Wyniki wyszukiwania - Biblioteka Nauki

1

Influence of some acyclic aminephosphonates on hemolysis of red cells and stability of planar lipid membranes

100%

Dziamska A. , Sarapuk J. , Kleszczyńska H.

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tom Vol. 48, nr 22

189-192

EN

The interaction of some derivatives of aminemethane phosphonic acid with erythrocytes (RBC) and planar lipid membranes (BLM) was studied. Aminephosphonates were synthesized to be used as pesticides and differed in their polarity and hydrophobicity of the substituents on nitrogen and phosphorus atoms. Their structural features should enable them to interact with the lipid phase of biological objects. That was the reason to make introductory evaluation of their potential biological activity by studying on their interaction with mentioned models. We investigated the influence of aminephosphonates on stability of BLM and hemolysis of RBC. The measured parameters were so-called critical concentration (CC) of the compounds causing destruction of BLM in arbitrarily chosen time of 3 min, and causing 100% hemolysis of RBC (C100). Additionally the fluorescence spectroscopy method was used to follow changes in fluidity of erythrocyte ghosts cased by the compounds studied. The measured parameter was the anisotropy coefficient (A). It was found that all the compounds studied exhibited good enough efficiency to destroy model membrane studied to classify them as potentially good pesticides. The observed differences in the interaction with membranes used may be attributed to structural differences between the aminephosphonates and the resulting differences in lipophilicity.

PL

Zbadano oddziaływanie nowo zsyntetyzowanych związków fosforoorganicznych z błonami erytrocytów i płaskimi błonami lipidowymi. Badane aminofosfoniany były zróżnicowane hydrofobowo Celem pracy było znalezienie wpływu tego zróżnicowania na efektywność oddziaływania związków z błonami modelowymi. Wybór tych ostatnich uzasadniają wcześniejsze badania, w których wykazano ich przydatność do wstępnego, szybkiego testowania związków syntetyzowanych z myślą o ich biologicznym zastosowaniu. Badanymi parametrami były stężenia aminofosfonianów powodujących całkowitą dezintegrację błon modelowych tzn. 100% hemolizę erytrocytów lub niszczenie błon lipidowych w określonym arbitralnie czasie. Ponadto, celem -wyjaśnienia mechanizmu oddziaływania aminofosfonianów na błony modelowe, wykonano metodą spektroskopii fluorescencyjnej pomiary zmian ciekłości błon cieni erytrocytów przy pomocy dwu sond fluorescencyjnych zlokalizowanych na różnych głębokościach fazy lipidowej błony. Otrzymane wyniki wskazują, że badaną grupę aminofosfonianów można zaliczyć do pestycydów o potencjalnie dobrej skuteczności biologicznej.

2

Hemolytic and physiological activity of new synthesized aminephosphonates

86%

Dziamska A. , Sarapuk J. , Kleszczyńska H. , Bielecki K.

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tom Vol. 48, nr 22

193-196

EN

Some new, synthesized cyclic and acyclic aminephosphonates were studied. Two model membranes were used in order to determine potential biological activity of those compounds. They were erythrocytes (RBC) and planar lipid membranes (BLM). The choice of the model membranes was justified by earlier studies on determination of potential biological usefulness of several quaternary ammonium salts. It was found that the -models used were very convenient tools for preliminary estimation of this type of compounds, which because of their hydrophobicity readily interact with lipid model membranes or the lipid phase of biological membranes. Moreover, a very high qualitative agreement was found between the results of model and biological experiments. Measurements performed permitted the determination of concentrations of the compounds musing total destruction of RBC (100% hemolysis - C100) or BLM in predetermined time (3 min - CC). The -results obtained was then compared with the results of biological tests on the aqueous plant Spirodela oligorhiza. The parameter measured in physiological tests was concentration of the compounds causing 50% inhibition of plant growth (IC50). All experiments were also performed for two herbicides known for their physiological activities. Those were dibutyl ester of 9-(N-n-butylamine)-phosphonic acid-1 (BAPA) and diethyl ester of 9-(N-n-butylamine)fluorenephosphonic acid-9. It was found that acyclic compounds exhibited good enough efficiency to destroy model membrane studied to classify them as potentially good pesticides. The efficiency of cyclic compounds was significantly worse. The observed differences between efficacies of the compounds toward both plant and model membrane is discussed.

