Wyniki wyszukiwania - Biblioteka Nauki

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Two new cases of KIR3DP1, KIR2DL4-negative genotypes, one of which is also lacking KIR3DL2

100%

Niepieklo-Miniewska W. , Zuk N. , Dubis J. , Kurpisz M. , Senitzer D. , Havrylyuk A. , Grendziak R. , Witkiewicz W. , Chopyak V. , Kusnierczyk P.

Archivum Immunologiae et Therapiae Experimentalis

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2014

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tom 62

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nr 5

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Three novel single nucleotide polymorphisms of the 3-hydroxy-3-methylglutaryl coenzyme A reductase gene associated with egg-production in chicken

86%

Han C. , An G. , Du X.

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2014

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tom 62

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nr 3

3

Molecular identification of Paramecium bursaria syngens and studies on geographic distribution using mitochondrial cytochrome C oxidase subunit I (COI)

86%

Zagata P. , Greczek-Stachura M. , Tarcz S. , Rautian M.

Folia Biologica

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2015

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tom 63

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nr 1

4

Effects of common INSIG-2 and SCAP gene variants on metabolic parameters in coronary artery disease

86%

Coban D. , Eronat A. P. , Ceviz A. B. , Coskunpinar E. , Bugra Z. , Yilmaz-Aydogan H. , Ozturk O.

Folia Biologica

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2017

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tom 65

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nr 4

5

The evaluation of bovine SNP50 beadchip assay performance in Polish red cattle breed

86%

Gurgul A. , Zukowski K. , Pawlina K. , Zabek T. , Semik E. , Bugno-Poniewierska M.

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nr 3-4

6

Mass spectrometry approaches in proteomic and metabolomic studies

86%

Rodziewicz P. , Swarcewicz B. , Chmielewska K.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2014

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tom 95

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nr 3

7

Exploration of metagenomes for new enzymes useful in food biotechnology - a review

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Urban M. , Adamczak M.

Polish Journal of Food and Nutrition Sciences

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2008

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tom 58

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nr 1

EN

Metagenomics is the genomic analysis of the collective genomes of an assemblage of organisms, or the metagenome. Metagenomic analysis has been applied to diverse problems in microbiology and has yielded insight into the physiology of uncultured organisms to access the potentially useful enzymes and secondary metabolites they produce. DNA isolation methods have to be strictly adapted to the type of isolated biological material; of great importance is also the size of the obtained DNA. Small DNA fragments are usually sufficient for an analysis of individual genes or their small groups, whereas large inserts are required for analysing metabolic pathways, genome structures or sequencing large DNA fragments. There are two types of methods of extracting genomic DNA. One of them consists of the direct extraction of nucleic acids from an environmental sample after the cell lysis (in situ), followed by purification of the obtained DNA. The other method consists of the separation of bacterial cells from an environmental sample, followed by lysis of the cell suspension and DNA extraction. In the presented review methods of the environmental DNA isolation, cloning and new enzymes discovery are presented.

PL

Metagenomika obejmuje analizę wszystkich genomów (metagenom) organizmów w danym środowisku. Zastosowanie analizy metagenomicznej w badaniach mikrobiologicznych pozwoliło zrozumieć fizjologię drobnoustrojów nienamnażanych z użyciem standardowych metod, tym samym umożliwiając pozyskiwanie nowych enzymów i metabolitów wtórnych. Metody izolacji DNA muszą być ściśle dostosowane do rodzaju izolowanego materiału biologicznego i determinują jakość i wielkość otrzymanego DNA. Małe fragmenty DNA są zwykle wystarczające do analizy pojedynczych lub małych grup genów, podczas gdy duże inserty wymagane są do analizy szlaków metabolicznych, organizacji genomu czy sekwencjonowania dużych fragmentów DNA. Obecnie wyróżnia się dwa rodzaje metod ekstrakcji genomowgo DNA. Pierwsza grupa metod polega na bezpośredniej ekstrakcji kwasów nukleinowych z próbki środowiskowej po uprzedniej lizie (in situ), a następnie oczyszczeniu otrzymanego DNA. Metody pośrednie polegają na wydzieleniu komórek bakteryjnych z próbki środowiskowej, a następnie lizie zawiesiny komórek i dalej ekstrakcji DNA. W publikacji przedstawiono różne metody izolacji genomowego DNA ze środowiska naturalnego, metody jego klonowania oraz zaprezentowano przykłady pozyskiwania nowych enzymów z metagenomu.

