Wyniki wyszukiwania - Biblioteka Nauki

1

Quantification of taxol by high-performance thin layer chromatography in Taxus wallichiana callus cultivated in vitro

100%

Mujib A. , Tonk D. , Gulzar B. , Maqsood M. , Ali M.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2020

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tom 101

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nr 4

2

Rapid high performance thin layer chromatographic method for quantitation of catechin from extracts of cashew leaves - a short report

100%

Jaiswal Y. , Tatke P. , Gabhe S. , Vaidya A.

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tom 63

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nr 1

EN

Cashew is a tropical tree with immense commercial value and the use of its various parts in the confectionary, food, herbal and tanning industry has gained tremendous importance. Thus, the development of quality control method for routine analysis of various products of this widely used plant would be a great help to analysts. The phytochemistry of this plant has not been explored in detail. Anacardium occidentale Linn. (cashew) is reported to contain many antioxidant and polyphenolic compounds. However, there have been no reports published for the HPTLC analysis of the extracts of cashew leaves. Hence, an attempt has been made to develop a HPTLC method for estimation of catechin from leaf extracts. The present paper reveals a rapid high performance thin layer chromatographic method developed for quantitation of catechin from extracts of cashew leaves. The chromatographic parameters such as solvent system, development time, saturation time, detection wavelength were optimized. The mobile phase toluene: ethyl acetate: methanol: formic acid (6:6:1:0.1v/v/v/v) gave the best resolution for various components. The separation of various components and quantitation of amount of catechin was successfully carried out from extracts of cashew leaves. The aqueous extract of leaves contained a higher amount of catechin as compared to ethanol extract. The presence of tannins and phenolics was visualised as bluish-black bands with 5% alcoholic FeCl3 as visualising agent. The method can prove to be a rapid, sensitive and economic alternative as compared to other chromatographic methods especially HPLC for detection of catechin in various food products and herbal formulations.

3

Lysophosphatidylcholines: bioactive lipids generated during storage of blood components

100%

Maslanka K. , Smolenska-Sym G. , Michur H. , Wrobel A. , Lachert E. , Brojer E.

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nr 1

4

Investigations of phenolic acids occuring in plant components of Naran N by HPLC and HPTLC-densitometric methods

100%

Wojciak-Kosior M. , Matysik G. , Soczewinski E.

Herba Polonica

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2003

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tom 49

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nr 3-4

EN

The content of phenolic acids was determined by high performance thin-layer chromatography and densitometry and by HPLC in herbal preparation Naran N elaborated in the Department of Chemistry of the Medical University in Lublin, Poland. Methanolic extracts from plant components of Naran N: Anthodium Chamomillae, Herba Euphrasiae, Folium Plantaginis lanceolatae, Flos Calendulae were analysed. In the extract from Anthodium Chamomillae protocatechuic, vanillic, ferulic, caffeic, chlorogenic and p-coumaric acids were identified; in the extract from Herba Euphrasiae p-coumaric, ferulic, caffeic, protocatechuic, vanillic, chlorogenic and 3-hydroxybenzoic acids, in the methanolic extract from Folium Plantaginis lanceolatae vanillic, p-coumaric, caffeic, ferulic, chlorogenic and protocatechuic acids were found; the same number of phenolic acids were detected in the methanolic extract of Flos Calendulae, these were p-coumaric, caffeic, syringic, chlorogenic, vanillic, protocatechuic and ferulic acids.

PL

Przy użyciu metod chromatograficznych HPTLC w połączeniu z densytometrią i HPLC badano kwasy fenolowe w kompozycji ziołowej Naran N opracowanej w AM w Lublinie. Badano ekstrakty metanolowe z surowców wchodzących w skład Naranu N: Anthodium Clm- momillae, Herba Euphrasiae, Folium Plantciginis lanceolatae, Flos Calendulae. W ekstrakcie z Anthodium Chamomillae zidentyfikowano kwas protokatechowy, wanilinowy, ferulowy, kawowy, chlorogenowy i p-kumarowy. W ekstrakcie metanolowym z Herba Euphrasiae zidentyfikowano kwasy: p-kumarowy, ferulowy, kawowy, protokatechowy, wanilinowy, chlorogenowy i 3-hydroksybenzoesowy. W ekstrakcie metanolowym z Folium Plantaginis lanceolatae stwierdzono obecność kwasów: wanilinowego, p-kumarowego, kawowego, ferulowego, chlorogenowego i protokatechowego. Taką samą liczbę kwasów fenolowych zidentyfikowano w ekstrakcie metanolowym z Flos Calendulae. Były to kwasy: p-ku- marowy, kawowy, syryngowy, chlorogenowy, wanilinowy, protokatechowy i ferulowy.

5

Combined SPE and HPTLC as a screening assay of urinary cotinine from male adolescents exposed to environmental tobacco smoke

100%

Bazylak G. , Brozik H. , Sabanty W.

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nr 2

EN

Cotinine, as the main metabolite of nicotine, has been determined in urine using solid-phase extraction and the high-performance thin-layer chromatographic (SPE-HPTLC) method. The urine samples were collected from a group of 35 male adolescents which were moderate or significantly exposed to home environmental tobacco smoke (ETS). l-methyl-2-pyrrolidinone was used as the internal standard in the proposed screening procedure. The thin-layer chromatograms were evaluated densitometrically after visualization of cotinine spots with ninhydrin and cadmium acetate solution. The described SPE-HPTLC procedure indicated good selectivity, sensitivity and reproducibility, enabling reliable verification of interview collected questionnaire data in families exhibiting a diversified level of ETS. The results of cotinine measurements by the proposed method were applied for assessment of hazards from home ETS on the health status of elementary schoolboys, especially an increased risk for infectious respiratory tract diseases and exercise-induced bronchospasm.