PL

Zbadano oddziaływanie nowo zsyntetyzowanych aminofosfonianów na błony erytrocytów, płaskie błony lipidowe i na roślinę wodną Spirodela oligorrhiza. Badane aminofosfoniany były zróżnicowane strukturalnie. Celem pracy było znalezienie wpływu tego zróżnicowania na efektywność oddziaływania związków, jego mechanizm określenie ich przydatności biologicznej. Badanymi parametrami były stężenia aminofosfonianów powodujących całkowitą dezintegrację błon modelowych oraz inhibicję wzrostu Spirodeli oligorrhizy. Otrzymane wyniki wskazują, te badaną grupę aminofosfonianów można zaliczyć do pestycydów o potencjalnie dobrej skuteczności biologicznej.

3

Determination and haemolytic activity of saponins in hairy root culture of Platycodon grandiflorum A.DC.

86%

Urbanska N. , Nartowska J. , Skorupska A. , Ruszkowski D. , Giebultowicz J. , Olszowska O.

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2009

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tom 55

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nr 3

103-108

EN

The sum of saponins in the hairy root lines (6, 17) of Platycodon grandiforum A.DC. was compared. Hairy root line 6 showed a higher total saponin content (6.92%) than the line 17 (6.01%). According to the Chinese Pharmacopoeia standards the content of saponins in Platycodi Radix should be not less than 2%. Our results seem to indicate that the hairy root culture of Platycodon grandiforum A.DC. is a good source of saponins. The Haemolytic Index of the hairy root line 6 was 1600. Digitonin was used as a reference. Moreover, the haemolytic activity of TLC subfractions of saponins varied.

PL

Porównano zawartość sumy saponin w dwóch klonach 6 i 17 korzeni transformowanych rozwaru wielkokwiatowego (Platycodon grandiforum A.DC.). Klon 6 zawierał więcej (6,92%) saponin niż klon 17 (6,01%). Suma saponin w surowcu zgodnie z wymaganiami Farmakopei Chińskiej nie powinna być mniejsza niż 2%. Na podstawie uzyskanych rezultatów można stwierdzić, że kultura in vitro korzeni włośnikowatych Platycodon grandiforum A.DC. jest wydajnym źródłem saponin. Dla klonu 6 określono również wskaźnik hemolityczny. Wynosił on 1600 względem digitoniny jako saponiny wzorcowej. Wykazano również różną siłę hemolityczną poszczególnych podfrakcji saponin rozdzielonych metodą TLC.

4

The physicochemical properties of some new aminophosphonates

86%

Kleszczynska H. , Sarapuk J. , Dziamska A.

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2000

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tom 05

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nr 4

415-422

EN

The hemolytic activity of some newly synthesized aminomethanephosphonic acid derivatives was studied. The compounds studied differed in their polarity and the hydrophobicity of the electronic substituents at their nitrogen and carbon atoms. It was found that acyclic aminophosphonates exhibited significantly stronger hemolytic properties than cyclic aminophosphonates. To cause the same level of hemolysis of pig erythrocytes, it was necessary to use about a tenfold higher concentration of cyclic aminophosphonates than acyclic ones. The hemolytic activities of the compounds were related to the possibilities of their incorporation into the lipid phase of erythrocyte membranes. Once incorporated, they changed the fluidity of those membranes; the changes were more pronounced in the case of cyclic aminophosphonates. Acyclic compounds were also found to exhibit a slight antioxidative activity, which may be a consequence of their stronger interaction with the membrane lipids. The results obtained in the experiments performed with the use of planar lipid membranes were similar to those obtained in the hemolytic studies, i.e., acyclic aminophosphonates interacted more effectively with those membranes. The general conclusion is that both stereochemistry and hydrophobicity are the factors that determine the efficiency of the interaction of the compounds studied with the model membranes used, and that most likely location of aminophosphonates is the lipid phase of the erythrocyte membrane. Another conclusion is that newly synthesized aminophosphonates may be used as potentially good pesticides.