8

Differentiation of Toxocara canis and Toxocara cati based on PCR-RFLP analyses of rDNA-ITS and mitochondrial cox1 and nad1 regions

72%

Mikaeili F. , Mathis A. , Deplazes P. , Mirhendi H. , Barazesh A. , Ebrahimi S. , Kia E. B.

Acta Parasitologica

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2017

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tom 62

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nr 3

9

An improved method for efficient isolation and purification of genomic DNA from filamentous cyanobacteria belonging to genera Anabaena, Nodularia and Nostoc

72%

Kaczynska A. , Los M. , Wegrzyn G.

Oceanological and Hydrobiological Studies

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2013

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tom 42

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nr 1

10

Demographic versus genetic [RAPD] variation between and within two populations of the clonal plant Paris quadrifolia L. [Liliaceae]

72%

Kosinski I. , Stefanowicz-Hajduk J. , Ochocka R.

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nr 2

303-311

EN

The two populations of Paris quadrifolia L. were studied in isolated habitats in a currently fragmented landscape. Both populations were located in deciduous forests, the first (A) – on fresh mineral and acidic soil, and the second (B) – on wet organogenic, less acidic and more fertile soil. We hypothesized that genetic variation should be higher within population of more fecund plants, and that genetic distance between the two populations that occupy different isolated habitats in a fragmented landscape should be high. Demographic characteristics of populations were studied in the 2000–2005 period. In patches of both populations, 22 permanent plots measuring 1m² each were designated. For molecular testing 41 samples from both populations were selected. The share of generative shoots was higher in the population A than population B (0.35 and 0.20, respectively). However, the fecundity of ramets expressed as the number of seeds in the ripe fruit was lower in A than in the B population (15 versus 21 seeds). The germination ability was significantly higher for the seeds from A than from the B population (79% versus 44%). The survival of the juveniles was high in both populations (54 and 76%). The Random Amplified Polymorphic DNA (RAPD) analysis with the application of five primers permitted identifying 91 loci. The estimation of genetic diversity was based on polymorphic loci, the share of which was average 44%. Nei’s gene diversity (h) was higher in the A than B population (0.28 versus 0.22). The genetic diversity between the populations was not large (GST = 0.14). Clonal diversity was very high, G/N ratio = 1, and cluster analysis showed intermingling between samples from both the populations. There were quite a small genetic distance (D = 0.10), and a rather high level of gene flow (Nm = 1.51) between the populations from currently isolated habitats. The obtained results indicate that the genetic diversity was lower within population of more fecund plants from more productive habitat.

11

Paramecium jenningsi species complex (Ciliophora, Protista) in India: the first description of new stands of P. bijenningsi and P. trijenningsi

72%

Przybos E. , Kalavati C. , Fokin S. I. , Tarcz S.

Folia Biologica

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2017

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tom 65

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nr 1

12

Characterization of the protein gp1 from bacteriophage phiIN93

72%

Matsushita I. , Yanase H.

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tom 48

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nr 1

13

Genetic characterization of Toxoplasma gondii isolates from chickens in India by GRA6 gene sequence analysis

72%

Biradar S. S. , Saravanan B. C. , Tewari A. K. , Sreekumar C. , Sankar M. , Sudhakar N. R.

