Wyniki wyszukiwania - Biblioteka Nauki

1

Stability-indicating RP-HPLC assay for simultaneous determination of chlorpheniramine maleate and prednisolone in veterinary injection

100%

Ali A. , Farooq U. , Ahmed M. , Athar M. M. , Salman M. , Arif S. , Nadeem K. , Naz H.

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tom Vol. 32, no. 2

122--127

EN

The present work described a simple, rapid, sensitive, accurate, and precise method for simultaneous determination of chlorpheniramine maleate (CHRM) and prednisolone acetate (PRED) in injection samples by high-performance liquid chromatography (HPLC) coupled with UV–Vis detection. Chromatographic separation was accomplished, employing isocratic mode and a mobile phase comprised of acetonitrile and a phosphate buffer (50:50, v/v, 30 °C), adjusted to pH 3.0. The flow rate used was 1.0 mL/min on a Thermo Hypersil ODS C18 column (5 μm, 4.6 × 250 mm), and the injection volume of sample was 20 μL. Analysis of CHRM and PRED was performed at a wavelength of 254 nm. The runtime for analysis was 12.5 min, and the retention times of CHRM and PRED were found to be 2.81 and 5.07 min, respectively. The calibration graph showed linearity over the concentration range 10–70 μg/mL for CHRM and 20–140 μg/mL for PRED with a coefficient of determination (R2) ≥0.9986. Repeatability and reproducibility (expressed as % RSD) were lower than 1.72 and 1.47%, respectively. The proposed HPLC method was demonstrated to be simple and rapid for the determination of CHRM and PRED in injection formulation, providing recoveries between 101.6–102.3%, whereas complete separation of degradation products, from analyte under investigation, provided the specificity of the proposed HPLC method.

2

High efficacy of methotrexate in patients with recurrent idiopathic acute anterior uveitis: a prospective study

86%

Bachta A. , Kisiel B. , Tlustochowicz M. , Raczkiewicz A. , Rekas M. , Tlustochowicz W.

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nr 1

3

New developments in the treatment of COPD: comparing the effects of inhaled corticosteroids and N-acetylcysteine

86%

Van Overveld F. J. , Demkow U. , Gorecka D. , De Backer W. A. , Zielinski J.

Journal of Physiology and Pharmacology. Supplement

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2005

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tom 56

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nr 4

135-142

EN

Inhaled corticosteroids (ICS) are widely used for the treatment of COPD despite of controversial statements concerning their efficacy. The use of N-acetylcysteine (NAC), a mucolytic drug with antioxidant properties, is less clear, but it may counteract the oxidant-antioxidant imbalance in COPD. The aim of this study was to evaluate whether treatment of COPD patients with ICS or NAC is able to improve inflammatory indices and to enhance lung function. ICS treatment enhanced protective markers for oxidative stress such as glutathione peroxidase (GPx) (51.2 ±5.8 vs. 62.2 ±8.6 U/g Hb, P<0.02) and trolox-equivalent antioxidant capacity (TEAC) (1.44 ±0.05 vs. 1.52 ±0.06 mM, P<0.05). NAC decreased sputum eosinophil cationic protein (318 ±73 vs. 163 ±30 ng/ml, P<0.01) and sputum IL-8 (429 ±80 vs. 347 ±70 ng/ml, P<0.05). The increased antioxidant capacity prevented an up-regulation of adhesion molecules, since the levels of intracellular adhesion molecule 1 (ICAM-1) correlated negatively with GPx (P<0.0001) and TEAC (P<0.0001). On the other hand, expression of adhesion molecules was promoted by inflammation, reflected by a positive correlation between the levels of IL-8 and ICAM-1 (P<0.0001). The effects of treatment on lung function were only reflected in the FEV1 values. The absolute value of FEV1, both before and after salbutamol inhalation, increased from 1690 ±98 to 1764 ±110 ml, and 1818 ±106 to 1906 ±116 ml, respectively, after ICS (P<0.05) . Ten weeks after treatment, FEV1 values dropped to 1716 ±120 ml post-salbutamol (P<0.05). When followed by treatment with NAC, these values decreased even further to 1666 ±84 ml. These results suggest that ICS improved lung function in COPD patients with moderate airflow obstruction, beside a minor improvement in the oxidant-antioxidant imbalance leading to a lesser expression of ICAM-1. Treatment with NAC decreased some inflammatory parameters and had indirectly an inhibitory effect on the expression of adhesion molecules.

