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EN
This study defines several mycotoxin (Aflatoxin B1, Aflatoxin B2, Aflatoxin G1, Aflatoxin G2, Ochratoxin A, Deoxynivalenol, Zearalenone, Toxin T-2, Toxin HT-2, Nivalenol, Fusarenon X, 3-Acetyl-deoxynivalenol) contamination of winter common, durum, spelt and einkorn wheat genotypes. The compared species (Triticum aestivum ssp. vulgare, T. durum, T. aestivum ssp. spelta and T. monococcum) have different susceptibility to Fusarium and toxin accumulation. Durum wheat (cv. Komnata) was the most susceptible to contamination with mycotoxins. In durum grain the highest level of contamination was detected, especially with Deoxynivalenol (2–4 times over the allowed level for unprocessed grain). T. aestivum ssp. spelta (cv. Schwabenkorn) and T. monococcum (EN 5003) showed the lowest mycotoxin level. Triticum aestivum ssp. vulgare (cv. Tonacja) was less contaminated with mycotoxins than Triticum durum but more than T. aestivum ssp. spelta and T. monococcum. Other mycotoxins in grain of the examined genotypes occurred in trace amounts.
EN
Wheat quality depends directly on the grain protein content and protein composition. High and low molecular weight glutenin subunits play an important role in determining the visco-elastic properties of gluten. In an attempt to improve the breadmaking quality of hexaploid triticale, a fragment of wheat chromosome 1D, containing the Glu-D1 allele encoding the 5+10 subunits, was translocated to the long arm of chromosome 1A by Lukaszewski and Curtis [1], The 1A.1D translocation chromosome was transferred to tetraploid wheat [2], making the Glu-D1 locus available for the improvement of durum wheat. The goal of this study was to evaluate using cytogenetics and molecular approaches the amount of chromatin introgressed in durum wheat. Fluorescence in situ hybridization with total genomic DNA (GISH) of Aegilops squarrosa L. indicated that the translocated chromosome 1A.1D had a terminal 1DL segment of about 35-40% of the recombinant arm length. Several pairs of microsatellite primers from chromosome 1A and 1D were used to genetically characterize the recombinant chromosome. The mapping data indicated that a 1AL segment, at least 150 cM long, was substituted by a 1DL segment with a minimal length of 72 cM, and that the translocation breakpoint was near the 1A centromeric region. The genetic and physical data highlight a substantial discrepancy between the recombinational and physical map distances. We are using a targeted strategy via the Ph pairing manipulation system to generate smali intercalary 1D chromosome segments in a durum wheat background.
EN
A set of recombinant inbred lines (RIL) derived from a cross between the cultivar Messapia of durum wheat (Triticum turgidum var. durum) and the accession MG4343 of T. turgidum var. dicoccoides was analysed to increase the number of assigned markers and the resolution of the previously constructed genetic linkage map. An updated map of the durum wheat genome consisting of 458 loci was constructed. These loci include 261 Restriction Fragment Length Polymorphisms (RFLPs), 91 microsatellites (Simple Sequence Repeats, SSRs), 87 Amplified Fragment Length Polymorphisms (AFLPs), two ribosomal genes, and nine biochemical (seven seed storage proteins and two isozymes) and eight morphological markers. The loci were mapped on all 14 chromosomes of the A and B genomes, and covered a total distance of 3038.4 cM with an average distance of 6.7 cM between adjacent markers. The molecular markers were evenly distributed between the A and the B genomes (240 and 218 markers, respectively). An additional forty loci (8.8%) could not be assigned to a specific linkage group. A fraction (16.4%) of the markers significantly deviated from the expected Mendelian ratios; clusters of loci showing distorted segregation were found on the 1B, 2A, 2B, 3A, 4A, 7A and 7B chromosomes. The genetic lengths of the chromosomes range from 148.8 cM (chromosome 6B) to 318.0 cM (chromosome 2B) and approximately concur with their physical lengths. Chromosome 2B has the largest number of markers (47), while the chromosomes with the fewest markers are 3A and 6B (23). There are two gaps larger than 40 cM on chromosomes 2A and 3B. The durum wheat map was compared with the published maps of bread and durum wheats; the order of most common RFLP and SSR markers on the 14 chromosomes of the A and B genomes were nearly identical. A core-map can be extracted from the highdensity Messapia x dicoccoides map and a subset of uniformly distributed markers can be used to detect and map quantitative trait loci.
EN
The objective of this study was to determine the abundance and species composition of thrips collected from ears of Triticum durum Desf. in relation to plant protection intensity. The experiment was set up at the Experimental Research Station of Wrocław University of Environmental and Life Sciences, at Pawłowice (51°09’ N; 17°06’ E), Poland, in 2006-2008. It was conducted on durum wheat plants at three different levels of plant protection intensity and on common winter wheat plants. The samples of thrips were collected from the ears of the plants at the milk maturity stage of the grain. During three years of the research, eight species of thrips were identified from Triticum durum. Most often the eudominants were Haplothrips aculeatus, Limothrips cerealium and L. denticornis. We have demonstrated that there was no statistically significant effect of the plant protection intensity on the thrips abundance. The host species, on the other hand, did not affect the species composition of the studied insects.