Acta Parasitologica

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2014

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tom 59

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nr 4

EN

PCR-RFLP and nucleotide sequencing based genotyping of Toxoplasma gondii Indian isolates (Izatnagar and Chennai isolates and Chennai clone) vis-a vis RH-IVRI strain was conducted by targeting GRA6 as genetic marker. The 791 bp GRA6 product was PCR amplified from the genomic DNA of different T. gondii Indian isolates, including the RH-IVRI strain. Tru1I restriction endonuclease based PCR-RFLP of GRA6 sequence produced polymorphic digestion pattern that discriminated the virulent RH-IVRI strain (as type I) from the moderately virulent local isolates as type III. The PCR amplicon of T. gondii GRA6 from RH-IVRI strain as well as from the local isolates were cloned in cloning vector and custom sequenced. The nucleotide and deduced amino acid sequences of T. gondii isolates were aligned with that of the type I, II and III strains (RH, BEVERLEY, ME49, C56, TONT and NED) available in public domain and analyzed in silico using MEGA version 4.0 software. Nucleotide sequencing and phylogenetic analysis of GRA6 marker from the Indian isolates revealed a close genetic relationship with type III strains of T. gondii. Further, detection of a single nucleotide polymorphism (SNP) at positions 162 and 171 of the GRA6 marker, established the lineage of Indian isolates as type III. This is the first report on characterization of T. gondii lineage as type III in selective chicken population of India based on PCR-RFLP and sequence analysis of GRA6 gene.

14

In vitro and molecular characterization using ISSR markers of Glycyrrhiza glabra L.

72%

El-Hameid A. A. , El-Kheir Z. A. , Abdel-Hady M. , Helmy W.

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2018

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tom 99

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nr 4

15

New genetic lineages, host associations and circulation pathways of Neorickettsia endosymbionts of digeneans

72%

Tkach V. V. , Schroeder J. A. , Greiman S. E. , Vaughan J. A.

Acta Parasitologica

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2012

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tom 57

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nr 3

EN

Neorickettsia is a genus of intracellular bacteria endosymbiotic in digeneans that may also invade cells of vertebrates and are known to cause diseases of wildlife and humans. Herein, we report results of screening for Neorickettsia of an extensive collection of DNA extracts from adult and larval digeneans obtained from various vertebrates and mollusks in the United States. Seven isolates of Neorickettsia were detected by PCR and sequenced targeting a 527 bp long region of 16S rRNA. Sequence comparison and phylogenetic analysis demonstrated that four isolates matched published sequences of Neorickettsia risticii. Three other isolates, provisionally named “catfish agents 1 and 2” (obtained from Megalogonia ictaluri and Phyllodistomum lacustri, both parasitic in catfishes) and Neorickettsia sp. (obtained from cercariae of Diplostomum sp.), differed from previously known genotypes of Neorickettsia and are likely candidates for new species. All 7 isolates of Neorickettsia were obtained from digenean species and genera that were not previously reported as hosts of these bacteria. Members of four digenean families (Dicrocoeliidae, Heronimidae, Macroderoididae and Gorgoderidae) are reported as hosts of Neorickettsia for the first time. Our study reveals several new pathways of Neorickettsia circulation in nature. We have found for the first time a Neorickettsia from a digenean (dicrocoeliid Conspicuum icteridorum) with an entirely terrestrial life cycle. We found N. risticii in digeneans (Alloglossidium corti and Heronimus mollis) with entirely aquatic life cycles. Previously, this Neorickettsia species was known only from digeneans with aquatic/terrestrial life cycles. Our results suggest that our current knowledge of the diversity, host associations and circulation of neorickettsiae is far from satisfactory.

16

The estimation of genetic variation within and between Polish provenances of Norway spruce (Picea abies (L.) Karst.) on the basis of RAPD polymorphism

72%

Kraj W.

Electronic Journal of Polish Agricultural Universities. Series: Forestry

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2002

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tom 05

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nr 2

17

Characterization of a gene cluster coding for cinnamomin and three related elicitins in Phytophthora cinnamomi

72%

Duclos J. P. , Cravador A. , Coelho A. C. , Bollen A. , Fauconnier A. , Godfroid E.

Biological Bulletin of Poznań. Supplement

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1997

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tom 34

18

DNA methylation - an essential mechanism in plant molecular biology

72%

Hafiz I. A. , Anjum M. A. , Grewal A. G. , Chaudhary G. A.

Acta Physiologiae Plantarum

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2001

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tom 23

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nr 4

19

A method of DNA isolation using a commercial washing powder

72%

Brzuzan P.

Polskie Archiwum Hydrobiologii

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1997

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tom 44

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nr 3

20

Immune response against genetically modified tumour cells

72%

Nowak J. , Januszkiewicz D. , Kowalczyk D. , Mazurek J. , Laciak M. , Malicki J. , Murawa P. , Wiznerowicz M. , Heinrich P. C. , Rose-John S. , Mackiewicz A.

Biotechnologia

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1996

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nr 4