Wyniki wyszukiwania - Biblioteka Nauki

1

Structural role of exons in hemoglobin

100%

Piwowar M. , Banach M. , Konieczny L. , Chomilier J. , Roterman I.

Bio-Algorithms and Med-Systems

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2013

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tom Vol. 9, no. 2

81--90

EN

The role of exons can be studied on many levels, one of which pertains to protein structure. It is a well-known fact that secondary structural motifs do not directly correspond to exons: helices, β-sheets and loops have all been identified as encoded by more than one exon. The relation between exon fragments and their involvement in shaping the three-dimensional (3D) structure of a protein body is subject to ongoing studies. In particular, the role of exons in stabilizing tertiary structures can be related to the structure of the hydrophobic core of the protein. Participation of specific polypeptide fragments (single exons) in hydrophobic stabilization reveals the role played by each fragment. In the course of the presented research, exons in selected proteins have been identified on the basis of GenBank files, imported from the nucleotide database at the National Center of Biotechnology Information. Amino acid sequences representing each exon were subsequently traced to parts of 3D structural forms. The participation of each exon fragment in shaping the hydrophobic core of the protein was measured using divergence entropy calculations. It was found that each protein contains at least one exon which encodes a structural fragment in accordance with the theoretical hydrophobic core model. This implies that the likely role of at least one exon in each protein is to generate a hydrophobic core which is, in turn, responsible for tertiary structural stabilization.

2

Analysis of oxygen binding by hemoglobin on the basis of mean intrinsic thermodynamic quantities

100%

Bordbar A.-K. , Mousavi S. H.-A. , Dazhampanah H.

Acta Biochimica Polonica

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2006

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tom 53

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nr 3

563-568

EN

The binding data for oxygenation of human hemoglobin, Hb, at various temperatures and in the absence and presence of 2,3-diphosphoglycerate, DPG, and inositol hexakis phosphate, IHP, were analyzed for extraction of mean intrinsic Gibbs free energy, ΔGo, enthalpy, ΔHo, and entropy, ΔSo, of binding at various partial oxygen pressures. This method of analysis considers all the protein species present such as dimer and tetramer forms which were not considered by Imai et al. (Imai K et al., 1970, Biochim Biophys Acta 200: 189 - 196) , in their analysis which was based on Adair equation. In this regard, the values of Hill equation parameters were estimated with high precision at all points of the binding curve and used for calculation of ΔGo, ΔHo and ΔSo were also calculated by analysis of ΔGo values at various temperatures using van't Hoff equation. The results represent the enthalpic nature of the cooperativity in Hb oxygenation and the compensation effect of intrinsic entropy. The interpretation of results also to be, into account the decrease of the binding affinity of sites for oxygen in the presence of DPG and IHP without any considerable changes in the site - site interaction (extent of cooperativity). In other words, the interactions between bound ligands, organic phosphates and oxygen, are more due to a decreasing binding affinity and not to the reduction of the cooperative interaction between sites. The results also document the more heterotropic effect of IHP compared to DPG.

3

Body mass dependence of the haemoglobin content to surface area ratio of avian erythrocytes

100%

Kostelecka-Myrcha A. , Cholostiakow-Gromek J.

Acta Ornithologica

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2001

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tom 36

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nr 2

EN

Data from 75 bird species weighing between 6 g and > 16 kg confirmed a supposition that the amount of haemoglobin per unit surface area of erythrocyte was not dependent on body mass. It showed a constancy across the range reflecting adjustment of the total surface area of erythrocytes in relation to blood haemoglobin concentration. This conclusion is based on an inverse correlation between the numbers and sizes of red blood cells.

