyna Weterynaryjna

|

2001

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tom 57

|

nr 08

598-602

8

Metody stosowane w diagnostyce mastitis. Cz. III. Molekularne techniki identyfikacji drobnoustrojow

67%

Dudko P.

Magazyn Weterynaryjny

|

2004

|

tom 13

|

nr 12

36-39

9

Listeria monocytogenes w żywności - metody wykrywania i oznaczania

67%

Nowak A. , Oltuszak-Walczak E.

Przemysł Spożywczy

|

2012

|

tom 66

|

nr 11

PL

Bakterie Listeria monocytogenes są czynnikiem etiologicznym wielu zakażeń i zatruć pokarmowych. Najczęściej jest nimi zanieczyszczone mięso, drób, ryby i produkty mleczne. Referencyjne metody wykrywania i oznaczania liczby L. Monocytogenes są praco- i czasochłonne. W artykule omówiono modyfikacje referencyjnych metod wykrywania L. monocytogenes i metody alternatywne, dzięki którym jest możliwe skrócenie czasu detekcji tych patogennych bakterii.

EN

Listeria monocytogenes is well known dangerous food-borne pathogen. Meat, poultry, fish and dairy products are most frequently contaminated with Listeria. Analytical reference methods for detection and enumeration of Listeria monocytogenes in food samples are tedious long lasting. Paper describes the modified and alternative methods allowing for shortening of detection time of this important pathogen.

10

Postępy w diagnostyce serologicznej parazytoz

67%

Dzbenski T. H.

Wiadomości Parazytologiczne

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1988

|

tom 34

|

nr 4-6

EN

Recent advances in the serological diagnosis of parasitic infections were discussed with particular reference to the immunoenzymatic methods of the diagnosis, antigen production by recombinant gene technology, and nucleic acid hybridization.

11

Metody roznicowania gatunkow nicieni z rodzaju Trichinella

67%

Pastusiak K.

Wiadomości Parazytologiczne

|

2006

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tom 52

|

nr 3

165-173

EN

This review summarizes the major biological, biochemical and molecular methods which have been developed during last 20 years to distinguish parasites of the genus Trichinella. From the time of the discovery of Trichinella in 1835 until the 1970, it was assumed that trichinellosis was caused by a single species of parasite, Trichinella spiralis. Many biological parameters have been compared to differentiate the parasite, such as host specificity, geographical distribution, reproductive abilities, nurse cell development and resistance to freezing. Now, investigators realize that the genus Trichinella is a much more complex group of parasites and simple biological methods are unsufficient. In order to identify and better characterize the species and genotypes of Trichinella it was necessary to develop more sensitive techniques. First, for detecting Trichinella infection immunological methods have been used, such as detection of antibodies in host blood and antigens of parasites using monoclonal antibodies against immunodominant proteins. Later, biochemical techniques have been used such as isoenzyme analysis. The main goal of these methods is to provide a simple, rapid and reproducible techniques to differentiate Trichinella parasites. For this purpose DNA-based methods appeared the best ones. Beginning with the use restriction enzymes, repetitive DNA probes for detection of parasite DNA, and later techniques based on the polymerase chain reaction (PCR), give results at the high level of sensitivity. All of this information has been used to construct a new taxonomy of the genus Thrichinella. To date, 11 taxa have been recognized in the genus: 8 species (Trichinella spiralis T1, Trichinella nativa T2, Trichinella britovi T3, Trichinella pseudospiralis T4, Trichinella murrelli T5, Trichinella nelsoni T7, Trichinella papuae T10, Trichinella zimbabwensis T11) and additionally three genotypes whose taxonomic status is yet uncertain (T6, T8, T9). Based upon morphology, epidemiology of trichinellosis, geographical distribution and host range of the parasite, two main groups are recognized in the genus Trichinella. The first group comprises species that encapsulate in host muscle tissue, while the species of the second group do not encapsulate. The species and genotypes of the first group infect only mammals (T. spiralis, T. nativa, T. britovi, T. murrelli, T. nelsoni, T6, T8 and T9), whereas of the three species from the second group, one parasitises mammals and birds (T. pseudospiralis) and the other two infect mammals and reptiles (T. papuae and T. zimbabwensis). Due to the big genetic differences between Trichinella isolates, investigators predict that the number of species and genotypes found within Trichinella will be increased.

12

Wspolczesne metody diagnostyki laboratoryjnej chorob zakaznych

59%

Klimuszko D. , Binek M.

Życie Weterynaryjne

|

2006

|

tom 81

|

nr 10

666-671