5

Haemolytic and proteolytic activity of coagulase-negative staphylococci isolated from mastitis cows

86%

Bochniarz M. , Wawron W.

Polish Journal of Veterinary Sciences

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2012

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tom 15

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nr 1

EN

The aim of the present study was to assess the haemolytic and proteolytic activity of coagulase- negative staphylococci (CNS) isolated from cows with mastitis. The study was conducted on 100 CNS strains: S. xylosus (n=28), S.chromogenes (n=26), S.haemolyticus (n=25), S. sciuri (n=14), S. warneri (n=4), S.hominis (n=2), S.saprophyticus (n=1); 22 CNS were isolated from cows with clinical mastitis and 78 from those with subclinical mastitis. The CNS studied showed the ability to produce only α-haemolysin and belonged to one strain – S. haemolyticus (21.0% of isolated CNS strains). Haemolysin-positive CNS were responsible for both clinical and subclinical mastitis (22.7% and 20.5%, respectively). The ability to produce protease was found in 31.0% of CNS belonging to two strains: S. chromogenes and S. sciuri. Protease-positive CNS were the etiological factor of both clinical and subclinical mastitis (31.8% and 30.8%, respectively). All S. xylosus, S. warneri, S. hominis, and S. saprophyticus strains were found protease-negative and haemolysin-negative, irrespective of whether they caused clinical or subclinical mastitis in cows.

6

Hemolytic activity of fractions of the ethanolic extract from faba bean cotyledons

86%

Karamac M. , Kosinska A. , Shimoyamada M. , Amarowicz R.

Polish Journal of Natural Sciences. Supplement

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2006

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tom 03

79-85

7

The in vitro hemolytic activity of erythrocytes from free-living mute swan (Cygnus olor) spread out in Gdynia and Sopot Coasts

86%

Tkachenko H. , Kurhaluk N. , Hetmanski T.