PL

Zbadano zależności wartości wskaźników czerwonokrwinkowych od masy ciała ptaków. Do analizy tej włączono dane własne, uzyskane we wcześniejszych badaniach (Kostelecka-Myrcha et al. 1993) i dostępne dane literaturowe. Ogółem zebrano informacje dla 75 gatunków ptaków, o masie ciała od 6 g do ponad 16 kg. Poszczególne zależności scharakteryzowano za pomocą współczynników korelacji i regresji krzywoliniowych. Stwierdzono, że koncentracja hemoglobiny (Hb, Fig. 1) i liczba erytrocytów w 1 mm³ krwi (Fig. 2) zmniejszają się statystycznie istotnie ze wzrostem masy ciała ptaków, podczas gdy wielkość tych krwinek wzrasta (Fig. 3). Bardzo intensywne zmniejszanie się liczby erytrocytów w jednostce objętości krwi powoduje statystycznie istotne zmniejszanie się wartości hematokrytu (Fig. 4) i sumarycznej powierzchni erytrocytów (TSAE, Fig. 5). Zmiany wielkości tej powierzchni przebiegają wprost proporcjonalnie do zmian koncentracji Hb i dlatego stosunek Hb/TSAE nie zależy od masy ciała ptaków (Fig. 6). Jego średnia wartość wynosi 0.52 ± 0.09 pg μm⁻². Nie różni się ona statystycznie istotnie od wcześniej otrzymanej średniej dla 564 osobników różnych gatunków ptaków, przebadanych w zależności od wieku piskląt, sezonu roku i masy ciała, w wąskim zakresie jej zmienności — od 6 g do 167 g (Kostelecka-Myrcha 1997). Przedstawiona analiza potwierdza więc przypuszczenie z 1997 roku (ibidem), że ilość hemoglobiny przypadająca na jednostkę powierzchni erytrocytów (Hb/TSAE) jest u ptaków stała, i że stałość ta jest wynikiem dostosowywania się wielkości TSAE do koncentracji Hb. U podstaw tego ważnego mechanizmu, zapewniającego zawsze pełne wysycenie hemoglobiny tlenem, leży ujemna korelacja między liczbą a wielkością erytrocytów.

4

Antioxidant activity of hemoglobin

100%

Falcioni G. , Caulini G. C. , Fedeli D. , Santroni A. M. , Gabbianelli R. , Kantar A.

Polish Journal of Environmental Studies

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1998

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tom 07

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nr 6

5

Robust Identification Approach to Individualized Anemia Model

88%

Akabua E. , Inanc T. , Gaweda A. , Brier M. E. , Kim S. , Zurada J. M.

Journal of Applied Computer Science Methods

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2011

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tom Vol. 3 No. 2

65--75

EN

Over ninety percent of End Stage Renal Disease (ESRD) patients suffer from anemia due to insufficient endogenous production of human erythropoietin. Until the advent of Recombinant Human Erythropoietin (r-HuEPO) over 30 years ago, patients with ESRD were treated mainly with multiple blood transfusions. The high cost of r-HuEPO in addition to the narrow margin between an effective do-sage and toxicity in drug administration calls for optimal dosage strategy capable of minimizing cost and toxicity while at the same time achieving the desired do-sage outcome. It is well known from control theory that a controller can be de-signed for any plant provided there is readily available a valid model for such a plant. We present Robust Identification procedure, a dimensionality reduction technique capable of capturing the inherent dynamics of anemia patients; conse-quently producing individualized model suitable for robust control synthesis and any other controller design methodologies.

6

Comparing prevalence and characteristic of anemia in children with simple versus complex febrile seizures

88%

Momen A. A. , Asnafi A. A. , Nikkhah A. , Reza Khalilian M. , Mahmudi M. , Da voodzadeh H.

Family Medicine & Primary Care Review

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2018

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nr 1

25-28

7

The ratio of amount of haemoglobin to total surface area of erythrocytes in mammals

88%

Kostelecka-Myrcha A.

Acta Theriologica. Supplement

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2002

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tom 47

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nr 1

EN

The literature provides all the data needed to calculate the ratio between the amount of haemoglobin and the total surface area of erythrocytes in 54 species of mammals ranging in body mass from 2.5 g to more than 1000 kg. Analysis shows that the concentration of haemoglobin (Hb; g%) does not defend on the body mass ofthe mammals studied. The number of erythrocytes in 1 mm of blood (RBC; 10 x mm ) is significantly lower, and the diameter of these cells significantly higher, among larger mammals as opposed to smaller ones. The result is that the total surface area of erythrocytes in 1 mm of blood (TSAE; mm x mm ) is significantly lower among larger mammals, while the Hb/TSAE ratio (pg x ^m- ) is significantly greater. These results point to the smaller size of erythrocytes of smaller mammals permitting much greater numbers to exist, thereby producing a greater TSAE and smaller Hb/TSAE ratio. The greater total surface area of red blood cells per unit volume of blood in small mammals can in turn be presumed to allow for full saturation of haemoglobin by oxygen, even where the period of contact between erythrocytes and air in the lungs is shorter than in their larger counterparts.