4

Effects of inhaled corticosteroids on cough in awake guinea pigs with experimental allergic rhinitis - the first experience

86%

Brozmanova M. , Calkovsky V. , Plevkova J. , Tatar M.

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nr 3

23-30

EN

Allergic rhinitis is a common cause of chronic cough. Topical corticosteroids are regarded as the most effective first-time treatment in allergic rhinitis. In this study we evaluated the cough sensitivity during the early and late allergic responses in guinea pigs with experimental allergic rhinitis. Another aim of the study was to follow up the effect of inhaled beclomethasone dipropionate on the cough in guinea pigs with allergic rhinitis. 31 guinea pigs were sensitized with ovalbumin (OA). Animals were intranasally challenged with OA (experiment) or saline (control) in 7-day intervals for 9 weeks. Cough was induced by inhalation of citric acid aerosols in gradually increasing concentrations for 30 s and was evaluated 1 h after the 8th nasal challenge (NCH) and 17 h after the 9th NCH. Cough was significantly increased only during an early allergic response, 1 h after repeated NCH [18 (14-23) vs. 8 (3-10); P<0.001]. Five experimental animals were inhaling aerosol of beclomethasone dipropionate seven days between the 8th and the 9th NCH and cough was evaluated 1 h after the 9th NCH. Inhaled corticosteroids significantly inhibited the enhanced allergic rhinitis related cough [4 (1-9) vs.19 (9-37) vs. 6 (3-9); P<0.05].

5

Are we right to consider mesenchymal stem cells to be a new perspective for patients with juvenile idiopathic arthritis?

72%

Orczyk K. , Smolewska E.

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tom 66

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nr 4

6

Effects of budesonide on the lung functions, inflammation and apoptosis in a saline-lavage model of acute lung injury

72%

Mokra D. , Kosutova P. , Balentova S. , Adamkov M. , Mikolka P. , Mokry J. , Antosova M. , Calkovska A.

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nr 6

7

Using of corticosteroids in small animals ophthalmology

72%

Trbolova A.

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2014

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nr 1

8

Collateral sensitivity to gemcitabine of P-GP and MRP overexpressing human non-small cell lung cancer [NSCLC] cell lines: a possible role of corticosteroids

72%

Bergman A. M. , Pinedo H. M. , Loves W. J. P. , Veerman G. , Peters G. J.

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nr 3

9

Eosinophil cationic protein in serum of corticosteroid-sensitive and resistant bronchial asthma

72%

Patkowski J. , Wytrychowski K. , Medrala W.

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nr 2-3

10

Treatment of autoimmune disease: time for a paradigm shift?

72%

Mor F.

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nr 1

11

Wegener's granulomatosis - autoimmunity to neutrophil proteinase 3

72%

Watorek E. , Boratynska M. , Klinger M.

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2003

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tom 51

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nr 3

12

Immunopathogenesis of bronchial asthma

58%

Buc M. , Dzurilla M. , Vrlik M. , Bucova M.

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tom 57

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nr 5

331-344

13

Single-dose versus two-dose dexamethasone effects on lung inflammation and airway reactivity in meconium-instilled rabbits

58%

Mokra D. , Mokry J. , Drgova A. , Bulikova J. , Petraskova M. , Calkovska A.

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tom 58

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nr 5

14

Intratracheally administrated corticosteroids improve lung function in meconium instilled rabbits

58%

Mokra D. , Mokry J. , Drgova A. , Petraskova M. , Bulikova J. , Calkovska A.

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tom 58

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nr 5

15

The effect of beta-estradiol and corticosteroids on chlorophylls and carotenoids content in Wolffia arrhiza [L.] Wimm. [Lemnaceae] growing in municipal Bialystok tap water

58%

Czerpak R. , Szamrej I. K.