Baltic Coastal Zone. Journal of Ecology and Protection of the Coastline

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2018

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tom 22

EN

The erythrocytes could be considered as a valuable model in studying the cytotoxic effect of environmental xenobiotics owing to its particular structure and the important components of their plasma membrane especially lipids and proteins. The aim of our study was to investigate the erythrocyte hemolytic activity of mute swan (Cygnus olor) from Baltic coastal region in two points, i.e. Sopot and Gdynia. These comparisons will show whether mute swans on the Baltic coastal regions have elevated levels of hemolysis plus gender variation in erythrocyte damage. Forty adult mute swans were captured from Sopot and Gdynia in February 2013. Collected birds were measured and weighted, and the blood was sampled. The birds were divided into 4 groups (after the 2nd/3rd year of life): males from Sopot (n = 10), females birds from Sopot (n = 10), males from Gdynia (n = 10), and females from Gdynia (n = 10). Blood samples were collected from the medial metatarsal vein in sodium citrate for erythrocyte sedimentation rate. Blood was stored in tubes and held on the ice until centrifugation. The plasma was removed. The erythrocyte suspension (one volume) was washed with five volumes of saline solution three times. The acid resistance of erythrocytes was induced by 0.1M HCl, while osmotic resistance was induced by incubation erythrocyte suspension with the different solution of urea and NaCl, as well as in solutions of different NaCl concentration (0.1-0.9%). The erythrocyte hemolysis was measured spectrophotometrically. Our study revealed that the highest level of the hemolysis percentage was observed among females from Gdynia, males from Sopot, then females from Sopot, and males from Gdynia. The significant differences between the hemolysis percentage among females from Gdynia and Sopot from 13.5 to 18.0 min after HCl-induced hemolysis was observed. The results also indicated significant differences between the hemolysis percentage among males from Gdynia and Sopot from 14.0 to 19.0 min. Hemolysis percentage showed to be increased not significantly among males from Gdynia with time from hemolysis initiation to 10.0 min but close to linearity with a value of hemolysis percentage among males from Sopot after 25 min of hemolysis initiation. The maximal level of hemolysis percentage was significantly higher among females from Gdynia (at 15.0 min) compared to females from Sopot (at 14.0 min). The maximal level of hemolysis percentage among males from Gdynia and Sopot was noted at 0.5 min after hemolysis initiation. The maximal level of hemolysis percentage was 4.40% and 4.11%, respectively. The highest percentage of hemolyzed erythrocytes after incubation with solutions of various urea concentration was observed both among males and females from Gdynia. The lowest percentage of hemolyzed erythrocytes was exhibited by the erythrocyte suspension of males from Sopot. Comparing the percentage of hemolyzed erythrocytes both among males and females from Sopot, the higher values of hemolyzed erythrocytes was noted among females. The highest percentage of hemolyzed erythrocytes after incubation with solutions of various NaCl concentration was observed among males and females from Sopot and further among males and females from Gdynia after incubation with 0.5-0.9% NaCl solution. Comparing the percentage of hemolyzed erythrocytes after incubition with 0.1-0.4% NaCl solution, the highest percentage of hemolyzed erythrocytes was observed among males and females from Gdynia and then among males and females from Sopot. Our study showed more individual variation, probably related to the impact of the environmental habitat and consumption of ingested prey items by mute swans. Of those males and females, females from Gdynia showed the highest level of hemolyzed erythrocytes.