8

Effects of low doses of gamma rays on the stability of normal and diabetic erythrocytes

75%

Kaczmarska M. , Kopyściańska Z. , Fornal M. , Grodzicki T. , Matlak K. , Korecki J. , Burda K.

Acta Biochimica Polonica

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2011

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tom 58

|

nr 4

489-496

EN

We studied the influence of low doses of γ radiation (from 0.04 to 1.8 mGy) on the stability of human red blood cells (RBC) from healthy donors and diabetic patients using absorption spectroscopy. Because of the alteration of many enzymatic pathways in diabetic RBCs resulting in strong modification of the lipid and protein membrane components one could expect that the ionizing γ-radiation should influence the stability of the healthy and diabetic cells in a different way. Indeed, distinct discontinuities and monotonic changes of hemolysis detected in the healthy and diabetic RBCs suggest that various enzymatic and chemical processes are activated in these membranes by γ radiation. Mössbauer measurements showed that only the highest applied dose of γ radiation caused modification of hemoglobin in both types of RBCs.

9

Krew i produkty pochodne

75%

Banach M. , Makara A.

Czasopismo Techniczne. Chemia

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2011

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tom R. 108, z. 2-Ch

3-20

PL

Omówiono zagadnienia związane z podażą krwi na polskim rynku i możliwości jej wykorzystania, zastosowania plazmy i hemoglobiny wytwarzanych z krwi.

EN

The paper relates the questions connected with supply of the blood on Polish market and the possibility of utilization, the use of plasma and hemoglobin produced from the blood.

10

Flavonoids as reductants of ferryl hemoglobin

75%

Gebicka L. , Banasiak E.

Acta Biochimica Polonica

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2009

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tom 56

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nr 3

509-513

EN

The ferryl derivatives of hemoglobin are products of the reactions of oxy- and methemoglobin with hydrogen peroxide. Ferryl hemoglobins, either with or without a radical site on the protein moiety, are oxidizing species. Plant polyphenols, flavonoids, have been shown to act as antioxidants in vivo and in vitro. Reactions of met- and oxyhemoglobin with hydrogen peroxide in the presence of catechin, quercetin and rutin were studied. These flavonoids accelerated reduction of ferryl hemoglobin to methemoglobin. The rate constants of the reactions of ferryl hemoglobin with catechin, quercetin and rutin were in the order of 102 M-1 s-1, i.e. similar to the rate constants of ferryl hemoglobin with intracellular reducing compounds like urate or ascorbate. The beneficial effect of flavonoids against oxidative damage of hemoglobin caused by hydroperoxides, reported in the literature, is probably, at least in part, connected with the ability of flavonoids to scavenge ferryl hemoglobin.

11

Elimination of goat haemoglobin and its complexes with goat haptoglobin from goat and rat circulation

75%

Osada J. , Nowacki W.

Acta Biochimica Polonica

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1989

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tom 36

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nr 3-4

12

Characteristic changes in erythrocyte nucleotides in haemolytic anaemia with basophilic stippling of differing aetiology

75%

Laurence A. , Duley J. A. , Simmonds H. A. , Layton M.

Cellular and Molecular Biology Letters

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1999

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tom 04

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nr 3

13

Influence of alcohol radicals on the level of dissociation and functional characteristics of human hemoglobin

75%

Puchala M. , Serafin E. , Szweda-Lewandowska Z.

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tom 18

|

nr 2

14

The efficacy of endogenous and exogenous antioxidant protection of erythrocytes against the action of radical generating factors [ageing in vitro]

75%

Kopec-Szlezak J. , Grabarczyk M. , Wozniak J.

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tom 18

|

nr 2

15

Interactions between different inhibitors of the anion transport system in human red blood cell membrane

75%

Zaki L.

Biophysics of Membrane Transport

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1994

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tom 12

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nr 2

16

Lysine-specific gingipain K and heme-hemoglobin receptor HmuR are involved in heme utilization in Porphyromonas gingivalis

75%

Simpson W. , Olczak T. , Genco C. A.