PL
Celem badań było poznanie liczebności i składu gatunkowego przylżeńców (Thysanoptera) występujących w kłosach pszenicy twardej (Triticum durum Desf.) w zależności od intensywności ochrony tej rośliny. Doświadczenie prowadzono w latach 2006-2008, w Rolniczym Zakładzie Doświadczalnym w Pawłowicach (51°09’ N; 17°06’ E), należącym do Uniwersytetu Przyrodniczego we Wrocławiu. Badaniami objęto pszenicę twardą bez ochrony chemicznej, chronioną środkami chemicznymi oraz porównawczo pszenicę zwyczajną. W celu określenia składu gatunkowego przylżeńców pobierano kłosy, których ziarniaki były w fazie dojrzałości mlecznej. W ciągu trzech lat badań w kłosach pszenicy twardej oznaczono 8 gatunków przylżeńców. Do eudominantów najczęściej należały Haplothrips aculeatus, Limothrips cerealium i L. denticornis. Nie stwierdzono wyraźnego wpływu zastosowanej ochrony roślin na liczebność badanej grupy owadów na pszenicy twardej. W kłosach pszenicy twardej występowały te same gatunki przylżeńców jak w kłosach pszenicy zwyczajnej.
EN
Phylogenetic relationships among wild Triticum and Aegilops species, bread wheat (Triticum aestivum) cultivars, and durum wheat (T. turgidum) cultivars were investigated using random amplified polymorphic DNA (RAPD) technique. Fourteen RAPD primers generated 328 polymorphic bands in 22 wheat species/cultivars which have the same or different genomes. DNA fragment size ranged from 290 bp to 2570 bp. In the RAPD analysis, wild Triticum and Aegilops species clustered together and were separated from all other wheat cultivars based on their genome constitution. T. monococcum and T. boeticum were closer to Aegilops species than to other wheat cultivars. T. turgidum cultivars were genetically less diverse than T. aestivum cultivars. RAPD markers specific to the D and U genomes were detected. There was a weak correlation between RAPD data and pedigree records of the cultivars sharing common ancestor(s). The results suggest that RAPD analysis can be used to distinguish wild Triticum and Aegilops species, and wheat cultivars. In addition, RAPD technique can be used to develop genome-specific markers.
EN
A field experiment was conducted at the Experimental Station Krasne near Rzeszów in the years 2010–2012. The experiment was established in the split-plot design including the control treatment in 4 replications, on a soil classified as the good wheat complex. The aim of this study was to estimate and compare an effect of several methods of weed control, i.e. mechanical with harrowing , chemical (with the recommended and lowered dose of a herbicide) and combining both of those methods with a reduced dose of a herbicide on the state and degree of weed infestation of durum wheat (Triticum durum Desf.) and common wheat (T. aestivum L.) cultivars, Harrowing of durum wheat and common wheat crops, combined with spraying with a dose of the herbicide Granstar Ultra SX 50 SG reduced by a half, limited weed infestation as effectively as the chemical method with the recommended dose of a herbicide. A good effectiveness of harrowing in weed control, including the predominant taxons Chenopodium album and Stellaria media, was obtained in both durum wheat and common wheat cultivars crops. Durum wheat cultivars Durobonus and Duroflavus proved to be less competitive in relation to weeds as compared with common wheat. A similar species composition was observed in the weed communities of tested cultivars, and differences concerned the amounts and air-dry weight of weed species.
PL
Doświadczenie polowe prowadzono w Stacji Doświadczalnej Krasne koło Rzeszowa w latach 2010–2012. Eksperyment założono metodą split-plot z obiektem kontrolnym, w 4 powtórzeniach, na glebie zaliczanej do kompleksu pszennego dobrego. Celem badań było określenie i porównanie stanu oraz stopnia zachwaszczenia łanów odmian pszenicy twardej (Triticum durum Desf.) i pszenicy zwyczajnej (T. aestivum L.), w których stosowano różne sposoby regulacji zachwaszczenia: mechaniczny z bronowaniem zasiewów, chemiczny z zalecaną i obniżoną dawką herbicydu oraz połączenie metody mechanicznej i chemicznej z obniżoną dawką herbicydu. Bronowanie zasiewów pszenicy twardej i zwyczajnej połączone z opryskiwaniem zmniejszoną o połowę dawką herbicydu Granstar Ultra SX 50 SG ograniczało zachwaszczenie równie skutecznie, jak metoda chemiczna z zalecaną dawką herbicydu. Uzyskano dobrą skuteczność bronowania w zwalczaniu chwastów, w tym taksonów dominujących: Chenopodium album i Stellaria media, zarówno w zasiewach odmian pszenicy twardej, jak i zwyczajnej. Odmiany pszenicy twardej: Durobonus i Duroflavus okazały się mniej konkurencyjne w stosunku do chwastów w porównaniu z pszenicą zwyczajną. W strukturze florystycznej zbiorowisk chwastów poszczególnych odmian zaobserwowano podobny skład gatunkowy, a różnice dotyczyły ilości i powietrznie suchej masy występujących gatunków chwastów.
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