PL

Erytrocyty można uznać za cenny model do badania cytotoksycznego działania środowiskowych ksenobiotyków ze względu na ich szczególną strukturę i ważne składniki ich błony komórkowej, zwłaszcza lipidy i białka. Celem naszych badań była ocena aktywności hemolitycznej erytrocytów łabędzia niemego (Cygnus olor) z regionu morza Bałtyckiego (plaże w Sopocie i Gdyni). Porównania te miały pokazać, czy populacja łabędzia niemego w nadbałtyckich regionach przybrzeżnych ma podwyższoną hemolizę erytrocytową oraz czy te zmiany zależą od płci ptaków. Czterdzieści dorosłych łabędzi niemych przebadano w Sopocie i Gdyni w lutym 2013 r. Ptaki podzielono na 4 grupy (po II/III roku życia): samce z Sopotu (n = 10), samice z Sopotu (n = 10), samce z Gdyni (n = 10) i samice z Gdyni (n = 10). Próbki krwi pobierano z przyśrodkowej żyły skoku w probówki z cytrynianem sodu. Krew przechowywano w probówkach i trzymano na lodzie aż do odwirowania. Osocze zostało usunięte. Zawiesinę erytrocytów (jedna objętość) przemyto pięcioma objętościami roztworu soli fizjologicznej trzykrotnie. Kwasową odporność erytrocytów indukowano 0,1M HCl, podczas gdy odporność osmotyczną indukowano przez inkubację zawiesiny erytrocytów w różnych roztworach mocznika i NaCl, a także w roztworach o różnym stężeniu NaCl (0,1-0,9%). Hemolizę erytrocytów mierzono spektrofotometrycznie. Najwyższy poziom hemolizy zanotowano u samic z Gdyni, samców z Sopotu, następnie samic z Sopotu i samców z Gdyni. Stwierdzono istotne różnice między odsetkiem hemolizy u samic z Gdyni i Sopotu z 13,5 do 18,0 min po hemolizie indukowanej HCl. Wyniki wskazują również na istotne różnice pomiędzy odsetkiem hemolizy wśród samców z Gdyni i Sopotu od 14,0 do 19,0 min indukowanej hemolizy. Procent hemolizy zwiększył się nieznacznie u samców z Gdyni w czasie od rozpoczęcia hemolizy do 10 min. Maksymalny odsetek hemolizy był istotnie wyższy wśród samic z Gdyni (po 15,0 min) w porównaniu z samicami z Sopotu (14,0 min). Maksymalny poziom hemolizy wśród samców z Gdyni i Sopotu zanotowano w 0,5 minuty po inicjacji hemolizy. Maksymalny poziom hemolizy wynosił odpowiednio 4,40% i 4,11%. Najwyższy odsetek hemolizowanych erytrocytów po inkubacji z roztworami o różnym stężeniu mocznika obserwowano zarówno u samców, jak i u samic z Gdyni. Najmniejszy odsetek hemolizowanych erytrocytów wykazywała zawiesina erytrocytów samców z Sopotu. Porównując odsetek hemolizowanych erytrocytów zarówno u samców, jak i samic z Sopotu, wyższe wartości hemolizowanych erytrocytów odnotowano wśród samic. Najwyższy odsetek hemolizowanych erytrocytów po inkubacji z roztworami o różnym stężeniu NaCl zaobserwowano u samców i samic z Sopotu, a następnie u samców i samic z Gdyni po inkubacji z 0,5-0,9% roztworem NaCl. Porównując odsetek hemolizowanych erytrocytów po inkubacji z 0,1-0,4% roztworem NaCl, najwyższy odsetek hemolizowanych erytrocytów zaobserwowano u samców i samic z Gdyni, a następnie u samców i samic z Sopotu. Nasze badanie wykazuje większą zmienność osobniczą, prawdopodobnie związaną z oddziaływaniem środowiska naturalnego i spożyciem połkniętych przedmiotów przez nieme łabędzie na plażach Trójmiasta. Spośród samic i samców łabędzi niemych samice z Gdyni wykazały najwyższy poziom hemolitycznych erytrocytów.

8

Gemini ester quat surfactants and their biological activity

72%

Luczynski J. , Frackowiak R. , Wloch A. , Kleszczynska H. , Witek S.

Cellular and Molecular Biology Letters

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2013

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tom 18

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nr 1

EN

Cationic gemini surfactants are an important class of surface-active compounds that exhibit much higher surface activity than their monomeric counterparts. This type of compound architecture lends itself to the compound being easily adsorbed at interfaces and interacting with the cellular membranes of microorganisms. Conventional cationic surfactants have high chemical stability but poor chemical and biological degradability. One of the main approaches to the design of readily biodegradable and environmentally friendly surfactants involves inserting a bond with limited stability into the surfactant molecule to give a cleavable surfactant. The best-known example of such a compound is the family of ester quats, which are cationic surfactants with a labile ester bond inserted into the molecule. As part of this study, a series of gemini ester quat surfactants were synthesized and assayed for their biological activity. Their hemolytic activity and changes in the fluidity and packing order of the lipid polar heads were used as the measures of their biological activity. A clear correlation between the hemolytic activity of the tested compounds and their alkyl chain length was established. It was found that the compounds with a long hydrocarbon chain showed higher activity. Moreover, the compounds with greater spacing between their alkyl chains were more active. This proves that they incorporate more easily into the lipid bilayer of the erythrocyte membrane and affect its properties to a greater extent. A better understanding of the process of cell lysis by surfactants and of their biological activity may assist in developing surfactants with enhanced selectivity and in widening their range of application.

9

Changes in erythrocyte membrane permeability induced by verapamil, chlorpromazine, and their combinations with amphotericin B

72%

Knopik-Skrocka A. , Bielawski J. , Chowanski S. , Walkowiak P.

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tom 48

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nr 2

10

Hemolysis and antioxidative protection of erythrocytes by functionalized quaternary ammonium salts

72%

Kleszczynska H. , Bonarska D. , Oswiecimska M. , Sarapuk J.