Acta Biochimica Polonica

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2004

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tom 51

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nr 1

EN

We have previously reported on the identification and characterization of the Porphyromonas gingivalis A7436 strain outer membrane receptor HmuR, which is involved in the acquisition of hemin and hemoglobin. We demonstrated that HmuR interacts with the lysine- (Kgp) and arginine- (HRgpA) specific proteases (gingipains) and that Kgp and HRgpA can bind and degrade hemoglobin. Here, we report on the physiological significance of the HmuR-Kgp complex in heme utilization in P. gingivalis through the construction and characterization of a defined kgp mutant and a hmuR kgp double mutant in P. gingivalis A7436. The P. gingivalis kgp mutant exhibited a decreased ability to bind both hemin and hemoglobin. Growth of this strain with hemoglobin was delayed and its ability to utilize hemin as a sole iron source was diminished as compared to the wild type strain. Inactivation of both the hmuR and kgp genes resulted in further decreased ability of P. gingivalis to bind hemoglobin and hemin, as well as diminished ability to utilize either hemin or hemoglobin as a sole iron source. Collectively, these in vivo results further confirmed that both HmuR and Kgp are involved in the utilization of hemin and hemoglobin in P. gingivalis A7436.

17

New generation of peptide antibiotics

63%

Dubin A. , Mak P. , Dubin G. , Rzychon M. , Stec-Niemczyk J. , Wladyka B. , Maziarka K. , Chmiel D.

Acta Biochimica Polonica

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2005

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tom 52

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nr 3

633-638

EN

The increasing antibiotic resistance of pathogenic bacteria calls for the development of alternative antimicrobial strategies. Possible approaches include the development of novel, broad-spectrum antibiotics as well as specific targeting of individual bacterial virulence factors. It is impossible to decide currently which strategy will prove more successful in the future since they both promise different advantages, but also introduce diverse problems. Considering both approaches, our laboratory's research focuses on the evaluation of hemocidins, broad-spectrum antibacterial peptides derived from hemoglobin and myoglobin, and staphostatins, specific inhibitors of staphopains - Staphylococcus aureus secreted proteases that are virulence factors regarded as possible targets for therapy. The article summarizes recent advances in both fields of study and presents perspectives for further development and possible applications.

18

Plant hemoglobins can be maintained in active form by reduced flavins and confer tolerance to nitro-oxidative stress

63%

Sainz M. , Perez-Rontome C. , Ramos J. , Mulet J. , James E. , Becana M.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

|

nr 2

19

Exploiting iron-binding proteins for drug delivery

63%

Bialasek M. , Kubiak M. , Gorczak M. , Braniewska A. , Kucharzewska-Siembieda P. , Krol M. , Taciak B.

Journal of Physiology and Pharmacology

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2019

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tom 70

|

nr 5

20

Środowiskowe implikacje zagospodarowania krwi poubojowej jako odpadu z przemysłu mięsnego

63%

Kasprzak J.

Inżynieria i Aparatura Chemiczna

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2017

|

tom Nr 5

168--169

PL

Przedstawiono problematykę zagospodarowania poubojowej krwi zwierzęcej (wieprzowo-wołowej i drobiowej). Scharakteryzowano podstawowe zagadnienia dotyczące powstawania i zagospodarowania poubojowej krwi zwierzęcej. Przedstawiono technologiczne możliwości wykorzystania krwi oraz jej pochodnych (plazmy krwi i hemoglobiny) w różnych gałęziach gospodarki, w tym w przemyśle paszowym. Podjęto również próbę oceny skutków takich zabiegów dla środowiska (z wykorzystaniem metody oceny cyklu życia) w porównaniu z utylizacją tej grupy odpadów.

EN

Problems dealing with animal slaughter blood disposal (pork- beef and poultry) are described. Key issues concerning formation and disposal of slaughter blood are characterized. Technological possibilities of using blood and its derivatives (blood plasma and hemoglobin) are demonstrated in a variety of applications, including those in feed industry. An attempt of assessment of environmental effects of such treatment using a lifecycle assessment method compared with the utilization of this waste was also performed.