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tom 12

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nr 6

EN

It is well known that many steroid compounds, mainly from large stock-raising farms, more frequently end up in rural or communal sewage systems. It is also known that the duckweed family (Lemnaceae), especially Wolffia arrhiza, is more and more commonly used in the biotechnology of purifying the above-mentioned sewage systems due to its heterotrophic and detoxication ability, as well as its ease of adoption to unfavorable environmental conditions. Therefore, our research analyzes the influence of beta-estradiol and chemically and functionally diversified corticosteroids: cortisone, cortisole (glucocorticoids), "-deoxycorticosterone (miner- alocorticoids) and prednisolone (chemical derivative of hydrocortisone) on chlorophylls and carotenoids content in photoautotrophic Wolffia arrhiza (Lemnaceae), growing in municipal Białystok tap water (rich in minerals but poor in organic components). From the applied steroid hormones in optimal concentration of 10-6M p-es- tradiol caused the strongest stimulatory effect on photosynthetic pigments, a little less strong - cortisone, slight stimulative - cortisole, and weak "-deoxycorticosterone. Prednisolone showed a weak inhibitory influence on all types of chlorophylls and carotenoids in comparison with the control culture without exogenous hormones. Applied steroid hormones had a weak stimulative influence over chlorophylls a and b in Wolffia; the strongest was p-estradiol between the 5th and the 10th day of cultivation, in the range of 116.5-121.3% in comparison to the control value (100%). The researched steroids had a much stronger influence on carotenoid content, especially p-carotene, alloxanthin (oxygen - poor xanthophylls) and violaxanthin (oxygen - rich xanthophylls). Under the influence of beta-estradiol the amount of p-carotene rose by the maximum 160.6%, alloxanthin by 187.9% and violaxanthin by 154.3% in comparison to the control. Our research results demonstrated that p-estradiol and - from applied corticosteroids - cortisone and hydrocorticosterone, had more stimulatory influence on carotenoid content in Wolffia arrhiza, but less stimulatory effect on unicellular Chlorella vulgaris.

16

The effect of sex steroids and corticosteroids on the content of soluble proteins, nucleic acids and reducing sugars in Wolffia arrhiza [L.] Wimm. [Lemnaceae]

58%

Szamrej I. K. , Czerpak R.

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nr 5

EN

Research on the influence of sex steroids: ß-estradiol, testosterone and corticosteroids: cortisone, cortisole (glucocorticoids), 11-deoxycorticosterone (mineralocorticoid) and prednisolone (chemical derivative of hydrocortisone) on changes of soluble proteins, nucleic acids and reducing sugars as a content of Wolffia arrhiza ( Lemnaceae ) has been conducted. Wolffia has been cultivated in Bialystok’s municipal tap water (rich in mineral but poor in organic components) during a 20-day period, in the optimal concentration of 10-5 to 10-6 M. It has been ascertained that the maximal stimulation of nucleic acids (DNA & RNA) was caused by ß-estradiol in the range from 176-181%, testosterone 168-173%, cortisone 154-157%, 11-deoxycorticosterone 152-155%, cortisole 141-148% and prednisolone from 129-131%, in comparison to a 100% control. The soluble proteins content was stimulated the strongest by ß-estradiol - 181%, testosterone - 170%, cortisole - 141%, cortisone - 138%, prednisolone - 137%, and weaker by 11-deoxycorticosterone - 128%. Reducing sugars content was stimulated most intensely only on the 5th day of cultivation by cortisone in 165%, 11-deoxycorticosterone - 160-164%, cortisole in 157% and prednisolone in 149%, whereas ß-estradiol had a stimulatory influence of 133-138% and testosterone – 119-121% in comparison to 100% control during the whole period of 20 days of Wolffia arrhiza cultivation.

17

Nieodwzajemniona fascynacja - dlaczego glikokortykosteroidy w stanach nagłych nie zawsze spełniają nasze oczekiwania?

51%

Kalwas-Sliwinska M. , Bonecka J. , Degorska B. , Jurka P.

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nr 05

18

Oznaczanie hormonow steroidowych w moczu

44%

Kotlowska A. , Kamysz W.

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nr 1

1-8

19

Atopowe zapalenie skóry u psów - najnowsze wiadomości na temat rozpoznawania i leczenia

37%

Banovic F.

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nr 3