Polish Journal of Environmental Studies

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2003

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tom 12

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nr 1

EN

The results of studies on hemolytic and antioxidative activities of quaternary ammonium bromides and chlorides differing in alkyl chain length and with functionalized antioxidative group are presented. Pig erythrocytes (RBC) and their ghosts were used in experiments. The hemolytic studies permitted to determine the safe concentrations at which the compounds studied did not damage RBC membranes. RBC were then UV-irradiated and the antioxidative efficiency of the quats determined. It was found that hemolytic activities increased with lipophilicity of a compound and each bromide salt was more hemolytic than chloride ones. The antioxidant activity of the quats followed the same rule. The results obtained indicate that observed effects are the consequence of the incorporation of particular compounds to different depths into the lipid phase of the RBC membrane. The incorporation depended on lipophilicity of quats and the kind of counterions. Studies on fluidity changes induced by quats in ghost membranes confirmed the above conclusion.

11

Assessment of biological activity of new compounds designated to act as lysosomotropes

72%

Kleszczynska H. , Bonarska D. , Luczynski J. , Witek S. , Sarapuk J.

Polish Journal of Environmental Studies

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2005

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tom 14

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nr 6

EN

This work contains the results of studies on the influence of new lysosomotropic substances on an erythrocyte membrane. The compounds studied were hydrochlorides of N,N-dimethylglycine alkyl esters (DMG-n) and N,N-dimethylalanine alkyl esters (DMAL-n) having two different-length alkyl chains (n = 12 and 16), oxalates of dimethylaminoalaninates (DMALs -n; n = 8, 10, 12, 14 and 16) and methobromides of glycinates and alaninates (DMALM-12 and DMGM-12). They were found to hemolyze erythrocytes, to change their osmotic resistance and to influence erythrocyte membrane fluidity. The results obtained indicate that observed changes were dependent on lipophilicities of the compounds. It was especially evident in the case of hemolytic efficiencies of the homologous series of alanine oxalates. Also, DMG-n and DMAL-n compounds significantly differed in their hemolytic properties. Again, slightly better hemolytic efficiency of DMG compounds in comparison with corresponding compounds having the same alkyl chain, DMAL, confirm such a conclusion. However, their hemolytic efficiencies were found to be moderate, which makes them potentially useful membrane modifiers. That feature is important for lysosomotropic compounds and its confirmation was the primary aim of the presented work. It is worth mentioning that DMGM and DMALM compounds exhibited better hemolytic efficiencies than all other compounds studied – which is probably caused by the fact that they were used as bromides. Bromides are commonly found to be more active than compounds with other counterions.

12

Comparison of acute haematotoxicity of 2-methoxyethanol, 2-ethoxyethanol and 2-butoxyethanol in male rats

72%

Starek A. , Szabla J. , Szymczak W. , Zapor L.

Acta Toxicologica

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2006

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tom 14

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nr 1-2

13

Modifications of hemolytic activity of polyene antibiotics by mono- and disaccharides in mammalian erythrocytes

58%

Knopik-Skrocka A. , Bielawski J. , Wrzeszcz K. , Bochanysz A. , Nowak K.

Biological Letters

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2007

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tom 44

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nr 1

17-30

14

Association of listeriolysin O with the cell surface of Listeria monocytogenes

58%

Bielecki J.

Acta Microbiologica Polonica

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1994

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tom 43

|

nr 3-4

15

Insertions within iap gene of Listeria monocytogenes generated by plamid pLIV are not lethal

58%

Bielecki J.

Acta Microbiologica Polonica

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1994

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tom 43

|

nr 2

16

Hemolytic activity of the non-ionic detergents Tween 80 and Triton X-100

44%

Bielawski J. , Mrowczynska L. , Konarczak M.

Biological Bulletin of Poznań

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1995

|

tom 32